Discarded enoki mushroom root-derived multifunctional chrome-free chitosan-based tanning agent for eco-leathers manufacturing: Tanning-dyeing, non-acid soaking, and non-basifying.

The aim of this study was to develop new discarded enoki mushroom root-derived multifunctional chrome-free chitosan-based tanning agents that can be used for eco-leather manufacturing. In this study, oligochitosan (OCS) was prepared from chitosan extracted from the enoki mushrooms and chemically modified using reactive dye R19 and epichlorohydrin (ECH) to prepare chromium-free tanning agent (OCS-R19-ECH) with both tanning and dyeing functions. FT-IR, XRD, and NMR (1H) confirmed the successful synthesis of the product. The molecular weight of OCS-R19-ECH is 6355 g/mol, with an average particle size of 1249.37 nm and an epoxy value of 0.276 mol/100 g. OCS-R19-ECH was used for tanning experiments on bated sheepskin, and the results showed that the leather tanned with OCS-R19-ECH not only exhibited excellent wet-heat stability (shrinkage temperature = 81 °C), but also superior dyeing uniformity, resistance to dry and wet abrasion, mechanical strength (tensile strength = 12.4 MPa, tear strength = 57.3 N/mm), and outstanding antimicrobial properties. Most importantly, compared with traditional tanning agents, OCS-R19-ECH has a higher pH (9.0), tanning-dyeing integration, non-acid soaking, and non-basifying can be achieved in leather making, which can greatly simplify the tanning processes. This new multifunctional chrome-free chitosan-based tanning agent facilitates high-value utilization of waste resources.

Biocompatible and Biodegradable 3D Graphene/Collagen Fiber Hybrids for High-Performance Conductive Networks and Sensors.

Polymer-based flexible conductive materials are crucial for wearable electronics, electronic skin, and other smart materials. However, their development and commercial applications have been hampered by the lack of strain tolerance in the conductive network, poor bonding with polymers, discomfort during wear, and a lack of biocompatibility. This study utilized oil-tanned leather with a natural network structure, high toughness, and high tensile deformation recovery as a structural template. A graphene (Gr) conductive network was then constructed on the collagen network of the leather, with coordination cross-linking between Gr and collagen fibers through aluminum ions (Al3+). A new flexible conductive material (Al-GL) was then constructed. Molecular dynamics simulations and experimental validation revealed the existence of physical adsorption, hydrogen bonding adsorption, and ligand bonding between Al3+, Gr, and collagen fibers. Although we established that the binding sites between Al3+ and collagen fibers were primarily on carboxyl groups (-COOH), the mechanism of chemical bonding between Gr and collagen fibers remains unclear. The Al-GL composite exhibited a high shrinkage temperature (67.4 °C) and low electrical resistance (16.1 kΩ·sq-1), as well as good softness (9.33 mN), biocompatibility, biodegradability (<60 h), and air and moisture permeability. Furthermore, the incorporation of Al3+ resulted in a heightened Gr binding strength on Al-GL, and the resistance remained comparable following 1 h of water washing. The Al-GL sensor prepared by WPU encapsulation not only demonstrated highly sensitive responses to diverse motion signals of the human body but also retained a certain degree of response to external mechanical effects underwater. Additionally, the Al-GL-based triboelectric nanogenerator (Al-GL TENG) exhibited distinct response signals to different materials. The Al-GL prepared by the one-pot method proposed in this study offers a novel approach to combining functional nanofillers and substrate materials, providing a theoretical foundation for collagen fiber-based flexible conductive materials.

Reversible immobilization of cellulase on gelatin for efficient insoluble cellulose hydrolysis.

