Probiotic Compounds Enhanced Recovery after Surgery for Patients with Distal Gastric Cancer: A Prospective, Controlled Clinical Trial.

BACKGROUND: Enhanced recovery after surgery (ERAS) for radical distal gastrectomy needs to be improved urgently. We investigated the effects of probiotic compounds (including Lactobacillus plantarum, L. rhamnosus, L. acidophilus, and Bifidobacterium animalis subsp.lactis) on enhance recovery after gastrectomy. METHODS: The patients in this prospective study were divided into probiotic group (PG group, n = 36) and placebo group (CG group, n = 38), taking corresponding capsule according to the protocol during the perioperative period. We compared the trends in perioperative hematologic findings and the postoperative outcomes. Patients' feces were collected for bacterial 16S rRNA sequencing. Patients were followed up at 1 month postoperatively. RESULTS: After the application of probiotics, the patients' postoperative inflammatory response level was reduced, and the trend of postoperative NLR decrease was significantly faster in the patients of the PG group than in the CG group (P = 0.047, partial η2 = 0.054). The trend of postoperative increase in serum albumin concentration in the patients of the PG group was significantly better than that in the CG group (P = 0.016, partial η2 = 0.078). In addition, patients in the PG group met discharge criteria earlier postoperatively and had fewer medical expenses. The quality of life of PG group was improved postoperatively. Postoperative inflammation-related markers, including the ratio of Firmicutes/Bacteroidetes, were increasing in untreated patients. In addition, the postoperative microbial diversity and abundance in the PG group remained stable. CONCLUSIONS: Probiotic compounds can reduce the inflammatory response after gastrectomy and enhance the recovery of the DGC patients by maintaining the stability of the gut microbiota.

Size Distributions and Health Risks of Particulate Polycyclic Aromatic Hydrocarbons in the Atmosphere at Coastal Areas in Ningbo, China.

Most current research focusing on the health risk assessments of particulate polycyclic aromatic hydrocarbons (PAHs) have not analyzed the size distributions and human respiratory deposition rates. In the present study, size-separated particulate matter (PM) was collected in the coastal area of Ningbo using an Anderson eight-stage air sampler over a 1-year period (2014-2015). The 16 US Environmental Protection Agency priority PAHs associated with PM were pretreated with rapid solvent extraction and analyzed by gas chromatography-mass spectrometry. The respiratory exposure assessment was determined using the multiple-path particle dosimetry (MPPD) model. The results show that all PAHs exhibited bimodal distribution with one mode peak in accumulation mode (0.43-0.65 µm) and another mode peak in coarse mode (4.7-5.8 µm). In addition, a low coefficient of divergence of PAHs between PM2.1 and PM2.1-10 indicated a high spatial heterogeneity in source factor contribution and formation mechanism. The deposition fluxes (tracheobronchial + pulmonary) of PM were highest for children in the size range of 3.3 µm < particle diameter (Dp) < 9 µm, while for males and females the highest fluxes occurred in the size range of 1.1 µm < Dp < 2.1 µm. The depositions of coarse PM in children were significantly higher than those in adults. The benzo[a]pyrene equivalent (BaPeq) depositions of dibenz[a,h]anthracene ranged from 1.4e-04 to 0.015 ng h-1, which were highest among the PAHs. The PAHs on particles with Dp >4.7 µm contributed approximately three times more to children than to males and females. Therefore, the toxicity of coarse PM to children needed attention. The incremental lifetime cancer risks (ILCR) for children, males, and females were estimated to be 2.92 × 10-7, 1.82 × 10-7, and 2.38 × 10-7, respectively, which were below the cancer risk guideline value (10-6). These ILCR values were much lower than the risks calculated without considering particle size distributions and respiratory depositions. The combination of the size-segregated sampling technique and the MPPD model can effectively avoid the overestimation of human respiratory exposure. Environ Toxicol Chem 2024;00:1-14. © 2024 SETAC.

