Homogeneously niobium-doped MoS2 for rapid and high-sensitive detection of typical chemical warfare agents.

Rapid detection of Chemical Warfare Agents (CWAs) is of great significance in protecting civilians in public places and military personnel on the battlefield. Two-dimensional (2D) molybdenum disulfide (MoS2) nanosheets (NSs) can be integrated as a gas sensor at room temperature (25°C) due to their large specific surface area and excellent semiconductor properties. However, low sensitivity and long response-recovery time hinder the pure MoS2 application in CWAs gas sensors. In this work, we developed a CWAs sensor based on in-situ niobium-doped MoS2 NSs (Nb-MoS2 NSs) via direct chemical-vapor-deposition (CVD) growth. Characterization results show that the high content of Nb elements (7.8 at%) are homogeneously dispersed on the large-area 2D structure of MoS2. The Nb-MoS2 NSs-based CWAs sensor exhibits higher sensitivity (-2.09% and -3.95% to 0.05 mg/m3 sarin and sulfur mustard, respectively) and faster response speed (78 s and 30 s to 0.05 mg/m3 sarin and sulfur mustard, respectively) than MoS2 and other 2D materials at room temperature. And the sensor has certain specificity for sarin and sulfur mustard and is especially sensitive to sulfur mustard. This can be attributed to the improvement of adsorption properties via electronic regulation of Nb doping. This is the first report about CWAs detection based on two-dimensional (2D) transition metal dichalcogenides (TMDs) sensing materials, which demonstrates that the high sensitivity, rapid response, and low limit of detection of 2D TMDs-based CWAs sensor can meet the monitoring needs of many scenarios, thus showing a strong application potential.

Investigation of MOF-derived humidity-proof hierarchical porous carbon frameworks as highly-selective toluene absorbents and sensing materials.

Carbon frameworks (CFs) derived from metal-organic frameworks (MOFs) have been produced as adsorbents of toluene. To further obtain optimum hierarchical porous carbon structure of CFs, different treatment temperatures were applied to a typical kind of MOFs (ZIF-8). The adsorption capacity of the toluene of hierarchical porous CFs obtained from ZIF-8 under 1100 °C (CF-1100, adsorption capacity of 208.5 mg/g) was higher than that of other carbonization temperature and MOFs. Impressively, the adsorbent CF-1100 also exhibited strong hydrophobicity, low desorption temperature, and good selectivity to toluene. The adsorption capacity decreased by only 10.4% under wet condition compared with the dry condition, standing on the top of the recently reported adsorbents. The impressive adsorption performance of CF-1100 is attributed to the larger specific surface area (1024 m2/g) and pore volume (0.497 cm3/g), newly generated micropores (pore width is 0.6-0.8 nm) and mesopores (pore width above 10 nm), and carbonaceous structure with higher degree of graphitization. Based on the adequate adsorption performance, CF-1100 coated quartz crystal microbalances as sensor also showed a high sensitivity of 0.4004 Hz/ppm and small relative standard deviations of 1.0745% for toluene sensing. This contribution provides a foundation for optimizing potential adsorbents and sensing materials for air pollution abatement.

Enhanced dimethyl methylphosphonate detection based on two-dimensional WSe2 nanosheets at room temperature.

Chemical warfare agents, particularly nerve agents such as sarin, are exceptionally harmful and incredibly perilous to people. Thus, the sensitive detection of these gases is indispensable for reducing the risk of chemical weapons. Herein, we fabricated a room-temperature chemiresistive gas sensor based on two-dimensional few-layer tungsten diselenide (WSe2) nanosheets, which were prepared through a facile liquid-phase exfoliation method. The WSe2-based sensor has demonstrated sensitive and selective detection of dimethyl methylphosphonate (DMMP), which is a well-known simulant of the nerve agent sarin. The sensor based on WSe2 nanosheets revealed a high response reaching 8.91% to 10 ppm DMMP with a fast response time of 100 s. Furthermore, the sensor displayed reliable stability, excellent selectivity, and a low theoretical limit of detection of about 122 ppb. The enhanced sensing performance of WSe2 nanosheets can be ascribed to the increase of the specific surface area, which provides more active adsorption sites for DMMP molecules, thereby facilitating the charge transfer process between DMMP molecules and WSe2 nanosheets. Overall, our results indicate that two-dimensional transition metal dichalcogenide materials have the potential for the design and fabrication of high-performance nerve agent sensing devices.