Immobilized enzymes are one of the most common tools used in enzyme engineering, as they can substantially reduce the cost of enzyme isolation and use. However, efficient catalysis of solid substrates using immobilized enzymes is challenging, hydrolysis of insoluble cellulose by immobilized cellulases is a typical example of this problem. In this study, inspired by bees and honeycombs, we prepared gelatin-modified cellulase (BEE) and gelatin hydrogels (HONEYCOMB) to achieve reversible recycling versus release of cellulase through temperature-responsive changes in the triple-stranded helix-like interactions between BEE and HONEYCOMB. At elevated temperatures, BEE was released from HONEYCOMB and participated in hydrolytic saccharification. After 24 h, the glucose yields of both the free enzyme and BEE reached the same level. When the temperature was decreased, BEE recombined with HONEYCOMB to facilitate the effective separation and recycling of BEE from the system. The enzymatic system retained >70 % activity after four reuse cycles. In addition, this system showed good biocompatibility and environmental safety. This method increases the mass transfer capacity and enables easy recovery of immobilized cellulase, thereby serving as a valuable strategy for the immobilization of other enzymes.

Extraction of chitin from flammulina velutipes waste: A low-concentration acid pretreatment and aspergillus Niger fermentation approach.

In recent years, with the booming of the edible mushroom industry, chitin production has become increasingly dependent on fungi and other non-traditional sources. Fungal chitin has advantages including superior performance, simpler separation processes, abundant raw materials, and the absence of shellfish allergens. As a kind of edible mushroom, flammulina velutipes (F. velutipes) also has the advantages of wide source and large annual yield. This provided the possibility for the extraction of chitin. Here, a procedure to extract chitin from F. velutipes waste be presented. This method comprises low-concentration acid pretreatment coupled with consolidated bioprocessing with Aspergillus niger. Characterization by SEM, FTIR, XRD, NMR, and TGA confirmed that the extracted chitin was ß-chitin. To achieve optimal fermentation of F. velutipes waste (80 g/L), ammonium sulfate and glucose were selected as nitrogen and carbon sources (5 g/L), with a fermentation time of 5 days. The extracted chitin could be further deacetylated and purified to obtain high-purity chitosan (99.2 % ± 1.07 %). This chitosan exhibited a wide degree of deacetylation (50.0 % ± 1.33 % - 92.1 % ± 0.97 %) and a molecular weight distribution of 92-192 kDa. Notably, the yield of chitosan extracted in this study was increased by 56.3 % ± 0.47 % compared to the traditional chemical extraction method.

Atomic-scale Ru anchored on chromium-shavings as a precursor for a pH-universal hydrogen evolution reaction electrocatalyst.

In the leather manufacturing industry, the management of substantial quantities of solid waste containing chrome shavings remains a formidable challenge. Concurrently, there is a pressing need for the development of pH-universal and economically viable electrocatalysts for the hydrogen evolution reaction (HER). In response to these intertwined challenges, this study proposes an innovative approach wherein the amino groups present on the surface of chrome shavings are utilized to immobilize single ruthenium atoms during pyrolysis, thereby facilitating the synthesis of hydrogen evolution electrocatalysts. The optimized sample, denoted as CN/Cr2O3/Ru-1, demonstrates exceptional electrocatalytic performance, exhibiting an ultra-low overpotential of -28 mV in 1.0 M KOH at a current density of 10 mA cm-2, and it also exhibits good performance in acidic and neutral electrolytes. Importantly, these overpotentials surpass those reported for many previous ruthenium-based catalysts. Density functional theory (DFT) calculations elucidate that both oxygen (O) and chromium (Cr) moieties within Cr2O3 can engage in favorable interactions with the coordination patterns of the ruthenium (Ru) atoms, thereby elucidating the synergistic enhancement conferred by the chromium element in CN/Cr2O3/Ru, which ultimately facilitates and promotes the catalytic activity of the ruthenium atoms serving as the catalytic center. This facile synthesis route not only presents a green solution for addressing waste chromium pollutants but also offers a promising avenue for the development of high-performance, cost-efficient electrocatalysts.

New plant polyphenol-derived tannic acid-based chromium-free tanning agent for sustainable and clean leather production.

