[Predicting the course of urgent abdominal diseases based on intensity of catabolic phenomena]. / Prognozirovanie techeniya urgentnykh zabolevanii zhivota na osnove otsenki intensivnosti katabolicheskikh yavlenii.

OBJECTIVE: To develop a highly informative method for predicting the course of early postoperative period in urgent abdominal surgery based on indicators of lipid metabolism. MATERIAL AND METHODS: We analyzed 113 patients with acute surgical abdominal disease including 56 (49.6%) ones with acute appendicitis complicated by peritonitis, 23 (20.4%) ones with acute intestinal obstruction complicated by peritonitis and 34 (30.0%) patients with acute moderate pancreatitis (early phase). Leukocyte count, malondialdehyde, medium-weight molecules and lipid composition (phospholipid lysoforms) were analyzed throughout a 5-day period. Considering these data, we developed a method for predicting the course of early postoperative period (patent). RESULTS: Original method is highly effective in predicting the course of early postoperative period in urgent abdominal diseases. Sensitivity and specificity of this method for acute abdominal diseases complicated by acute peritonitis are 94.7% and >86.3%, for acute pancreatitis - 92.7 and 85.4%, respectively. CONCLUSION: Efficacy of original method is determined by analysis of catabolic phenomena, i.e. indicators of tissue destruction. Of course, assessment of endogenous intoxication whose toxins are components of catabolic (membrane-destructive) processes is essential.

[Renal distress syndrome in surgery and uronephrology: concept, pathogenesis, basics of prevention and correction]. / Renal'nyi distress-sindrom v khirurgii i uronefrologii: ponyatie, patogenez, osnovy profilaktiki i korrektsii.

OBJECTIVE: Based on objective criteria of the structural and functional state of the kidneys in various urgent surgical and uronephrological pathologies (peritonitis, pancreonecrosis, intestinal obstruction, urinary peritonitis, acute purulent secondary pyelonephritis) to identify and prove the general pattern of development of renal changes, their influence on the pathogenesis of homeostasis disorders at the organizational level and to form the evidence base of a new symptom complex - renal distress syndrome in surgery and uronephrology; to establish the effectiveness of Remaxol in its correction. MATERIAL AND METHODS: Experimentally on 60 mongrel dogs with acute peritonitis, pancreatitis, intestinal obstruction of varying severity, the state of renal function, including detoxification, was assessed based on the assessment of the inflow-outflow difference in the level of toxins and in the parenchyma of organs - the composition of lipids, the activity of peroxidation of membrane lipids and phospholipases. Clinical and laboratory studies included patients with acute peritonitis (48), acute pancreatitis (18), intestinal obstruction (21), acute purulent secondary pyelonephritis (19). Patients with peritonitis (20) underwent standardized therapy (the first subgroup) or with the inclusion of Remaxol (28) (the second subgroup). RESULTS: In the experiment and in the clinic, in acute surgical and some urological diseases with a different nature of the inflammatory process, there is a significant decrease in renal function. The recorded acute renal injury is combined with a pronounced membrane-stabilizing process in the tissue structures of the kidneys, the degree of which is associated with the severity of the disease. In order to correct and stabilize the indicated changes, Remaxol was used. The drug increased kidney tolerance to trigger pathogenetic agents, which improved the functional status of the kidneys. These data confirm the significance of the studied molecular mechanisms in kidney damage in urgent pathology. CONCLUSION: Experimental and clinical evidence has been obtained for the formation of a new concept - a symptom complex in acute surgical and uronephrological diseases of the abdominal cavity and retroperitoneal space called renal distress syndrome. Renal distress syndrome in surgery and uronephrology is a set of pathological processes of the body, the most important manifestation of which is the progression of endogenous intoxication due to a violation of the functional status of the kidneys as a result of membrane-stabilizing phenomena of organ cells due to oxidative stress and phospholipase activity.

