Tris-Azo Triangular Paraphenylenes: Synthesis and Reversible Interconversion into Radial π-Conjugated Macrocycles.

We report the synthesis of cycloparaphenylene derivatives featuring tris-azo groups. The smaller derivative, [3]cycloazobenzene, adopts a triangular all-cis form and exhibits thermally and photochemically stable characteristics due to significant ring strain as well as symmetric Kagome-patterned crystal packing. In contrast, the as-synthesized [3]cycloazobenzene with three biphenylene bridges adopts a triangular all-cis form, which undergoes photoinduced isomerization, leading to a photostationary state. Interestingly, the addition of an excess of acid selectively leads to the formation of an all-trans form. DFT calculations reveal that the interconversion from a triangular to a circular shape correlates with an increase in HOMO and a decrease in LUMO, characteristics intrinsic to radial π-conjugated systems.

Longitudinal Associations Between Serum Bilirubin Level and Carotid Atherosclerosis Plaque in a Health Screening Population.

This study aimed to determine the relationship between bilirubin levels and carotid atherosclerosis (CAS) in the health screening population. After propensity score matching, this retrospective cohort study included 4360 subjects who underwent health examinations regularly in Hebei General Hospital between January 2010 and December 2019 and had no carotid plaque at baseline. After an average follow-up of 26.76 months, the main endpoint Cox regression analysis of carotid plaques was performed. After adjusting the confounding factors, Cox regression analysis showed that when serum total bilirubin (TBIL) and unconjugated bilirubin (UCB) increased by 1 standard deviation (SD), the risk of carotid plaque decreased by 7.30% (95% confidence interval (CI): 2.80-11.60%) and 15.70% (95% CI: 11.40-19.80%), respectively. When conjugated bilirubin (CB) increased by 1 SD, the risk of carotid plaques increased by 24.3% (95% CI: 19.7-29.0%). TBIL and UCB levels were negatively associated with CAS, and CB levels were positively associated with CAS.

Construction of heterojunction photoanode via facile synthesis of CoOx/CN nanocomposites for enhanced visible-light-driven photoelectrochemical degradation of clofibric acid.

Visible-light-driven photoelectrocatalytic (PEC) oxidation has been explored extensively to develop highly active materials. Herein, a visible-light-active p-Co3O4 and n-g-C3N4 heterojunction (CoOx/CN) photoanode, constructed by simple one-pot calcination, was shown to remove clofibric acid (CA) from water through a PEC process. Compared with pristine g-C3N4, the optimal photoanode (15%-CoOx/CN) exhibited stable and effective PEC performance and CA degradation performance, a 100-fold enhancement in photocurrent density, and around 1.5-fold decreased efficiency over 6 h. The p-n heterojunctions were shown to increased the charge density and conductivity of g-C3N4 for rapid charge transfer. Furthermore, interface contact broadened the visible light absorption and accelerated charge carrier transfer. Notably, the catalysts established p-n heterojunctions, which hindered the bulk recombination of photoinduced carriers and improved the charge separation efficiency. The CoOx/CN photoanodes showed a pair of redox peaks at a potential of 0.3 V vs. Ag/AgCl, indicating good Co3O4 redox behavior under alkaline conditions. The 15%-CoOx/CN photoanode displayed excellent PEC performance of up to 0.16 mA cm-2 in 0.1 M KOH solution at 1.23 V vs. RHE (reversible hydrogen electrode) and long-term stability for up to 12 h. The CoOx/CN photoanodes maintained excellent PEC activities for CA removal, even under acidic and alkaline conditions conditions (pH 3-10). Probable degradation pathway of CA was proposed according to the main degradation intermediates. This study shows that the synergistic effect of p-n heterojunctions in photoelectrodes provides a new approach to the rational application of new photoanode candidates and PEC performance optimization.

Accumulated Clinical Experiences from Successful Treatment of 1377 Severe and Critically Ill COVID-19 Cases.

In late December 2019, COVID-19 was firstly recognized in Wuhan, China and spread rapidly to all of the provinces of China. The West Campus of Wuhan Union Hospital, the designated hospital to admit and treat the severe and critically ill COVID-19 cases, has treated a large number of such patients with great success and obtained lots of valuable experiences based on the Chinese guideline (V7.0). To standardize and share the treatment procedures of severe and critically ill cases, Wuhan Union Hospital has established a working group and formulated an operational recommendation, including the monitoring, early warning indicators, and several treatment principles for severe and critically ill cases. The treatment experiences may provide some constructive suggestions for treating the severe and critically ill COVID-19 cases all over the world.