This study aimed to prepare a new bio-based chromium-free tanning agent. The green epoxide monocase ethylene glycol diglycidyl ether (EGDE) was grafted with tannic acid (TA) derived from natural plant using the one-pot method to synthesize new plant polyphenol-derived tannic acid-based chromium-free tanning agents (TA-EGDE) with abundant terminal epoxides. FTIR, 1H NMR, XPS, GPC, SEM, and other analytical techniques were used to characterize tanning agents. These consequences manifested that EGDE was successfully grafted with the phenol hydroxyl group of TA. The epoxide value of TA-EGDE showed a tendency to increase and then decrease with increasing EGDE dosage, and the epoxide value of TA-EGDE-2 attained a maximum of 0.262 mol/100 g. GPC analysis showed that the formula weight of the prepared TA-EGDE was partially distributed above 5000 Da. The tanning experiment demonstrated that the shrinkage temperatures (Ts) of the TA-EGDE-tanned leathers were all higher than 81.5 °C. Compared with the traditional commercial chromium-free tanning agent (F-90, TWS), TA-EGDE-tanned leathers exhibited higher Ts and better mechanical properties. The TA-EGDE prepared in this study not only has ecological environmental protection but also provides finished leather with good moisture, heat resistance, and mechanical properties.

Biomimetic Exogenous "Tissue Batteries" as Artificial Power Sources for Implantable Bioelectronic Devices Manufacturing.

Implantable bioelectronic devices (IBDs) have gained attention for their capacity to conformably detect physiological and pathological signals and further provide internal therapy. However, traditional power sources integrated into these IBDs possess intricate limitations such as bulkiness, rigidity, and biotoxicity. Recently, artificial "tissue batteries" (ATBs) have diffusely developed as artificial power sources for IBDs manufacturing, enabling comprehensive biological-activity monitoring, diagnosis, and therapy. ATBs are on-demand and designed to accommodate the soft and confining curved placement space of organisms, minimizing interface discrepancies, and providing ample power for clinical applications. This review presents the near-term advancements in ATBs, with a focus on their miniaturization, flexibility, biodegradability, and power density. Furthermore, it delves into material-screening, structural-design, and energy density across three distinct categories of TBs, distinguished by power supply strategies. These types encompass innovative energy storage devices (chemical batteries and supercapacitors), power conversion devices that harness power from human-body (biofuel cells, thermoelectric nanogenerators, bio-potential devices, piezoelectric harvesters, and triboelectric devices), and energy transfer devices that receive and utilize external energy (radiofrequency-ultrasound energy harvesters, ultrasound-induced energy harvesters, and photovoltaic devices). Ultimately, future challenges and prospects emphasize ATBs with the indispensability of bio-safety, flexibility, and high-volume energy density as crucial components in long-term implantable bioelectronic devices.

Engineering eco-friendly and biodegradable biomass-based multifunctional antibacterial packaging films for sustainable food preservation.

The study presents a new class of eco-friendly and biodegradable biomass-based multifunctional antibacterial packaging films (G-OCSI) based on oxidized corn starch-based nonionic biopolymer (OCSI) and gelatin (Gel), and investigates the effects of different OCSI contents on the properties of G-OCSI. The results demonstrated that G-OCSI 0.25 had good water vapor barrier properties, antioxidant activity (DPPH RSA: 85.84 %), UV resistance (UV blocking > 99.9 %), water resistance (WCA: 122.30°), and tensile properties. Based on the disk diffusion experiment, G-OCSI exhibited significant bactericidal and antibacterial effects against S. aureus and E. coli. Moreover, G-OCSI had good biodegradability in natural environments, and could obviously accelerate the crops growth. Finally, a banana preservation experiment confirmed that G-OCSI could significantly extend the shelf life of bananas at room temperature at least 3 days. The biodegradable packaging films not only realizes the sustainable utilization of biomass resources but also has the potential to replace traditional petroleum-based plastics.