Lycorine hydrochloride regulates the malignant biological behaviors of liver cancer HCCLM3 cells through the circASH2L/miR-124-3p axis / 中国肿瘤生物治疗杂志

@#［摘要］ 目的：探讨盐酸石蒜碱（lycorine hydrochloride，LH）对肝癌HCCLM3 细胞恶性生物学行为的影响及其对circASH2L/ miR-124-3p轴的调控作用。方法：将HCCLM3细胞分为不同浓度LH处理组（LH-L、LH-M、LH-H组）和Con、si-NC、si-circASH2L、 LH+pcDNA、LH+pcDNA-circASH2L 组；以CCK-8 法、平板克隆形成实验、流式细胞术、细胞划痕实验和Transwell 小室实验分别 检测HCCLM3 细胞的增殖、克隆形成、凋亡、迁移和侵袭；qPCR法检测HCCLM3 细胞中circASH2L和miR-124-3p 的表达量；双荧 光素酶报告基因实验检测circASH2L 和miR-124-3p 的靶向关系；WB 法检测cleaved-caspase3、cleaved-caspase9、E-cadherin、 N-cadherin 蛋白表达量。结果：与Con 组比较，LH不同浓度组细胞增殖抑制率升高（P<0.05），凋亡率与cleaved-caspase3、cleavedcaspase9 、E-cadherin 蛋白水平升高（均P<0.05），miR-124-3p 的表达水平升高（P<0.05），克隆形成数与侵袭细胞数减少（均 P<0.05），划痕愈合率与N-cadherin 蛋白水平降低（P<0.05），circASH2L 的表达水平降低（P<0.05），且不同浓度组间比较差异有统 计学意义（P<0.05）。circASH2L 可负向调控miR-124-3p。与si-NC 组比较，si-circASH2L 组细胞增殖抑制率升高（P<0.05），凋亡 率与cleaved-caspase3、cleaved-caspase9、E-cadherin 蛋白水平升高（均P<0.05），划痕愈合率与N-cadherin 蛋白水平降低（均 P<0.05），克隆形成数与侵袭细胞数减少（均P<0.05）。与LH+pcDNA组比较，LH+pcDNA-circASH2L 组miR-124-3p 的表达水平 降低（P<0.05），细胞增殖抑制率降低（P<0.05），凋亡率与cleaved-caspase3、cleaved-caspase9、E-cadherin 蛋白水平降低（均 P<0.05），克隆形成数与侵袭细胞数增多（均P<0.05），划痕愈合率与N-cadherin 蛋白水平升高（均P<0.05）。结论：LH可通过调控 circASH2L/miR-124-3p 轴来抑制肝癌细胞HCCLM3 的增殖、迁移、侵袭并诱导其凋亡。

Comparative study on different therapies on patients with primary central nervous system lymphoma.

OBJECTIVE: To compare and analyze three therapies on patients with primary central nervous system lymphoma (PCNSL), aiming to provide evidences for future treatment and prognosis. METHODS: Clinical data of 26 cases of PCNSL with normal immune system confirmed by postoperative pathology were retrospectively analyzed. Among them there were six cases with operation only, nine cases with operation and radiotherapy, and 11 cases with operation, radiotherapy and chemotherapy, and their survival rate was compared as well. RESULTS: The survival time of patients with operation only, operation combined with radiotherapy and operation combined with radiotherapy and chemotherapy was 6-11 months, 15-24 months and 24-51 months, respectively. And their median survival time was only nine months, 21 months and 38 months, respectively. CONCLUSIONS: Operation combined with radiotherapy and chemotherapy can dramatically extend PCNSL patients' survival time, therefore, it can be regarded as the first-line therapy.

Clear Cell Renal Cell Carcinoma With Extensive Osseous Metaplasia: Report of a Rare Case.

A 48-year-old Chinese woman presenting with continuing dull pain in the right lower back and abdomen was found to have a tumor with extensive osseous metaplasia in the upper-middle pole of the right kidney. The excised neoplasm specimen revealed a 29 × 26 mm hard tumor with dense ossification. Histopathological examination of the tumor showed that it contained lamellar bone forming trabeculae intermingled and spherical or polygonal epithelial cells that contained slightly irregular nuclei with small nucleoli and abundant pink to clear cytoplasm. Clear cell renal cell carcinoma with extensive osseous metaplasia is a very rare occurrence.