Numerical assessments of recharge-dominated groundwater flow and transport in the nearshore reclamation area in western Taiwan.

This study employed experimental and numerical methods to assess the behavior of conservative solute transport for a selected temporary solid waste site in a reclamation area in western Taiwan. Calibrating a site-specific numerical model, finite element model of water flow through saturated-unsaturated media (FEMWATER), relies on observations from field- and laboratory-scale hydraulic tests and spatial-temporal monitoring. The field-scale experiment used a modified hydraulic tomography survey (MHTS) to identify near surface aquifer stratifications and estimate the distribution of saturated hydraulic conductivity. The pressure plate experiments provided parameters for the van Genuchten soil characteristic model. Sensitivity analyses were then conducted based on varied recharge rates and dispersivities applied to the calibrated model. Observations of groundwater levels and salinity in the wells indicated that the regional groundwater flow was from southeast to northwest. In addition, a shallow freshwater layer was noted in the study area. The tidal-induced amplitudes for water level fluctuation in the wells ranged from 2 to 20 cm, depending on their distance from the seawater body. MHTS showed clear stratification, similar to that of well loggings at the storage site. The hydraulic conductivity at the test site ranged from 8 to 10 m/day, which is close to that obtained from the laboratory falling head test. The results of particle-tracking modeling showed that the critical recharge rate for the site needed to enhance plume traveling is 1000 mm/year. The increase in dispersivity values induced a decrease in plume travel time of up to 1000 days from the site to the coastal line. A special case for pulse releasing solute at the site shows that the key factor in controlling plume migration is the recharge rate. This is due to the low natural head gradient in the reclamation area. The results therefore suggest that a land drainage system near the site can play an important role in contaminant transport in the reclamation area.

Correlation between antiplatelet therapy in secondary prevention of acute cerebral infarction and cerebral microbleeds: A susceptibility-weighted imaging (SWI) study.

OBJECTIVE: To investigate the clinical significance of antiplatelet aggregation therapy for patients diagnosed with acute cerebral infarction (ACI) complicated with the cerebral microbleeds (CMBs). METHODS: Thirty patients with ACI and 36 patients with intracerebral hemorrhage (ICH) were included in this research. Two groups, studied by susceptibility-weighted imaging (SWI), were compared in terms of the number, location, and severity of CMBs. Then, 30 cases of ACI patients were divided into CMBs sub-group and non-CMBs sub-group. Univariate analysis between these two sub-groups was performed to determine the risk factors regarding the incidence of CMBs. For ACI patients, the number of CMBs before and after applying anti-platelet treatment were compared to examine the impacts of anti-platelet treatment on hemorrhagic transformation. RESULTS: CMBs were found to be more prevalent and severe in ICH patients than in ACI patients. CMBs in patients with ICH were more severe than in patients with ischemic stroke (IS), which indicates that CMBs closely relate to ICH. Hypertension and leukoaraiosis were found to have significant effects on the incidence of CMBs. After anti-platelet treatment, patients with CMBs (≥5) increased the number of CMB, whereas there was no obvious effect on patients with the CMBs less than 5 or no CMBs. CONCLUSIONS: The number of CMBs increased significantly among ACI patients with 5 or more CMBs before the anti-platelet treatment. CMBs are more frequently found in patients with hemorrhagic stroke than in patients with ischemic stroke, and more severe than the latter, which suggests that the clinical impact of higher association between the increase of the number of the CMBs and the hemorrhagic stroke.

Carbon Monoxide and Its Controlled Release: Therapeutic Application, Detection, and Development of Carbon Monoxide Releasing Molecules (CORMs).

Carbon monoxide (CO) is attracting increasing attention because of its role as a gasotransmitter with cytoprotective and homeostatic properties. Carbon monoxide releasing molecules (CORMs) are spatially and temporally controlled CO releasers that exhibit superior and more effective pharmaceutical traits than gaseous CO because of their chemistry and structure. Experimental and preclinical research in animal models has shown the therapeutic potential of inhaled CO and CORMs, and the biological effects of CO and CORMs have also been observed in preclinical trials via the genetic modulation of heme oxygenase-1 (HO-1). In this review, we describe the pharmaceutical use of CO and CORMs, methods of detecting CO release, and developments in CORM design and synthesis. Many valuable clinical CORMs formulated using macromolecules and nanomaterials are also described.

Effects of hydrogeological properties on sea-derived benzene transport in unconfined coastal aquifers.