A temperature and pressure dual-responsive, stretchable, healable, adhesive, and biocompatible carboxymethyl cellulose-based conductive hydrogels for flexible wearable strain sensor.

The study aimed to develop a novel temperature and pressure dual-responsive conductive hydrogel with self-healing, self-adhesive, biocompatible, and stretchable properties, for the development of multifunctional anti-counterfeiting and wearable flexible electronic materials. A conductive hydrogel based on carboxymethyl cellulose (CMC) was synthesized by simple "one pot" free radical polymerization of CMC, acrylamide (AAm) and acrylic acid (AAc). The hydrogel displayed temperature responsiveness and possessed an upper critical solution temperature (UCST) value. In addition, hydrogels also had surprising pressure responsiveness. The synthesized hydrogels were characterized by FTIR, TGA, DSC, and XRD analysis. Importantly, the obtained hydrogels exhibited exceptional mechanical properties (stress: 730 kPa, strain: 880%), fatigue resistance, stretchability, self-healing capability, self-adhesive properties, and conductivity. In addition, valuable insights were obtained into the synthesis and application of flexible anti-counterfeiting and camouflage materials by the temperature and pressure dual-responsive hydrogels. Moreover, the prepared hydrogel, with an electrically sensitive perception of external strain (GF = 2.61, response time: 80 ms), can be utilized for monitoring human movement, emotional changes, physiological signals, language, and more, rendering it suitable for novel flexible anti-counterfeiting materials and versatile wearable iontronics. Overall, this study provided novel insights into the simple and efficient synthesis and sustainable manufacturing of environmentally friendly multifunctional anti-counterfeiting materials and flexible electronic skin sensors.

Development of an activatable Lysosome-targeted fluorescent probe for the detection of endogenous ONOO- levels.

The liver plays a crucial role in several important processes in the human body, including metabolism, detoxification, and immune function. When the liver experiences acute injury, it can cause significant harm and requires prompt detection. Traditional biomarkers lack specificity and cannot detect changes in real-time, making them unsuitable for monitoring pathological processes. Recent studies have shown that acute liver injury (ALI) is closely related to oxidative stress, with peroxynitrite (ONOO-) being a vital byproduct of liver metabolism and become a critical biomarker for detecting liver damage. As a result, this research developed an activatable near-infrared fluorescent probe W-3a that can be used to detect endogenous ONOO- in a mouse model of ALI induced by lipopolysaccharides (LPS). The probe has high selectivity and anti-interference ability, with a reaction time <10 min and a detection limit of 85 nM. It was successfully utilized in detecting endogenous ONOO- in cells and live imaging of ALI mice.

Eco-Friendly Cellulose-Based Nonionic Antimicrobial Polymers with Excellent Biocompatibility, Nonleachability, and Polymer Miscibility.

This study aims to prepare natural biomass-based nonionic antimicrobial polymers with excellent biocompatibility, nonleachability, antimicrobial activity, and polymer miscibility. Two new cellulose-based nonionic antimicrobial polymers (MIPA and MICA) containing many terminal indole groups were synthesized using a sustainable one-pot method. The structures and properties of the nonionic antimicrobial polymers were characterized using nuclear magnetic resonance hydrogen spectroscopy (1H NMR), infrared spectroscopy (FTIR), wide-angle X-ray diffractometry (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), gel chromatography (GPC), and other analytical techniques. The results showed that microcrystalline cellulose (MCC) molecules combined with indole derivatives through an esterification reaction to produce MICA and MIPA. The crystallinity of the prepared MICA and MIPA molecules decreased after MCC modification; their morphological structure changed from short fibrous to granular and showed better thermal stability and solubility. The paper diffusion method showed that both nonionic polymers had good bactericidal effects against the two common pathogenic bacteria Escherichia coli (E. coli, inhibition zone diameters >22 mm) and Staphylococcus aureus (S. aureus, inhibition zone diameters >38 mm). Moreover, MICA and MIPA showed good miscibility with biodegradable poly(vinyl alcohol) (PVA), and the miscible cellulose-based composite films (PVA-MICA and PVA-MIPA) showed good phase compatibility, light transmission, thermal stability (maximum thermal decomposition temperature >300 °C), biocompatibility, biological cell activity (no cytotoxicity), nonleachability, antimicrobial activity, and mechanical properties (maximum fracture elongation at >390%).