Experimental Investigation of Reflectarray Antennas for High-Power Microwave Applications.

The power capacity of reflectarray antennas (RAs) is investigated through full-wave simulations and high-power microwave (HPM) experiments in this paper. In order to illustrate the results in detail, two RA elements are designed. The simulated power handling capacity of two RA elements are 7.17 MW/m2 and 2.3 GW/m2, respectively. To further study the HPM RA, two RA prototypes operating at 2.8 GHz are constructed with the aperture size of 1 m × 1 m. Simulations and experimental measurements are conducted for the two prototypes. The experimental results demonstrate that, even when subjected to 1 GW of power, the radiation beam of the RA with the second elements can still propagate in the intended direction. This research will establish a basis for advancing the practicality of RAs in HPM applications.

Recent Advances in Flexible Pressure Sensors Based on MXene Materials.

In the past decade, with the rapid development of wearable electronics, medical health monitoring, the Internet of Things, and flexible intelligent robots, flexible pressure sensors have received unprecedented attention. As a very important kind of electronic component for information transmission and collection, flexible pressure sensors have gained a wide application prospect in the fields of aerospace, biomedical and health monitoring, electronic skin, and human-machine interface. In recent years, MXene has attracted extensive attention because of its unique 2D layered structure, high conductivity, rich surface terminal groups, and hydrophilicity, which has brought a new breakthrough for flexible sensing. Thus, it has become a revolutionary pressure-sensitive material with great potential. In this work, the recent advances of MXene-based flexible pressure sensors are reviewed from the aspects of sensing type, sensing mechanism, material selection, structural design, preparation strategy, and sensing application. The methods and strategies to improve the performance of MXene-based flexible pressure sensors are analyzed in details. Finally, the opportunities and challenges faced by MXene-based flexible pressure sensors are discussed. This review will bring the research and development of MXene-based flexible sensors to a new high level, promoting the wider research exploitation and practical application of MXene materials in flexible pressure sensors.

Paclitaxel liposome (Lipusu) based chemotherapy combined with immunotherapy for advanced non-small cell lung cancer: a multicenter, retrospective real-world study.

BACKGROUND: Paclitaxel liposome (Lipusu) is known to be effective in non-small cell lung cancer (NSCLC) as first-line treatment. This study aimed to evaluate the effectiveness and safety of paclitaxel liposome based chemotherapy plus PD-1/PD-L1 inhibitor in patients with advanced NSCLC. METHODS: In this multicenter, retrospective, real-world study, patients with advanced NSCLC who were administered paclitaxel liposome based chemotherapy plus PD-1/PD-L1 inhibitor in three centers (Peking University People's Hospital as the lead center) in China between 2016 and 2022 were included. Progression-free survival (PFS), overall survival (OS), objective response rate, disease control rate, and adverse events (AEs) were evaluated. RESULTS: A total of 49 patients were included, with 33 (67.3%) receiving paclitaxel liposome based chemotherapy plus PD-1/PD-L1 inhibitor as first-line treatment. There were 34 patients (69.4%) diagnosed with squamous cell carcinoma and 15 (30.6%) with adenocarcinoma. The median follow-up was 20.5 (range: 3.1-41.1) months. The median PFS and OS of all patients were 9.7 months (95% confidence interval [CI], 7.0-12.4) and 30.5 months (95% CI, not evaluable-not evaluable), respectively. Patients with squamous cell carcinoma and adenocarcinoma had median PFS of 11 months (95%CI, 6.5-15.5) and 9.3 months (95%CI, 7.0-12.4), respectively. The median PFS was 9.9 months (95%CI, 7.1-12.7) in patients who received the combined regimen as first-line treatment. Treatment-related AEs of any grade were observed in 25 (51.0%) patients, and AEs of grade 3 or worse were observed in nine patients (18.4%). The most common treatment-related AEs were myelosuppression (14.3%) and fever (10.2%). CONCLUSIONS: Paclitaxel liposome based chemotherapy plus PD-1/PD-L1 inhibitor prolonged the PFS in advanced NSCLC with acceptable safety, which was worthy of clinical application.