Ferritin-based fusion protein shows octameric deadlock state of self-assembly.

Ferritin is a universal protein complex responsible for iron perception in almost all living organisms and has applications from fundamental biophysics to drug delivery and structure-based immunogen design. Different platforms based on ferritin share similar technological challenges limiting their development - control of self-assembling processes of ferritin itself as well as ferritin-based chimeric recombinant protein complexes. In our research, we studied self-assembly processes of ferritin-based protein complexes under different expression conditions. We fused a ferritin subunit with a SMT3 protein tag, a homolog of human Small Ubiquitin-like Modifier (SUMO-tag), which was taken to destabilize ferritin 3-fold channel contacts and increase ferritin-SUMO subunits solubility. We first obtained the octameric protein complex of ferritin-SUMO (8xFer-SUMO) and studied its structural organization by small-angle X-ray scattering (SAXS). Obtained SAXS data correspond well with the high-resolution models predicted by AlphaFold and CORAL software of an octameric assembly around the 4-fold channel of ferritin without formation of 3-fold channels. Interestingly, three copies of 8xFer-SUMO do not assemble into 24-meric globules. Thus, we first obtained and structurally characterized ferritin-based self-assembling oligomers in a deadlock state. Deadlock oligomeric states of ferritin extend the known scheme of its self-assembly process, being new potential tools for a number of applications. Finally, our results might open new directions for various biotechnological platforms utilizing ferritin-based tools.

Disjunct habitat of cryptic Terebellides (Annelida, Trichobranchidae) species shows a phylogenetic link between polychaetes from the White and the North Seas.

Understanding the distribution and biodiversity of marine species is crucial for developing effective conservation strategies and maintaining the health of global ecosystems. Advancements in molecular data utilization have significantly improved our understanding of biodiversity within the genus Terebellides. In this study, we conducted a phylogenetic analysis on polychaete samples from the Kandalaksha Bay, White Sea, revealing their affiliation with a putative undescribed species of the genus Terebellides found in two locations of the North Sea. Interestingly, this species was not detected in the Norwegian and Barents Seas, leading us to propose a disjunct distribution scenario for this Terebellides species. This unique distribution pattern might be attributed to the succession of polychaetes by new species, facilitated by the Gulf Stream and a climate change role in driving shifts in species' ranges and altering marine ecosystem dynamics.

[Enteral protection in patients with diffuse peritonitis]. / Enteroprotektsiya v povyshenii effektivnosti lecheniya patsientov s rasprostranennym peritonitom.

OBJECTIVE: To improve treatment outcomes in patients with diffuse peritonitis via enteral protection using intestinal lavage with ozonized solution through an original tube. MATERIAL AND METHODS: We analyzed 78 patients with advanced peritonitis. The control group included 39 patients who underwent standard measures after surgery for peritonitis. The main group consisted of 39 patients who underwent early postoperative intestinal lavage with ozonized solutions through original tube for 3 days. RESULTS: Clinical and laboratory parameters, as well as ultrasound data indicated better correction of enteral insufficiency in the main group. Morbidity in the main group was lower by 33.3%, length of hospital-stay - by 3.5 days. CONCLUSION: Early postoperative intestinal lavage with ozonized solutions through original tube accelerates recovery intestinal function and improves treatment outcomes in patients with widespread peritonitis.

[Optimization of early postoperative period in intestinal resection surgery for acute intestinal obstruction complicated by peritonitis]. / Optimizatsiya rannego posleoperatsionnogo perioda v rezektsionnoi khirurgii kishechnika pri ostroi kishechnoi neprokhodimosti, oslozhnennoi peritonitom.

