Assuntos
Infecções por HIV/tratamento farmacológico , Inibidores da Protease de HIV/efeitos adversos , Indinavir/efeitos adversos , Nefropatias/induzido quimicamente , Cristalização , Infecções por HIV/urina , Inibidores da Protease de HIV/química , Inibidores da Protease de HIV/urina , Humanos , Indinavir/química , Indinavir/urinaRESUMO
Indinavir is a potent HIV-1 protease inhibitor included in current antiretroviral therapeutic regimens. It is associated with renal and urological complications ascribed to indinavir crystalluria. We have previously reported that indinavir crystalluria is frequently observed soon after initiation of therapy. In a cohort of 54 asymptomatic indinavir-naive HIV-1-infected individuals during their first year of treatment with indinavir, approximately 25% of urinalyses (U/A) contained indinavir crystals. Because the determinants of the crystalluria are unknown, we examined the relationship between urine specific gravity (SG) and pH, singly and in combination, and indinavir crystalluria in these subjects. A total of 579 U/A were obtained from the study subjects at their scheduled monthly outpatient medical assessments. The frequency of indinavir crystalluria was lower in U/A with lower pH, irrespective of the SG. Conversely, U/A with high pH (> or = 6.0) had a higher frequency of indinavir crystalluria, which was further influenced by the urine SG. As a result, nearly half of the U/A (46.7%) with high pH (> or = 6.0) and intermediate-high SG (> or = 1.015) contained indinavir crystals. In conclusion, the frequency of indinavir crystalluria in asymptomatic HIV-1 infected individuals during their first year of treatment with indinavir was markedly influenced by the urine pH and SG. Our findings suggest that low urine pH may have a protective effect against indinavir crystalluria across the entire range of urine SG.
Assuntos
Fármacos Anti-HIV/uso terapêutico , Fármacos Anti-HIV/urina , Infecções por HIV/tratamento farmacológico , Infecções por HIV/urina , Indinavir/uso terapêutico , Indinavir/urina , Adulto , Idoso , Fármacos Anti-HIV/efeitos adversos , Cristalização , Feminino , Humanos , Concentração de Íons de Hidrogênio , Indinavir/efeitos adversos , Masculino , Pessoa de Meia-Idade , Gravidade Específica , Urinálise , UrinaRESUMO
There has been interest in the literature in the possible existence of a gene that predisposes to both breast cancer (BC) and colorectal cancer (CRC). We describe the detailed characterisation of one kindred, MON1080, with 10 cases of BC or CRC invasive cancer among 26 first-, second- or third-degree relatives. Linkage analysis suggested that a mutation was present in BRCA2. DNA sequencing from III: 22 (diagnosed with lobular BC) identified a BRCA2 exon 3 542G>T (L105X) mutation. Her sister (III: 25) had BC and endometrial cancer and carries the same mutation. Following immunohistochemical and microsatellite instability studies, mutation analysis by protein truncation test, cDNA sequencing and quantitative real-time PCR revealed a deletion of MSH2 exon 8 in III: 25, confirming her as a double heterozygote for truncating mutations in both BRCA2 and MSH2. The exon 8 deletion was identified as a 14.9 kb deletion occurring between two Alu sequences. The breakpoint lies within a sequence of 45 bp that is identical in both Alu sequences. In this large BC/CRC kindred, MON1080, disease-causing truncating mutations are present in both MSH2 and BRCA2. There appeared to be no increased susceptibility to the development of colorectal tumours in BRCA2 mutation carriers or to the development of breast tumours in MSH2 mutation carriers. Additionally, two double heterozygotes did not appear to have a different phenotype than would be expected from the presence of a mutation in each gene alone.
