Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella.

Tektins are microtubule inner proteins (MIPs) and localize at the inside lumen of doublet microtubules (DMTs) of cilia/flagella. TEKTIP1, a newly identified protein by cryo-electron microscopy (cryo-EM), is proposed to be localized at the center of the tektin bundle and hypothesized to recruit tektins or stabilize the bundle. However, the physiological role of TEKTIP1 is unknown. In this study, we generated Tektip1-knockout (Tektip1-/-) mice and showed that they were male subfertile primarily due to reduced sperm motility. A high percentage of sperm from Tektip1-/- mice showed moderately disorganized axoneme structures and abnormal flagellar waveforms. TEKTIP1 predominately interacted with TEKT3 among tektins. Loss of TEKTIP1 partially disturbed the organization of tektin bundle by mainly affecting the native status of TEKT3 and its interaction with other tektins. Collectively, our study reveals the physiological role and potential molecular mechanism of TEKTIP1 in axonemal structure and sperm motility, highlights the importance of MIPs in stabilizing DMTs, and suggests a potential relevance of TEKTIP1 deficiency to human asthenospermia. Tektip1-/- mice will be an excellent animal model to study the DMT organization of sperm flagella using cryo-EM in future.

Product-State Distributions of Three-Body Recombination in Zero-Collision-Energy 4He2-Alkaline-Earth-Metal Systems.

The distributions of product states after three-body recombination (TBR) of zero-collision-energy 4He2X systems, with X being 9Be, 24Mg, 40Ca, 88Sr, or 138Ba, are investigated in the hyperspherical representation by quantum mechanically solving the Schrödinger equation. It is found that the weakly bound (dimer) product states are preferentially populated for all of these cases, which could be understood from the joint effects of the lowest incident channel and the relatively long-range behavior of the corresponding nonadiabatic couplings among these lowest incident and shallow recombination channels. For the strongly bound products, since the flow is accessible in the small hyperradial region, their distributions are closely related to the behavior of the nonadiabatic couplings among the corresponding deep recombination channels. Particularly, our results indicate that the products are not always formed exclusively in the most weakly bound state when the scattering lengths among the reactants are relatively large and that there may exist a large fluctuation of the strongly bound products versus their binding energies in the universal region. In addition, the total TBR rates of these nonuniversal systems are also accounted for by the joint effects of the main adiabatic potentials and nonadiabatic couplings.

CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men.

BACKGROUND: Male infertility is a worldwide population health concern, but its aetiology remains largely understood. Although CFAP70 variants have already been reported in two oligo-astheno-teratozoospermia (OAT) individuals by sequencing, animal evidence to support CFAP70 as a credible OAT-pathogenic gene is lacking. METHOD: Cfap70-KO mice were generated to explore the physiological role of CFAP70. CFAP70 variants were detected in infertile men with OAT by whole exome sequencing and Sanger sequencing confirmation. Cfap70-truncated mice were further generated to explore the pathogenicity of the nonsense variant of CFAP70 identified in the proband. FINDINGS: Here, we demonstrate that Cfap70-KO mice are sterile mainly due to OAT and further identify a Chinese infertile man carrying a homozygous nonsense variant (c.2962C > T/p.R988X) of CFAP70. Cfap70-truncated mice lacking 5-8 tetratricopeptide repeats (TPRs) mimic the patient's symptoms. CFAP70 is required for the biogenesis of spermatid flagella partially by regulating the expression of OAT-associated proteins (e.g., QRICH2), assisting the cytoplasmic preassembly of the calmodulin- and radial spoke-associated complex (CSC), and controlling the manchette localization of axoneme-related proteins. Moreover, we suggest that CFAP70-associated male infertility could be overcome by intracytoplasmic sperm injection (ICSI) treatment. INTERPRETATION: Overall, we demonstrate that CFAP70 is necessary to assemble spermatid flagella and that CFAP70 gene could be used as a diagnostic target for male infertility with OAT in the clinic. FUNDING: This study was supported by the National Key Research and Development Project (2019YFA0802101 to S.C), Open Fund of Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education (to S.C), Central Government to Guide Local Scientific and Technological Development (ZY21195023 to B.W), and Basic Research Projects of Central Scientific Research Institutes (to B.W).

