Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water.

Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-shaped probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli. In addition, another methos of detection was tested where the sensors were calibrated in the air, before being immersed in a bacterial suspension and then read in the air. This modification improved sensor sensitivity and resulted in a faster detection time. After the immunocapture, the sensors were DAPI-stained and submitted to confocal microscopy. The histograms obtained confirmed that the responses of the immunosensors were due to the bacteria. This new sensor detected the presence of E. coli at 104 CFU/mL in less than 20 min. Currently, sub-20 min is faster than previous studies using fiber-optic based biosensors. We report on an inexpensive and faster detection technology when compared with conventional methods.

Optical Sensor for Monitoring Leakage Current and Weather Conditions in a 500-kV Transmission Line.

The leakage current (LC) caused by the surface contamination of insulators, together with environmental variables, is one of the most basic online monitoring parameters for insulator status. However, the impact of weather conditions such as temperature, air humidity, and dew point on the LC has not been deeply studied until now. In this paper, based on meteorological data obtained online and LC obtained with an optical fiber sensor, installed in 500-kV insulator strings of a transmission line, the impact of weather conditions was studied. Results indicate that the LCs follow a specific pattern, according to weather conditions. The system has been continuously monitoring LC, humidity, temperature, and dew point uninterrupted for three years, sending the acquired data to a web page; therefore, it has been demonstrated to be robust, reliable, and repetitive. The sensor features the broadband response and acquisition capabilities of partial discharge pulses in high-voltage insulators, allowing the detection of high-frequency pulses. When comparing the LC measured in this work with those from other works, our measurements are substantially higher; this is due to the type of pollution found in this specific situation, which includes iron oxide powder, producing a conductive layer over the insulator surface that, unlike sea salt, does not depend on humidity to conduct an LC. One of the conclusions reached in this work is that partial discharge surges are caused when the local temperature reaches the dew point and not simply from the presence of high humidity, as stated in many works dealing with LCs. The monitored LC can be used as an indicative parameter of a possible flashover, enabling the proper planning of insulator predictive maintenance, either by jet-washing the surface or even changing the insulators when they are damaged.

Delayed left main coronary obstruction following transfemoral inovare transcatheter aortic valve replacement: A challenging case.

Coronary obstruction is an uncommon and severe complication after a transcatheter aortic valve replacement (TAVR), that occurs during the procedure in the vast majority of patients. In the present case even in the absence of classic risk factors, an acute coronary syndrome occurred one day after TAVR. Selective angiography revealed a severe left main ostium obstruction by the bulky native leaflet calcification. This is the first case of delayed presentation of coronary obstruction with a transfemoral balloon-expandable valve using the Inovare bioprosthesis (Braile Biomedica, Brazil). In addition, after drug-eluting stent placement in the left main coronary, intravascular ultrasound revealed severe stent underexpansion, so that a second layer of a bare-metal stent and high-pressure balloon post-dilatation was necessary to improve the final result. The patient was discharged after 7 days, and at the 6-month follow-up remained asymptomatic. .

Localized surface plasmon resonance-based biosensor on gold nanoparticles for Taenia solium detection.

This paper describes, for the first time to our knowledge, a fast-response and specific biosensor for detection of Taenia solium, a parasite responsible for neurocysticercosis disease that affects the central nervous system. The biosensor is based on the localized surface plasmon resonance (LSPR) technique on gold nanoparticles (AuNPs) in colloidal suspension that were functionalized and activated with antibodies to perform an immuno-capture effect. The AuNPs were synthetized by Turkevich and seed-mediated growth methods. A variety of concentrations of T. solium antigen were added to test the detection and the dose-response profile. Small antigen concentrations were detected indicating that the limit of detection is lower than 0.1 µg/mL of antigen. The results demonstrate the potential of the AuNPs LSPR biosensor as a clinical tool for neurocysticercosis diagnostic.