Assuntos
Neoplasias da Mama/genética , Neoplasias Colorretais/genética , Proteínas de Ligação a DNA , Genes BRCA2 , Mutação em Linhagem Germinativa , Proteínas/genética , Proteínas Proto-Oncogênicas , Adulto , Idoso , Sequência de Bases , Análise Mutacional de DNA , Reparo do DNA , DNA Complementar , Feminino , Ligação Genética , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Proteína 2 Homóloga a MutS , Neoplasias Primárias Múltiplas , Linhagem , Reação em Cadeia da PolimeraseRESUMO
Indinavir is a potent protease inhibitor widely used in combination with reverse-transcriptase inhibitors to treat human immunodeficiency virus (HIV) disease. Individuals treated with indinavir are prone to develop urinary complications, including renal colic, renal calculi, lower urinary tract symptoms, and indinavir crystalluria. Although renal stones secondary to indinavir have been described and characterized, little is known about the onset, frequency, and significance of the crystalluria. To document the longitudinal characteristics of indinavir crystalluria and associated urine abnormalities, 54 asymptomatic indinavir-naive HIV-positive individuals had urinalysis testing initially weekly and then monthly during the first year of indinavir treatment. Six hundred eight urinalyses were performed (11 +/- 2 urinalysis/subject), including 579 microscopy examinations performed by a nephrologist (10 +/- 2 examinations/subject). Baseline urinalysis results were essentially normal. After the start of treatment, indinavir crystalluria was frequently observed (67% of subjects). After the first 2 weeks, indinavir crystalluria remained constant at a frequency of approximately 25% of urine sediments examined at each test point. Other urine abnormalities, principally leukocytes (>/=10/high-power field) and casts, were observed in 39% of subjects. These abnormalities were more severe in five subjects, with concomitant increasing serum creatinine levels in three of them. Additional urine findings include the predominance of low pH (=5. 5 in 72% of urinalyses) and high specific gravity (>/=1.025 in 66% of urinalyses). In conclusion, abnormal urinalysis results were noted frequently during the first year of treatment with indinavir. The main findings were the high proportion of subjects with crystalluria and the relatively high frequency of crystalluria observed consistently throughout. These findings may occasionally be associated with other urine abnormalities, presumably secondary to indinavir crystalluria.
Assuntos
Inibidores da Protease de HIV/efeitos adversos , Soropositividade para HIV/tratamento farmacológico , Soropositividade para HIV/urina , Indinavir/efeitos adversos , Adulto , Idoso , Estudos de Coortes , Cristalização , Feminino , Inibidores da Protease de HIV/química , Inibidores da Protease de HIV/urina , Humanos , Concentração de Íons de Hidrogênio , Indinavir/química , Indinavir/urina , Contagem de Leucócitos , Masculino , Pessoa de Meia-Idade , Nefelometria e Turbidimetria , Estudos Prospectivos , Gravidade Específica , UrináliseRESUMO
Urinary complications observed during indinavir treatment of HIV disease are often attributed to indinavir crystalluria. In a prospective study of urinalysis during the first year of indinavir therapy, 5 of 54 asymptomatic HIV+ individuals presented severe leukocyturia (> or =100 cells/HPF) usually accompanying indinavir crystalluria. The clinical course of these 5 individuals, successfully treated for HIV and monitored for an second follow-up year, suggests that recurrence of severe leukocyturia may be an indicator of renal damage, likely tubulointerstitial disease caused by indinavir crystalluria. This is in contrast to the remaining 49 subjects, including those presenting mild leukocyturia, who did not demonstrate any evidence of renal disease. Regular urinalysis is therefore recommended in the clinical management of indinavir-treated individuals to detect early renal damage secondary to indinavir crystalluria and to prevent further renal impairment.
