A long and stressful day: Photoperiod shapes aluminium tolerance in plants.

Aluminium (Al), a limiting factor for crop productivity in acidic soils (pH ≤ 5.5), imposes drastic constraints for food safety in developing countries. The major mechanisms that allow plants to cope with Al involve manipulations of organic acids metabolism and DNA-checkpoints. When assumed individually both approaches have been insufficient to overcome Al toxicity. On analysing the centre of origin of most cultivated plants, we hypothesised that day-length seems to be a pivotal agent modulating Al tolerance across distinct plant species. We observed that with increasing distance from the Equator, Al tolerance decreases, suggesting a relationship with the photoperiod. We verified that long-day (LD) species are generally more Al-sensitive than short-day (SD) species, whereas genetic conversion of tomato for SD growth habit boosts Al tolerance. Reduced Al tolerance correlates with DNA-checkpoint activation under LD. Furthermore, DNA-checkpoint-related genes are under positive selection in Arabidopsis accessions from regions with shorter days, suggesting that photoperiod act as a selective barrier for Al tolerance. A diel regulation and genetic diversity affect Al tolerance, suggesting that day-length orchestrates Al tolerance. Altogether, photoperiodic control of Al tolerance might contribute to solving the historical obstacle that imposes barriers for developing countries to reach a sustainable agriculture.

Metabolic and DNA checkpoints for the enhancement of Al tolerance.

Acidic soils are a major limiting factor for food production in many developing countries. High concentrations of soluble Al cations, particularly Al3+, inhibit cell division and root elongation in plants. Al3+ damages several biomolecules, including DNA, impairing gene expression and cell cycle progression. Notably, the loss-of-function mutants of DNA checkpoints may mediate Al tolerance. Furthermore, mitochondrial organic acids play key roles in neutralizing Al3+ within the cell and around the rhizosphere. Here, we provide knowledge synthesis on interactions between checkpoints related to mitochondrial organic acid homeostasis and DNA integrity mediating Al tolerance in land plants. These interactions, coupled with remarkable advances in tools related to metabolism and cell cycle, may facilitate the development of next-generation productive crops under Al toxicity.