[One-stage retrosternal colonic esophagoplasty in treatment of patients with scar burn stricture of esophagus].

Esophagoplasty was done in 23 patients an average of 10.4±2.3 months after chemical burn of esophagus. It was necessary to perform 29 corrective operations in 18 cases for training to one-stage plastic surgery such as gastrostomy, resection of stomach, retrocolic gastrojejunostomy on short loop. One-stage esophagoplasty was done in 21 of 23 cases. Functionally the most unusable anastomosis was cervical esophageal-intestinal anastomosis side-to-side. The number of complications after esophagocoloplasty by using of right flank was less than after using of left flank (39.92% vs. 50% respectively).

[Laparoscopic subtotal colectomy with mesorectumectomy in case of Hirschsprung's disease in adults].

Hirschsprung's disease in adults is a rare pathology. It was suggested the new method of Hirschsprung's disease surgical treatment in adults: distal subtotal colectomy, mesorectumectomy, appendectomy with ceco-anal anastomosis. The successful experience of laparoscopic subtotal colectomy, mesorectumectomy for this pathology is described in the article.

[The role of double-ballon enteroscopy in diagnosis and treatment of polypoid syndromes of gastrointestinal tract].

It was done 192 double-ballon enteroscopies in 141 patients at the period from March 2009 to March 2012. Epithelial benign tumors were detected in 10 (7.1%) patients with congenital polypoid syndromes. Endoscopic excision of polyps was done in 6 patients including 1 case with laparoscopic-assisted technique. Urgent open surgeries were performed before this in 4 patients with Peutz-Jeghers' syndrome and invaginated small bowel obstruction. The authors consider that double-ballon enteroscopy is highly informative diagnostic and treatment method in case of small bowel diseases. It allows to diagnose and to treat epithelial tumors on the early stage. Also it could help to avoid complications development and disability of patients.

[Choice of the method of surgical treatment of chronic pancreatitis].

An analysis of surgical treatment of 187 patients with chronic pancreatitis was made during 3-year period in the department of surgery clinic of Byelorussian Medical Academy of Post-Graduate Education. Drainage operations were performed on 28 patients, resection-drainage operations were carried out on 130 patients and resection operations had 19 patients. The laser beam technologies were successfully applied during operations on the pancreas in 43 patients. Postoperative complications (14.8%) were analyzed and structured. Methods of corrections and ways of prophylaxis of complication development were provided. On the basis of the complication analysis and new conception concerning peripheral pancreatic hypertension the authors offered the rational approaches to choice of operations on the pancreas in case of chronic pancreatitis. The authors developed the classification of pancreatoductolitiasis, pancreatic hypertension and a new strategy of surgical management of chronic pancreatitis.

[Improving the results of the intestinal obstruction treatment].

The results of surgical treatment of 517 patients with intestinal obstruction were analyzed. All patients had wide small bowel resections with primary anastomoses. The study demonstrated that the main survival predictive factors were: the age, general clinical condition, time before the operation, type of the anastomosis, resection volume of the adducent bowel loop and systemic complications. The increasing of the resectional volume and the use of "end-to-end" anastomoses lead to the statistically significant improvement of the treatment results.

[Accumulation of porphyrins in cells of system of blood induced by 5-aminolaevulinic acid].

The levels and rates of accumulation of porphyrins in lymphoid cells and bone marrow cells treated with exogenous 5-aminolaevulinic acid (ALA) were studied. The dependence of the quantity of porphyrins accumulated in cell on ALA concentrations in the medium had maximum at 0.7-1.0 mM ALA for all the cell types studied (splenocytes, thymocytes, peripheral blood lymphocytes and bone marrow cells). The rate of accumulation of uro-, copro- and protoporphyrins depended on cell types. The lowest and the highest levels were found in splenocytes and highest in bone marrow cells respectively. It is suggested that photodynamic therapy employing ALA is potentially dangerous for blood cells.

