In vivo assessment of specific cytotoxic T lymphocyte killing.

The direct killing of target cells by cytotoxic T lymphocytes (CTLs) plays a fundamental role in protective immunity to viral, bacterial, protozoan and fungi infections, as well as to tumor cells. In vivo cytotoxic assays take into account the interaction of target and effector cells in the context of the proper microenvironment making the analysis biologically more relevant than in vitro cytotoxic assays. Thus, the development, improvement and validation of in vivo methods are necessary in view of the importance of the results they may provide. We describe and discuss in this manuscript a method to evaluate in vivo specific cytotoxic T lymphocyte killing. We used as model system mice immunized with human recombinant replication-deficient adenovirus 5 (HAd5) containing different transgenes as the trigger of a CTL-mediated immune response. To these mice, we adoptively transferred syngeneic cells labeled with different vital fluorescent dyes. Donor cells were pulsed (target) or not (control non-target) with distinct CD8 T-cell epitopes, mixed in a 1:1 ratio and injected i.v. into immunized or non-immunized recipient mice. After 18-24h, spleen cells are collected and analysed by flow cytometry. A deviation from the 1:1 ratio of control and target cell populations indicates antigen specific lysis of target cells.

Diseño experimental de una prótesis parcial removible a extensión distal para medir in vivo el desplazamiento de la mucosa con la técnica de impresión de modelo modificado / Experimental design of a partial denture a distal extension to measure in vivo movement of the mucosa with printing technique modified mode

El propósito de este estudio fue diseñar una prótesis parcial removible (PPR) a extensión distal experimental para medir in vivo el desplazamiento de la mucosa de los rebordes edéntulos con la técnica de modelo modificado. Se diseñó, construyó y evaluó clínicamente la precisión de una PPR experimental con dispositivos para medir el desplazamiento de la mucosa en 5 pacientes Clase I de Kennedy mandibular. Las medidas de ambos rebordes fueron promediadas y comparadas estadísticamente usando el test de t de student. Los valores de significancia (p) igual o menor a 0.05 fueron considerados estadísticamente significativos. No se encontraron diferencias estadísticamente significativas entre las medias de las medidas obtenidas del reborde edéntulo derecho e izquierdo. Se demostró la precisión y efectividad del diseño de la PPR a extensión distal experimental para evaluar el desplazamiento de la mucosa de los rebordes edéntulos con la técnica de modelo modificado

The purpose of the study was to design an experimental distal extension removable partial denture (RPD) to measure the displacement of residual ridge mucosa with the altered cast impression. An experimental RPD was designed and constructed. It was clinically evaluated in 5 mandibular Kennedy Class I patients. Student's t test was used to determine the statistical significance of differences in the right and the left residual ridges groups. No statistically significant differences were observed in both groups (p < 0.05). The accuracy of the experimental distal extensión RPD to measure the displacement of residual ridges mucosa with the altered cast impression was demostrated