circIFT80 Functions as a ceRNA for miR-142, miR-568, and miR-634 and Promotes the Progression of Colorectal Cancer by Targeting ß-Catenin.

Colorectal cancer (CRC) is the third most common form of malignant tumor and is characterized by high rates of proliferation and metastases. Circular RNAs (circRNAs) are a form of noncoding and closed loop RNA molecules and play vital roles in the progression of various types of cancer in humans. Here, we used circRNA microarray sequencing technology to analyze the different circRNAs between CRC tissues and normal tissues and explore the role of circIFT80 in progression of colorectal cancer. In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. And then, we found circIFT80-positive correlation with CTNNB1 (ß-catenin) by sponging miR-142, miR-568, and miR-634 upregulated the gene expression. These miRNAs which targeted ß-catenin mRNA were confirmed by dual-luciferase reporter system and RNA-pulldown. In addition, xenograft tumor experiments showed that circIFT80 accelerated the tumorigenesis of CRC in vivo. In conclusion, our work reveals the impacts of circIFT80 as ceRNA in the progression of CRC, by which sponging miR-142, miR-568, and miR-634 enhanced the expression levels of ß-catenin and activation Wnt/ß-catenin pathway. Collectively, our data indicate that circIFT80 serves as an oncogene in CRC and represents a novel candidate for diagnosis and treatment.

miR-503 inhibits cell proliferation and induces apoptosis in colorectal cancer cells by targeting E2F3.

OBJECTIVE: Colorectal cancer (CRC) is one of the major healthcare problems worldwide. A lot of miRNAs are aberrantly expressed in CRC and involved in its development and progression. The purpose of this study was to investigate the expression and function of miR-503 in CRC. METHODS: miR-503 expression was detected in CRC tissues and cell lines by Quantitative real-time PCR. Cell proliferation was assessed by MTT assay. Cell apoptosis and cell cycle distribution were measured by flow cytometry. Moreover, luciferase reporter assay and western blot were performed to determine the potential target of miR-503 in CRC cells. RESULTS: miR-503 was significantly decreased in CRC tissues and cell lines in comparison with controls. Overexpression of miR-503 in CRC cells remarkably inhibited cell proliferation and induced apoptosis. Furthermore, E2F3 was identified as a direct target of miR-503 in CRC cells and down-regulation of E2F3 had a similar effect as miR-503 overexpression on CRC cells. In addition, the expression of E2F3 was negatively correlated with miR-503 level in CRC tissues. CONCLUSIONS: miR-503 inhibits cell proliferation and induces apoptosis by directly targeting E2F3 in CRC cells, indicating its potential application in CRC diagnosis and therapy.

[Plastic bronchitis associated with severe influenza A (H1N1) in children: a case report and review of the literature].

OBJECTIVE: to improve understanding of the pathological and clinical features, diagnosis and treatment of plastic bronchitis associate with influenza A (H1N1). METHODS: one case of plastic bronchitis associated with influenza A (H1N1) diagnosed and treated in our hospital in January 2010 was reported and 19 cases of plastic bronchitis reported in the literature were reviewed. RESULTS: we describe a 5-year-old Chinese Japanese boy presenting with cough for 2 days, gasping and fever for 1 day was admitted. Left lung atelectasis and pneumothorax were found on chest X-ray examination. Pathologically, plastic bronchitis was diagnosed after the endogenous foreign body was extracted by bronchoscopy and classified as type 1 cast. In addition, influenza A (H1N1) was confirmed by the swabs which showed positive for H1N1 nucleic acid. The condition was controlled and the patient was cured and discharged after 16-days' treatment with antiviral therapy, low-dose corticosteroids, and antibiotics. CONCLUSION: plastic bronchitis associated with influenza A (H1N1) is a rare life-threatening disorder. The diagnosis could be made based on pathological findings of the bronchial casts as well as the positive H1N1 nucleic acid detection. Bronchoscopic extraction of casts in plastic bronchitis is not only useful for early diagnosis but an effective therapeutic modality for the disease. Influenza A (H1N1) may be a cause of plastic bronchitis.

[Historical review of Portal Hypertension].

It has been more than 100 years since the nomenclature Portal Hypertension was put forward, during which the treatment of Portal Hypertension in medical circles experienced a gradual perfection. Reviewing the developmental progress can help to improve the treatment of Portal Hypertension.