RESUMO
Penicillium griseoroseum lectin was 80-fold purified by successive DEAE Sepharose anion exchange and Sephadex G-100 gel permeation chromatography. P. griseoroseum lectin exhibited haemagglutination activity towards protease-treated rabbit erythrocytes. It showed specificity towards various carbohydrates such as d-mannose, N-acetyl-d-glucosamine, mucins, and so forth. P. griseoroseum lectin was found as a glycoprotein with glycan content of 4.33%. Purified P. griseoroseum lectin is homodimeric having a molecular mass of 57 kDa with subunit molecular mass of 28.6 kDa. Haemagglutination activity of purified P. griseoroseum lectin was completely stable from 25°C to 35°C at a pH range of 6-7.5. Lectin activity was not influenced by divalent metal ions and denaturants. P. griseoroseum lectin manifested mitogenicity towards mice splenocytes and activity reached a peak at 75 µg/ml of lectin concentration. P. griseoroseum lectin in microgram concentrations stimulated proliferation of mice splenocytes. Thus, P. griseoroseum lectin exhibits potential mitogenicity, which can be exploited for further biomedical applications.
Assuntos
Lectinas/química , Lectinas/isolamento & purificação , Mitógenos/química , Mitógenos/isolamento & purificação , Penicillium/química , Animais , Carboidratos/química , Cátions/metabolismo , Proliferação de Células/efeitos dos fármacos , Quelantes , Glicoproteínas/química , Hemaglutinação , Concentração de Íons de Hidrogênio , Lectinas/farmacologia , Masculino , Camundongos , Mitógenos/farmacologia , Peso Molecular , Multimerização Proteica , Estabilidade Proteica , TemperaturaRESUMO
The binding of mitogenic lectins to the glycoconjugates on cell surface receptor triggers multitude of reactions involving different signal transduction pathways which ultimately results in cell proliferation. Since 1960 after the chance discovery of mitogenic property of lectins by Nowell, it has attracted more attention. The property of stimulation of mature lymphocytes aided the immunological investigations to study and analyse the mechanism of cell stimulation and division. Plant lectins are extensively studied for mitogenic activity and their widespread applications are also reported. During the past two decades, the study of biological properties of microbial lectins has increased tremendously. Their biological activities including mitogenic potential are equally applicable as that of plant lectins. The review mainly focuses on mitogenic potential of microbial lectins. It will provide a comprehensive view regarding distribution of this remarkable property among microbes including algae, fungi and bacteria, mechanisms and models of mitogenic stimulation, and also assays being employed to detect the mitogenic activity.