The OsWRKY72-OsAAT30/OsGSTU26 module mediates reactive oxygen species scavenging to drive heterosis for salt tolerance in hybrid rice.

Hybrid rice (Oryza sativa) generally outperforms its inbred parents in yield and stress tolerance, a phenomenon termed heterosis, but the underlying mechanism is not completely understood. Here, we combined transcriptome, proteome, physiological, and heterosis analyses to examine the salt response of super hybrid rice Chaoyou1000 (CY1000). In addition to surpassing the mean values for its two parents (mid-parent heterosis), CY1000 exhibited a higher reactive oxygen species scavenging ability than both its parents (over-parent heterosis or heterobeltiosis). Nonadditive expression and allele-specific gene expression assays showed that the glutathione S-transferase gene OsGSTU26 and the amino acid transporter gene OsAAT30 may have major roles in heterosis for salt tolerance, acting in an overdominant fashion in CY1000. Furthermore, we identified OsWRKY72 as a common transcription factor that binds and regulates OsGSTU26 and OsAAT30. The salt-sensitive phenotypes were associated with the OsWRKY72paternal genotype or the OsAAT30maternal genotype in core rice germplasm varieties. OsWRKY72paternal specifically repressed the expression of OsGSTU26 under salt stress, leading to salinity sensitivity, while OsWRKY72maternal specifically repressed OsAAT30, resulting in salinity tolerance. These results suggest that the OsWRKY72-OsAAT30/OsGSTU26 module may play an important role in heterosis for salt tolerance in an overdominant fashion in CY1000 hybrid rice, providing valuable clues to elucidate the mechanism of heterosis for salinity tolerance in hybrid rice.

Robust Point Cloud Registration Network for Complex Conditions.

Point cloud registration is widely used in autonomous driving, SLAM, and 3D reconstruction, and it aims to align point clouds from different viewpoints or poses under the same coordinate system. However, point cloud registration is challenging in complex situations, such as a large initial pose difference, high noise, or incomplete overlap, which will cause point cloud registration failure or mismatching. To address the shortcomings of the existing registration algorithms, this paper designed a new coarse-to-fine registration two-stage point cloud registration network, CCRNet, which utilizes an end-to-end form to perform the registration task for point clouds. The multi-scale feature extraction module, coarse registration prediction module, and fine registration prediction module designed in this paper can robustly and accurately register two point clouds without iterations. CCRNet can link the feature information between two point clouds and solve the problems of high noise and incomplete overlap by using a soft correspondence matrix. In the standard dataset ModelNet40, in cases of large initial pose difference, high noise, and incomplete overlap, the accuracy of our method, compared with the second-best popular registration algorithm, was improved by 7.0%, 7.8%, and 22.7% on the MAE, respectively. Experiments showed that our CCRNet method has advantages in registration results in a variety of complex conditions.

Biofertilizer based on halotolerant microorganisms promotes the growth of rice plants and alleviates the effects of saline stress.

Long-term soil salinization easily contributes to soil hardness, soil nutrient imbalance, and soil microbial diversity reduction, resulting in low rice yields in the salinized fields, and microbial remediation is one of the important measures to improve salinized soil. To verify the effect of biofertilizer based on halotolerant microorganisms on promoting rice growth and alleviating saline stress, this study discussed the effects of biofertilizer on soil microbial diversity and community structure and analyzed the correlation between the formation of microbial community structure and soil nutrient factors in the salinized field. The result, in comparison with applying inorganic fertilizer (referred to as CK), showed that notably increased soil available nitrogen, available phosphorus, available potassium, and rice paddy yield (p < 0.05) and significantly decreased soil electrical conductivity (p < 0.05) were achieved via biofertilizer (referred to as G2). Additionally, the application of biofertilizer contributes to the increase in soil microbial diversity and reorganization of microbial community structure, and through the analysis of linear discriminant analysis effect size, a notable difference in relative abundance was found in 13 genera, 6 families, and 3 orders between the control group and experimental groups (p < 0.05), and by linear discriminant analysis, Desulfomonas was further identified as the differentiated indicator. The redundancy analysis showed that available phosphorus and cation exchange capacity were the key environmental factors that affected microbial community structure and composition. Through bacterial functional prediction, increased rhizosphere soil bacterial metabolism, enzyme activity, membrane transport, and other potential functions were achieved by applying biofertilizer. Therefore, the application of biofertilizer could significantly alleviate rice growth stress and increase nutrient supply capacity in saline soil. These findings provide theoretical support for soil microbial improvement technology in the salinized field.

