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1.
Behav Brain Res ; 319: 31-36, 2017 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-27845227

RESUMO

Previous studies show that mice with Ywhae deficiency show abnormalities in brain development including defects in neuronal migration of post-mitotic pyramidal neurons as well as neuronal differentiation and proliferation in neuronal progenitor cells. Also, our previous research indicated that the Ywhae knockout mice show moderate defects in working memory and anxiety-like behavior. This previous work was performed using heterozygous mutant mice. Here we performed behavioral analyses using homozygous Ywhae knockout mice and found that the homozygous Ywhae knockout mice have increased locomotor activity, decreased working memory, and increased sociability. Taken together with the results obtained from the previous pathophysiological analyses in the Ywhae knockout mice, the Ywhae knockout mouse is useful for pathophysiological analyses of neuropsychiatric disorders caused by defects during neurodevelopment.


Assuntos
Proteínas 14-3-3/deficiência , Ansiedade/genética , Transtornos da Memória/genética , Proteínas 14-3-3/genética , Análise de Variância , Animais , Peso Corporal/genética , Modelos Animais de Doenças , Comportamento Exploratório/fisiologia , Feminino , Locomoção/genética , Masculino , Memória de Curto Prazo/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fatores de Tempo
2.
Neurosci Lett ; 628: 40-6, 2016 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-27288018

RESUMO

The 14-3-3 protein family is a group of multifunctional proteins that are highly expressed in the brain; however, their functions in brain development are largely unknown. Williams Syndrome is a neurodevelopmental disorder caused by a deletion in the 7q11.23 chromosome locus, including the gene encoding 14-3-3gamma, resulting in developmental delay, intellectual disabilities and epilepsy. We have previously shown that knocking down the 14-3-3gamma protein in utero in mice results in delays in neuronal migration of pyramidal neurons in the cortex. Importantly, there is a reciprocal duplication syndrome to Williams Syndrome where the 7q11.23 locus is duplicated, resulting in epilepsy and intellectual disabilities. Thus, the deletion or the duplication of the 7q11.23 chromosome locus results in epilepsy. Taken together with the fact that defects in neuronal migration are one of main causes for epilepsy, we analyzed if the overexpression of 14-3-3gamma causes neuronal migration defects. In this work, we found that the overexpression of 14-3-3gamma in utero in the developing mouse cortex results in delays in pyramidal neuron migration, similar to what was previously observed when 14-3-3gamma was knocked down. These results, in conjunction with our previous research, indicate that a balance of 14-3-3gamma expression is required during cortical development to prevent delays in neuronal migration. This work provides clear evidence as to the involvement of 14-3-3gamma in neurodevelopmental disorders and how a disruption in 14-3-3gamma expression may contribute to the neurodevelopmental disorders that manifest when the 7q11.23 locus is altered.


Assuntos
Proteínas 14-3-3/metabolismo , Movimento Celular , Córtex Cerebral/embriologia , Córtex Cerebral/fisiologia , Células Piramidais/fisiologia , Animais , Ciclo Celular , Córtex Cerebral/metabolismo , Camundongos , Camundongos Endogâmicos ICR , Células Piramidais/metabolismo
3.
Hum Mol Genet ; 25(20): 4405-4418, 2016 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-28173130

RESUMO

17p13.3 microduplication syndrome is a newly identified genetic disorder characterized by duplications in the 17p13.3 chromosome locus, resulting in a variety of disorders including autism spectrum disorder (ASD). Importantly, a minimum duplication region has been defined, and this region exclusively contains the gene encoding 14-3-3ε. Furthermore, duplication of this minimum region is strongly associated with the appearance of ASD in human patients, thus implicating the overexpression of 14-3-3ε in ASD. Using in vitro and in vivo techniques, we have found that 14-3-3ε binds to the microtubule binding protein doublecortin preventing its degradation. We also found that 14-3-3ε overexpression disrupts neurite formation by preventing the invasion of microtubules into primitive neurites, which can be rescued by the knockdown of doublecortin. To analyse the function of 14-3-3ε in neurite formation, we used 14-3-3ε flox mice and found that 14-3-3ε deficiency results in an increase in neurite formation. Our findings provide the first evidence of cellular pathology in 17p13.3 microduplication syndrome.


Assuntos
Proteínas 14-3-3/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Neuritos/metabolismo , Neuropeptídeos/metabolismo , Proteínas 14-3-3/genética , Animais , Transtorno do Espectro Autista/genética , Proteínas de Transporte , Deleção Cromossômica , Proteínas do Domínio Duplacortina , Duplicação Gênica , Humanos , Deficiência Intelectual/genética , Camundongos , Camundongos Endogâmicos ICR , Proteínas Associadas aos Microtúbulos/genética , Microtúbulos/metabolismo , Morfogênese , Neurônios/metabolismo , Neuropeptídeos/genética , Ligação Proteica
4.
Dev Neurobiol ; 76(6): 600-14, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26297819

