Designing a Massive Open Online Course (MOOC) in face-to-face sessions. A blended design to teach practical histology.

Teaching histology, through virtual microscopy in educational strategies, undeniably moved towards the digitization and distancing of teaching. The setting up of the Massive Open Online Course (MOOC) entitled "Introduction to Histology: exploring the tissues of the human body" made it possible to exploit the potential to share digital resources with a wider audience while being integrated into the teaching on-campus students. This article described the pedagogical choices prevailing during the design of the MOOC and its combination with face-to-face sessions to achieve specific learning outcomes. The pedagogical alignment of learning outcomes described according to their cognitive levels, with online and face-to-face learning activities and evaluation methods has been demonstrated. The impact of such a blended design into an academic program has been ascertained using perception and performance data. Student satisfaction and engagement as well as motivational cues were identified. The level of performance was maintained in the educational strategy implemented and made it possible to achieve the objectives expected by the teachers. The benefits of integrating a MOOC with classroom-based teaching were highlighted, as well as barriers that could hinder the successful implementation.

Assessment of the cutaneous immune response during Arthroderma benhamiae and A. vanbreuseghemii infection using an experimental mouse model.

BACKGROUND: Dermatophytoses are common but poorly understood skin infections. Most in vivo studies have been performed using the guinea pig as the experimental animal model, which has several limitations. OBJECTIVES: To develop a mouse model of dermatophytosis suitable for multiple purposes, including the investigation of immunity against dermatophytes. MATERIALS AND METHODS: Two peculiar fungal species, Arthroderma benhamiae and A. vanbreuseghemii, isolated from tinea in humans having contact with rodents were used for epicutaneous inoculation. During the infection, clinical and histopathological follow-up were performed. The recruitment of immune cells was evaluated by immunofluorescence staining and the levels of cytokine mRNA were quantified by quantitative reverse transcriptase-polymerase chain reaction in the skin of infected mice. RESULTS: The skin symptoms and microscopic lesions, including the colonization of keratinized epidermal and follicular structures by both dermatophytes, were highly similar to those observed in guinea pig infection models and in natural infections, mimicking acute superficial tinea in humans. The dermal inflammatory cellular infiltrate consisted of macrophages, dendritic cells and especially polymorphonuclear neutrophils, which are one of the histological 'clues' to the diagnosis of dermatophytosis. The in situ cytokine profile was characterized by the overexpression of transforming growth factor-ß, interleukin (IL)-1ß and IL-6 mRNA during infection, suggesting a role of the T-helper 17 pathway in the establishment of immunity. CONCLUSIONS: Our new reproducible and validated mouse model of dermatophytosis is a modern in vivo tool that allows a more in-depth understanding of the pathogenesis of human dermatophyte infections.

A family study of homeland Korean alcohol use.

Survey data were obtained from 199 homeland Korean families consisting of 199 sets of parents, and 300 college-age sons (162) and daughters (138). Data were obtained regarding quantity and frequency of alcohol consumption by users and of judgments of what constituted "normal" and "problem" alcohol use, flushing status, symptoms experienced following alcohol use, reasons for drinking given by users, and reasons for abstaining given by abstainers. Fathers and sons differed little in use status, but daughters were abstainers far less often than their mothers. Female users drank a good deal less than males, fathers and sons differed slightly, while daughters drank considerably more than their mothers. Own use was substantially correlated with judgments of the nature of normal use and far less related judgements of problem use. There was a significant difference in fast versus slow skin flushing. with a higher proportion of females being fast flushers. Fast flushers drank less than slow flushers and also judged lower amounts of consumption as being "normal." Fast flushers experienced more physical symptoms than slow flushers; persons who drank more experienced fewer symptoms. Males more than females, slow flushers more than fast flushers, and heavy more than light users of alcohol are more likely to endorse a variety of reasons for drinking. Reasons for not drinking differ little across family membership groups. Contemporary homeland Koreans drink less than would have been expected on the basis of prior research, even though there is a substantial generational difference among females, with daughters being less frequently abstainers and drinking more, and more often than their mothers.

Religious affiliation, religious involvement, and alcohol use in Korea.

This article is the first study of religious commitment and involvement of homeland Koreans as related to alcohol use. Religious affiliation and involvement are predictive of abstemiousness, but chiefly for women, who are at low risk for alcohol abuse and dependency, and not for men, who are at high risk. The frequent abstemiousness of Buddhist women results chiefly from data having to do with mothers and probably is a consequence of the women's traditional lifestyles, being both Buddhist and abstemious. Among the probably more Westernized Christians, religious involvement is most clearly associated with abstemiousness among daughters, a group at a relatively low but increasing risk for alcohol use.

Alcohol consumption and use norms among Chinese Americans and Korean Americans.

Homeland (Taiwan) Chinese are very low in alcohol consumption, while homeland Koreans are very high in alcohol consumption. Neither Chinese Americans nor Korean Americans constitute a large portion of the residents of Hawaii, but across four surveys, data were obtained from 212 Chinese-American and 72 Korean-American college students or students' parents. Persons from these groups do not differ greatly. Korean Americans are slightly higher in reported alcohol use and in judgments of the nature of normal alcohol use. The two groups do not differ in their judgments of the nature of problem alcohol use.

"Paradoxical" effects of morphine on antipredator defense reactions in wild and laboratory rats.

In a Fear/Defense Test Battery, measuring defensive reactions to a present, approaching and contacting predator, the highest dose of morphine tested (7.5 mg/kg) reliably reduced vocalization to dorsal contact, to vibrissae stimulation, and to an anesthetized conspecific in laboratory-bred wild R. norvegicus. Except for a dose-dependent reduction in flinch/jump reactions to dorsal contact (taps), other defensive behaviors (flight, freezing, etc.) were not reliably altered by morphine treatment (0, 1.0, 2.5, 7.5 mg/kg). Vocalization responses to vibrissae stimulation in wild-trapped R. rattus were reliably increased following naloxone (1.0 and 10.0 mg/kg) administration, lending support for opiate receptor involvement in the mediation of defensive vocalization. In the Anxiety/Defense Test Battery, measuring defensive reactions to situations associated with a predator (cat) or with cat odor, laboratory rats showed no decrease in defensive behavior with morphine (0, 1.0, 5.0 mg/kg). In direct contrast to the above findings, the effects of morphine treatment in this test battery suggested a generalized increase in defensiveness to noncontacting and nonpainful threat stimuli. These effects included a decrease in time spent near the cat compartment, with a complementary increase in time spent at maximum distance, a decrease in transits between these sections, an increase in crouching, and a decrease in grooming and rearing. This pattern of results suggests that morphine may have two opposing effects on defensive behavior, a generalized enhancement, together with a more specific reduction of responses to tactile or painful stimulation. A very widespread pattern of reliable sex or sex x drug effects in the Anxiety/Defense Test Battery was in good agreement with previous reports of sex differences in these tests, with females generally more defensive than males. Consonant with previous findings, no reliable sex differences were found with the Fear/Defense Test Battery, although several values approached an acceptable level of statistical significance.