Exploring the moderating role of digital finance in the two-way foreign direct investment and green technology innovation nexus: an empirical evidence from China.

Based on the provincial panel data of China from 2011 to 2020, this paper explores the relationship between two-way FDI and green technology innovation and examines the moderating role of digital finance in the impact of two-way FDI on green technology innovation. The results show that (1) two-way FDI can significantly enhance the level of green technology innovation. (2) Digital finance plays a moderating effect in the process of two-way FDI to enhance the level of green technology innovation. The conclusion is still robust by replacing the dependent variable, eliminating special samples and shrinking the tail. (3) The results of sub-dimensional analysis show that the three sub-dimensions indicators of the digital finance also have a positive moderating effect on the relationship between two-way FDI and green technology innovation. (4) The results of sub-regional regression show that the moderating effect in the central and western regions is greater than that in the eastern region. The results of the study can provide reference for governments to formulate policies about digital finance, which is conducive to achieve high-quality opening-up and realize green development.

Clinical performance of BALF droplet digital PCR for differential diagnosis of Pneumocystis jirovecii pneumonia and Pneumocystis jirovecii colonization.

BACKGROUND: Accurate differentiation between Pneumocystis jirovecii (Pj) infection and colonization is crucial for effective treatment. METHODS: From September 2016 to June 2022, 89 immunocompromised patients with unexplained lung infiltrates and clinical suspicion of Pj pneumonia were enrolled at Peking University People's Hospital. Bronchoalveolar lavage fluid (BALF) of these patients were detected by quantitative PCR (qPCR) and droplet digital PCR (ddPCR). RESULTS: The performance of ddPCR was superior to qPCR in detecting Pj infection. Area under the curve was 0.97 (95 %CI: 0.94-1) for ddPCR of the BALF in all patients. The optimal threshold value for discriminating Pj infection from colonization by ddPCR was 13.98 copies/test, with a sensitivity of 97.96 %, specificity of 85.71 %. No obvious correlation between ddPCR copy number and disease severity was observed. CONCLUSION: BALF ddPCR exhibits robust potential in detecting Pj and effectively discriminating colonization and infection.

A fucoidan plant drink reduces Helicobacter pylori load in the stomach: a real-world study.

Background: Helicobacter pylori (Hp) infection is highly prevalent globally and is predominantly managed by antibiotics. Recently, the anti-adhesive, antioxidant, antitoxin, immunomodulatory, anti-coagulant, and anti-infective activities of fucoidan, a polysaccharide extracted from brown seaweeds, have been widely studied, and the results showed promise. Fucoidan has the potential to be utilized in Hp eradication therapy. Our present clinical study was designed to evaluate the efficiency of Lewuyou®, a fucoidan plant drink (FPD) in eradicating Hp in humans. Methods: This multi-center, clinical study was conducted between October 2020 and July 2021. Hp infection was confirmed by urea breath test (UBT). A total of 122 patients with confirmed Hp infection were enrolled; after exclusion of incomplete data, 85 eligible patients (37 males and 48 females aged 20-81 years) were included in the final analysis. FPD (50 mL per vial) was orally administered twice daily for a 4-week cycle, and 41 patients completed an 8-week cycle. Results: No adverse event (AE) was reported in all 122 participants who had consumed FPD. The Hp eradication rate and clearance rate were 77.6% (66/85) and 20.0% (17/85), respectively, after 4 weeks of FPD consumption and 80.5% (33/41) and 26.8 (11/41) , respectively, after 8 weeks of consumption. Conclusions: The 4- and 8-week protocols of FPD consumption were safe and effective at reducing Hp load on the gastric mucosa, with Hp eradicated in the majority of participants.

Effects of a chitosan nanoparticles encapsulation on the properties of litchi polyphenols.

