RESUMO
INTRODUCTION: The objective of this study was to evaluate the efficacy of the association of a proton pump inhibitor (omeprazole) with Ca(OH)(2) as intracanal medication in a rat model of periapical lesions. METHODS: Periapical lesions were induced on the first right mandibular molar tooth of 36 male Wistar rats (6 per group). After 28 days, the distal canal of each tooth was prepared, filled with the respective dressing (negative control group, PEG 400; positive control group, Ca(OH)(2) + PEG400; test group, Ca(OH)(2) + omeprazole + PEG 400), and sealed with amalgam for 15 or 28 days. Microbiological samples were taken in 3 periods: S1, after 28 days of lesion induction; S2, after the biomechanical preparation; and S3, after the medication (15 and 28 days). RESULTS: The radiographic and histologic analysis revealed that either Ca(OH)(2) or Ca(OH)(2) plus omeprazole dressings produced a reduction of periapical lesions at 28 days, when compared with the negative control group. The reduction of periapical lesions and inflammatory cell infiltration was visibly improved by associating omeprazole with Ca(OH)(2), with an increase of reparative bone areas. The microbiological assessment showed a significant decrease of colony-forming units count from S1 to S2 or S3 collecting times, but no differences were observed between the S2 and the S3 time-periods or among the experimental groups within the S3 period. Further bacterial characterization showed a possible selective activity of the medications. CONCLUSIONS: Our data showed that association of omeprazole with Ca(OH)(2) favored a superior repair of rat periapical lesions and seemed to display different selective activity over endodontic microbiota, in comparison with the conventional Ca(OH)(2) dressing.
Assuntos
Omeprazol/uso terapêutico , Periodontite Periapical/tratamento farmacológico , Inibidores da Bomba de Prótons/uso terapêutico , Irrigantes do Canal Radicular/uso terapêutico , Animais , Hidróxido de Cálcio/uso terapêutico , Contagem de Colônia Microbiana , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/microbiologia , Combinação de Medicamentos , Masculino , Periodontite Periapical/microbiologia , Radiografia , Ratos , Ratos Wistar , Preparo de Canal RadicularRESUMO
The FTIR and FT-Raman spectra of the gallium(III) complexes of 8-hydroxyquinoline (oxine) and 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol), were recorded and briefly discussed by comparison with the spectra of the uncoordinated ligands and with some related species.
Assuntos
Clioquinol/química , Clioquinol/metabolismo , Gálio/química , Gálio/metabolismo , Oxiquinolina/química , Oxiquinolina/metabolismo , Estrutura Molecular , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman , VibraçãoRESUMO
The infrared and Raman spectra of Na[FeEDTA(H(2)O)]x2H(2)O and Na(4)[(FeEDTA)(2)O]x3H(2)O (EDTA=tetra anion of ethylenediaminetetraacetic acid), two complexes proposed as adequate for iron supplementation, were recorded and analyzed in relation to its structural peculiarities. Some comparisons between the recorded spectra are also presented, and the characteristics of the carboxylate motions as well as those of the metal-to-ligand vibrations are discussed in detail.
Assuntos
Ácido Edético/química , Ácido Edético/metabolismo , Compostos Férricos/química , Compostos Férricos/metabolismo , Análise Espectral Raman , Vibração , Cristalografia por Raios X , Suplementos Nutricionais , Modelos Químicos , Espectrofotometria InfravermelhoRESUMO
The infrared and Raman spectra of the bis-chelated Zn(II) complexes of the amino acids glycine, alanine, valine, leucine, isoleucine and phenylalanine were recorded and analyzed in relation to its structural peculiarities. Some comparisons between the recorded spectra are also presented and the characteristics of the carboxylate motions as well as those of the metal-to-ligand vibrations are discussed in detail.