Assuntos
Inibidores da Protease de HIV/efeitos adversos , Soropositividade para HIV/tratamento farmacológico , Soropositividade para HIV/urina , HIV-1/imunologia , Indinavir/efeitos adversos , Leucócitos/efeitos dos fármacos , Leucocitose/induzido quimicamente , Urina/citologia , Adulto , Cristalização , Quimioterapia Combinada , Feminino , Inibidores da Protease de HIV/administração & dosagem , Humanos , Indinavir/administração & dosagem , Leucocitose/urina , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Análise de Regressão , Fatores de TempoAssuntos
Cartilagem Cricoide/diagnóstico por imagem , Cartilagem Cricoide/patologia , Neoplasias de Cabeça e Pescoço/diagnóstico por imagem , Neoplasias de Cabeça e Pescoço/patologia , Mieloma Múltiplo/diagnóstico por imagem , Mieloma Múltiplo/patologia , Idoso , Neoplasias de Cabeça e Pescoço/radioterapia , Humanos , Imageamento por Ressonância Magnética , Masculino , Mieloma Múltiplo/radioterapia , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVE: To determine whether complete resection of small-bowel metastases from melanoma improves patient survival. DESIGN: A computer-aided chart review. SETTING: Hospitals associated with McGill University. PATIENTS: Twenty patients (17 men, 3 women), identified from 1524 patients with melanoma, who underwent surgery to the small bowel for metastases. Patient age and clinical presentation, tumour site and stage were recorded. INTERVENTION: Exploratory laparotomy with complete or partial resection of involved small bowel. MAIN OUTCOME MEASURES: Operative morbidity, mortality and length of survival related to the extent of small-bowel resection. RESULTS: Eleven patients had complete resection, 8 patients had partial resection and 1 patient had a palliative bypass only. Long-term survival (ranging from 2 to 10 years) was 36% in those who had complete resection and 0% in those who had partial resection; operative morbidity and mortality were 20% and 15% respectively. CONCLUSION: Complete resection of small-bowel metastases in patients with metastatic melanoma can result in long-term survival.
Assuntos
Neoplasias do Íleo/cirurgia , Neoplasias do Jejuno/cirurgia , Melanoma/cirurgia , Neoplasias Cutâneas/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Mortalidade Hospitalar , Humanos , Neoplasias do Íleo/mortalidade , Neoplasias do Íleo/secundário , Neoplasias do Jejuno/mortalidade , Neoplasias do Jejuno/secundário , Masculino , Melanoma/mortalidade , Melanoma/secundário , Pessoa de Meia-Idade , Estudos Retrospectivos , Análise de SobrevidaRESUMO
The association between sebaceous neoplasms of the skin and visceral cancers, known as Muir-Torre syndrome, is described in three patients, including one with an extensive history of cancer in his family. The first patient, a 54-year-old man, developed multiple sebaceous adenomas, epitheliomas, and carcinomas in association with a colonic carcinoma 6 years after cardiac transplantation. Family history in this patient disclosed colon cancer in 17 relatives. The second patient was a 51-year-old man who had recurrent adenocarcinoma of the sigmoid colon, adenocarcinoma arising in Barrett's esophagus, and sebaceous epithelioma during a period of 15 years. The third patient was a 90-year-old man with a sebaceous adenoma followed 5 months later by adenocarcinoma of the sigmoid colon with liver metastases. Muir-Torre syndrome in 129 other patients published in the literature is reviewed. Although it is a rare disease, Muir-Torre syndrome requires recognition because skin lesions may be the first sign of the syndrome and this may lead to early diagnosis of associated visceral cancers. Moreover, because this syndrome appears to be inherited, family members should be screened for visceral cancer, especially colorectal adenocarcinoma.
Assuntos
Adenocarcinoma Sebáceo/patologia , Neoplasias Gastrointestinais/patologia , Neoplasias Cutâneas/patologia , Adenocarcinoma Sebáceo/genética , Adenocarcinoma Sebáceo/imunologia , Idoso , Idoso de 80 Anos ou mais , Neoplasias Gastrointestinais/genética , Neoplasias Gastrointestinais/imunologia , Humanos , Hospedeiro Imunocomprometido , Masculino , Pessoa de Meia-Idade , Linhagem , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/imunologia , SíndromeRESUMO
OBJECTIVE: To evaluate the prognostic importance for mortality of age, fever, hypertension, and involvement of renal, cardiac, neural, pulmonary, gastrointestinal and cutaneous systems as well as elevated transaminases, thrombocytosis, leukocytosis, anemia, smoking status, comorbid diseases and disease severity, in 45 patients with systemic necrotizing vasculitis (SNV) identified by histological, radiological, and clinical criteria. METHODS: Kaplan-Meier nonparametric survival functions and Cox proportional hazards regression models were used. RESULTS: Twenty-four deaths were observed during the 5 year mean followup period. Five year survival was 58%. Comorbidity and severity of involvement were not predictive of mortality. Multivariable survival analysis showed that cardiac or renal involvement was associated with a relative risk of dying of 2.91 (95% CI: 1.25, 6.77). Elevated serum transaminase demonstrated a trend to having a protective effect [relative risk of 0.14 (95% CI: 0.02, 1.07)]. No other variable was an independent predictor of fatality. No cohort effect could be documented following the introduction of cytotoxic drugs in the treatment of SNV. CONCLUSION: SNV remains associated with a high mortality due largely to renal or cardiac involvement.
