Effect of bismuth subcarbonate against Campylobacter pylori: do citrate ions improve antibacterial activity?

The minimum inhibitory concentrations of three bismuth subcarbonate preparations: 1. bismuth subcarbonate, 2. a mixture of 50% bismuth subcarbonate and 50% citric acid, 3. a mixture of 50% bismuth subcarbonate and 50% potassium citrate were assayed against 30 strains of Campylobacter pylori employing the agar dilution method. Bismuth subcarbonate alone exhibited an MIC50 of 2 mg/l and was more effective than the two combinations (MIC50 4 mg/l). It is concluded that the addition of citrate ions does not improve antibacterial activity.

The minimum inhibitory concentrations of various bismuth salts against Campylobacter pylori.

The minimum inhibitory concentrations of five bismuth salts (bismuth subcitrate, bismuth subgallate, bismuth subnitrate, bismuth subsalicylate and tripotassium dicitrato bismuthate, a water soluble compound of bismuth subcitrate) were assayed against 48 strains of Campylobacter pylori employing the agar dilution method. Tripotassium dicitrato bismuthate was most effective (MIC50 8 mg/l), the other bismuth salts exhibited somewhat lower inhibitory activities. It is concluded that bismuth salts are suitable agents for inhibiting growth of Campylobacter pylori.

In search of an animal model for experimental Campylobacter pylori infection: administration of Campylobacter pylori to rodents.

Various mouse strains and Lewis rats were either orally or intravenously infected with high inocula of Campylobacter pylori and bacterial counts in the liver, spleen, heart, kidneys, and the stomach were followed over time. The intravenously infected animals displayed transient bacteremia and colonization of their livers and spleens. A loglinear decrease led to complete bacterial clearance after 72 hours. Oral administration of Campylobacter pylori neither resulted in colonization nor tissue invasion in any of the infected mice, even if infection had occurred under complete drug-induced achlorohydria. We conclude that under the conditions chosen, mice and rats are not suited for an animal model for experimental Campylobacter pylori infection.

[Campylobacter pylori in the stomach, duodenum and colon of gastroenterological patients. An epidemiologic study of 120 subjects]. / Campylobacter pylori in Magen, Duodenum und Kolon gastroenterologischer Patienten. Eine epidemiologische Studie an 120 Personen.

In a prospective study of 120 gastroenterological patients in Berlin, Germany, the prevalence of Campylobacter pylori was determined. When the gastric mucosa was normal, the prevalence was one in 19 patients (5.3%). In 101 patients with chronic gastritis it was cultured in 55 (54.5%). In 31 patients with chronic atrophic gastritis the organism was cultured in 25 (81%); in 60 patients with severe gastritis it was present in 75%, in 35 with moderate or severe chronic active gastritis in 82.8%. The diagnosis of gastric ulcer (9) or duodenal ulcer (12) was associated with the isolation of Campylobacter pylori in 55.6 and 91.7%, respectively. The prevalence of this organism in antral mucosa of this group of patients thus corresponds to that in Australia, England and North America. The organism was also demonstrated in the duodenum of 10 among 25 patients examined. But in none of 25 patients was it demonstrated in the sigmoid colon.

Comparative investigations about three different identification systems for Campylobacter pyloridis from human antrum mucosa.

Campylobacter pyloridis (C. p.) which was first isolated in 1983 in Australia by Marshall and Warren from human gastric mucosa has since been associated with chronic gastritis and ulceration of stomach and duodenum. A typical characteristic of this microorganism is very strong urease formation. Therefore, from the appearance of that enzyme in the gastric mucosa colonization with C. p. can be inferred. The investigations reported here were done to compare the cultivation of C. p. as a direct proof of the presence of this microorganism with two different enzymatic procedures to be used as a rapid indirect method for demonstration of the presence of C. p. A total of 100 patients were involved. The results show rather good agreement between all three procedures used (80%), so that, at least for epidemiological surveys, the demonstration of urease may be used as an indirect procedure for the demonstration of the presence of C. p.