RESUMO

14-3-3 proteins are ubiquitously-expressed and multifunctional proteins. There are seven isoforms in mammals with a high level of homology, suggesting potential functional redundancy. We previously found that two of seven isoforms, 14-3-3epsilon and 14-3-3zeta, are important for brain development, in particular, radial migration of pyramidal neurons in the developing cerebral cortex. In this work, we analyzed the function of another isoform, the protein 14-3-3gamma, with respect to neuronal migration in the developing cortex. We found that in utero 14-3-3gamma-deficiency resulted in delays in neuronal migration as well as morphological defects. Migrating neurons deficient in 14-3-3gamma displayed a thicker leading process stem, and the basal ends of neurons were not able to reach the boundary between the cortical plate and the marginal zone. Consistent with the results obtained from in utero electroporation, time-lapse live imaging of brain slices revealed that the ablation of the 14-3-3gamma proteins in pyramidal neurons slowed down their migration. In addition, the 14-3-3gamma deficient neurons showed morphological abnormalities, including increased multipolar neurons with a thicker leading processes stem during migration. These results indicate that the 14-3-3gamma proteins play an important role in radial migration by regulating the morphology of migrating neurons in the cerebral cortex. The findings underscore the pathological phenotypes of brain development associated with the disruption of different 14-3-3 proteins and will advance the preclinical data regarding disorders caused by neuronal migration defects.


Assuntos
Proteínas 14-3-3/deficiência , Movimento Celular/fisiologia , Córtex Cerebral/citologia , Córtex Cerebral/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento/genética , Neurônios/fisiologia , Proteínas 14-3-3/genética , Fatores Etários , Animais , Animais Recém-Nascidos , Bromodesoxiuridina/metabolismo , Movimento Celular/genética , Eletroporação , Embrião de Mamíferos , Antígeno Ki-67/metabolismo , Camundongos , Camundongos Transgênicos , RNA Mensageiro , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo
6.
J Neurosci ; 34(36): 12168-81, 2014 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-25186760

RESUMO

During brain development, neural progenitor cells proliferate and differentiate into neural precursors. These neural precursors migrate along the radial glial processes and localize at their final destination in the cortex. Numerous reports have revealed that 14-3-3 proteins are involved in many neuronal activities, although their functions in neurogenesis remain unclear. Here, using 14-3-3ε/ζ double knock-out mice, we found that 14-3-3 proteins are important for proliferation and differentiation of neural progenitor cells in the cortex, resulting in neuronal migration defects and seizures. 14-3-3 deficiency resulted in the increase of δ-catenin and the decrease of ß-catenin and αN-catenin. 14-3-3 proteins regulated neuronal differentiation into neurons via direct interactions with phosphorylated δ-catenin to promote F-actin formation through a catenin/Rho GTPase/Limk1/cofilin signaling pathway. Conversely, neuronal migration defects seen in the double knock-out mice were restored by phosphomimic Ndel1 mutants, but not δ-catenin. Our findings provide new evidence that 14-3-3 proteins play important roles in neurogenesis and neuronal migration via the regulation of distinct signaling cascades.


Assuntos
Proteínas 14-3-3/metabolismo , Córtex Cerebral/metabolismo , Células-Tronco Neurais/metabolismo , Neurogênese , Proteínas 14-3-3/genética , Actinas/metabolismo , Animais , Cateninas/metabolismo , Movimento Celular , Proliferação de Células , Córtex Cerebral/crescimento & desenvolvimento , Córtex Cerebral/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Células-Tronco Neurais/citologia , Células-Tronco Neurais/fisiologia , Neurônios/citologia , Neurônios/metabolismo , Neurônios/fisiologia , Ligação Proteica
7.
Biochem Biophys Res Commun ; 425(2): 212-8, 2012 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-22835934

RESUMO

Autosomal dominant polycystic kidney disease (ADPKD), the most common hereditary disease affecting the kidneys, is caused in 85% of cases by mutations in the PKD1 gene. The protein encoded by this gene, polycystin-1, is a renal epithelial cell membrane mechanoreceptor, sensing morphogenetic cues in the extracellular environment, which regulate the tissue architecture and differentiation. However, how such mutations result in the formation of cysts is still unclear. We performed a precise characterization of mesenchymal differentiation using PAX2, WNT4 and WT1 as a marker, which revealed that impairment of the differentiation process preceded the development of cysts in Pkd1(-/-) mice. We performed an in vitro organ culture and found that progesterone and a derivative thereof facilitated mesenchymal differentiation, and partially prevented the formation of cysts in Pkd1(-/-) kidneys. An injection of progesterone or this derivative into the intraperitoneal space of pregnant females also improved the survival of Pkd1(-/-) embryos. Our findings suggest that compounds which enhance mesenchymal differentiation in the nephrogenesis might be useful for the therapeutic approach to prevent the formation of cysts in ADPKD patients.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Cistos/prevenção & controle , Túbulos Renais/anormalidades , Mesoderma/efeitos dos fármacos , Rim Policístico Autossômico Dominante/prevenção & controle , Progesterona/administração & dosagem , Animais , Cistos/embriologia , Cistos/genética , Dilatação Patológica/embriologia , Dilatação Patológica/prevenção & controle , Feminino , Mesoderma/citologia , Camundongos , Camundongos Mutantes , Rim Policístico Autossômico Dominante/embriologia , Rim Policístico Autossômico Dominante/genética , Gravidez , Canais de Cátion TRPP/genética
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