OBJECTIVE: To establish the effectiveness of complex therapy with Remaxol in optimizing the early postoperative period and increasing the reparative ability of intestinal tissues in acute intestinal obstruction complicated by peritonitis. MATERIAL AND METHODS: We analyzed treatment outcomes in 37 patients with acute intestinal obstruction complicated by peritonitis. The control group included 19 patients who underwent standard therapeutic measures after resolving intestinal obstruction and resection of small or large intestine. The main group consisted of 18 patients who underwent intraoperative intestinal lavage with Remaxol through a probe and early postoperative intravenous infusions (800 ml within 2 days and 400 ml for subsequent 3 days). RESULTS: The main group was characterized by positive dynamics of clinical and laboratory parameters, in particular, relief of endogenous intoxication syndrome, reduction of oxidative stress and phospholipase activity, general hypoxia. Postoperative morbidity in the main group decreased by 61.7% (χ2=3.897, p<0.05). Better tissue healing in the area of intestinal anastomosis and laparotomy under Remaxol therapy was demonstrated. CONCLUSION: Inclusion of Remaxol in complex therapy of acute intestinal obstruction complicated by peritonitis can significantly improve treatment outcomes, reduce the number of complications and increase reparative potential of tissues. Positive effect of this drug is based on less oxidative stress, phospholipase activity and hypoxia.

Optogenetic cytosol acidification of mammalian cells using an inward proton-pumping rhodopsin.

Ion gradients are a universal form of energy, information storage and conversion in living cells. Advances in optogenetics inspire the development of novel tools towards control of different cellular processes with light. Rhodopsins are perspective tools for optogenetic manipulation of ion gradients in cells and subcellular compartments, controlling pH of the cytosol and intracellular organelles. The key step of the development of new optogenetic tools is evaluation of their efficiency. Here, we used a high-throughput quantitative method for comparing efficiency of proton-pumping rhodopsins in Escherichia coli cells. This approach allowed us to show that an inward proton pump xenorhodopsin from Nanosalina sp. (NsXeR) is a powerful tool for optogenetic control of pH of mammalian subcellular compartments. Further, we demonstrate that NsXeR can be used for fast optogenetic acidification of the cytosol of mammalian cells. This is the first evidence of optogenetic cytosol acidification by an inward proton pump at physiological pH values. Our approach offers unique opportunities to study cellular metabolism at normal and pathological conditions and might help to understand the role of pH dysregulation in cellular dysfunctions.

[Antibacterial therapy for secondary peritonitis at different ages]. / Antibakterial'naya terapiya pri vtorichnom peritonite v razlichnom vozraste.

The review is devoted to antibiotic therapy for secondary community-acquired and postoperative peritonitis in children and adults. The authors analyze the features of pathogenic microflora in secondary peritonitis at different ages, sensitivity of microorganisms to various antibacterial drugs. Particular emphasis is placed on the choice of antimicrobial drugs and various antibiotic regimens, especially when initial empiric treatment is prescribed.

[Correction of enteral distress syndrome in acute intestinal obstruction]. / Korrektsiya enteral'nogo distress-sindroma pri ostroi kishechnoi neprokhodimosti.

