RESUMO
We conducted a case-control study in a Chinese population, and assessed whether the VEGF -634G/C, +936C/T, and +1612G/A polymorphisms could affect the risk of osteosarcoma and its association with environmental factors. A total of 180 consecutive osteosarcoma patients and 360 controls were included in our study. The genotypes of VEGF -634G/C, +936C/T, and +1612G/A were determined by the polymerase chain reaction-restriction fragment length polymorphism assay. Conditional logistic regression analyses showed that subjects carrying the GG genotype and the G allele of VEGF -634G/C were significantly associated with increased risk of osteosarcoma compared to those with the CC genotype; the ORs (95%CIs) were 2.28 (1.31-3.96) and 1.51 (1.16-1.97) for the GG genotype and G allele, respectively. We found that the GG genotype of VEGF -634G/C was associated with a significantly increased risk of osteosarcoma in patients of either gender with younger age and a family history of cancer. In summary, this study found that the VEGF -634G/C gene polymorphism was associated with an increased risk of osteosarcoma.
Assuntos
Estudos de Associação Genética , Predisposição Genética para Doença , Osteossarcoma/genética , Fator A de Crescimento do Endotélio Vascular/genética , Adulto , Idoso , Alelos , Povo Asiático , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Osteossarcoma/patologia , Polimorfismo de Nucleotídeo ÚnicoRESUMO
The effects of foliar spraying with spermidine (Spd) on the subcellular distribution and chemical speciation of Cd in Typha latifolia L. in hydroponic system were investigated. Increased Cd concentration in the medium caused an increase of Cd concentration in all tissues and all subcellular fractions, with the highest Cd level occurring in roots, followed by rhizomes and leaves and the greatest accumulation was in cell walls, soluble fraction and organelle containing fractions ranked next, respectively. The greatest amount of Cd was found in the extraction of 1 mol.L(-1) NaCl, followed by 2% HAC, deionized water, 80% ethanol, residues and the least in 0.6 mol.L(-1) HCl. The subcellular distribution and different chemical forms of Cd both decreased after Spd application and were more inhibited by 0.25 than 0.50 mmol.L(-1) Spd, especially in roots at 0.10 mmol.L(-1) Cd treatment. It could be suggested that the retention of Cd in roots, cell walls binding with metal, metal ion compartmentation in vacuoles and Cd forms existing in undissolved and integrated with pectates and protein play an important role in Cd detoxification and Spd could enhance the tolerance of cattail to Cd stress.
Assuntos
Biodegradação Ambiental , Cádmio/farmacocinética , Poluentes Ambientais/farmacocinética , Espermidina/farmacologia , Typhaceae/química , Cádmio/metabolismo , Cádmio/toxicidade , China , Poluentes Ambientais/metabolismo , Poluentes Ambientais/toxicidade , Hidroponia , Espermidina/metabolismoRESUMO
OBJECTIVE: The optimal biosynthesis of L-PAC by immobilized Sc-5 yeast cell was systematically studied in this paper. METHOD: Biosynthesis. RESULT AND CONCLUSION: The average of L-PAC yield produced by the immobilized Sc-5 yeast cell is > or = 2 g.L-1 and half life > or = 240 h.
