Arsenic speciation in food in Belgium. Part 2: Cereals and cereal products.

This study reports results of total arsenic (Astot) and various As species in 75 samples of cereals and cereal products bought on the Belgian market. In addition to rice, the samples were wheat, pasta, bread and some breakfast cereals. The inorganic species arsenite (AsIII) and arsenate (AsV), and the organic As compounds dimethyl arsinate (DMA) and monomethyl arsonate (MA) were the only As species detected. Mean Astot was 0.150 ±â€¯0.089 mg kg-1 in rice and 0.012 ±â€¯0.008 mg kg- in the non-rice cereals. The inorganic arsenic fraction (Asi = AsIII + AsV) dominated in all samples and was in the range 55%-100%. Significantly higher Astot and Asi concentrations were observed in white rice and brown rice compared to Basmati rice. Within the group of non-rice cereals bread and pasta showed significantly lower concentrations compared to wheat. All 30 rice samples were conform to the European maximum limits for Asi, laid down in Commission Regulation (EU) 2015/1006. Although regulatory limits certainly can help to protect consumer health, our results suggest that the currently fixed European maximum levels are, in Belgium, not expected to have any impact on the human exposure to Asi, which is a known carcinogenic substance.

TEM and SP-ICP-MS analysis of the release of silver nanoparticles from decoration of pastry.

Metallic silver is an EU approved food additive referred to as E174. It is generally assumed that silver is only present in bulk form in the food chain. This work demonstrates that a simple treatment with water of "silver pearls", meant for decoration of pastry, results in the release of a subfraction of silver nanoparticles. The number-based size and shape distributions of the single, aggregated, and/or agglomerated particles released from the silver pearls were determined by combining conventional bright-field TEM imaging with semiautomatic particle detection and analysis. In addition, the crystal structure of the particles was studied by electron diffraction and chemical information was obtained by combining HAADF-STEM imaging with EDX spectroscopy and mapping. The TEM results were confirmed by SP-ICP-MS. The representative Ag test nanomaterial NM-300 K was used as a positive control to determine the uncertainty on the measurement of the size and shape of the particles.

Accumulation of atmospheric deposition of As, Cd and Pb by bush bean plants.

Bush bean (Phaseolus vulgaris) was exposed to atmospheric deposition of As, Cd and Pb in a polluted and a reference area. The atmospheric deposition of these elements was significantly related to the concentrations in leaves, stems and pods at green harvest. Surprisingly there was also a clear relation for As and Pb in the seeds at dry harvest, even though these seeds were covered by the husks. Root uptake of accumulated atmospheric deposits was not likely in such a short term experiment, as confirmed by the fact that soil pore water analysis did not reveal significant differences in trace element concentrations in the different exposure areas. For biomonitoring purposes, the leaves of bush bean are the most suitable, but also washed or unwashed pods can be used. This means that the obtained relationships are suitable to estimate the transfer of airborne trace elements in the food chain via bush bean.

Dietary cadmium intake by the Belgian adult population.

The aim of this study was to estimate the dietary cadmium (Cd) intake of the Belgian adult population, to compare this dietary Cd exposure to the tolerable weekly intake (TWI) recently established by the European Food Safety Authority (EFSA) and to determine the major food groups that contribute to dietary Cd exposure in Belgium. Food consumption data were derived from the 2004 Belgian food consumption survey (two 24 h recalls, 3083 participants). Cadmium concentrations in food items (n = 4000) were gathered from the control program of the Belgian Federal Agency for the Safety of the Food Chain for the period 2006-2008. Dietary intake per individual was calculated from consumption data and median Cd concentrations. The population mean, median and 95th percentile of the dietary intake values were 0.98, 0.85 and 2.02 µg kg⁻¹ body weight per week respectively. Two percent of the Belgian adult population has a dietary Cd intake above the recent TWI of 2.5 µg kg⁻¹ body weight established by EFSA in 2009. Cereal products and potatoes contribute for more than 60% to Cd intake.

Accumulation of trace elements in cattle from rural and industrial areas in Belgium.