OBJECTIVE: On the basis of a comprehensive assessment of the functional state of the intestine in acute mechanical small bowel obstruction, to justify adequate schemes of its protection. MATERIAL AND METHODS: A clinical and laboratory study of 48 patients with acute small bowel obstruction developed against the background of abdominal adhesions, strangulated abdominal hernia was conducted. The first group (n=25) of patients who underwent laparotomy, removal of intestinal obstruction (adhesiolysis and/or herniation, hernial gate plastic surgery), intestinal intubation, standardized therapy after surgery. The second group (n=23) - patients, therapy included Remaxol (Polysan Pharmaceutical Plant, Petersburg): 400.0 enterally intraoperatively through a probe after nasointestinal intubation, evacuation of stagnant contents and intestinal lavage with isotonic saline solution; 400.0 - intravenously for 5 days. A number of indicators of homeostasis (endogenous intoxication, oxidative stress), structural and functional state of the intestine were evaluated. RESULTS: It was found that the inclusion of remaxol in complex therapy (intraoperatively and in the early postoperative period) leads to the optimization of the treatment process of patients with acute intestinal obstruction. The number of complications according to the Clavien-Dindo classification decreased from 17 (first) up to 5 (second group) (χ2=3.988, p=0.046). Hospital stay decreased from 12.8±1.1 to 10.1±0.8 bed days (p<0.05). The effectiveness of the developed scheme is based on its ability to correct the phenomena of enteral distress syndrome relatively quickly, which was confirmed by laboratory and instrumental methods. The most important manifestation of this was a significant decrease in the phenomena of endogenous intoxica tion against the background of a significant decrease in the activity of peroxidation of membrane lipids - triggers of catabolic intestinal lesions. CONCLUSION: Studies document the effectiveness of the developed treatment regimen for patients with acute intestinal obstruction. The inclusion of remaxol parenterally and enterally makes it possible to significantly optimize the course of the early postoperative period. One of the main objects of its implementation was the relatively rapid restoration of intestinal function, reduction of manifestations of enteral distress syndrome. This provided rapid relief of endogenous intoxication and, as a result, prevented the progression of the systemic inflammatory response syndrome, which together determined the optimization of the early postoperative period.

[Possible ways to approach the effectiveness of open abdominal interventions to laparoscopic]. / Vozmozhnye puti priblizheniya rezul'tativnosti otkrytykh abdominal'nykh vmeshatel'stv k laparoskopicheskim.

THE PURPOSE OF THE WORK: Based on the determination of the mechanisms of negative manifestations of surgical aggression in open interventions, compared with laparoscopic, to determine ways to optimize the early postoperative period when using them. MATERIAL AND METHODS: The work is based on clinical and laboratory analysis of 147 patients with acute appendicitis complicated by acute peritonitis. In the first group (n=58), patients were operated with traditional laparotomy access. In the second group (n=47) - laparoscopic interventions were used. In the first and second groups, traditional therapy was carried out according to clinical recommendations. In the third group (n=42), patients were operated with traditional laparotomy access, and in the early postoperative period, their therapy included remaxol (intravenous injections of 800.0 ml of the drug in the first two days, then within three days - 400.0 ml). Laboratory and instrumental studies were carried out in patients, including the level of hydrophilic and hydrophobic toxins in the blood, the intensity of peroxidation of membrane lipids and antioxidant enzyme potential, the activity of phospholipase A2. The functional status of the intestine and liver was assessed. RESULTS: It was found that the most important manifestation of the reduction of surgical aggression in the early stages after surgery, which is characteristic of laparoscopic operations, are significantly low phenomena of endogenous intoxication against the background of a significant decrease in the activity of peroxidation of membrane lipids and phospholipase systems - triggers of catabolic phenomena and lesions of various organs and systems, including the intestine and liver. The inclusion of remaxol in complex therapy for patients who have undergone open surgical operations leads to a number of positive clinical and laboratory effects. The most important of them is the reduction of endogenous intoxication. This beneficial effect is associated with the restoration of the functional status of the intestine and liver, as well as a decrease in the severity of catabolic phenomena. The effectiveness of complex therapy with remaxol was marked by reducing the number of postoperative complications and the length of the patient's stay in the hospital. CONCLUSION: The use of remaxol in patients with acute peritonitis complicated by peritonitis who have undergone open surgical interventions, due to the relatively rapid restoration of the functional status of the intestine and liver, reduction of catabolic phenomena, reduces the severity of endogenous intoxication syndrome, which was the basis for a significant improvement in the course of the early postoperative period, bringing it closer to that when using laparoscopic technology.

[The variants of enteral distress syndrome correction with peritonitis]. / Puti korrektsii enteral'nogo distress-sindroma pri rasprostranennom peritonite.

