[Description of the phylogenetic structure of hydrolytic prokaryotic complex in the soils].

With the help of the molecular-biological method of cell hybridization in situ (FISH), the abundance of a physiologically active hydrolytic prokaryotic complex in chernozem and gley-podzolic soils is determined. The total proportion of metabolically active cells, which were detected by hybridization with universal probes as representatives of the domains Bacteria and Archaea, in samples of the studied soil, was from 38% for chernozem up to 78% for gley-podzolic soil of the total number of cells. The differences in the structure of chitinolytic and pectinolytic prokaryotic soil complexes are detected. Along with the high abundance of Actinobacteria and Firmicutes in the soils with chitin, an increase in phylogenetic groups such as Alphaproteobacteria and Bacteroidetes is observed.

[Development of clinical radiology in the Military field therapy Department of the Military Medical Academy (the 90th anniversary of the birth of G. I. Alekseyev)].

On August 18, 2012, 90 years have passed since the birth of the former head of the Military field therapy Department of The Military-Medical Academy named after S. M. Kirov--the main radiologist of the Ministry of Defence of Russian Federation, the corresponding member of the Soviet Union Academy of Medical Science and the Russian Academy of Medical Science, the major- general of a medical service G. I. Alekseyev, who had been working in the department since its foundation till the last day of his life. Being the head of the department for twelve years, G. I. Alekseyev made a considerable contribution to the formation and development of native military radiology, training of medical and scientific skilled specialists. Professor G. I. Alekseyev's scientific ideas and views in the sphere of radiology were realized and developed in further educational, research and medical work of the department. Nowadays the staff of the Military field therapy Department remembers G. I. Alekseyev with special gratitude and appreciation and successfully realizes his ideas and plans in work.

[Modern possibilities of therapeutic aid in the time of mass sanitary losses of radiation profile].

Modern concept of medical-prophylactic measures in the time of mass radiation affection is presented in the present article. Moreover the past changes in organization-regular structure of medical service of the Armed Forces of the Russian Federation and appearance of new time-board equipment were taken into account. Recommendations about the rendering of all types of therapeutic aid on all stages of medical evacuation--first, premedical, medical assistance, qualified and specialized--are given.

[Rational antibacterial therapy of acute surgical diseases of abdominal organs].

Comparative analysis of medical documents, concerning 1055 patients, aged 18-92 yrs old, operated for an acute surgical diseases of abdominal organs in 2007-2009 yrs, was performed. Preoperative antibioticotherapy was performed in all the patients, in 877 of them ceftazidim (ceftadim) was administered as the main antibacterial preparation and in 178--other antibacterial preparations. Application of ceftadim, as a basic preparation in antibioticotherapy, had permitted to lower the purulent-septic complications rate by 61% and to reduce the stationary treatment duration of the patients.

[Thermophilic chitinolytic microorganisms of brown semidesert soil].

In brown semidesert soil, thermophilic prokaryotic organisms identified as Streptomyces roseolilacinus and Silanimonas lenta were shown to play the main role in chitin transformation at 50 degrees C. The phylogenetic positions of the isolated dominant chitinolytic microorganisms were determined on the basis of 16S rRNA gene sequencing. The consumption of chitin as a source of carbon and nitrogen by both the bacterium and the actinomycete was shown by considerable biomass accumulation, high emission of carbon dioxide, and presence in the medium of the chitinase exoenzyme.

[Methodological aspects of assessing chitin utilization by soil microorganisms].

A computational method for estimating specific activity of chitin decomposition by microorganisms is proposed. Spectrophotometric and gas chromatographic methods have been used to determine the rates of chitinase production, biomass accumulation, and carbon dioxide emission by pure cultures of microorganisms grown on a chitin-containing medium. Among dominants of the chitinolytic community of chernozem (Trichoderma viride, Stretomyces albolongus, Alcaligenes, and Arthrobacter), the highest chitinolytic activity is characteristic of prokaryotes. In brown desert-steppe soil, the main destructor prokaryotes are actinomycetes (S. roseolilacinus). The biomass of the fungus T. viride growth on the chitin-containing medium markedly exceeds that of prokaryotes, but the specific activity of respiration and chitinase production in actinomycetes S. roseolilacinus and S. albolongus is an order of magnitude higher than in T. viride.

[Temperature as an autoecological factor of chitinolytic microbial complex formation in soils].

The dynamics of carbon dioxide emission from soil was studied during chitinolytic succession induced by humidification and chitin introduction at different temperatures (5, 27, and 50 degrees C) using gas chromatography. The abundance and biomass of the chitinolytic bacterial and actinomycete complex in soil were evaluated by luminescent microscopy. Active development of the chitinolytic microbial complexes was observed at all studied temperatures. The most active growth of chitinolytic microorganisms was observed at high temperature during early succession and at low temperature during late succession. High and low temperatures provided for active development of the chitinolytic microbial complex in soils confined to warm climatic zones (brown desert-steppe soil) and soils of temporary zones (gray forest soil). Actinomycetes demonstrated the most active growth among chitinolytic microorganisms in the studied soil samples both at low and high temperatures..

[Clinical effectiveness of plasmapheresis and lymphocytoplasmapheresis in patients with systemic scleroderma]. / Klinicheskaia éffektivnost' plazmafereza i limfotsitoplazmafereza u bol'nykh sistemnoi sklerodermiei.

Clinical effect of plasma- and lymphoplasmapheresis in patients with various patterns of sclerodermia systematica was studied in 12 females with subacute and chronic course of the disease. The failure of the drug therapy preceded, the complications associated with corticosteroid treatment performed were the indications to the aforementioned procedures. The treatment course comprised 3-5 procedures with an interval of 2-3 days. During 1 plasmapheresis session 1000-1200 ml of the plasma were removed and 1100-1250 ml of the plasma and 4.5.10(9)-7.0.10(9) lymphocytes were removed by a session of lymphoplasmapheresis. The results obtained evidenced a positive influence of plasma- and lymphoplasmapheresis on the course of sclerodermia systematica, contributed to a decrease in the clinical manifestation of the disease and laboratory data indicating the activity of inflammation, led to a more rapid regression of the main symptoms of the disease than it was recorded during the routine treatment, promoted a longer remission and a significant reduction of the hormone dosage.