Engineering Self-Adaptive Multi-Response Thermochromic Hydrogel for Energy-Saving Smart Windows and Wearable Temperature-Sensing.

Buildings account for ≈40% of the total energy consumption. In addition, it is challenging to control the indoor temperature in extreme weather. Therefore, energy-saving smart windows with light regulation have gained increasing attention. However, most emerging base materials for smart windows have disadvantages, including low transparency at low temperatures, ultra-high phase transition temperature, and scarce applications. Herein, a self-adaptive multi-response thermochromic hydrogel (PHC-Gel) with dual temperature and pH response is engineered through "one-pot" integration tactics. The PHC-Gel exhibits excellent mechanical, adhesion, and electrical conductivity properties. Notably, the low critical solubility temperature (LCST) of PHC-Gel can be regulated over a wide temperature range (20-35 °C). The outdoor practical testing reveals that PHC-Gel has excellent light transmittance at low temperatures and radiation cooling performances at high temperatures, indicating that PHC-Gel can be used for developing energy-saving windows. Actually, PHC-Gel-based thermochromic windows show remarkable visible light transparency (Tlum ≈ 95.2%) and solar modulation (△Tsol ≈ 57.2%). Interestingly, PHC-Gel has superior electrical conductivity, suggesting that PHC-Gel can be utilized to fabricate wearable signal-response and temperature sensors. In summary, PHC-Gel has broad application prospects in energy-saving smart windows, smart wearable sensors, temperature monitors, infant temperature detection, and thermal management.

High-value utilization of Cr-containing sludge: Eco-friendly and ultra-low-cost electrocatalyst for efficient OER in alkaline media from Cr-containing sludge.

Economically sustainable development requires more viable waste recycling solutions. In this context, we address the problem of utilizing chromium-containing sludge, a prevalent and environmentally hazardous waste. Meanwhile, sustainable energy development must develop ecology-friendly and low-cost electrocatalysts for the oxygen evolution reaction (OER) in alkaline media. Herein, we report an ultra-low-cost electrocatalyst from chromium-containing sludge. The optimum preparation conditions are determined by optimizing the calcination temperature and the loading of nickel acetylacetonate. The optimized catalyst delivers excellent stability and outstanding OER activity with overpotentials of 320 mV at 10 mA cm-2 in alkaline media. Density functional theory calculations reveal that the energy barrier of OER is decreased because of the catalyst's heterogeneous structure arrangement and confirm the influence of chromium on performance improvement. The concept of "turning waste into treasure" stimulates the search for methods to process Cr-containing waste and produce low-cost, high-performance electrocatalysts.

Chemoselective Fluorogenic Bioconjugation of Vicinal Dithiol-Containing Proteins for Live Cellular Imaging via Small Molecular Conjugate Acceptors.

To develop small molecular fluorogenic tools for the chemoselective labeling of vicinal dithiol-containing proteins (VDPs) in live cells is important for studying intracellular redox homeostasis. With this research, we developed small molecule-based fluorescent probes, achieving selective labeling of VDPs through thiol-thiol substitutions on bisvinylogous thioester conjugated acceptors (IDAs). Initially, IDAs demonstrated its ability to bridge vicinal cysteine-sulfhydryls on a peptide as a mimic. Then, the peptide complex could be decoupled to recover the original peptide-SH in the presence of dithiothreitol. Furthermore, fluorometric signal amplification of the fluorescent probes occurred with high sensitivity, low limit of detection, and selectivity toward vicinal dithiols on reduced bovine serum albumin, as an example of real world VDPs. More importantly, the probes were utilized successfully for labeling of endogenous VDPs at different redox states in live cells. Thus, the bisvinylogous thioester-based receptor as a functional probe represents a new platform for uncovering the function of VDPs in live cells.

