Molecular cloning and potential role of DiSOC1s in flowering regulation in Davidia involucrata Baill.

Davidia involucrata Baill. (dove tree) is unique Tertiary relic plant in China, also known as 'living fossil' and 'giant panda'. The MADS-box family gene SOC1 is involved in the regulatory pathway that integrates flowering signals to promote flowering at the optimal time. In this study, we isolated and identified two dove tree SOC1 homologues, named DiSOC1-a and DiSOC1-b. These two sequences possess highly conserved domains MADS-box and SOC1-motif, as well as the semi-conserved region K-box. DiSOC1-a and DiSOC1-b were expressed at varying levels in all tested tissues of dove tree and shared high levels of expression in the flower buds. The expression tendencies of both genes in bract were initially upward and then downward and were highest in young bracts. Neither DiSOC1-a nor DiSOC1-b was expressed in immature leaves. Proteins encoded by DiSOC1-a and DiSOC1-b were located in the nucleus. In addition, ectopic overexpression of both genes in WT Arabidopsis promoted early flowering and the growth of the main bolt. Taken together, these results suggest that DiSOC1-a and DiSOC1-b are involved in the flowering initiation and the main bolt growth process of dove tree. Our results provide a foundation for horticultural breeding to control flowering time of dove tree.

Patch test in Chinese in Shanghai with cosmetic allergy to cosmetic series and products.

BACKGROUND: There are increasing cosmetic adverse reactions in China, mostly cosmetic contact dermatitis, with the development of its cosmetics industry. AIMS: To identify the allergens and cosmetics products responsible for cosmetic allergic contact dermatitis (CACD) in Shanghai. METHODS: Five hundred and sixty patients, including 342 suspected CACD patients from dermatological clinic and 218 cosmetics consumers with an allergic history, were patch tested with a cosmetic series (C-1000 series). And 154 patients patched with possible culprit cosmetics. RESULTS: Two hundred and forty-six subjects showed positive reaction to C-1000 series. Clinic patients had much higher positive rate than that of cosmetics consumers. Leading allergens were methylisothiazolinone (MI), methylisothiazolinone + methylchloro-isothiazolinone (MCI/MI), thimerosal, and the positive rates were significantly higher in patients than in cosmetic consumers (P < .001 for all). MCI/MI elicited positive patch test reactions in 73.5% of MI-positive patients. Of those patched with cosmetic products, 19 patients had 31 positive reactions, including 22 skin care products and 5 color decorating. CONCLUSIONS: Preservatives, especially MI, MCI/MI, and thimerosal, were the most common cosmetic allergens, and skin care products occupied the most allergic reaction of CACD. Patch testing is highly recommended for suspected CACD patients. Future comparative investigation with large sample size should benefit cosmetovigilance surveillance.