This paper presents numerical investigations on quantifying the hydrodynamic effects of coastal environment factors, including tidal fluctuations, beach slopes, hydraulic conductivity, and hydraulic gradients on sea-derived benzene transport in unconfined coastal aquifers. A hydrologic transport and mixed geochemical kinetic/equilibrium reactions in saturated-unsaturated media model was used to simulate the spatial and temporal behaviors of the density flow and benzene transport for various hydrogeological conditions. Simulation results indicated that the tidal fluctuations lead to upper saline plumes (USPs) near the groundwater and seawater interfaces. Such local circulation zones trapped the seaward benzene plumes and carried them down in aquifers to the depth depending on the tide amplitudes and beach slopes across the coastal lines. Comparisons based on different tidal fluctuations, beach slopes, hydraulic conductivity, and hydraulic gradient were systematically conducted and quantified. The results indicated that areas with USPs increased with the tidal amplitude and decreased with the increasing beach slope. However, the variation of hydraulic conductivity and hydraulic gradient has relatively small influence on the patterns of flow fields in the study. The increase of the USP depths was linearly correlated with the increase of the tidal amplitudes. The benzene reactive transport simulations revealed that the plume migrations are mainly controlled by the local flow dynamics and constrained in the USP circulation zones. The self-cleaning process of a coastal aquifer is time-consuming, typically requiring double the time of the contamination process that the benzene plume reach the bottom of a USP circulation zone. The presented systematic analysis can provide useful information for rapidly evaluating seaward contaminants along a coastal line with available hydrogeological properties.

Quality of control of compound chlorhexidine acetate ointment / 药学实践杂志

Objective To improve the standard of the quality control of compound chlorhexidine acetate ointment . Methods TLC was used to control the quality of menthol crystal and camphor .A method to determine chlorhexidine acetate and cocaine hydrochloride simultaneously by HPLC was established .Results The spots of menthol crystal and camphor in TLC were clear .Chlorhexidine acetate and cocaine hydrochloride showed excellent linearity ,which were at the range of 10.01-160.14 μg/ml and 10 .01-160 .14 μg/ml ,respectively .The average recoveries were 101 .5% (RSD=1 .8% ) and 100 .5% (RSD=2 .8% ) .Conclusion The methods were simple ,sensitive and with good reproducibility and could be used to control the quality of compound chlorhexidine acetate ointment .

[Remote sensing analysis of forest resources characteristics in main ecological restoration counties in the Three-gorge area based on the 2nd-class inventory data].

Based on high-resolution SPOT-5 images, combined with topographic (1:10000) calculating terrain-bit index, the distribution characteristics of woodlands in different terrain niches, and the buffer radii of different roads, water bodies and settlements were identified by using ArcGIS space overlay and buffer analysis function. Results showed that woodland resources were abundant, and arbor woodland and shrub land were the main species, which mainly distributed in two mountain areas (Fangdou Mountain, Qiyao Mountain) affected by topographic restriction and woodland natural basis. The woodland terrain niche distribution index showed an overall upward trend with increasing terrain niche gradient, especially for the arbor woodland and shrub land, while the other woodland types presented an opposite trend. The percentage of woodland area occupying the corresponding buffer radius around the roads, waterbodies and settlements had a strong similarity with the woodland terrain niche distribution index. Only around the settlements, bamboo forest, sparse woodland and immature woodland occupied higher percentages of the woodland area of the corresponding buffer radius than that of arbor woodland and shrub land. Woodland distribution was mainly controlled by large landform patterns of mountain features, while the distribution of woodlands in the different terrain niches and the different buffer radii of roads, waterbodies and settlements were driven mainly by duress of human activities under the auspices of the large landform patterns.

[Predictive value of single and combined indexes in the diagnosis of sarcoidosis].

OBJECTIVE: To evaluate the predictive value of single and combined indexes in the diagnosis of sarcoidosis. METHODS: Eighty-eight patients suspected of having sarcoidosis were retrospectively investigated. The diagnostic predictive value of lymphocyte percentage and CD4/CD8 ratio in bronchoalveolar lavage (BAL), angiotensin converting enzyme (ACE) and their combination was evaluated by ROC curve method and Bayes' rule. RESULTS: Final diagnosis of sarcoidosis by biopsy was available in 59 patients (67%), 16 were males (27%), 43 were females (73%), with a mean age of (48 +/- 10) years. The diagnosis of non-sarcoidosis was available in 29 patients (33%), 12 were males (41%), 17 were females (59%), with a mean age of (49 +/- 13) years. The ROC curve area of lymphocyte percentage, CD4/CD8 ratio and ACE were 0.64, 0.74 and 0.69 respectively; CD4/CD8 ratio had the best diagnostic efficiency. The optimized cut-offs of the three single indexes, lymphocyte percentage > or = 30%, CD4/CD8 ratio > or = 4.0 and ACE > or = 40 U/L, were determined by the tangential points and coordinates of the ROC curve. The positive predictive values were 76.7%, 80.4% and 76.8% respectively; CD4/CD8 ratio had the best predictive value. The ROC curve area of the combined CD4/CD8 ratio and ACE was 0.81, and the combination of lymphocyte percentage, CD4/CD8 ratio and ACE was 0.78. They were both higher than those of the respective single indexes. The combination of CD4/CD8 ratio and ACE had the best diagnostic efficiency. On the other hand, it had the best positive predictive value of 90.5% in all the diagnostic indexes. CONCLUSION: CD4/CD8 ratio in BAL is still a useful index in the auxiliary diagnosis of sarcoidosis. The combination of CD4/CD8 ratio and ACE can improve the diagnostic efficiency and predictive value of sarcoidosis.

