RESUMO
An enzyme-linked immunosorbent assay for antibodies to varicella-zoster virus (VZV), using purified viral glycoproteins as antigen (gpELISA), was compared with other assays for measuring vaccine-induced antibody responses. The gpELISA was more sensitive than conventional assays, proved highly specific for VZV and agreed well with an assay for neutralizing antibody activity. It was successfully applied to large-scale testing of live varicella vaccine in humans.
Assuntos
Anticorpos Antivirais/análise , Ensaio de Imunoadsorção Enzimática/métodos , Herpesvirus Humano 3/imunologia , Vacinas Virais/imunologia , Anticorpos Antivirais/biossíntese , Antígenos Virais , Vacina contra Varicela , Estudos de Avaliação como Assunto , Imunofluorescência , Glicoproteínas/imunologia , Humanos , Testes de Neutralização , Proteínas Virais/imunologiaRESUMO
The development of an ELISA of increased sensitivity has permitted a more critical evaluation of human humoral immune responses to the live attenuated varicella (Oka/Merck) vaccine. For use as a solid-phase antigen, the glycoprotein (gp) antigens are prepared by lectin-affinity chromatography from lysates of VZV-infected MRC-5 cells. The lot-to-lot variation in VZV gp content is controlled by standardization of antigen against a panel of human serum providing antigen-coated plates of consistent quality. The increased sensitivity of the gpELISA over the VAR ELISA is reflected in the greater seroconversion rate and prepositive rate specificity. These determinations have been shown to be specific for anti-VZV by absorption experiments using purified VZV gp antigens.
Assuntos
Anticorpos Antivirais/sangue , Antígenos Virais/imunologia , Ensaio de Imunoadsorção Enzimática/normas , Glicoproteínas/imunologia , Herpesvirus Humano 3/imunologia , Criança , Pré-Escolar , Cromatografia de Afinidade , Humanos , Lactente , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Controle de Qualidade , Reprodutibilidade dos TestesRESUMO
A modified CF-32 Beckman flow centrifuge rotor has been developed that provides a long sedimentation path length with high gravitational force at the gradient sample interface. The modified rotor exhibits excellent separative capability and extraction efficiency when applied to purification of human influenza B and herpes simplex viruses.