[Non-ulcer gastrointestinal bleedings].

Diagnostic and treatment protocol of non-ulcer gastrointestinal bleedings is defined. Special attention is paid to rare reasons of gastrointestinal bleedings, such as false aneurisms of the celiac trunk vessels. Applying diagnostic protocol and using endovascular techniques provide a dramatic improvement is treatment of such patients.

[Gastrointestinal bleedings of non-ulcer etiology].

During the period of 2001-2007 yy., more then 2300 patients with gastrointestinal bleedings were treated. 53% of them were diagnosed with duodenal or gastric ulcer disease. The developed diagnostic algorithm provided the correct diagnose and treatment in 98,2% of patients. Advantages of miniinvasive and endovascular methods by treating of gastrointestinal bleedings were demonstrated.

[Photodamage to spores of Fusarium fungi, sensitized by protoporphyrin IX].

The influence of photodynamic action with protoporphyrin IX as a sensitizer on the state of the components of hydrated spores of Fusarium fungi and germination of conidia in growth medium was investigated. It was shown, that protoporphyrin IX in micromole concentrations sensitizes the photooxidation of proteins and lipids from hydrated spores of Fusarium poae and Fusarium culmorum under illumination of their suspensions in doses of 50 - 200 kJ/m2. It was found that the photosensitized oxidation of cellular components leads to the disturbance of conidium membrane permeability and inhibition of spore germination during their subsequent cultivation in growth medium.

[Photodynamic inhibition of infection caused by herpes simplex virus type 1 in the cultured cells, by using 5-aminolevulinic acid-induced porphyrins].

On simulating infection caused by different herpes simplex virus type 1 (HSV-1) variants responsive and unresponsive to the drugs acyclovir and phosphonoacetic acid in the cultured Vero and C6 cells has revealed the higher ability of target cells to accumulate 5-aminolevulenic acid (ALA)-induced endogenous porphyrins, which determines the selectivity of their photo damages. Optimal conditions have been defined for all the studied HSV-1 variants to show a virus-inhibiting effect upon photodynamic exposure of infected and ALA-treated cell cultures.

[The possibilities of early diagnosis of tumorous lesions of colon patency and their preoperative correction].

Experiments in white rats were used in order 1) to create a model of obturative colon obstruction (OCO) and to prove a staged character of its development depending on the tone of the Bauhin's valve (the normotonic phase, spasm of the Bauhin's valve, dilatation of the Bauhin's valve) and 2) to study specific features of healing primary anastomoses after emergency resections of the colon. An analysis of clinical and rentgenological findings has confirmed the staged character of tumor lesions of the colon patency (TLCP). A working classification of them is proposed: stages of compensation (conventionally chronic); subcompensation (subacute) with three degrees of stages according to the experimental scheme of pathogenesis; decompensated (acute obturative colon obstruction). Typical rentgenological symptoms are first described and systematized for the stages of sub- and decompensation. In combination with the classification they make the basis for early diagnosis of TLCP, establishment of absolute indications for urgent operations and decision for the early intraoperative strategy. An original three-lumen probe for preoperative decompression and monitory purgation of the intestine, the method of intra- and postoperative decompression, correction of the intraintestinal status allowed to form primary anastomoses after urgent resections of the colon in 170 (63.7%) out of 317 patients with OCO. A scheme of control with the help of thermo-transducer is proposed for prognosis of a possible failure of the anastomosis.

[The influence of halogenorganic compounds on radiation induced damage of rat eye lens components]. / Vliianie galogenorganicheskikh soedinenii na radiatsionno-indutsiruemye povrezhdeniia komponentov khrustalika glaza krysy.