Diffusion-relaxation correlation spectrum imaging for predicting tumor consistency and gross total resection in patients with pituitary adenomas: a preliminary study.

OBJECTIVE: To evaluate the ability of diffusion-relaxation correlation spectrum imaging (DR-CSI) to predict the consistency and extent of resection (EOR) of pituitary adenomas (PAs). METHODS: Forty-four patients with PAs were prospectively enrolled. Tumor consistency was evaluated at surgery as either soft or hard, followed by histological assessment. In vivo DR-CSI was performed and spectra were segmented following to a peak-based strategy into four compartments, designated A (low ADC), B (mediate ADC, short T2), C (mediate ADC, long T2), and D (high ADC). The corresponding volume fractions ([Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text]) along with the ADC and T2 values were calculated and assessed using univariable analysis for discrimination between hard and soft PAs. Predictors of EOR > 95% were analyzed using logistic regression model and receiver-operating-characteristic analysis. RESULTS: Tumor consistency was classified as soft (n = 28) or hard (n = 16). Hard PAs presented higher [Formula: see text] (p = 0.001) and lower [Formula: see text] (p = 0.013) than soft PAs, while no significant difference was found in other parameters. [Formula: see text] significantly correlated with the level of collagen content (r = 0.448, p = 0.002). Knosp grade (odds ratio [OR], 0.299; 95% confidence interval [CI], 0.124-0.716; p = 0.007) and [Formula: see text] (OR, 0.834, per 1% increase; 95% CI, 0.731-0.951; p = 0.007) were independently associated with EOR > 95%. A prediction model based on these variables yielded an AUC of 0.934 (sensitivity, 90.9%; specificity, 90.9%), outperforming the Knosp grade alone (AUC, 0.785; p < 0.05). CONCLUSION: DR-CSI may serve as a promising tool to predict the consistency and EOR of PAs. CLINICAL RELEVANCE STATEMENT: DR-CSI provides an imaging dimension for characterizing tissue microstructure of PAs and may serve as a promising tool to predict the tumor consistency and extent of resection in patients with PAs. KEY POINTS: • DR-CSI provides an imaging dimension for characterizing tissue microstructure of PAs by visualizing the volume fraction and corresponding spatial distribution of four compartments ([Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text]). • [Formula: see text] correlated with the level of collagen content and may be the best DR-CSI parameter for discrimination between hard and soft PAs. • The combination of Knosp grade and [Formula: see text] achieved an AUC of 0.934 for predicting the total or near-total resection, outperforming the Knosp grade alone (AUC, 0.785).

Full-dimensional quantum mechanical study of three-body recombination for cold 4He-4He-20Ne system.

The increase of the number of the two-body recombination channels strongly challenges the numerical calculation of the accurate rates for the three-body recombination (TBR) process and its reverse process, collision-induced dissociation (CID), at ultracold temperatures. By taking the 4He-4He-20Ne collision system as an example, we have obtained the rates for its TBR and CID processes involving all four recombination channels, including the two-body states 4He2 (l = 0) and 4He20Ne (l = 0, 1, 2) with l the rotational quantum number. By using the adiabatic hyperspherical method, we have considered not only total angular momentum J = 0 but also J > 0 in the ultracold collision energies (E = 0.01 - 100 mK × kB). It is found that 4He2 (l = 0) is the major product after the TBR process in the ultracold limit (E ≤ 0.1 mK × kB). The TBR rate into 4He2 (l = 0) is nearly one order of magnitude larger than the sum of the other three products, 4He20Ne (l = 0, 1, 2). Moreover, the CID rates for the three 4He20Ne (l = 0, 1, 2) + 4He initial states are close to each other and are smaller than that for the 4He2 (l = 0) + 20Ne initial state. Additionally, we have, for the first time, performed the channel-resolved scattering calculation that can explain the above-mentioned findings quantitatively.

Comparison of the Nerve Regeneration Capacity and Characteristics between Sciatic Nerve Crush and Transection Injury Models in Rats.