Litchi polyphenols have very specific biological activities. Nevertheless, the low and inconsistent oral bioavailability and instability hinder the further application of litchi polyphenols in food systems. This work prepared litchi polyphenols loaded chitosan nanoparticles (LP-CSNPs) by ionic gelation method to enhance the encapsulation on the properties of litchi polyphenols. The optimum conditions of formation via single factors and the Box-Behnken design were chitosan (CS) concentration 1.065 mg/mL, sodium tripolyphosphate (TPP) concentration 0.975 mg/mL, and the mass ratios of polyphenols and CS 1:1 with encapsulation efficiency (EE%) of 45.53%. LP-CSNPs presented the nanosized range of particle size (mean 170 nm), excellent polydispersity index (PDI) (0.156 ± 0.025), and zeta potential values (+ 35.44 ± 0.59). The in vitro release in simulated gastric fluid (pH 1.2) and intestinal fluid (pH 6.8) during 100 h was 58.34% and 81.68%, respectively. LP-CSNPs could effectively improve the storage stability and had great antibacterial activity compared with unencapsulated litchi polyphenols.

Classification and function of γδT cells and its research progress in anti-glioblastoma.

Human peripheral blood T lymphocytes are classified into alpha-beta T (αßΤ) cells and gamma-delta T (γδΤ) cells based on the difference in T cell receptors (TCRs). αßT cells are crucial for the acquired immune response, while Î³Î´Τ cells, though only a small subset, can recognize antigenic substances. These antigens do not need to be processed and presented and are not restricted by MHC. This distinguishes Î³Î´Τ cells from αßT cells and highlights their distinct role in innate immunity. Despite their small number, Î³Î´Τ cells hold significant significance in anti-tumor, anti-infection and immune regulation. Glioblastoma (GBM) represents one of the most prevalent malignant tumors within the central nervous system (CNS). Surgical resection alone proves to be an ineffective method for curing this type of cancer. Even with the combination of surgical resection, radiotherapy, and chemotherapy, the prognosis of some individuals with glioblastoma is still poor, and the recurrence rate is high. In this research, the classification, biological, and immunological functions of γδT cells and their research progress in anti-glioblastoma were reviewed.

T2T-YAO: A Telomere-to-telomere Assembled Diploid Reference Genome for Han Chinese.

Since its initial release in 2001, the human reference genome has undergone continuous improvement in quality, and the recently released telomere-to-telomere (T2T) version - T2T-CHM13 - reaches its highest level of continuity and accuracy after 20 years of effort by working on a simplified, nearly homozygous genome of a hydatidiform mole cell line. Here, to provide an authentic complete diploid human genome reference for the Han Chinese, the largest population in the world, we assembled the genome of a male Han Chinese individual, T2T-YAO, which includes T2T assemblies of all the 22 + X + M and 22 + Y chromosomes in both haploid. The quality of T2T-YAO is much better than all currently available diploid assemblies, and its haploid version, T2T-YAO-hp, generated by selecting the better assembly for each autosome, reaches the top quality of fewer than one error per 29.5 Mb, even higher than that of T2T-CHM13. Derived from an individual living in the aboriginal region of the Han population, T2T-YAO shows clear ancestry and potential genetic continuity from the ancient ancestors. Each haplotype of T2T-YAO possesses ∼ 330-Mb exclusive sequences, ∼ 3100 unique genes, and tens of thousands of nucleotide and structural variations as compared with CHM13, highlighting the necessity of a population-stratified reference genome. The construction of T2T-YAO, a truly accurate and authentic representative of the Chinese population, would enable precise delineation of genomic variations and advance our understandings in the hereditability of diseases and phenotypes, especially within the context of the unique variations of the Chinese population.

Atmospheric wet deposition of trace metal elements: Monitoring and modelling.