Invasiveness and intracellular growth of multidrug-resistant salmonella and other pathogens in Caco-2 cells.

The increase of multidrug-resistant pathogens of human and animal origins is a major public health concern. For a better understanding of the health consequences of multidrug-resistant bacteria transmitted from animal products to humans, the host interaction of zoonotic Salmonella isolates along with other pathogenic and commensal bacteria was evaluated using a human intestinal Caco-2 cell system. Multidrug-resistant S. Agona, S. Heidelberg, and S. Typhimurium possessed plasmid-mediated class 1 integrons. The S. Typhimurium DT104 isolate from ground beef showed the well-known genotypic and phenotypic resistance characteristics of the species, and contained the chromosomally located class 1 integron. Among the multidrug-resistant Salmonella isolates, the S. Heidelberg 219 had the highest invasion number at 1.0 x 10(4) CFU/mL, followed by the S. Typhimurium DT104 isolate at 7.7 x 10(3) CFU/mL. Listeria monocytogenes was the best performer among the tested species in invading the Caco-2 cell. Multidrug-resistant opportunistic pathogens Klebsiella pneumoniae and Pseudomonas aeruginosa were also able to invade the cells. The invasion of S. Heidelberg 219, S. Typhimurium DT104, L. monocytogenes, K. pneumoniae, and P. aeruginosa into the Caco-2 cells was not affected even in the presence of commensal E. coli. During the intracellular growth of S. Heidelberg 219, S. Typhimurium DT104, and L. monocytogenes, the bacterial counts increased 2 log cycles in 9 h in the Caco-2 cells. Therefore, these strains could rapidly proliferate after their invasion into the cells.

Expression of AmpC beta-lactamase in Enterobacter cloacae isolated from retail ground beef, cattle farm and processing facilities.

AIMS: To better understand antibiotic resistance of Enterobacter cloacae isolates originated from food animals, the phenotypic and genotypic resistance of Ent. cloacae isolates from retail ground beef, cattle farm, processing facilities and clinical settings were investigated. METHODS AND RESULTS: The ampC, ampD and ampR genes in the isolates were sequenced and analysed. beta-Lactamase activities and beta-lactamase profiles of the isolates were analysed by the enzymatic hydrolysis of nitrocefin and isoelectric focussing, respectively. The ampC gene of the Ent. cloacae isolate was cloned and transformed into Escherichia coli strains. The genomic DNA profiles of Ent. cloacae isolates were analysed by using pulse field gel electrophoresis (PFGE). Mutation at one residue (Val-54-->Ile) in the AmpR amino acid sequence was consistently found in Ent. cloacae isolates that were resistant to a broadspectrum of beta-lactam agents. The enzyme activity in the isolates was induced by cefoxitin. The pI (isoelectric point) of the enzymes produced by the test strains ranged from 8.4 to 8.9. Cloning of ampC gene of the Ent. cloacae isolate conferred the resistance to ampicillin, cephalothin and amoxicillin in recipient E. coli strains. One recipient of E. coli O157:H7 strain additionally acquired resistance to ceftiofur. The genomic analysis of Ent. cloacae isolates by PFGE showed that the isolates from various sources were genetically unrelated. CONCLUSIONS: The spread of diverse clones of AmpC-producing Ent. cloacae occurred in the ecosystem and retail products. SIGNIFICANCE AND IMPACT OF THE STUDY: Our findings suggested that AmpC-producing Ent. cloacae could be a contributor in spreading beta-lactamase genes in farm environments and food processing environments.

[Analysis of the treatment and prognosis of 59 patients with sarcoidosis].

