RESUMO
BACKGROUND: The motive of this study was to investigate the collaboration between MDR1 gene polymorphisms and anesthetic effects following pediatric tonsillectomy. METHODS: All together 178 children undergoing tonsillectomy with preoperative sevoflurane-remifentanil anesthesia were selected. In order to determine MDR1 gene polymorphisms of 3435Câ>âT, 1236Câ>âT, and 2677Gâ>âT/A, polymerase chain reaction-restriction fragment length polymorphism was used. Mean arterial pressure (MAP), diastolic blood pressure (DBP), systolic blood pressure (SBP), and heart rate (HR) at T0 (5 mins after the repose), T1 (0âmin after tracheal intubation), T2 (5 mins after the tracheal intubation), T3 (0âmin after the tonsillectomy), T4 (0âmin after removal of the mouth-gag) and T5 (5âmin after the extubation) were observed. The visual analog scale (VAS), the face, legs, activity, cry, and consolability (FLACC) pain assessment, and Ramsay sedation score were recorded after the patients gained consciousness. The adverse reactions were also observed. RESULTS: As compared to the CTâ+âTT genotype of MDR1 1236Câ>âT, the time of induction, respiration recovery, eye-opening, and extubation of children with the CC genotype was found to be shorter (all Pâ<.05); the MAP, SBP, DBP, and HR were significantly reduced at T5 in children that possessed the CC genotype (all Pâ<.05), the VAS at postoperative 1, 2, 4, and 8âhours and Ramsay sedation score were decreased, while the FLACC score increased (all Pâ<.05). It was found that the adverse reaction rate was lower in children bearing the CC genotype (Pâ<.05). CONCLUSION: It could be concluded that anesthetic effect in patients with the MDR1 1236Câ>âT CC genotype was found to be superior to those carrying the CTâ+âTT genotype.
Assuntos
Anestésicos/uso terapêutico , Éteres Metílicos/uso terapêutico , Variantes Farmacogenômicos , Piperidinas/uso terapêutico , Subfamília B de Transportador de Cassetes de Ligação de ATP/genética , Anestésicos/efeitos adversos , Pressão Sanguínea/efeitos dos fármacos , Pressão Sanguínea/genética , Criança , Pré-Escolar , Quimioterapia Combinada , Feminino , Técnicas de Genotipagem , Frequência Cardíaca/efeitos dos fármacos , Frequência Cardíaca/genética , Humanos , Hipnóticos e Sedativos/efeitos adversos , Hipnóticos e Sedativos/uso terapêutico , Masculino , Éteres Metílicos/efeitos adversos , Medição da Dor , Piperidinas/efeitos adversos , Polimorfismo de Nucleotídeo Único , Cuidados Pré-Operatórios , Remifentanil , Respiração/efeitos dos fármacos , Sevoflurano , TonsilectomiaRESUMO
In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0% ~ 5.7% and 2.6% ~ 7.3% respectively, while those between genera 5.6% ~ 22.5% and 7.3% ~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus. It was concluded that CYP86MF gene was not applicable to specie and subspecie taxon but genus taxon because the differences of sequences in nucleotides and amino acids were lower between species than genera.