Self-assembled nano-particles of chitosan amphiphilic derivative for formaldehyde fluorescent detection and its application in test strips.

Excessive levels of formaldehyde (FA) represent serious health risks. Aiming at the detection of formaldehyde content, this paper proposes a self-assembly method of proportional nanoprobes. Spherical nanoparticles (NPs) were prepared by one-step condensation reaction between rhodamine B (RhB) and chitosan (CS). After CS was modified by RhB, the linear structure changed and self-assembled under the action of "hydrophilic/hydrophobic" to form a core-shell structure with a cavity structure. The hydrophobic small molecule probe N-Butyl-4-Hydrazo-1,8-Naphacticimide (NBHN) spontaneously entered into the hydrophobic cavity to form spherical particles Chitosan-Rhodamine B@N-Butyl-4-Hydrazo-1,8-Naphacticimide (CS-RhB@NBHN) with a size of about 60 nm. The hydroxyl groups on CS enrich formaldehyde through charge interaction, and promote the reaction of formaldehyde with NBHN, so that the probe can detect formaldehyde at a lower concentration (detection limit 87 nmol·L-1). The self-assembled CS-RhB@NBHN nanoparticles significantly increased the response speed of NBHN (from 30 min to 10 min). After the reaction of NBHN with formaldehyde, the PET effect is released, the fluorescence transition from red to yellow of CS-RhB@NBHN, and the visual fluorescence response effect to formaldehyde is significantly improved. With the help of smartphone color recognition software, we converted the color of the probe solution into RGB values to realize the quantitative and visual detection of formaldehyde. In addition, CS-RhB@NBHN was used for the detection of FA in leather and air.

Intelligent detection strategy and bioimaging application of dual-responsive Hg2+ and ONOO- using near-infrared probes.

Mercury is a highly toxic heavy metal pollutant. Mercury and its derivatives pose serious threats to the environment and the health of organisms. Numerous reports have indicated that Hg2+ exposure induces a burst of oxidative stress in organisms, causing severe damage to the health of the organism. A large number of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are produced under conditions of oxidative stress, and superoxide anions (O2-) and NO radicals react rapidly with each other to produce peroxynitrite (ONOO-), an important downstream product. Therefore, developing an efficient and highly responsive screening method to monitor the fluctuations of Hg2+ and ONOO- levels is particularly important. In this work, we designed and synthesized a highly sensitive and highly specific near-infrared probe W-2a, which can effectively detect and distinguish Hg2+ and ONOO- through fluorescence imaging. In addition, we developed a WeChat mini-program called "Colorimetric acquisition" and built an intelligent detection platform to assess the environmental hazards of Hg2+ and ONOO-. The probe can detect Hg2+ and ONOO- in the body through dual signaling, as evidenced by cell imaging, and has successfully monitored fluctuations in the ONOO- levels in inflamed mice. In conclusion, the W-2a probe provides a highly efficient and reliable method for assessing oxidative stress-induced changes in the ONOO- levels in the body.

New indoleacetic acid-functionalized soluble oxidized starch-based nonionic biopolymers as natural antibacterial materials.