Trace elements (TEs), a group of atmospheric pollutants, have attracted considerable attention from scientists and government administrators worldwide. The wet deposition fluxes of nineteen trace elements (NTE) were monitored at Wanqingsha, a coastal site in the Pearl River Delta, for three consecutive years (2016.9-2019.8). Significant seasonal differences in NTE between wet and dry seasons were observed. The fluxes of crustal elements (Ca, Na, Al, Mg, K, Fe, Zn and Ba) were significantly higher than those of anthropogenic elements, accounting for over 99 % of the total annual wet deposition of 19 elements. Analysis of PM2.5 and rainfall samples reveals that both the fraction of each TE in the PM2.5 (CQ) and the Apparent Scavengance Ratio for TE (ASR, defined as the concentration ratio in rain and PM2.5) follow lognormal distributions. The logCQ variation for each element is relatively small but shows substantial differences, with means ranging from -5.48 to -2.03, while the logASRs for all elements show similar means (varying from 5.86 to 7.64) and an extremely wide range of variation. The influences of meteorological factors on CQ and ASR were also investigated. A simple box model framework was constructed to reasonably simplify the TE removal process by precipitation. The corresponding regression analysis showed significant correlations between NTE and the precipitation rate, PM2.5 concentration, ASR, and CQ, with R2 ranging from 0.711 to 0.970. By substituting the effects of environmental factors on ASR and CQ into the above relationship, temporal variations in NTE can be predicted. The reliability of the model was demonstrated by comparing model simulations with observations over three years. For most elements, the models can predict the temporal variation of NTE quite accurately, and even for the worst predictions, such as Al, Mg, K, Co and Cd, where predictions exceed observations by only an order of magnitude.

Study on the Treatment of Refined Sugar Wastewater by Electrodialysis Coupled with Upflow Anaerobic Sludge Blanket and Membrane Bioreactor.

In this paper, refined sugar wastewater (RSW) is treated by electrodialysis (ED) coupled with an upflow anaerobic sludge blanket (UASB) and membrane bioreactor (MBR). The salt in RSW was first removed by ED, and then the remaining organic components in RSW were degraded by a combined UASB and MBR system. In the batch operation of ED, the RSW was desalinated to a certain level (conductivity < 6 mS·cm-1) at different dilute to concentrated stream volume ratios (VD/VC). At the volume ratio of 5:1, the salt migration rate JR and COD migration rate JCOD were 283.9 g·h-1·m-2 and 13.84 g·h-1·m-2, respectively, and the separation factor α (defined as JCOD/JR) reached a minimum value of 0.0487. The ion exchange capacity (IEC) of ion exchange membranes (IEMs) after 5 months of usage showed a slight change from 2.3 mmol·g-1 to 1.8 mmol·g-1. After the ED treatment, the effluent from the tank of the dilute stream was introduced into the combined UASB-MBR system. In the stabilization stage, the average COD of UASB effluent was 2048 mg·L-1, and the effluent COD of MBR was maintained below 44-69 mg·L-1, which met the discharge standard of water contaminants for the sugar industry. The coupled method reported here provides a viable idea and an effective reference for treating RSW and other similar industrial wastewaters with high salinity and organic contents.

How Does Spatial Injustice Affect Residents' Policy Acceptance of the Economic-Social-Ecological Objectives of Construction Land Reduction?

Construction land reduction (CLR) is a policy innovation for Shanghai to explore high-quality economic development, but it will also lead to spatial injustice in the implementation process. Although the literature on spatial injustice and CLR is increasing, very little is known about the influence of spatial injustice in CLR on residents' policy acceptance of the economic-social-ecological objectives of CLR. To fill the knowledge gap, this study uses micro-survey data to identify the factors that influence residents' policy acceptance of the economic-social-ecological objectives of CLR. Results show that: (1) Spatial injustice in CLR significantly reduces residents' policy acceptance of the social and ecological objectives of CLR. (2) The locational disadvantage of villages significantly reduces residents' policy acceptance of the ecological objectives of CLR. (3) The more educated the residents are, the more they recognize the social and ecological objectives of CLR. (4) The higher the percentage of household workers, the more residents endorse the economic and social objectives of CLR. (5) Compared with ordinary residents, cadres are more accepting of the economic objectives of CLR. (6) Robustness tests support the findings of this study. The findings of this study provide insights for sustainable CLR policy reform.