Objective: To provide useful information for selecting the most appropriate peripheral nerve injury model for different research purposes in nerve injury and repair studies, and to compare nerve regeneration capacity and characteristics between them. Methods: Sixty adult SD rats were randomly divided into two groups and underwent crush injury alone (group A, n = 30) or transection injury followed by surgical repair (group B, n = 30) of the right hind paw. Each group was subjected to the CatWalk test, gastrocnemius muscle evaluation, pain threshold measurement, electrophysiological examination, retrograde neuronal labeling, and quantification of nerve regeneration before and 7, 14, 21, and 28 days after injury. Results: Gait analysis showed that the recovery speed in group A was significantly faster than that in group B at 14 days. At 21 days, the compound muscle action potential of the gastrocnemius muscle in group A was significantly higher than that in group B, and the number of labeled motor neurons in group B was lower than that in group A. The number of new myelin sheaths and the g-ratio were higher in group A than in group B. There was a 7-day time difference in the regeneration rate between the two injury groups. Conclusion: The regeneration of nerve fibers was rapid after crush nerve injury, whereas the transection injury was relatively slow, which provides some ideas for the selection of clinical research models.

Ultracold state-to-state chemistry for three-body recombination in realistic 3He2-alkaline-earth-metal systems.

The ultracold state-to-state chemistry for three-body recombination (TBR) in realistic systems has recently been experimentally investigated with full quantum state resolution. However, many detected phenomena remain challenging to be explored and explained from the theoretical viewpoints because this generally requires computational powers beyond state of the art. Here, the product-state distributions after TBR of 3He2-alkaline-earth-metal systems, i.e., after the processes of 3He + 3He + X → 3HeX + 3He with X being 9Be, 24Mg, 40Ca, 88Sr, or 138Ba, in the zero-collision-energy limit are theoretically studied. Two propensity rules for the distribution of the products found in current experiments have been checked, and the mechanism underlying these product-state distributions is explored. Particularly, two main intriguing transition pathways are identified, which may be responsible for the nonlinear distribution of the products vs their respective rotational quantum number. In addition, the TBR rates of these systems are also influenced by details of the interaction potential and relevant nonadiabatic couplings.

Scattering length scaling rules for atom-atom-anion three-body recombination of zero-energy 4He4He6Li- system.

The scattering length scaling rules for three-body recombination (TBR) of the 4He4He6Li- system in the zero-energy limit (E → 0) are investigated by considering various post-Born-Oppenheimer (post-BO) contributions to the standard BO potential of He-He interaction. It is found that these post-BO effects on the TBR rates that lead to different dimer products could be well fitted by the scattering length scalings Cab with a being the scattering length of the He-He interaction, and b and C the constants. It is interesting to find that the powers b about both the weakly and deeply bound dimer products are quite different from the well-known powers given by the scattering length scaling laws in the fields of ultracold neutral atomic gases. Such difference and the distinct powers associated with various dimer products are qualitatively accounted for by the properties of the major effective potentials and relatively long-range nonadiabatic couplings. Particularly, from these properties, the scaling powers are expected to be dependent on systems, but the power scattering length scaling behaviors to be universal, which has also been checked to apply to a wide range of a by tuning the He-He interaction.

Characterization of aerobic granules formed in an aspartic acid fed sequencing batch reactor under unfavorable hydrodynamic selection conditions.

Granules initiation and development is the backbone of aerobic granular sludge technology. Feed composition can notably affect initiation and development of aerobic granules, and yield aerobic granules with distinct microbial community, morphology and structure. This paper reports an unexpected formation of aerobic granules in an aspartic acid fed SBR under unfavorable hydrodynamic selection conditions. Detailed characteristics of these aerobic granules were investigated in terms of morphology, structure, bioactivity and EPS. The results showed that due to the absence of favorable hydrodynamic selection pressure, the formed aerobic granules had an irregular shape with a rough outline and loose internal structure, which was quite different from mature aerobic granules. Bacteria in these aerobic granules were mainly presented in the form of microcolony with calcium and ß-polysaccharides responsible for its mechanical stability. The high N/C ratio of aspartic acid enabled the enrichment of significant amount of nitrifiers within aerobic granules and thus resulted in high nitrification activity of these aerobic granules. The negatively charged and hydrophilic aspartic acid also induced the bacteria to secrete more exopolysaccharides for contributing to more neutral and hydrophilic surface of the aerobic granules, which was beneficial for aspartic acid capture. As a result, polysaccharides, rather than proteins, became the major components of EPS in these aerobic granules. This paper provides us a foundation to better understand the granulation potential of proteinaceous substrates that is frequently encountered in industrial wastewaters.