Trace element concentrations were determined in meat, kidney and liver tissue of cattle, which had resided more than 18 months in areas historically contaminated by emissions from the non-ferrous metal industry or in regions with normal background metal contamination levels in Belgium. Trace element levels were determined by inductively coupled plasma-mass spectrometry. Lead, arsenic and cadmium concentrations in meat were low for all cattle. Concentrations in kidneys were, respectively, 1.8-, 2.2- and 2.5-fold higher in cattle from the contaminated areas compared to animals from reference areas. The European maximum level for cadmium in kidney was exceeded by 75% of the kidneys from contaminated areas and 47% of the kidneys from reference areas. Lead, arsenic and cadmium concentrations in bovine livers from contaminated areas were increased by a factor of 2.3. Zinc levels in livers and kidneys were within normal ranges, but 20% higher in the contaminated areas compared to reference areas. Copper levels in livers were high in all areas. Dairy cattle accumulated significantly higher levels of cadmium in kidneys and of lead in liver and kidneys than did beef cattle.

PCDD/Fs and dioxin-like PCBs in home-produced eggs from Belgium: levels, contamination sources and health risks.

This paper discusses the dioxin TEQ levels as determined by the chemically activated luciferase gene expression assay (CALUX) and by HRGC-HRMS in eggs, soils, faeces and kitchen waste samples obtained in the CONTEGG study. The samples were collected in each Belgian province at private homes and in small gardens where chickens are held. The CALUX levels for eggs sampled in autumn were higher than the levels in eggs obtained at the same locations in spring (median values of 5.86 and 4.08 pg CALUX TEQ/g fat, respectively). The total WHO-TEQ levels in eggs, determined by HRGC-HRMS, ranged from 3.29 to 95.35 pg TEQ/g fat in autumn and from 1.50 to 64.79 pg TEQ/g fat in spring. In the soils on which the chickens forage, levels of 2.51-11.35 pg I-TEQ/g in autumn and 2.00-7.86 pg I-TEQ/g in spring were found. The congener pattern of PCDD/Fs in the eggs, soils and faeces was dominated by OCDD, in addition to 1,2,3,4,6,7,8-HeptaCDD, OCDF and 1,2,3,4,6,7,8-HeptaCDF. The predominant dioxin-like PCBs were PCB118, PCB 105 and PCB 156. The dioxin-like PCBs contributed on average 47%, 14% and 20% to the total WHO-TEQ in eggs, soils and faeces, respectively. Kitchen waste samples were very low-contaminated with dioxin-like compounds. The present results showed a good agreement between egg and soil TEQ levels for PCDD/Fs but not for dioxin-like PCBs. This study showed that current soil levels found in some private gardens do not lead to egg levels below the current EU maximal level of 6 pg total TEQ/g fat for dioxins and dioxin-like PCBs. The consumers of the analysed eggs attained 5-79% of the tolerable weekly intake (TWI) of 14 pg TEQ/kg bw for dioxins and dioxin-like PCBs by exposure to their home-produced eggs only.

PCB and organochlorine pesticides in home-produced eggs in Belgium.

The level of polychlorinated biphenyls (PCB) and persistent organochlorinated pesticides (OC) in home-produced eggs was investigated in Belgium. The concentration of dichlorodiphenytrichloroethane (DDT) is above the norm for 17% of the eggs collected during the spring on 58 different locations. For PCB, aldrin, dieldrin, and chlordane, 3-5% of the samples are above the norm too. These levels are surprisingly high for compounds banned for about 30 years. Higher concentrations in home-produced eggs are expected compared to battery eggs because of contact with the environment and especially the soil. For ten selected locations, the concentration in soils, excreta and feed was measured, but no simple correlation between egg and feed or soil level could be established. Hexachlorohexane, endosulfan, endrin, methoxychlor and nitrofen were not detected in any sample.

Assessment of the chemical contamination in home-produced eggs in Belgium: general overview of the CONTEGG study.

This overview paper describes a study conducted for the Belgian Federal Public Service of Health, Food Chain Safety and Environment during 2006-2007. Home-produced eggs from Belgian private owners of hens were included in a large study aiming to determine concentration levels of various environmental contaminants. By means of the analyses of soil samples and of kitchen waste samples, obtained from the same locations, an investigation towards the possible sources of contaminants was possible. Eggs, soils, faeces and kitchen waste samples were checked for the presence of dioxins, PCBs (including dioxin-like PCBs), organochlorine pesticides, trace elements, PAHs, brominated flame retardants and mycotoxins. The study design, sampling methodology and primary conclusions of the study are given. It was found that in some cases dioxin-like compounds were present at levels that are of concern for the health of the egg consumers. Therefore, measures to limit their contamination in eggs, produced by hens of private owners, were proposed and deserve further attention.

