Genetic variation study on fifteen STR loci in isolated Slovenian "Inland Island" human populations of the Selska Valley Region.

This study was conducted to confirm preliminary anthropological research indicating the specificity of isolated Selska Valley populations and implement clear mapping of genetic distances between neighboring populations and similar "inland island" populations from the region. The sample consisted of 86 unrelated individuals born in the Selska Valley from the lowland villages (Bukovica, Sevlje, Dolenja Vas, Selca, Zelezniki and Zali Log) and the mountain villages (Podlonk, Prtovc, Spodnje Danje, Zgornja Sorica and Spodnja Sorica). The for mentioned 15 STR loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA) were analyzed and statistical analysis was applied to determine population-genetics and forensic parameters. The frequencies of 15 STR loci from isolated populations of Slovenian villages, Slovenia, Bosnian mountain villages and Bosnia and Herzegovina were analyzed to calculate genetic distances between them. Our results confirmed a similar genetic pattern between the Selska Valley mountain villages and Slovenian general population as well as Bosnian mountain villages and general Bosnian population. Even if the sample size was relatively small and examined populations were geographically isolated, observed genetic variation within the whole population was relatively high and comparable with neighboring populations. Additional analysis proved that the Selska Valley population is genetically closest to the Slovenian and Austrian populations. On the other hand, comparison with similarly patterned populations confirmed that this population could be recognized as "inland island" population in genetic terms.

Correlates of Frailty among Aging Residents of Upper Selska Valley Villages under Ratitovec Mountain.

Frailty, multi-system dysregulation following multiple life stressors, is associated with age and vulnerability to negative health. Our model posits that variables such as age and sex affect biocultural changes resulting from lifestyle and alter frailty. We assessed frailty with a four-factor index. We expand understanding of frailty by examining associations with demographic, health, and lifestyle factors in a Slovenian sample. Between 2008 and 2009, 40 residents of the Selska Valley, Slovenia aged 55 to 85 years (X = 72, SD = 7.24) participated in physical assessments, responded to the SF-36, and self-reported their own and family history of non-communicable diseases. Participants included 26 women (age 59-86) and 14 men (age 57-82). We used linear regression and t-tests to assess associations of these factors with frailty. Frailty was significantly positively associated with age, sex, length of residence in the village, and multiple self-reported health factors. Conversely, frailty was significantly negatively associated with height and showed a borderline significant association with diastolic blood pressure. Controlling for age and sex, significant associations remained between frailty and self-reports of health, along with painful and reduced activity levels. Frailty also interacts with lifestyle factors. Results suggest the model proposed by Walston and colleagues (2005) is a valid cross-cultural measure of frailty.

Maternal genetic heritage of Southeastern Europe reveals a new Croatian isolate and a novel, local sub-branching in the x2 haplogroup.

High mtDNA variation in Southeastern Europe (SEE) is a reflection of the turbulent and complex demographic history of this area, influenced by gene flow from various parts of Eurasia and a long history of intermixing. Our results of 1035 samples (488 from Croatia, 239 from Bosnia and 130 from Herzegovina, reported earlier, and 97 Slovenians and 81 individuals from Zumberak, reported here for the first time) show that the SEE maternal genetic diversity fits within a broader European maternal genetic landscape. The study also shows that the population of Zumberak, located in the continental part of Croatia, developed some unique mtDNA haplotypes and elevated haplogroup frequencies due to distinctive demographic history and can be considered a moderate genetic isolate. We also report seven samples from the Bosnian population and one Herzegovinian sample designated as X2* individuals that could not be assigned to any of its sublineages (X2a'o) according to the existing X2 phylogeny. In an attempt to clarify the phylogeny of our X2 samples, their mitochondrial DNA has been completely sequenced. We suppose that these lineages are signs of local microdifferentiation processes that occurred in the recent demographic past in this area and could possibly be marked as SEE-specific X2 sublineages.

The Selska valley Study of Health and Aging: unraveling senescence, stress and frailty.

In this project we use an age-stratified sample of 100 men and 100 women aged 55 years and older who were residents of the Selska valley in order to explore two health indices, allostatic load (AL), and frailty. AL assesses lifelong stress responses using commonly assessed and clinically meaningful aspects of physiology; our frailty index assesses current somatic well-being using 5 aspects of functioning. Both correlate with clinical morbidity, self-reported health, life style, health history, and well-being. Our research site includes 9 villages located in the isolated Selska valley. Given their relative isolation, residents of this region provide a natural experimental setting for assessing stress, frailty, morbidity, and senescence in a local isolate. This isolated alpine setting provides numerous advantages for continuing research on health, disease and senescence. Our fieldwork protocols include detailed health demographic and SES interviews, measurement of blood pressure, anthropometry, walking speed, strength/endurance, and collection of blood and saliva samples for determinations of hormones, plasma proteins, and lipids. In this paper, we present one segment of data for 41 participants on self-report health and use of prescription medications during our 2008-2010 survey. In general, most participants rate themselves as being in good to excellent health (34/41 = 85%). However, over 66% are taking medications for a chronic condition, with about 25% taking 4 or more medications.

Distribution of surnames and linguistic-cultural identities in Western Slovenia.

The following study compares the distribution of surnames in the populations of Western Slovenian municipalities, which were part of the Italian state until the end of the Second World War. The analyses have been carried out firstly by assessing the similarity of different populations to one another so as to verify possible aggregations; secondly, by figuring these relationships of similarity in a representation through unrooted tree (NJ); finally, by applying spatial autocorrelation to the distribution of surnames in the territory so as to highlight possible processes in the diffusion of people in the area under examination. On the whole, it seems likely that the population living in the area is geographically quite stable, although some differences may be noted depending both on the geography of the territory, particularly in the northern part, and on peculiar historical and socioeconomic conditions, which at the same time have characterized and modified the demographic structure of some of these municipalities.

Age and symptom experience at menopause in the Selska Valley, Slovenia.

OBJECTIVE: To assess age and symptom experience at menopause in a high-altitude population in the Selska Valley of Slovenia. DESIGN: In four mountain villages, all houses were approached and 80% of eligible residents were interviewed. Additional women were interviewed for comparison in the valley below. Age at interview ranged from 32.7 to 85.5 years, with a mean of 58.2 years. The majority of women (62%) were aged 40 to 65 years. RESULTS: Of the 58 women interviewed, 7 had undergone menopause by hysterectomy (12%). Recalled age at natural menopause ranged from 42 to 54, with a mean of 50.3 (SD 2.9). By probit analysis, median age at natural menopause was 52.03. Fifty-five percent of participants reported ever having experienced a hot flash, although only 24% reported hot flashes during the 2 weeks before being interviewed. When the sample was limited to women aged 40 to 65, frequency of hot flashes in the 2 weeks before the interview was 39%. For all participants, the most frequent complaint was lack of energy (66%), followed by backaches (59%), and joint stiffness (53%). CONCLUSIONS: In contrast to expectations, age at menopause was not earlier and hot flash frequency was not significantly lower at higher elevations.