OBJECTIVE: To investigate the relationship between clinical characteristics, treatment and prognosis of sarcoidosis, and to analyze the factors for disease severity and prognosis. METHOD: Retrospective analysis of 59 patients with sarcoidosis admitted to Peking Union Medical College Hospital from January 1984 to January 2003, with a follow-up for at least 24 months. RESULTS: The mean follow-up period was (61.9 +/- 49.4) months. All of the 7 patients with stage 0 accepted glucocorticoid therapy and responded well, and 4 of them completely remitted. Among 22 cases with stage I disease, 4 underwent spontaneous remission while another one who had extra-pulmonary sarcoidosis at the onset progressed after follow-up for 6 months. Seventeen cases were treated with glucocorticoids after the diagnosis was confirmed. Before this study, 9 patients had experienced complete remission, and 7 had been stable disease respectively, while one had progressed to stage II. Twenty-two of the 23 stage II patients received glucocorticoids initially and one was treated after an observation period of 3 months before deterioration. Thirteen patients of stage II were completely cured, and 2 were still progressing under the drug therapy. All of the 5 patients in stage III accepted glucocorticoids after diagnosis. One of the 5 patients has resolved completely and 2 had progressed into stage IV. The interstitial changes of the lung in 2 stage IV patients had been progressing even though the treatment lasted for years. There were no significant differences in the serum angiotensin converting enzyme (ACE) level, total broncho-alveolar fluid (BALF) cell count, percentage of alveolar lymphocytes, neutrophils and lymphocyte CD(4)/CD(8) ratio at the onset between stage I and stage II diseases. Age, gender, presence of respiratory symptom, serum ACE level, total BALF cell count, percentage of alveolar lymphocytes, neutrophils or lymphocyte CD(4)/CD(8) ratio at the onset were not associated with relapse. 14 cases relapsing pulmonary sarcoidosis had extra-pulmonary lesions, and there was a close correlation between the relapse and extra-pulmonary lesions (P = 0.006). A significant increase in the percentage of BALF neutrophils (8.37 +/- 3.55)% at the time of diagnosis was observed in patients who had not completely resolved as compare with the patients who had recovered [(3.52 +/- 2.37)%, P < 0.001]. CONCLUSION: The outcome of sarcoidosis are generally good. Patients with extra-pulmonary sarcoidosis are more likely to experience relapse. Stage I disease should receive observation before glucocorticoid therapy. Increased percentage of neutrophils in BALF at the onset is possibly associated with a progressive or persistent disease.

Recombinant human prolactin protects against irradiation-induced myelosuppression.

Prolactin is a multifunctional hormone that exerts many separate functions and acts as an important connection between the endocrine and immune systems. There are increasing researches implicating the role of prolactin in hematopoiesis. Enhanced erythropoiesis in pregnant women and direct erythropoietic effects in vitro of plasma either from pregnant or lactating mice have been reported. Furthermore, regression of erythroblastic leukemia has been observed in a significant number of rats after hypophysectomy. In this study, the effects of recombinant human prolactin (rhPRL) on hematopoiesis were assessed in irradiated mice. Mice were treated with rhPRL for five consecutive days after exposure to a lethal dose or a sub-dose irradiation. Prolonged survival rate and increased erythropoiesis were observed in the irradiation-induced myelosuppressive mice. It was concluded that rhPRL might act on erythropoiesis and could be a potential candidate for the treatment of irradiation-induced myelosuppresion in clinic.

[Study of the gene deletions and the immunofluorescence of muscle in patients with DMD/BMD].

AIM: To detect the deletion distribution of dystrophin gene and dystrophin changes in muscle cells of the patients with Duchenne/Becker muscular dystrophy (DMD/BMD), furthermore to investigate the relationship between them and clinical symptoms. METHODS: 42 patients with DMD/BMD were screened by 9 primers multiplex PCR. The patients from 5 DMD and 2 BMD were detected by immunofluorescence technique for analyzing dystrophin located in muscle cell membrane, compared with 2 normal males. RESULTS: The deletion of one or more exons was found in 21 patients. 16 cases (76.2%) were detected in the central region and 5 patients (23.8%) in the 5' extreme region, especially in exon 48 (6 patients). Negative result of staining was seen in 5 DMD patients. Of these, one case of DMD had no detectable levels of dystrophin, but no deletion of DMD gene. Dystrophin immunostaining from two BMD patients consisted of a discontinuous staining pattern around most fibers. CONCLUSION: It might be possible that some correlation existed between the type of gene deletion and the degree of severity of the disease. The amount and size of exon deletion may not affect the symptoms. DMD/BMD are highly heterogeneous in clinical manifestation and in inheritance pattern. The pathologic foundation of DMD and BMD is the absence or abnormal expression of dystrophin. The consequence of that depends not only on the degree, but also on the function.