Relationship between the laminectomy extension and spinal cord injury caused by acute spinal shortening: goat in vivo experiment.

OBJECTIVE: To investigate the relationship between different types of laminectomy extension and spinal cord injury subsequent to acute spinal shorting after 3-column osteotomy in living goat model. METHODS: A total of 18 healthy goats were selected, and a procedure of bivertebral column resections and total laminectomy of T13 and L1 was completed followed by different laminectomy extensions under the somatosensory evoked potential (SSEP) monitoring. The samples were divided into three groups according to types of subsequent laminectomy extension. In the first group (enlarged resection of upper lamina group), laminectomy extension was performed on 10 mm caudal to T12; in the second group (equidistant enlarged resection of upper and lower lamina group), laminectomy extension was performed on 5 mm caudal to T12 and 5 mm cranial to L2 simultaneously; and in the third group (enlarged resection of lower lamina group), laminectomy extension was performed on 10 mm cranial to L2. The SSEP measured after vertebral resection was set as the baseline, and the SSEP decreased by 50% from the baseline amplitude and/or delayed by 10% relative to the baseline peak latency was set as positive results, which indicated spinal cord injury. Spinal column was gradually shortened until the SSEP monitoring just did not show a positive result. The shortened distance (ΔH) and the changed angle of the spinal cord buckling (Δα) were measured in each group. Neurologic function was recorded by the Tarlov scores at 2 days after the surgery. RESULTS: The safe shortening distances of three groups were 38.6 ± 1.2 mm, 41.5 ± 0.7 mm, 43.7 ± 0.8 mm, respectively; the corresponding changed angles of the spinal cord buckling were 62.8 ± 6.9°, 82.8 ± 7.5°, and 98.5 ± 7.0°. Significant differences of ΔH and Δα were found among the three groups by LSD multiple comparison test (P < 0.05). Strong correlation between ΔH and Δα was shown in each group by Pearson's correlation test. CONCLUSIONS: Different laminectomy extensions after 3-column osteotomy have different effects on the prevention of SCI caused by acute spinal shortening. The enlarged resection of lower lamina is superior to equidistant enlarged resection of upper and lower laminas which is superior to enlarged resection of upper lamina in preventing SCI. These slides can be retrieved under Electronic Supplementary Material.

Insight into the fenton-induced degradation process of extracellular polymeric substances (EPS) extracted from activated sludge.

Although EPS in microbial aggregates are importance in successful implementation of biological wastewater treatment systems, they also exhibit detrimental role on certain circumstance, such as excess sludge dewatering. Extensive efforts have been put into the disruption of EPS for improving the dewaterability of excess sludge and Fenton's reagent treatment has been demonstrated to be a very promising sludge conditioning method for EPS destruction. However, the information regarding detailed degradation process of EPS during Fenton's reagent treatment is limited. In this study, EPS were extracted from activated sludge and treated with different concentrations of Fenton's reagent. The physicochemical characteristic changes of EPS under different treatment were investigated in terms of components, EEM, molecular weight (MW), UV-Vis and FTIR. The results showed that EPS were prone to be disintegrated, but hard to be fully mineralized. Humic substances in EPS were more resistant to Fenton's reagent than other components. Low MW components of EPS were preferentially degraded prior to the disruption of high MW components. Besides, the disintegration of EPS into lower MW ones was accompanied by the formation of higher MW compounds caused by the bridge interaction of Fe ions. The cleavage of protein's backbone in EPS was mainly through destruction of amide II (N-H and C-N) in -CO-NH-. Fenton's reagent treatment also led to a significant increase of oxygen-containing functional groups in EPS molecules. This paper may pave a path to deeply understand the mechanisms of dewatering improvements of excess sludge by Fenton's conditioning.