In experiments with white rats the damages of eye lens components of the animals are investigated under combined action of halogenorganic compounds (chloroform and sodium trichloroacetate) and radiation (137Cs) in vivo. It was shown that the injection of these compounds into animals amplifies the radiation-induced oxidation of eye lens components. The effect is manifested in the increase in the content of chromophors with fluorescence in the visible range and products of lipid peroxidation, the decrease in the amount of reduced glutathione. The data support the increased risk of development of radiation-induced cataract in the conditions of tehnogenic pollution of the environment.

Photodynamic inhibition of infection caused by drug-resistant variants of herpes simplex virus type I.

Membranotropic amphiphilic chromophore merocyanine 540 sensitized photodynamic inhibition of drug-resistant and sensitive variants of type I herpes simplex virus in cultured Vero cell. Optimal conditions of photodamage to virus particles and infected cells were determined (merocyanine 540 concentration 1 microM, illumination dose 32.5-65.0 kJ/m(2), exposure at early stages of infection). Infected cells actively bind the photosensitizer, which explains their selective photodamage.

[Aggravation by halomethanes of damages in isolated erythrocyte membranes photosensitized by porphyrins]. / Usilenie galogenmetanami fotosensibiliziruemykh porfirinami povrezhdenii izolirovannykh éritrotsitarnykh membran.

It was found that halogen methanes (CCl4, CHBr3) enhance the destruction of protein tryptophanyls and lipid peroxidation photosensitized by Zn-tetraphenylporphin and Zn-tetramethylpyridylporphyrin in isolated erythrocyte membranes. It was shown that this effect is due to photoinduced electron transport from Zn-porphyrins to halogen methanes with the formation of highly reactive halogenmethyl radicals. The hydrophilic Zn-tetramethylpyridylporphyrin is more active at the photosensition of damage to membrane proteins, whereas the hydrophobic Zn-tetraphenylporphin is more effective in lipid peroxidation.

[Mechanisms of enhancing UV lihg-induced lipid peroxidation in isolated erythrocyte membranes by organohalogen compounds]. / Mekhanizmy usileniia galogenorganicheskimi soedineniiami indutsiruemogo UF-svetom perekisnogo okisleniia lipidov v izolirovannykh membranakh éritrotsitov.

It was found that a number of halogen-containing compounds (chloroform, carbon tetrachloride, difluorodichloromethane, sodium trichloroacetate) enhance the lipid peroxidation induced by UV light (265-300 nm) in isolated erythrocyte membranes. It was shown that the enhancement is realized with participation of excited states of membrane protein tryptophanyls, which may induce the reduction of halogen-containing compounds with formation of radicals having a high oxidative activity. Moreover, halogen-containing compounds decrease the antioxidant protection of membrane lipids owing to an increase in quantum yield of alpha-tocopherol photodestruction.

[Effects of halogen derivative hydrocarbons on radiation damage of biological membranes]. / Vliianie galogenproizvodnykh uglevodorodov na radiatsionnye povrezhdeniia biologicheskikh membran.

Sharp increase of radiation-induced damages of isolated erythrocyte membrane lipids and proteins upon irradiation in presence of some halogen derivative hydrocarbon are shown. On chloroform and CCl4 examples it is shown that marked effects displayed under relatively low (submillimoles) concentrations and radiation doses that induced only insignificant oxidative membrane damages in absence of modifiers. The data are received which evidenced in favour of decisive role in these processes of halogenated organic radicals forming under one-electron reduction of halogen derivative hydrocarbons by hydrated electrons.

[Formation and photosensitizing activity of protein tryptophanile photochemical breakdown products in isolated erythrocyte membranes]. / Obrazovanie i fotosensibiliziruiushchaia aktivnost' produktov fotokhimicheskogo razrusheniia triptofanilov belkov v izolirovannykh éritrotsitarnykh membranakh.

Modification of photochemical processes in protein tryptophaniles of isolated erythrocyte membranes by chloroform was shown. It is established that formed in the presence of chloroform photoproducts are able to act as "internal" sensitizers of oxidation of membrane proteins and lipids when illuminated by UV light of ecological range.