OBJECTIVE: To determine the effectiveness of a new treatment regimen for patients with widespread peritonitis with an accent on enteroprotection based on the use of a drug of the metabolic type of action of remaxol and physiotherapy. MATERIAL AND METHODS: Clinical and laboratory studies of 82 patients with widespread peritonitis, mainly with grade 2 severity according to the Mannheim peritonitis Index, severe form - according to the ARASNE II scale. In the 1st group (28) patients underwent intraoperative intestinal intubation in the traditional way; in the 2nd (22) - intestinal intubation according to the original method; in the 3rd (32) - intestinal intubation according to the original method and combined use of remaxol (parenterally, enterally and intraperitoneally). An intestinal tube of an original design was used, which allows electrical stimulation of the duodenum in the postoperative period. The study evaluates clinical data, the results of instrumental and laboratory studies: ultrasound examination of the abdominal cavity, assessment of the functional state of the small intestine, a number of indicators of homeostasis. RESULTS: It was shown that in patients with acute widespread peritonitis who underwent intestinal intubation according to the original method (group 2), as well as in combination with remaxol (group 3), the postoperative period was relatively more favorable. One of the factors optimizing the course of the disease was the relatively rapid restoration of the structural and functional state of the small intestine due to its electrical stimulation. The clinical and laboratory positive effect was especially significant in the 3rd group of patients, in which the use of remaxol, which has a membrane-stabilizing effect, played a special role in the enteroprotective effect. Timely correction of enteral distress syndrome resulted in a significant decrease in the severity of the syndrome of endogenous intoxication and oxidative stress, which was important in significantly improving the results of treatment of patients. CONCLUSION: The use of technical innovations in the form of an original nasointestinal tube, which allows to quickly restore intestinal motility due to electrical stimulation, as well as the use of remaxol (parenterally, enterally and intraperitoneally) significantly optimize the course of the early postoperative period of patients with acute widespread peritonitis.

[Endotoxin production in the early stages of urgent abdominal pathology and correction ways]. / Endogennaya intoksikatsiya v rannie sroki pri urgentnoi patologii organov bryushnoi polosti i puti ee korrektsii.

OBJECTIVE: To study the features of the development of endogenous intoxication in association with damage to the organs of the detoxification system, the severity of catabolic processes associated with the activity of peroxidation of membrane lipids and phospholipases in various urgent pathology of the abdomen, on the basis of which to develop a new pathogenetically based approach to the prevention of the progression of surgical endotoxicosis. MATERIAL AND METHODS: Clinical section. The studies were conducted in 162 patients with acute urgent pathology (seven groups) with predominant aseptic inflammation (acute severe pancreatitis, acute intestinal obstruction, acute catarrhal cholecystitis) and pronounced purulent-necrotic phenomena (acute peritonitis, acute destructive cholecystitis with empyema of the gallbladder). The severity of endogenous intoxication, the functional state of detoxification system organs (liver, kidneys and intestines), the activity of peroxidation of membrane lipids and phospholipases were evaluated in dynamics. In the last two groups of patients with pancreatitis and peritonitis, complex therapy included remaxol. Experimental section. The experiments were conducted on mongrel dogs that were modeled with acute peritonitis (n=15) or biliary pancreatitis (n=15). A complex of studies similar to those in the clinic was applied in dynamics, but in the tissue structures of the liver, kidneys and intestines, the activity of peroxidation of membrane lipids and phospholipases, as well as the phospholipid composition, was studied. RESULTS: Experimental and clinical studies have shown that in the studied diseases with a different nature of the inflammatory process (aseptic or purulent) in the initial periods, the production of toxins is largely associated with catabolic phenomena associated with a significant activation of peroxidation of membrane lipids and phospholipases. A noticeable increase in the activity of these processes was noted regardless of the nature and severity of the inflammatory process and is associated with the severity of endogenous intoxication and the dysfunctional state of the detoxification system organs. Subsequently, there is a decrease in the detoxification ability of the body against the background of stabilization or even reduction of catabolic phenomena. It is shown that a significant increase in the activity of trigger agents of membrane-stabilizing phenomena occurs regardless of the nature and severity of the inflammatory process. Their significant activity was experimentally established in the tissue structures of the detoxification system organs themselves, which caused the development of pronounced membrane-stabilizing phenomena. It has been shown that the use of remaxol in the complex therapy of patients leads to a noticeable decrease in the activity of membrane modulating agents, which leads to a significant decrease in the severity of endogenous inetoxification syndrome, regardless of the nature of inflammation. This fact was an additional evidence base for the conceptual rethinking of the significance of catabolic phenomena in the pathogenesis of endotoxin production in the early stages of the studied diseases. CONCLUSION: Experimental and clinical grounds have been obtained for a conceptual rethinking of the role of membrane-stabilizing agents in flooding the body with toxins at the beginning of the disease. One of the leading roles of peroxidation of membrane lipids and phospholipase systems in catabolic processes in the earliest periods of urgent abdominal diseases and in the defeat of detoxification system organs in the subsequent ones is shown.