Mycotoxin analyses in some home produced eggs in Belgium reveal small contribution to the total daily intake.

Low levels of deoxynivalenol (DON, range: 2.6-17.9 ng/g) and its metabolite de-epoxy-DON (DOM-1, range: 2.4-23.7 ng/g) were found in 20 home-produced egg samples collected in Belgium during autumn 2006 (from 10 breeders) and spring 2007 (same breeders). DON intake assessment showed that the consumption of these eggs may contribute to less than 1% of the provisional maximum tolerable daily intake of 1 microg/kg body weight established by FAO/WHO. None of the egg samples analyzed had quantifiable levels of zearalenone (ZEA), alpha-zearalenol, beta-zearalenol, ochratoxin A (OTA) and citrinin (CIT). Intake of DON, ZEA, OTA and CIT via the consumption of home produced eggs seems not to be a matter of concern. Despite this, we recommend to continue in screening other eggs allowing to increase the sample size and the subsequent conclusion for mycotoxin contamination in eggs. As home produced food is generally not submitted to any compliance control and may be consumed in large quantities by their producers and other household members, it is worthwhile to further pay attention to the quality of feed as well as the environment in which the hens live.

Cadmium in the food chain near non-ferrous metal production sites.

Dietary cadmium (Cd) exposure was estimated for adults living in Cd-contaminated areas close to non-ferrous metal plants and compared with dietary Cd exposure in the general Belgian adult population. To evaluate the contamination levels of locally produced food items, 35 fruit samples, 97 vegetable samples, 98 samples of potatoes and 53 samples of meat, liver and kidney of cattle, which had resided for more than 18 months in the contaminated area, were analyzed for Cd. Mean Cd concentrations in fruit and vegetables were 1.1- to 9-fold higher than in samples from other regions at ambient Cd levels. Mean Cd concentrations in bovine meat, liver and kidney were 2-fold higher compared to samples from animals in other regions of Belgium. The estimated dietary intake was 31.3 and 63.3 microg day(-1) for average and large consumers, respectively, in the contaminated area, compared to 17 and 38.3 microg day(-1), respectively, for the general adult population. Excessive consumption of locally produced food items in areas close to non-ferrous metal plants could result in Cd intake levels exceeding the provisional tolerable weekly intake (PTWI).

Contaminants in organically and conventionally produced winter wheat (Triticum aestivum) in Belgium.

A database has been compiled with the levels of important contaminants (mycotoxins, heavy metals and pesticides) measured from 2002 to 2005 in winter wheat (Triticum aestivum) grown in Belgium according to the organic and conventional farming systems. Assuming no further change in contaminant levels during cereal processing and during the preparation of foodstuffs, conservative intakes are estimated for the consumers of cereal-based products such as flour, bread, breakfast cereals, dough and pastry. The results show that for the consumer of organic foodstuffs, estimated daily intakes are 0.56 microg deoxynivalenol (DON), 0.03 microg zearalenone (ZEA), 0.19 microg Cd, 0.28 microg Pb and 0.0006 microg Hg kg(-1) body weight, taking into account the average contaminant levels in unprocessed grains and the average cereal products consumptions in Belgium. For the consumers of conventional foodstuffs, the corresponding estimated daily intakes are 0.99 microg DON, 0.06 microg ZEA, 0.17 microg Cd, 0.12 microg Pb and 0.0007 microg Hg kg(-1) body weight. In addition, it appears that for the consumers of conventional products, intakes of some post-harvest insecticides have to be taken into account (0.11 microg chlorpyriphos-methyl, 0.2 microg dichlorvos and 0.24 microg pirimiphos-methyl kg(-1) bw). When expressed as a percentage of the tolerable/acceptable daily intake (TDI/ADI), it seems that the corresponding estimated (conservative) intakes are the highest for DON (56% for organic and 99% for conventional cereal products), ZEA (16% for organic and 32% for conventional cereal products), and Cd (19% for organic and 17% for conventional cereal products), all other estimated intakes of contaminants (including pesticides) being lower than 10% of the TDI/ADI.