Land Development Rights, Spatial Injustice, and the Economic Development in Net-Incremental Reduction Regions of Construction Land: Evidence from Shanghai, China.

Rapid urbanization raises the issue of protecting development interests in net-incremental reduction regions of construction land (NRRCL). Spatial injustice (SI) is one of the key factors for the smooth implementation of construction land reduction (CLR) policies. This study theoretically analyzes the influence of SI on the economic development in CLR saving quota outflow regions, namely, NRRCL, and conducted empirical tests with the difference-in-differences model. The findings reveal that: (1) regional differences in CLR policy promote the transfer of land development rights from NRRCL to net-incremental increase regions of construction land (NIRCL) in economically developed regions, thus resulting in SI; (2) SI limits the economic development of NRRCL; (3) land-use planning negatively impacts economic development in planning reduced-type regions; (4) the off-site realization of spatial justice in the CLR process in suburbs has comparative advantages; (5) in the process of CLR, it is vital to promote the transfer of population from NRRCL to NIRCL to alleviate the negative impact of SI.

Metabolic landscape dysregulation in bronchoalveolar lavage fluid of checkpoint inhibitor pneumonitis.

BACKGROUND: Checkpoint inhibitor pneumonitis (CIP) is a potentially fatal adverse event resulting from immunotherapy in patients with malignant tumors. However, the pathogenesis of CIP remains poorly understood. METHODS: We collected bronchoalveolar lavage fluid (BALF) from cohorts of patients with CIP, new-onset lung cancer (LC), and idiopathic pulmonary fibrosis (IPF). Non-targeted metabolomics analysis was conducted to analyze metabolic signatures. Flow cytometry was used to evaluate immune cell subsets. RESULTS: Lymphocytes were predominant in the BALF of patients with CIP. A total of 903 metabolites were identified, among which lipid compounds were the most abundant. In a comparison between patients with CIP and LC, enrichment analysis of the altered metabolites showed suppressed amino sugar metabolism, and spermidine and spermine biosynthesis in the CIP group. Metabolism of alpha linolenic acid, linoleic acid, and their fatty acid derivatives was enriched in the CIP group relative to the IPF group. The twelve metabolites found to be enriched in the CIP group were positively correlated with the proportion of CD8+ T cells. One cluster of BALF metabolites, 57.14% of which were lipid molecules, was inversely correlated with the proportion of natural killer cells. CONCLUSIONS: In this study, the metabolomic landscape of BALF in patients with CIP was determined. We elucidated suppressed tumor metabolic signatures, enhanced pulmonary inflammatory signaling, and the characteristics of responsible immune cells, which helps to understand the pathogenesis of CIP.

A retrospective case-control study of facial emotion recognition in male veterans with chronic schizophrenia and its correlation with interpersonal communication.

