RESUMO
Trichophyton interdigitale is generally deemed as an anamorph of Arthroderma vanbreuseghemii based on internal transcribed spacer (ITS) sequencing, but recently their anamorph/teleomorph connection should be cautioned based on ß-tubulin phylogeny. We report three siblings and one consulting doctor who developed kerion and tinea corporis after contact with domestic rabbits. Seven same strains were isolated from four patients and three regions of a sick rabbit. The ITS and D1/D2 sequences of our isolate were 99 % homologous to A. Vanbreuseghemii, while ß-tubulin sequence was 100 % identical to T. interdigitale. Our isolate was identified as T. interdigitale based on maximum likelihood analysis of ß-tubulin. Random amplified polymorphic DNA revealed that the band patterns of five isolated strains and another rabbit-derived strain WCH023 were identical for OPF-03 and OPF-12. Skin lesions of all patients resolved completely for 2- to 6-week therapy of oral terbinafine and topical 1 % bifonazole or 1 % terbinafine cream. This study demonstrates that T. interdigitale of rabbit origin can cause various types of human dermatophytosis by mild scratch. Terbinafine may be the first choice for dermatophytosis caused by T. interdigitale.
Assuntos
Tinha/diagnóstico , Tinha/epidemiologia , Trichophyton/isolamento & purificação , Zoonoses/diagnóstico , Zoonoses/epidemiologia , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Animais , Antifúngicos/administração & dosagem , Criança , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Feminino , Genótipo , Humanos , Imidazóis/administração & dosagem , Masculino , Pessoa de Meia-Idade , Naftalenos/administração & dosagem , Filogenia , Médicos , RNA Ribossômico/genética , Coelhos , Análise de Sequência de DNA , Homologia de Sequência , Irmãos , Terbinafina , Tinha/microbiologia , Tinha/patologia , Resultado do Tratamento , Trichophyton/classificação , Trichophyton/genética , Tubulina (Proteína)/genética , Zoonoses/microbiologia , Zoonoses/patologiaRESUMO
The diagnostic value of modified Chicago sky blue (CSB) stain and potassium hydroxide (KOH) mount for superficial mycoses was compared using fungal culture as gold standard. The sensitivity and screening time of the CSB stain were superior to the KOH mount. The CBS stain is simple, quick and reliable for diagnosing superficial mycoses.
Assuntos
Dermatomicoses/diagnóstico , Técnicas Microbiológicas/métodos , Coloração e Rotulagem/métodos , Hidróxidos/metabolismo , Compostos de Potássio/metabolismo , Sensibilidade e Especificidade , Fatores de Tempo , Azul Tripano/metabolismoRESUMO
There is limited information regarding the molecular epidemiology and antifungal susceptibilities of Candida isolates using the Neo-Sensitabs method in patients with vulvovaginal candidiasis (VVC). From August 2012 to March 2013, 301 non-pregnant patients aged 18-50 years with suspected VVC were prospectively screened at a teaching hospital in southern China. The vaginal isolates were identified by DNA sequencing of internal transcribed spacer and the D1/D2 domain. Antifungal susceptibility testing of seven antifungal agents was performed using the Neo-Sensitabs tablet diffusion method. Candida species were isolated from 186 cases (61.79â%). The most common pathogen was Candida albicans (91.4â%), followed by Candida glabrata (4.3â%), Candida tropicalis (3.2â%) and Candida parapsilosis (1.1â%). The susceptibility rates to C. albicans were higher for caspofungin, voriconazole and fluconazole than those for itraconazole, miconazole, ketoconazole and terbinafine (P<0.01). The resistance rates to C. albicans were 4.7, 6.5, 7.1, 7.6, 12.3, 27.7 and 74.7â% for caspofungin, miconazole, itraconazole, voriconazole, fluconazole, ketoconazole and terbinafine, respectively. No drugs tested apart from fluconazole exhibited differences in resistance between C. albicans and non-albicans Candida isolates. The results demonstrate that, using DNA sequencing, C. albicans is the most common isolate from Chinese patients with VVC. Caspofungin, voriconazole and fluconazole may be preferable to other azoles and terbinafine in the treatment of VVC.