Synthesis of 5α-androstane-3α,17ß-diol 17-O-glucuronide histaminyl conjugate for immunoassays.

Simple method of preparation of 5α-androstane-3α,17ß-diol 17-O-glucuronide N-histaminyl amide was developed for the construction of immunoanalytical kit. Improved method of glucuronide derivative synthesis was used, followed by hydroxybenzotriazole-dicyclohexylcarbodiimide coupling with histamine.