Data on the synergistic effect of a hybrid filler based on graphene nanoplates and multiwalled nanotubes for increasing the thermal conductivity of an epoxy composite.

The dataset contains raw files related to the manuscript "The synergistic effect of a hybrid filler based on graphene nanoplates and multiwalled nanotubes for increasing the thermal conductivity of an epoxy composite" (Shalygina T.A. et al., 2021). The study presents the values of the heat capacity used to calculate the coefficients of thermal conductivity of epoxy composites by combining one-dimensional multiwalled nanotubes (MWCNTs) and two-dimensional graphene nanoplates (GNPs) in the role of a heat-conducting filler. To determine the heat capacity of materials with different concentrations of hybrid filler (GNP/MWCNT), the method of differential scanning calorimetry in the mode of the heat flux modulation was used. The analysis of the heat flux modulation samples is presented in raw and processed form. The materials scientists may apply the dataset to an in-depth study of the thermal conductivity formation mechanisms in composites doped with carbon-containing substances.

[Hepatic distress syndrome in surgery: concept, pathogenesis, prevention and correction]. / Gepaticheskii distress-sindrom v khirurgii: ponyatie, patogenez, osnovy profilaktiki i korrektsii.

OBJECTIVE: To establish the universality and regularity of hepatic disorders, their role in pathogenesis of homeostasis impairment and determine scientific base for a new syndrome - hepatic distress syndrome in surgery. MATERIAL AND METHODS: Chronic experiments on outbred dogs were carried out. Acute peritonitis was simulated in the first group (n=15), acute destructive pancreatitis in the second group (n=15), acute obstructive intestinal obstruction in the third group (15). In the fourth group, experimental acute peritonitis (n=15) was followed by Remaxol infusions (20 ml/kg). Liver function was evaluated using the indicators of endogenous intoxication, lipid metabolism markers, as well as composition of lipids in liver tissue. Clinical studies included 44 patients with peritonitis (the first subgroup - standard therapy (n=20), Remaxol infusion in the second subgroup (n=24), severe acute pancreatitis (n=18), acute adhesive intestinal obstruction (n=20)). Patients underwent surgery. Along with routine indicators, we analyzed severity of endogenous intoxication, lipid peroxidation, phospholipase activity, serum lipids and red blood cell count. RESULTS: Experimental and clinical studies have shown significant liver damage in all diseases with various pathogenesis. Regardless of urgent disease, one of the leading component is membrane-destabilizing process. The last one is determined by excessive activity of membrane lipid peroxidation and phospholipases in liver tissue. Severe abnormalities are followed by impairment of liver detoxification ability and liver may be a source of toxins per se. Remaxol infusion in the treatment of experimental and clinical acute peritonitis increased liver tolerance to trigger pathogenetic agents. This process resulted improvement of laboratory and clinical parameters. In general, we obtained high significance of liver damage in pathogenesis of acute urgent abdominal pathology. CONCLUSION: Experimental and clinical data for determining hepatic distress syndrome as important aspect in acute abdominal surgical diseases have been obtained. Hepatic distress syndrome in surgery is a combination of abnormal processes with progression of endogenous intoxication, oxidative stress and phospholipase activity following membrane-destabilizing phenomena and secondary liver dysfunction.

