RESUMO
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
Assuntos
Técnicas Genéticas , Plantas Geneticamente Modificadas , Zea mays/genética , Aclimatação , Agricultura/instrumentação , Agricultura/métodos , Agrobacterium tumefaciens/genética , Vetores Genéticos , Sementes/genética , Sementes/crescimento & desenvolvimento , Transformação Genética , Zea mays/crescimento & desenvolvimentoRESUMO
In this chapter we describe an Agrobacterium tumefaciens transformation method of soybean that utilizes mature half seeds and regeneration from the cotyledonary node region. This method results in fertile transformed soybean plants and transgenic seed in approximately 9 months. Using mature half seeds as starting material has proven to be a reliable method that does not require additional wounding for infection to occur. We have continued to make improvements in the protocol, resulting in an efficient plant regeneration system.