This study aims to develop a new soluble oxidized starch-based nonionic antibacterial polymer (OCSI) featuring high antibacterial activity and non-leachability by grafting indoleacetic acid monomer (IAA) onto the oxidized corn starch (OCS). The synthesized OCSI was characterized analytically by Nuclear magnetic resonance H-spectrometer (1H NMR), Fourier transform infrared spectroscopy (FTIR), Ultraviolet-visible spectroscopy (UV-Vis), X-ray diffractometer (XRD), X-ray Photoelectron Spectroscopy (XPS), Scanning Electronic Microscopy (SEM), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). The results showed that the synthesized OCSI was endowed with high thermal stability and favorable solubility, and the substitution degree reached 0.6. Besides, the disk diffusion test revealed a lowest OCSI inhibitory concentration of 5 µg disk-1, and showed significant bactericidal activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli). Moreover, the antibacterial films (OCSI-PCL), featuring their good compatibility, mechanical properties, antibacterial activity, non-leachability, and low water vapor permeability (WVP), were also successfully prepared by blending OCSI with biodegradable polycaprolactone (PCL). Finally, CCK-8 assay results confirmed the excellent biocompatibility of the OCSI-PCL films. Overall, this very study evidenced the applicability of the obtained oxidized starch-based biopolymers as an eco-friendly non-ionic antibacterial material and confirmed their promising applications in areas including biomedical materials, medical devices, and food packaging.

Discovery of C-5 Pyrazole-Substituted Pyrrolopyridine Derivatives as Potent and Selective Inhibitors for Janus Kinase 1.

Developing selective inhibitors for Janus kinase 1 (JAK1) is a significant focus for improving the efficacy and alleviating the adverse effects in treating immune-inflammatory diseases. Herein, we report the discovery of a series of C-5 pyrazole-modified pyrrolopyrimidine derivatives as JAK1-selective inhibitors. The potential hydrogen bond between the pyrazole group and E966 in JAK1 is the key point that enhances JAK1 selectivity. These compounds exhibit 10- to 20-fold JAK1 selectivity over JAK2 in enzyme assays. Compound 12b also exhibits excellent JAK1 selectivity in Ba/F3-TEL-JAK cellular assays. Metabolism studies and the results of the hair growth model in mice indicate that compound 12b may be a viable lead compound for the development of highly JAK1-selective inhibitors for immune and inflammatory diseases.

Hypochlorous acid-activated near-infrared fluorescent probe for in vivo/exogenous detection and dairy toxicity evaluation.

Oxidative stress has been reported to be closely associated with many diseases, and an excessive overdose of hypochlorite (ClO-) can also induce stress-related diseases. In this study, we designed and synthesized a NIR probe, named W-1a based on computational analysis of DCM (4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran) derivatives for specific detection of ClO-. The probe exhibited dual fluorescence and colorimetric sensing with a response time of <1 min and a detection limit of 0.15 µM. Additionally, the probe was successfully applied for fluorescence imaging of ClO- at the cellular level and ebrafish endogenous/exogenous ClO- assay and dairy toxicity assessment. Thus, we present a potential method for developing an efficient and reliable detection of ClO- in early stage using near-infrared dyes.

Nature-Skin-Derived e-Skin as Versatile "Wound Therapy-Health Monitoring" Bioelectronic Skin-Scaffolds: Skin to Bio-e-Skin.

Electronic skins (e-skins) have the potential to turn into breakthroughs in biomedical applications. Herein, a novel acellular dermal matrix (ADM)-based bioelectronic skin (e-ADM) is used to fabricate versatile "wound therapy-health monitoring" tissue-nanoengineered skin scaffolds via a facile "one-pot" bio-compositing strategy to incorporate the conductive carbon nanotubes and self-assembled micro-copper oxide microspheres with a cicada-wing-like rough surface and nanocone microstructure. The e-ADM exhibits robust tensile strength (22 MPa), flexibility, biodegradability, electroactivity, and antibacterial properties. Interestingly, e-ADM exhibits the pH-responsive ability for intelligent command between sterilization and wound repair . Additionally, e-ADM enables accurate real-time monitoring of human activities, providing a novel flexible e-skin sensor to record injury and motions. In vitro and in vivo experiments show that with electrical stimulation, e-ADM could prominently facilitate cell growth and proliferation and further promote full-thickness skin wound healing, providing a comprehensive therapeutic strategy for smart sensing and tissue repair, guiding the development of high-performance "wound therapy-health monitoring" bioelectronic skin-scaffolds.