[Open reduction and internal fixation in treatment of four cases of bipolar clavicle dislocations].

Objective: To summarize the method and effectiveness of open reduction and internal fixation in the treatment of 4 cases of bipolar clavicle dislocations. Methods: Between June 2017 and June 2022, 4 patients with bipolar clavicle dislocations were admitted. There were 3 males and 1 female. The age ranged from 27 to 63 years, with an average age of 45 years. There were 2 cases of crushing injury of mine car, 1 case of traffic accident injury, and 1 case of heavy object injury. The time from injury to operation was 3-7 days, with an average of 5.0 days. The sternoclavicular joint dislocations were classified as Grade Ⅱ in 1 case and type Ⅲ in 3 cases, and anterior dislocation in 3 cases and posterior dislocation in 1 case. The acromioclavicular joint dislocations were classified as Tossy type Ⅱ in 2 cases and type Ⅲ in 2 cases. After open reduction, the sternoclavicular joint and acromioclavicular joint were fixed with lateral malleolus locking titanium plate and clavicular hook plate, respectively. Results: All operations were successfully completed without vascular or nerve injury. All incisions healed by first intention. All patients were followed up 12-18 months, with an average of 14 months. At last follow-up, the shoulder joint functions were rated as excellent in 3 cases and good in 1 case according to Rockwood score. During follow-up, there was no loosening of internal fixator or fracture. The internal fixators were removed in all patients at 5-7 months after operation (mean, 6 months), and no re-dislocation occurred after removal. Conclusion: For bipolar clavicle dislocation, open reduction combined with lateral malleolus locking titanium plate fixation of the sternoclavicular joint and clavicle hook plate fixation of the acromioclavicular joint can achieve good effectiveness. It has the advantages of simple operation, high safety, firm fixation, and fewer complications, and the shoulder function recovers well.

[Highly expressed circular RNA hsa_circ_0017247 promotes osteosarcoma cell invasion and migration].

Objective To investigate the role of circular RNA hsa_circ_0017247 in osteosarcoma cell invasion and migration. Methods Real-time quantitative PCR (qRT-PCR) was used to detect the expression of hsa_circ_0017247 in the matched osteosarcoma and adjacent tissues, as well as in Saos-2 and hFOB1.19 cells. The correlation between its expression and the clinicopathological parameters of osteosarcoma was analyzed using Fisher exact probability test. After knockdown of hsa_circ_0017247 by siRNA in Saos-2 cells, qRT-PCR was conducted to detect the transfection efficiency. TranswellTM invasion and migration assays were performed to evaluate the changes in cell invasion and migration. The mRNA and protein levels of E-cadherin and N-cadherin in the Saos-2 cells were tested by qRT-PCR and Western blotting, respectively. Results Compared with the adjacent tissues, hsa_circ_0017247 was highly expressed in the osteosarcoma tissues. Osteosarcoma with high expression of hsa_circ_0017247 had a higher clinical stage and metastasis rate. Highly expressed hsa_circ_0017247 was found in the Saos-2 cells as compared with the hFOB1.19 cells. The hsa_circ_0017247 significantly decreased after siRNA transfection in the Saos-2 cells, and silencing hsa_circ_0017247 inhibited the invasion and migration of the Saos-2 cells. Moreover, silencing hsa_circ_0017247 also promoted the expression of E-cadherin, but inhibited the expression of N-cadherin. Conclusion High expression of hsa_circ_0017247 enhances osteosarcoma cell invasion and migration through accelerating epithelial mesenchymal transformation.