RESUMO
Microalgae possess the prospective to be efficiently involved in bioremediation and biodiesel generation. However, conditions of stress often restrict their growth and diminish different metabolic processes. The current study evaluates the potential of GABA to improve the growth of the microalga Chlorella sorokiniana under Cr (III) stress through the exogenous administration of GABA. The research also investigates the concurrent impact of GABA and Cr (III) stress on various metabolic and biochemical pathways of the microalgae. In addition to the control, cultures treated with Cr (III), GABA, and both Cr (III) and GABA treated were assessed for accurately analysing the influence of GABA. The outcomes illustrated that GABA significantly promoted growth of the microalgae, resulting in higher biomass productivity (19.14 mg/L/day), lipid productivity (3.445 mg/L/day) and lipid content (18%) when compared with the cultures under Cr (III) treatment only. GABA also enhanced Chl a content (5.992 µg/ml) and percentage of protein (23.75%). FAMEs analysis by GC-MS and total lipid profile revealed that GABA treatment can boost the production of SFA and lower the level of PUFA, a distribution ideal for improving biodiesel quality. ICP-MS analysis revealed that GABA supplementation could extend Cr (III) mitigation level up to 97.7%, suggesting a potential strategy for bioremediation. This novel study demonstrates the merits of incorporating GABA in C. sorokiniana cultures under Cr (III) stress, in terms of its potential in bioremediation and biodiesel production without disrupting the pathways of photosynthesis and protein production.
Assuntos
Chlorella , Microalgas , Biocombustíveis , Estudos Prospectivos , Proteínas/metabolismo , Microalgas/metabolismo , Biomassa , Lipídeos , Suplementos Nutricionais , Ácido gama-Aminobutírico/metabolismoRESUMO
The consumer demand for protein rich foods urges the exploration for novel products of natural origin. Algae can be considered as a gold mine of different bioactive compounds, among which protein is distributed in significant amounts i.e., around 30% and can even reach to 55-60% in some cyanobacteria. Bakery and dairy products are extensively consumed worldwide due to product diversification and innovation. However, incorporation of algae biomass can lead to the development of green colour and fishy flavour that usually is not accepted in such products. Therefore, isolation and application of algae-derived proteins opens a new door for food industry. The present review provides a comprehensive understanding of incorporation of algae as a protein-rich ingredient in bakery and dairy products. The paper provides a deep insight for all the possible recent trends related to production and extraction of algae proteins accompanied by their incorporation in bakery and dairy foods.
Assuntos
Ingredientes de Alimentos , Laticínios , Indústria Alimentícia , Indústria de Processamento de AlimentosRESUMO
A swarf of aluminum alloy with high corrosion resistance and ductility was successfully converted into fine hydro reactive powders via ball milling with silver powder and either lithium chloride or gallium. The latter substances significantly intensified particle size reduction, while silver formed 'cathodic' sites (Ag, Ag2Al), promoting Al corrosion in aqueous saline solutions with hydrogen generation. The diffraction patterns, microphotographs, and elemental analysis results demonstrated partial aluminum oxidation in the samples and their contamination with tungsten carbide from milling balls. Those factors were responsible for obtaining lower hydrogen yields than expected. For AlCl3 solution at 60 °C, Al-LiCl-Ag, Al-LiCl, Al-Ga-Ag, and Al-Ga composites delivered (84.6 ± 0.2), (86.8 ± 1.4), (80.2 ± 0.5), and (76.7 ± 0.7)% of the expected hydrogen, respectively. Modification with Ag promoted Al oxidation, thus providing higher hydrogen evolution rates. The samples with Ag were tested in a CaCl2 solution as well, for which the reaction proceeded much more slowly. At a higher temperature (80 °C) after 3 h of experiment, the corresponding hydrogen yields for Al-LiCl-Ag and Al-Ga-Ag powders were (46.7 ± 2.1) and (31.8 ± 1.9)%. The tested Ag-modified composite powders were considered promising for hydrogen generation and had the potential for further improvement to deliver higher hydrogen yields.
RESUMO
Two sorts of tablets were manufactured from ball-milled powder (aluminum scrap and copper) by cold pressing and spark plasma sintering. Their microstructure, phase, and elemental compositions were investigated via scanning electron microscopy, X-ray diffraction analysis, and energy-dispersive X-ray spectroscopy. New phases, Al2Cu and MgCuAl2, were detected in the samples. Their microstructure was formed by welded scrap particles, the intermetallides, and Cu-rich regions located majorly along 'interparticle boundaries' and, to a lesser extent, within small, micro- and nanosized 'intraparticle spots'. The tablets were sealed with adhesive, so only the top surface was exposed to the environment, and tested in a chlorine aqueous solution for hydrogen generation performance. For both sample sorts, hydrogen yields of nearly 100% were achieved. The sintered tablets reacted faster than the cold-pressed ones: at 60, 70, and 80 °C, their entire 'conversion into hydrogen' took ~80, 40, and 30 min. vs. ~220, 100, and 70 min. The experimental kinetic curves were fitted with a contracting geometry equation, and those for the sintered samples were approximated with higher precision. The key effect of the additive was to enhance hydrogen evolution through the galvanic corrosion of Al in the regions adjacent to the intermetallic inclusions and Cu-rich spots.