Superwetting membrane-based strategy for high-flux enrichment of ethanol from ethanol/water mixture.

Ethanol, which can be scalable produced from fermented plant materials, is a promising candidate to gasoline as the next-generation liquid fuel. As an energy-efficient alternative to distillation, membrane-based strategies including pervaporation and reverse osmosis have been developed to recover ethanol from fermentation broths. However, these approaches suffer the drawback of low separation flux. Herein, we report a superwetting membrane system to enrich ethanol from water in a high-flux manner. By synergistically regulating surface energy of the solid porous membrane and hydration between an additive inorganic potassium salt and water, concentrated ethanol can rapidly wetting and permeate the porous membrane, with the salt solution being blocked. Using this newly developed superwetting membrane system, we can achieve fast enrichment of ethanol from water, with flux of two orders magnitude higher than that of pervaporation and reverse osmosis membranes.

Plant-atmosphere and soil-atmosphere temperature differences and their impact on grain yield of super hybrid rice under different irrigation conditions.

Continued drought during the late growth stage of super hybrid rice (SHR) markedly reduces yield, and management practices that use water more efficiently can contribute greatly to high and stable yields from SHR. The absolute temperature differences (ATDs) between the rice plant and the atmosphere and between the soil and the atmosphere are believed to be important determinants of grain yield. However, it has not previously been determined whether these ATDs have any effect on SHR yields under water-saving cultivation. A two-year field experiment involving two SHR varieties, Liangyoupeijiu (LYPJ) and Y-Liangyou 9000 (YLY900), evaluated the effects of reducing water supply from mid-booting to maturity on grain yield, canopy relative humidity (CRH), leaf area index (LAI), and ATDs between the ambient temperature and the leaf surface, panicles, canopy, and soil. Grain yield increased significantly under shallow water irrigation (SW), by 8.84% (YLY900) and 12.26% (LYPJ), but decreased significantly under mild water stress (MS, -20 to -30 kPa), by 14.36% (YLY900) and 9.47% (LYPJ), as well as severe water stress (SS, -40 to -50 kPa), by 35.06% (YLY900) and 28.74% (LYPJ). As water supply decreased, so did the CRH and the ATDs, with significant decreases under MS and SS. The temperature differences were significantly and positively correlated with grain yield (P < 0.01) in both cultivars. LAI was increased under SW conditions, but was significantly decreased under MS and SS. Our study suggests that the dual goal of saving water while maintaining high yield can be achieved by applying SW irrigation from mid-booting to maturity and by adopting cultivation measures that maintain high CRH and high plant-atmosphere and soil-atmosphere ATDs in order to alleviate water stress. YLY900 has a higher yield potential than LYPJ under SW conditions, suggesting that its wide cultivation may help achieve this dual goal.

Antigen-oriented T cell migration contributes to myelin peptide induced-EAE and immune tolerance.

Treatment with soluble myelin peptide can efficiently and specifically induce tolerance to demyelination autoimmune diseases including multiple sclerosis, however the mechanism underlying this therapeutic effect remains to be elucidated. In actively induced mouse model of experimental autoimmune encephalomyelitis (EAE) we analyzed T cell and innate immune cell responses in the central nervous system (CNS) and spleen after intraperitoneal (i.p.) infusion of myelin oligodendrocyte glycoprotein (MOG). We found that i.p. MOG infusion blocked effector T cell recruitment to the CNS and protected mice from EAE and lymphoid organ atrophy. Innate immune CD11b(+) cells preferentially recruited MOG-specific effector T cells, particularly when activated to become competent antigen presenting cells (APCs). During EAE development, mature APCs were enriched in the CNS rather than in the spleen, attracting effector T cells to the CNS. Increased myelin antigen exposure induced CNS-APC maturation, recruiting additional effector T cells to the CNS, causing symptoms of disease. MOG triggered functional maturation of splenic APCs. MOG presenting APCs interacted with MOG-specific T cells in the spleen, aggregating to cluster around CD11b(+) cells, and were trapped in the periphery. This process was MHC II dependent as an MHC II directed antibody blocked CD4(+) T cell cluster formation. These findings highlight the role of myelin peptide-loaded APCs in myelin peptide-induced EAE and immune tolerance.