Biofeedback therapy combined with Baduanjin on quality of life and gastrointestinal hormone level in patients with colorectal cancer.

BACKGROUND: With the change in people's lifestyles, the incidence of colorectal cancer (CRC) is increasing. It is essential to study the efficacy of various treatment methods for CRC patients to prevent and treat CRC. AIM: To investigate the efficacy of biofeedback therapy combined with Baduanjin in improving the quality of life and gastrointestinal hormone levels of patients with CRC. METHODS: A total of 120 patients with CRC who were admitted to our hospital from June 2020 to June 2021 were included in the study. They were randomly divided into four groups (n = 30): the control group (group A), the biofeedback therapy intervention group (group B), the Baduanjin exercise intervention group (group C), and the combination group (group D). Patients in group A adopted the standard nursing mode and necessary health education. Patients in group B were treated with biofeedback therapy based on routine nursing care. Patients in group C were given Baduanjin intervention for 12 wk based on conventional drug treatment and care. Patients in group D were treated with biofeedback therapy and Baduanjin exercise. In this study, patients' quality of life, gastrointestinal hormone levels, and clinical efficacy in the four groups were observed at baseline and 12 wk after intervention. Meanwhile, the correlation between gastrointestinal hormone levels and various functional areas of quality of life was analyzed. By comparing the observed indicators of patients in the four groups, the efficacy of biofeedback therapy combined with Baduanjin in improving the quality of life and gastrointestinal hormone levels of patients with CRC was explored. RESULTS: At baseline, there were no significant differences in quality of life, gastrointestinal hormone levels, or clinical efficacy among the four groups (P > 0.05). Twelve weeks after the intervention, the combination group's quality of life, gastrointestinal hormone levels, and clinical effectiveness were better than those of the three other groups. CONCLUSION: On the basis of routine nursing care, patients with CRC combined with biofeedback therapy and Baduanjin exercise can improve the quality of life of patients with CRC and the efficacy of gastrointestinal hormone levels.

[Regulatory effect of FK506 on the expression of CD152 and PD-1 in the liver allo-recipients].

AIM: To dynamically observe the expression of CTLA-4/CD152 and PD-1 on T cell surface in the peripheral blood of liver allo-recipients, and to explore the regulatory effect of FK506 on negative costimulatory molecules. METHODS: The blood concentration of FK506 was measured by enzyme-multiplied immunoassay technique. Flow cytometry (FCM) was used to determine the expression of T cell subsets and CD152, PD-1 on T cell surface in the peripheral blood. RESULTS: There was no significant difference in blood concentration of FK506 between each group (P > 0.05). With the progression of treatment, the frequency of CD4(+) T cells gradually decreased and was obviously lower than that in health control until the third month (P < 005). The expression of CD4(+) T cells in each treatment group was significantly lower than that in disease control group (P < 0.05). And the frequency of CD8(+) T cells in each treatment group was obviously higher than that in disease control group (P < 0.05). After liver transplantation, the expression of CD152 on CD4(+) and CD8(+) T cells was higher than that in health control group (P < 0.05), and the expression in the second week and first month was higher than that in disease control group (P < 0.05). The frequencies of CD4(+) CD152(+) T cells in the second and third month were lower than that in the second week and first month (P < 0.05). The frequency of CD8(+) CD152(+) T cells in the third month was lower than that in the second week and first month (P < 0.05). After liver transplantation, the expression of PD-1 on T cell subsets had the tendency of advancing. Its expression on CD4(+) T cells was significantly higher than that in health control group from the second week (P < 0.05), and the expression on CD8(+) T cells increased obviously from the first month compared with that in disease control group (P < 0.05). CONCLUSION: FK506 could up-regulate the expression of negative costimulatory molecules CD152 and PD-1 on T cell surface and inhibit the proliferation and activation of effector T cells, which may contribute to maintain the survival and homeostasis of allo-recipients.

[Analysis of Foxp3+ CD4+ CD25+ regulatory cells in peripheral blood of patients with SLE].

AIM: To investigate the expression of GITR on T cells and the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood of patients with SLE. METHODS: The expression of GITR and Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood was determined by flow cytometry(FCM). RESULTS: Compared with health control, the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in peripheral blood from SLE patients was lower (P<0.05), but there is no difference in the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells between active and inactive SLE patients (P>0.05). GITR expression on both CD3(+) CD4(+) T cells and CD3(+) CD8(+) T cells of SLE patients increased significantly (P>0.05). With the development of SLE activity, GITR expression didn't change significantly on both CD3(+) CD4(+) T cells (P>0.05) and CD3(+) CD8(+) T cells (P>0.05). CONCLUSION: The expression abnormality of both Foxp3(+) CD4(+) CD25(+) regulatory cells and GITR may play important role in the imbalance of immune homeostasis in SLE.