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1.
Bull Exp Biol Med ; 153(1): 89-93, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22808502

RESUMO

The aim of this study was to create vector superparamagnetic nanoparticles for tumor cell visualization in vivo by magnetic resonance tomography. A method for obtaining superparamagnetic nanoparticles based on ferric oxide with the magnetic nucleus diameter of 12 ± 3 nm coated with BSA and forming stable water dispersions was developed. The structure and size of the nanoparticles were studied by transmissive electron microscopy, dynamic light scattering, and x-ray phase analysis. Their T2 relaxivity was comparable with that of the available commercial analog. Low cytotoxicity of these nanoparticles was demonstrated by MTT test on primary and immortalized cell cultures. The nanoparticles were vectorized by monoclonal antibodies to connexin 43 (Cx43). Specific binding of vectorized nanoparticles to C6 glioma Cx43-positive cell membranes was demonstrated. Hence, vector biocompatible nanoparticles with high relaxivity, fit for use as MRT contrast for the diagnosis of poorly differentiated gliomas, were created.


Assuntos
Meios de Contraste/química , Compostos Férricos/química , Glioma/patologia , Espectroscopia de Ressonância Magnética/métodos , Nanopartículas/química , Anticorpos Monoclonais/química , Linhagem Celular Tumoral , Humanos
2.
Bull Exp Biol Med ; 154(2): 274-7, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23330142

RESUMO

We developed a method for obtaining iron oxide nanoparticles and their conjugation with monoclonal antibodies to vascular endothelial growth factor. The resultant vector nanoparticles were low-toxic and the antibodies retained their immunochemical activity after conjugation. The study was carried out on rats with intracranial glioma C6 on day 14 after its implantation. The intravenously injected nanoparticles visualized the brain tumor in contrast to nanoparticles conjugated with nonspecific immunoglobulins that did not accumulate in the tumor.


Assuntos
Anticorpos Monoclonais/química , Anticorpos Monoclonais/imunologia , Glioma/patologia , Imageamento por Ressonância Magnética/métodos , Magnetismo , Nanopartículas/química , Fator A de Crescimento do Endotélio Vascular/imunologia , Animais , Meios de Contraste/química , Feminino , Ratos
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