OBJECTIVE: To understand the facial emotion recognition of male veterans with chronic schizophrenia and the relationship between facial emotion recognition and interpersonal communication to provide a reference for designing social skills training programmes. METHOD: Fifty-six eligible male patients with chronic schizophrenia who were admitted to our hospital from October 2020 to April 2021 were selected, and 24 healthy people were selected as controls. Facial emotion recognition, social communication skills and self-perceived interpersonal disturbance were assessed using a facial emotion recognition stimulus manual, the Social Skills Checklist (SSC) and the Interpersonal Relationship Integrative Diagnostic Scale (IRIDS). Disease status was assessed using the Positive and Negative Syndrome Scale. RESULTS: Both the control group and the patient group had the highest recognition accuracy for neutral faces. The recognition rate for neutral expression was higher in the control group than in the patient group (p = 0.008). The rate of neutral expressions identified as happiness was higher in the patient group than in the control group (p = 0.001). The identification of anger as happiness was higher in the control group than in the patient group (p = 0.026), and the pattern of misidentification was similar between the control group and the patient group. The accuracy of facial emotion recognition was negatively associated with the age of onset (p < 0.05). The recognition accuracy for happiness was negatively associated with negative symptoms, general pathological symptoms and total scale scores (p < 0.05). The total score for expression recognition was negatively associated with the negative symptom subscale scores (p < 0.05), and there was no correlation between expression recognition and positive symptoms (p > 0.05). The recognition accuracy for happiness was negatively correlated with the IRIDS conversation factor (p < 0.05). The recognition accuracy for happiness and anger and the total scores for facial emotion recognition were negatively correlated with the SSC subscale score and the total score (p < 0.05 and p < 0.01, respectively). The main influencing factors on facial emotion recognition were the SSC total score (p < 0.001) and the positive factor score (p = 0.039). CONCLUSION: Veterans with chronic schizophrenia have facial emotion recognition impairments affected by negative symptoms. There is a correlation between facial emotion recognition and interpersonal communication. HIGHLIGHTS: 1. There are extensive facial expression recognition disorders in schizophrenia. 2. The pattern of misidentification was similar in both the control group and the patient group, with the tendency for happiness to be identified as a neutral emotion, anger as happiness, and fear as neutral emotion and anger. 3. Based on the comprehensive assessment of social cognitive impairment in patients with schizophrenia, prospective studies of standardised interventions are designed to provide support for clinical practice.

Influence of applied voltage on bioelectrochemical enhancement of biomethanation for low-rank coal and microbial community distribution.

The performance of peat biomethanation was investigated in bioelectrochemical anaerobic digestion at different applied voltages, and compared to conventional anaerobic digestion. The methane yield was stabilized at 16 mL/g peat in the conventional anaerobic digestion. However, in the bioelectrochemical anaerobic digestion, the methane yield was significantly increased to 264 mL/g peat at the applied voltage of 4 V, followed by 1 V, 2 V, 0.5 V and 0 V. The bioelectrochemical system could enrich more electroactive microorganisms on the electrode, as well as in the bulk solution, and further improve the direct interspecies electron transfer for methane production. The 16S rRNA analysis showed a significant increase in the abundance of specific microorganisms in the bulk solution, including Firmicutes phylum and Proteobacteria phylum, in addition to a gradual increase in acetoclastic methanogenesis with an increase in applied voltage. These results provide a solution to turn low-rank coal into a new alternative energy.

Noninvasive computed tomography derived fractional flow reserve simulation based on microvascular tree model reconstruction.

To establish a novel method for noninvasive computed tomography derived fractional flow reserve (CT-FFR) simulation based on microvascular tree model reconstruction and to evaluate the feasibility and diagnostic performance of the novel method in coronary artery disease compared with invasive fractional flow reserve (FFR). Twenty patients (20 vessels) who underwent coronary computed tomography angiography (CCTA) and invasive FFR were retrospectively studied. The anatomic epicardial coronary artery model was reconstructed based on CCTA image, and the microvascular tree model was simulated based on patient-specific anatomical structures and physiological principles. Numerical simulation was subsequently performed using the CFD method with full consideration of the variation of viscosity in microvascular. Two patients with the FFR value of .80 were selected for adjusting the parameters of the model, while the remaining 18 patients were selected as a validation cohort. After simulation, CT-FFR was compared with invasive FFR with a threshold of .80. Eleven (55%) patients had an abnormal FFR that was less than or equal to .80. Compared with invasive FFR, sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of CT-FFR with an optimal threshold of .80 were 100%, 77.8%, 81.8%, 100%, 88.89%, respectively. There were a good correlation and consistency between CT-FFR and invasive FFR. Time per patient of CT-FFR analysis was less than 15 min. CT-FFR based on microvascular tree model reconstruction is feasible with good diagnostic performance. It requires a short processing time with excellent accuracy. Large multicenter prospective studies are required for further demonstrating the diagnostic performance of this novel model in myocardium ischemia evaluation.