Assuntos
Antineoplásicos/farmacologia , Transformação Celular Neoplásica/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas Nucleares/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Animais , Antineoplásicos/uso terapêutico , Proteínas de Ligação a DNA/agonistas , Humanos , Proteínas Nucleares/agonistas , Proteína Tumoral p73 , Proteínas Supressoras de Tumor/agonistasRESUMO
We propose an RT-PCR primer system allowing a definite mRNA identification for different p73 (p53 homologue) isoforms identification. Using the system proposed the p73 expression was studied in 18 glioma samples (cell lines, biopsy samples, primary cultures). Most of the samples reveal no p73 expression or it is expressed simultaneously with its isoforms shown to suppress its activity. This expression pattern predisposes to cancerogenesis.
Assuntos
Biomarcadores Tumorais/análise , Neoplasias Encefálicas/química , Proteínas de Ligação a DNA/análise , Glioma/química , Proteínas Nucleares/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Proteínas Supressoras de Tumor/análise , Biomarcadores Tumorais/genética , Biópsia , Linhagem Celular Tumoral , Proteínas de Ligação a DNA/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Proteínas Nucleares/genética , RNA , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Células Tumorais Cultivadas , Proteína Tumoral p73 , Proteínas Supressoras de Tumor/genéticaAssuntos
Biomarcadores Tumorais/genética , Genes Supressores de Tumor/genética , Neoplasias/diagnóstico , Neoplasias/terapia , Oncogenes/genética , Reação em Cadeia da Polimerase/métodos , Ensaios Clínicos como Assunto , Terapia Combinada , Diagnóstico Diferencial , Humanos , Neoplasias/genética , PrognósticoAssuntos
Antivirais/uso terapêutico , Infecções por Citomegalovirus/fisiopatologia , Monitoramento de Medicamentos/métodos , Sequência de Bases , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/tratamento farmacológico , Primers do DNA , Humanos , Reação em Cadeia da PolimeraseRESUMO
A quantitative comparison of 4-primer PCR-diagnosticum potentials for detection of cytomegalovirus DNA in urine specimens in different methods for DNA isolation was carried out. Comparatively simple DNA isolation procedures were considered for express diagnosis in clinical conditions. A scheme of successive use of diagnostic preparations was proposed.
Assuntos
Citomegalovirus/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Urina/microbiologia , Sequência de Bases , Citomegalovirus/genética , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/urina , Infecções por Citomegalovirus/virologia , Primers do DNA , DNA Viral/isolamento & purificação , DNA Viral/urina , Humanos , Recém-Nascido , Dados de Sequência MolecularRESUMO
The results of polymerization chain reaction (PCR) and morphological study are compared. The presence of cytomegalovirus (CMV) DNA in the organs of dead children is demonstrated by PCR in 13 of 16 cases. In 3 of 13 cases structural changes typical for CMV infection were absent; slight symptoms of the damage produced by DNA-virus were present in 4 cases. The results indicate that there exist variants of CMV infection with a minimum manifestation of the infectious process. The presence of mutations in the CMV genome was revealed in 6 cases.
Assuntos
Infecções por Citomegalovirus/patologia , Citomegalovirus/isolamento & purificação , Autopsia , Citomegalovirus/genética , Diagnóstico Diferencial , Genoma Viral , Humanos , Lactente , Recém-Nascido , Mutação , Inclusão em Parafina , Reação em Cadeia da PolimeraseAssuntos
Escherichia coli/genética , Genes Bacterianos , Recombinação Genética , Genótipo , PlasmídeosRESUMO
Relative efficiencies of spontaneous Bacillus subtilis transformation for markers placed in different areas of the cell chromosome were studied. As donor of genetic material, an untransformable strain BD224 trpC2 thr5 rec4 was used during its early log-phase. It was found that for markers placed near points of origin and termination of the chromosome replication the relative transformation efficiencies are significantly lower than those in the case of transformation with DNA extracted from the same donor cells. If a contact of spontaneously released DNA with the recipient cells was delayed for about 60 minutes ("separate" experiment) this difference proved to be less pronounced for ade16 placed near the origin, but remained practically unchanged for metB placed near the termination point. The results obtained can be explained by permanent attachment of chromosome regions, carrying "origin" and "termination" points, to a cytoplasmic membrane. During spontaneously release of cellular genetic material, "origin" and "terminal" DNA fragments carrying ade16 and metB respectively, can retain the contact with components of cell membrane. Hence, their penetration in the recipient cell and (or) participation in recombination can be violated. The first of two fragments becomes free from structurating substances more easy.