Chromatomembrane preconcentration of phenols using a new 3D printed microflow cell followed by reversed-phase HPLC determination.

Chromatomembrane process represents a universal approach to the separation of compounds in liquid-gas and liquid-liquid phases systems. However, the broad application of chromatomembrane separation methods in chemical analysis is restricted by the absence of serially produced chromatomembrane flow cells and the difficulties of their laboratory production. The present work addresses the preparation of chromatomembrane flow cell by using 3D printing. Fused deposition modeling and stereolithography were modes for the production of the flow cell using acrylonitrile-butadiene-styrene and polyacrylate-based Anycubic UV resins respectively. The separation and analytical performance of the 3D-printed flow cell were compared with a polyimide unit fabricated by a milling machine, the trial addressing the determination of phenol in the air. The method is based on chromatomembrane absorption of the analytes in 95 µL of the aqueous phase positioned in the cell. Reversed-phase HPLC with fluorimetric detection was applied for the determination of the absorbed analytes. The detection limit of phenols (phenol and m-cresol) in the air was 0.9 µg/m3 by absorption preconcentration time of 10 min. The volumetric flow rate of the analyzed air through the chromatomembrane cell using an electrodriven aspirator was 0.1 L/min.

[Systemic inflammatory response syndrome in pancreatic necrosis: triggers and organ damage]. / Sindrom sistemnogo vospalitel'nogo otveta pri pankreonekroze: triggernye agenty, organnye povrezhdeniya.

OBJECTIVE: To determine the main trigger mechanisms of multiple organ failure in acute severe pancreatitis. MATERIAL AND METHODS: An experimental study included 26 dogs with pancreatic necrosis. We assessed homeostasis disorders and functional changes in the pancreas, bowel, liver, kidneys, lungs and heart. Forty-six patients with severe acute pancreatitis were examined. We studied homeostasis disorders and functional state of the organs, endotoxemia, lipid peroxidation, phospholipase activity, coagulation system and hypoxia. RESULTS: Injury of various organs and systems due to systemic inflammatory response at the early stage of disease is an important aspect in progression of acute pancreatitis. Membrane destabilizing phenomena and disturbances in tissue component of coagulation system are the most significant factors. Patients with severe acute pancreatitis had significant changes in homeostasis. We distinguished two subgroups of patients. The course of disease was different. In the first subgroup, changes in homeostatic parameters were 15.4-24.2% less than in the second subgroup. This largely determined treatment outcomes as a whole. In the first subgroup, therapy was effective in most cases, in the second one - less effective that required surgical interventions. In the first subgroup, mortality and hospital-stay were less compared to the second subgroup. CONCLUSION: Oxidative stress, hypoxia, activation of phospholipases, and coagulation abnormalities are important in the development of systemic inflammatory response syndrome following acute pancreatitis. These factors are triggers for a cascade of the same kind of pathophysiological phenomena contributing to multiple organ failure and pancreatitis. In the tissues of various organs, proportional growth of these markers is observed until the 6th day, while in the blood - until the 4th day.

Data for determining the surface properties of carbon fiber in contact interaction with polymeric binders.

The data presented in this study are the supplementary materials related to the research paper "Determining the Surface Properties of Carbon Fiber in Contact Interaction with Polymeric Binders" (Voronina S.Yu. et al., 2018). The carbon fiber wettability properties before and after heat treatment and the coupling agent IR analysis results are presented. The coupling agent composition affects the fiber wettability and the capillary rise. The polymer binder impregnation rate drives the manufacturing process and the final composite quality. The data would be useful for researchers who study the interphase properties in composites and may help with determining the efficiency of applying certain polymers for wetting carbon fabrics.