Cold atom-atom-anion three-body recombination of 4He4HexLi- (x = 6 or 7) systems.

Atom-atom-anion three-body recombination (TBR) in mixed 4He and xLi- (x = 6 or 7) is investigated in the adiabatic hyperspherical representation by quantum mechanically solving the Schrödinger equation. The distributions of product states following these TBR processes are found to be relatively different for the two systems when the collision energy is less than roughly 0.6 mK × kB or 0.3 mK × kB for 4He4He6Li- and 4He4He7Li- systems, respectively, with kB being the Boltzmann constant. For 4He4He6Li- systems, the rate of recombination into (v=0) l = 04He6Li- molecular anions is the largest with v and l denoting the rovibrational quantum numbers, while the TBR rate that leads to the formation of l = 14He6Li- molecular anions is a little smaller than that of neutral 4He2 molecules. For 4He4He7Li- systems, neutral 4He2 molecules tend to be the most products, following the yields of l = 0 and 1 4He7Li- molecular anions. However, in spite of these distinctly different distributions, the products of molecular anions, the sum of l = 0 and 1 4HexLi- products, are relatively larger than that of neutral 4He2 molecules for both the two systems.

Evidence for kinesio taping in management of myofascial pain syndrome: a systematic review and meta-analysis.

OBJECTIVE: The aim of this study was to evaluate the effectiveness of kinesio taping for managing myofascial pain syndrome in terms of pain intensity, pressure pain threshold, range of motion, muscle strength and disability. DATA SOURCES: PubMed, EBSCO, ScienceDirect, Web of Science, Cochrane Library and Physiotherapy Evidence Databases were searched from database inception to November 2018. METHODS: Randomized controlled trials (RCTs) that used kinesio taping as the main treatment protocol for participants diagnosed with myofascial pain syndrome were included. Two reviewers independently screened articles, scored methodological quality using Cochrane risk-of-bias tool and extracted data. The primary outcomes were pain intensity, pressure pain threshold and range of motion at post-intervention and follow-up. The secondary outcomes were muscle strength and disability at post-intervention and follow-up. DATA SYNTHESIS: Meta-analyses of 20 RCTs involving 959 patients showed that kinesio taping was more effective than other treatments in reducing pain intensity (mean difference (MD) = 1.06 cm, 95% confidence interval (CI): -1.66 to -0.46 cm, P = 0.006) and increasing range of motion (standardized mean difference (SMD) = 0.26, 95% CI: 0.09 to 0.43, P = 0.003) at post-intervention. Kinesio taping was also superior to other non-invasive techniques in relieving pain intensity at follow-up (MD = -0.68 cm, 95% CI: -1.22 to -0.13 cm, P = 0.02). However, there was no detectable effect on disability or function. CONCLUSION: Statistical evidence showed that kinesio taping could be recommended to relieve pain intensity and range of motion for patients with myofascial pain syndrome at post-intervention.

Puerarin 6″-O-xyloside possesses significant antitumor activities on colon cancer through inducing apoptosis.

Puerarin 6″-O-xyloside (PRX) is a major compound found in the root of the Pueraria lobata (Willd.) Ohwi. The present study aimed to investigate the antitumor activity of PRX against colon cancer and examine its possible mechanism. In the present study, the anti-proliferative effects of PRX against colon cell lines (SW480, LoVo and HCT-116) were evaluated using a Cell Counting Kit-8 assay, and the half maximal inhibitory concentration values of the SW480, LoVo and HCT-11 cells were 37.114, 49.213 and 43.022 µg/ml, respectively. Furthermore, the apoptosis of SW480 cells was detected using flow cytometry with Annexin V-fluorescein isothiocyanate/propidium iodide staining. Subsequently, western blot analysis was performed to examine the expression of proteins associated with apoptosis, invasion and metastasis of tumors. The results showed that PRX possessed antitumor activity against colon cancer cell lines in a dose-dependent and time-dependent manner. In addition, PRX significantly upregulated the expression levels of cleaved (c)-caspase-3, c-caspase-9, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein and phosphorylated c-Jun terminal kinase, and downregulated the expression levels of Bcl-2, matrix metalloproteinase (MMP)-3, MMP-9 and vascular endothelial growth factor (P<0.01). Therefore, the present study demonstrated the PRX exerted antitumor activity against colon cancer cell lines and that the anticancer mechanisms of PRX may be associated with the induction of mitochondria-mediated intrinsic apoptosis, and inhibition of tumor invasion and metastasis. The present study provides a scientific basis for the clinical use of PRX for the treatment of colon cancer.

