RESUMO
Polymorphism of chs_H1 gene encoding the "true" chalcone synthase was determined by alignment of sequences. The polymorphism associates with single nucleotide changes, insertions or deletions (indels) in the promoter, exons, intron, 3'-untranslated region. The molecular-genetic polymorphism in gene chs_H1 different regions of hop varieties of Polessye Agriculture Institute' breeding NAAS was analyzed.
Assuntos
Aciltransferases/genética , Genes de Plantas , Humulus/genética , Mutação INDEL , Polimorfismo de Nucleotídeo Único , Regiões 3' não Traduzidas , Sequência de Aminoácidos , Éxons , Expressão Gênica , Humulus/enzimologia , Íntrons , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Alinhamento de SequênciaRESUMO
Local alignment of Wx gene and its homolog sequences has been conducted. Phylogenetic dendrogram displaying evolutionary relationship between Poaceae members has been built basing on results of the alignment. Ancient Wx gene transfer from Zea mays to Dimeria lawsonii has been assumed. Primers for the exons 8-10 polymorphic region have been designed. In silico PCR has been conducted.
Assuntos
Biologia Computacional/métodos , Genes de Plantas , Filogenia , Proteínas de Plantas/genética , Polimorfismo Genético , Sintase do Amido/genética , Zea mays/enzimologia , Sequência de Bases , Éxons , Transferência Genética Horizontal , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Zea mays/genéticaRESUMO
The model of isogenous strains with chromosome substitutions has been used to estimate the relative contributions of the X chromosome and autosomes (chromosomes 2 and 3) to the control of some mating behavior traits in Drosophila melanogaster. It has been found that the male sexual activity (SA), female sexual receptivity (SR), and copulation latency (CL) are determined by interaction between X-chromosome and autosomal genes, whereas the copulation duration (CD) is mainly controlled by the X-chromosome genes. The synthesized isogenous strains have been shown to be more similar to hybrids than to the original strains. In the offspring with hybrid genotypes, the relationships between all traits are less stable, which may be related with an increase in the heterozygosity level and changes in genetic homeostasis.
Assuntos
Cruzamentos Genéticos , Locos de Características Quantitativas/fisiologia , Comportamento Sexual Animal/fisiologia , Cromossomo X/fisiologia , Animais , Drosophila melanogaster , Homeostase/fisiologia , Humanos , MasculinoRESUMO
The effects of mutations and genetic background on the mating activity of male and receptivity of female Drosophila melanogaster have been studied at different population densities. Population density, as well as its combinations with other factors, significantly affects mating behavior of D. melanogaster. There are two distinct trends in the effect of this factor on mating behavior: the maximum larval overpopulation may cause either a significant suppression of the behaviors studied or an increase in their expressivity. The mating behaviors of wa and cn mutants against a certain genetic background changed similarly in response to varying population density.
Assuntos
Genes de Insetos/genética , Mutação , Comportamento Sexual Animal/fisiologia , Animais , Drosophila melanogaster , Feminino , Genética Comportamental , Masculino , Densidade Demográfica , Reprodução/genéticaRESUMO
Components of mating behavior of Drosophila melanogaster mutant and wild-type strains were studied with respect to fitness. The magnitude of the effect of genotype on the male mating activity, female sexual receptivity, fertility and viability was determined. Strong positive correlation was found between the male mating activity and fitness components. It was shown that mating of strains contrasting in sexual behavior features can be accompanied by both heterosis and maternal effect. Inheritance coefficients were determined for sexual behavior components.