[Factor analysis of clinical and paraclinical criteria determining the prognosis of acute renal damage].

AIM: to identify risk factors and assess their significance in the development and prognosis of acute kidney injury in urological patients. MATERIAL AND METHODS: The data of 6272 patients treated in the Department of Urology and Intensive care Unit were analyzed retro- and prospectively. Factorial and linear regression analyzes of the primary diagnostic criteria were carried out, which allowed to establish an impact of individual signs and their combination in determining the risk of developing or diagnosing existing renal dysfunction in the absence of clinical manifestations. RESULTS: Statistical analysis has demonstrated the high significance of some diagnostic criteria (gender, increased body mass index, duration of the disease, comorbidities, hyperthermia, epitheliuria, proteinuria) influencing the development of acute kidney injury in patients with urolithiasis complicated by renal colic or secondary pyelonephritis. The factor load of each criterion allowed to establish the worst prognosis and the risk of progression of acute kidney injury in patients with frequent recurrences of secondary pyelonephritis, episodes of renal colic over the last 5 years, a combination of arterial hypertension and diabetes mellitus who underwent extracorporeal shock-wave lithotripsy or pyelolithotomy with nephrostomy. These risk factors require for additional pharmacological protective therapy aimed at preventing the progression of acute kidney injury. CONCLUSIONS: Decreased urine output and serum creatinine levels as criteria for acute kidney injury (according to KDIGO) do not allow to fully assess the risk of renal dysfunction, which requires to consider additional criteria (gender, increased body mass index, duration of the disease, comorbidities, hyperthermia, epitheliuria, proteinuria) when taking history during the initial examination of a patient with upper urinary tract pathology.

[New technologies for stomach resection surgery in atypical conditions]. / Sovershenstvovanie rezektsionnoi khirurgii zheludka v nestandartnykh usloviyakh.

OBJECTIVE: To develop original technologies that simplify and increase an effectiveness of stomach resection in case of «difficult¼ duodenal ulcers. MATERIAL AND METHODS: Healing of gastroduodenostomy was experimentally observed under tension of anastomosed segments. In the first group (12), gastric resection was performed in a volume of 2/3 without tension of the anastomosed sections using a two-row suture. In the second group (12), stomach resection was accompanied by modeling of tension of the anastomosed sections under a pressure equal to 2.5-3.6 N (245-350 g). In the third group (12), gastric resection was performed in the same fashion as in the second group, but gastroduodenostomy was sutured using a single-row everted seams and hypotraction serous-muscular sutures. The results of Billroth-I gastrectomy using traditional (n=86) (classic double-row Lambert-Albert suture) and developed technologies (n=112) were analyzed in patients with complicated gastric and duodenal ulcers. RESULTS: The obvious advantages of the developed technologies have been experimentally proved for gastric resection under tension of anastomosed sections. There was no inconsistency of gastroduodenostomy, whereas traditional two-row suture was followed by this complication in 58,3% of cases. A favorable course was based on the relatively low inhibition of tissue blood supply in regenerating structures that was manifested by significantly less redox potential. Reduced lipid peroxidation and phospholipase activity in tissue structures along the suture line was also recorded. We obtained the positive clinical results of this approach in patients with complicated course of duodenal ulcer. Billroth-I gastrectomy was performed in all patients. Gastroduodenostomy failure was absent. Overall postoperative morbidity was 6,3%. In case of double-row anastomosis, suture failure occurred in 9,3% of cases, incidence of complications - 57,0%. This procedure was performed for gastric ulcer as a rule. CONCLUSION: The developed technologies greatly simplify resection of stomach and duodenum in the most difficult atypical conditions and ensure physiological Billroth-I surgery. The obvious benefit of this technology is simplified resection per se that makes it accessible to novice surgeons.