Imaging of guided waves using an all-fiber reflection-based NSOM with self-compensation of a phase drift.

A phase-resolved reflection-based near-field scanning optical microscopy (NSOM) technique with an original all-fiber configuration is presented. Our system consists of an intrinsically phase-stable common-path interferometer. The reflection from the waveguide input facet or from an integrated fiber Bragg grating is used as the reference beam. This arrangement effectively suppresses the phase drift caused by environmental fluctuations. By raster scanning a silicon atomic force microscope probe, we measure the complex near fields of the propagating and stationary waves in silicon nanowaveguides. Our robust, align-free, cost-effective, and shot-noise-limited near-field imaging technique paves the way for versatile optical characterizations of nanophotonic structures on a chip.

Dynamics of a single hole in the Heisenberg-Kitaev model: a self-consistent Born approximation study.

The magnetic properties of 4d and 5d transition-metal insulating compounds with the honeycomb structure are believed to be described by the Heisenberg-Kitaev model, which contains both the isotropic Heisenberg interaction J and anisotropic Kitaev interaction K. In this paper, we study the single-hole propagation of the t-J-K model in various magnetically ordered phases by the self-consistent Born approximation. We find that there are low-energy coherent quasiparticle (QP) excitations in all of these phases which appear firstly around the K point in the first Brillouin zone (BZ), but the bandwidths of these QPs are very small due to the hole-magnon coupling. Interestingly, in the zigzag phase relevant to recent experiments, though the QP weights are largely suppressed in the physical spectra in the first BZ, we find that they recover in the extended BZs. Moreover, our results reveal that the low-energy QP spectra are reduced with the increase of K.

[Advances in study of plant type â ¢ polyketide synthases].

Polyketides are a large class of natural products with notable structural diversity and different biological activities. They have essential pharmacological value for human health. In plants, the enzymes responsible for the formation of phenolic metabolites backbone structures are collectively known as type Ⅲ polyketide synthases (PKSs), which are the key enzymes for the polyketides biosynthesis. The PKSs catalyze a series of condensation reactions of two-carbon acetate units with an acyl starter. A brief overview of this group of enzymes, including their reaction mechanisms, function modification, expression regulation, molecular evolution, and recent interesting findings are presented here.

An Experimental Study on the Optimal Timing for the Repair of Incomplete Facial Paralysis by Hypoglossal-facial 'Side'-to-side Neurorrhaphy in Rats.

OBJECTIVE: To investigate the optimal timing for the repair of persistent incomplete facial paralysis by hypoglossal-facial 'side'-to-side neurorrhaphy in rats. METHODS: A total of 30 adult rats with crushed and bulldog-clamped facial nerve injury were randomly divided into 5 groups (n = 6 each) that were subjected to injury without nerve repair or with immediate repair, 2-week-delayed repair, 4-week-delayed repair, or 8-week-delayed repair. Three months later, the effects of repair in each rat were evaluated by facial symmetry assessment, electrophysiological examination, retrograde labeling, and axon regeneration measurement. RESULTS: At 3 months after injury, the alpha angle significantly increased in the group of rats with 4-week-delayed repair compared with the other four groups. Upon stimulation of the facial nerve or Pre degenerated nerve, the muscle action potentials MAPs were recorded in the whisker pad muscle, and the MAP amplitude and area under the curve in the 4-week-delayed repair group were significantly augmented at 3 months post-injury. Similarly, the number of retrograde-labeled motor neurons in the facial and hypoglossal nuclei was quantified to be significantly greater in the 4-week-delayed repair group than in the other groups, and a large number of regenerated axons was also observed. CONCLUSION: The results of this study demonstrated that hemiHN-FN neurorrhaphy performed 4 weeks after facial nerve injury was most effective in terms of the functional recovery of axonal regeneration and activation of facial muscles.