Assuntos
Síndrome de Churg-Strauss/mortalidade , Poliarterite Nodosa/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Síndrome de Churg-Strauss/sangue , Síndrome de Churg-Strauss/urina , Estudos de Coortes , Comorbidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Poliarterite Nodosa/sangue , Poliarterite Nodosa/urina , Prognóstico , Estudos Retrospectivos , Análise de SobrevidaRESUMO
This study examines the utility of objective histopathological studies in the evaluation of adult patients with erythroderma. A series of 56 skin biopsies, from 40 erythrodermic patients, was reviewed sequentially by 4 Canadian dermatopathologists who were unaware of clinical details of the cases. The final diagnosis (gold standard), in each instance, had already been determined by others, based on clinicopathologic data and response to therapy. Direct comparison revealed that the mean accuracy of the histopathological diagnoses was 53% (range: 48-66%), a favorable result in view of the difficulty of the task at hand. Additional points of information which evolved from the study are as follows: (i) identification, by microscopy alone, of spongiotic dermatitis, cutaneous T-cell lymphoma and psoriasis, as underlying causes of erythroderma was more successful than that of drug eruptions and pityriasis rubra pilaris; (ii) the epidermotropism which characterizes cutaneous T-cell lymphoma may be mistaken for inflammatory interface changes seen in drug eruptions and vice versa, thus constituting a pitfall in diagnosis; (iii) finally, it appears that submission of multiple simultaneous biopsies, rather than a single specimen, from patients with erythroderma would be likely to enhance the accuracy of histopathological diagnosis.
Assuntos
Dermatite Esfoliativa/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Erros de Diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Pele/patologiaAssuntos
Xantogranuloma Juvenil/patologia , Idoso , Crioterapia , Seguimentos , Humanos , Masculino , Recidiva , Xantogranuloma Juvenil/terapiaRESUMO
In 1971, Costello described a new syndrome in 2 patients. The major clinical findings comprise short stature; redundant skin of the neck, palms, soles, and fingers; curly hair; relative macrocephaly; depressed nasal bridge; papillomata around the mouth and nares; distinct facial gestalt; hyperextensible joints; and mental retardation. We present a third patient and review the manifestations of this condition.
Assuntos
Anormalidades Múltiplas/patologia , Deformidades Congênitas do Pé , Deformidades Congênitas da Mão , Deficiência Intelectual , Face/anormalidades , Humanos , Recém-Nascido , Masculino , Papiloma , SíndromeRESUMO
We report an unusual case occurring in a 25-year-old male of a balloon cell nevus which also showed clinical and pathological features of a halo nevus.
Assuntos
Nevo Pigmentado/patologia , Neoplasias Cutâneas/patologia , Adulto , Humanos , Masculino , Melaninas/análise , Nevo Pigmentado/análise , Nevo Pigmentado/diagnóstico , Nevo Pigmentado/cirurgia , Neoplasias Cutâneas/análise , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/cirurgiaRESUMO
The association of vasculitis and asymptomatic primary biliary cirrhosis (PBC) was documented in a 39 year old female. Cyclophosphamide was used with good results. The Raji cell assay for circulating immune complexes (CIC) was positive initially with further values changing in parallel with disease activity. The C1q binding assay failed to reveal significant levels of CIC at any time. Lymphocytotoxic antibodies were not detected in the serum. Sucrose density ultra-centrifugation experiments revealed 9-17S material in sera that were positive for CIC by the Raji cell assay. We conclude that intermediate size CIC may be important in the pathogenesis of the vasculitis associated with PBC.