Optical Voltage Transformer Based on FBG-PZT for Power Quality Measurement.

Optical Current Transformers (OCTs) and Optical Voltage Transformers (OVTs) are an alternative to the conventional transformers for protection and metering purposes with a much smaller footprint and weight. Their advantages were widely discussed in scientific and technical literature and commercial applications based on the well-known Faraday and Pockels effect. However, the literature is still scarce in studies evaluating the use of optical transformers for power quality purposes, an important issue of power system designed to analyze the various phenomena that cause power quality disturbances. In this paper, we constructed a temperature-independent prototype of an optical voltage transformer based on fiber Bragg grating (FBG) and piezoelectric ceramics (PZT), adequate to be used in field surveys at 13.8 kV distribution lines. The OVT was tested under several disturbances defined in IEEE standards that can occur in the electrical power system, especially short-duration voltage variations such as SAG, SWELL, and INTERRUPTION. The results demonstrated that the proposed OVT presents a dynamic response capable of satisfactorily measuring such disturbances and that it can be used as a power quality monitor for a 13.8 kV distribution system. Test on the proposed system concluded that it was capable to reproduce up to the 41st harmonic without significative distortion and impulsive surges up to 2.5 kHz. As an advantage, when compared with conventional systems to monitor power quality, the prototype can be remote-monitored, and therefore, be installed at strategic locations on distribution lines to be monitored kilometers away, without the need to be electrically powered.

Detection of Bacillus anthracis and Bacillus anthracis-like spores in soil from state of Rio de Janeiro, Brazil.

BACKGROUND: Bacillus anthracis is the aetiologic agent of anthrax, a re-emerging, septicaemic, haemorrhagic and lethal disease that affects humans, domestic ruminants and wildlife. Plasmids pXO1 and pXO2 are attributes that confer pathogenicity to B. anthracis strains. This bacterium was used as biological weapon in the World Wars and in the biological attack in the United States of America at 2001. B. anthracis is classified as a Tier 1 bioterrorism agent by the Centers for Diseases Control and Prevention. Anthrax is recognised as a re-emerging disease. Several studies concerning the dynamics of B. anthracis cycle in soil revealed that nonpathogenic B. anthracis strains due to lack of pXO2 plasmid are commonly found in some types of soil. OBJECTIVES: This study aimed isolation and identification of B. anthracis spores in soil samples of the state of Rio de Janeiro, Brazil. METHODS: Phenotypic and genotypic approaches were used to identify isolates including MALDI-TOF/MS, motility test, susceptibility to gamma phage and penicillin, survey for pag and cap genes as surrogates of pXO1 and pXO2 plasmids, respectively, and sequencing of 16SrRNA-encoding gene. Physicochemical analysis of the soil samples were carried out to describe soil characteristics. FINDINGS: We observed the presence of one B. anthracis pXO1+ and pXO2- isolated from clay loam soil; one B. anthracis-like strain pXO1+ and pXO2-isolated from loamy sand; and 10 Bacillus spp. strains sensitive to phage-gamma that need better characterisation to define which their species were recovered from loamy sand. MAIN CONCLUSIONS: This work showed promising results and it was the first study to report results from an active surveillance for B. anthracis in Brazil.

Optical high-voltage sensor based on fiber Bragg gratings and stacked piezoelectric actuators for a.c. measurements.

In this paper, we present the design and fabrication of a compact, modular optical high-voltage sensor (OHVS) based on fiber Bragg gratings (FBG) for a.c. distribution and transmission lines. The proposed OHVS is composed by a stack of piezoelectric transducers that transfer mechanical strain to a sensing FBG. A prototype was tested in the laboratory and showed a maximum linearity error of less than 3% of full-scale range (FSR) for input voltages up to ${14\,\,{\rm kV}_{\rm rms}}$14kVrms with a signal-to-noise ratio of 55 dB, allowing measurements with a resolution of less than 0.2% of FSR. Transient response of the developed OHVS was preliminarily investigated, and a response time of less than 1 ms was observed. The results obtained allow us to conclude that the developed OHVS may also be used for the detection of transient events.

Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors.

Escherichia coli (E. coli) is a large and diverse bacteria group that inhabits the intestinal tract of many mammals. Most E. coli strains are harmless, however some of them are pathogenic, meaning they can make one sick if ingested. By being in the feces of animals and humans, its presence in water and food is used as indicator of fecal contamination. The main method for this microorganism detection is the bacterial culture medium that is time-consuming and requires a laboratory with specialized personnel. Other sophisticated methods are still not fast enough because they require sending samples to a laboratory and with a high cost of analysis. In this paper, a gold-coated U-shaped plastic optical fiber (POF) biosensor for E. coli bacteria detection is presented. The biosensor works by intensity modulation principle excited by monochromatic light where the power absorption is imposed by predominant effect of either bending loss or surface plasmon resonance (SPR), depending on the gold thickness. Bacterial selectivity is obtained by antibody immobilization on the fiber surface. The biosensor showed a detection limit of 1.5 × 10³ colony-forming units (CFU)/mL, demonstrating that the technology can be a portable, fast response and low-cost alternative to conventional methodologies for quality analysis of water and food.

Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results.

Conventional pathogen detection methods require trained personnel, specialized laboratories and can take days to provide a result. Thus, portable biosensors with rapid detection response are vital for the current needs for in-loco quality assays. In this work the authors analyze the characteristics of an immunosensor based on the evanescent field in plastic optical fibers with macro curvature by comparing experimental with simulated results. The work studies different shapes of evanescent-wave based fiber optic sensors, adopting a computational modeling to evaluate the probes with the best sensitivity. The simulation showed that for a U-Shaped sensor, the best results can be achieved with a sensor of 980 µm diameter by 5.0 mm in curvature for refractive index sensing, whereas the meander-shaped sensor with 250 µm in diameter with radius of curvature of 1.5 mm, showed better sensitivity for either bacteria and refractive index (RI) sensing. Then, an immunosensor was developed, firstly to measure refractive index and after that, functionalized to detect Escherichia coli. Based on the results with the simulation, we conducted studies with a real sensor for RI measurements and for Escherichia coli detection aiming to establish the best diameter and curvature radius in order to obtain an optimized sensor. On comparing the experimental results with predictions made from the modelling, good agreements were obtained. The simulations performed allowed the evaluation of new geometric configurations of biosensors that can be easily constructed and that promise improved sensitivity.

Odontological light-emitting diode light-curing unit beam quality.

The distribution of light intensity of three light-curing units (LCUs) to cure the resin-based composite for dental fillings was analyzed, and a homogeneity index [flat-top factor (FTF)] was calculated. The index is based on the M2 index, which is used for laser beams. An optical spectrum analyzer was used with an optical fiber to produce an x-y power profile of each LCU light guide. The FTF-calculated values were 0.51 for LCU1 and 0.55 for LCU2, which was the best FTF, although it still differed greatly from the perfect FTF = 1, and 0.27 for LCU3, which was the poorest value and even lower than the Gaussian FTF = 0.5. All LCUs presented notably heterogeneous light distribution, which can lead professionals and researchers to produce samples with irregular polymerization and poor mechanical properties.

Plastic optical fiber-based biosensor platform for rapid cell detection.

This work presents a novel, fast response time, plastic optic fiber (POF) biosensor to detect Escherichia coli. It discloses the technique for the development, calibration and measurement of this robust and simple-to-construct POF biosensor. The probes in U-shaped format were manufactured with a specially developed device. The calibration process led to the evaluation of the sensitivity, accuracy and repeatability by using solutions of sucrose for obtaining refractive indices (RI) in the range 1.33-1.39 IR equivalent of water and bacteria, respectively. The POF probes were functionalized with antibody anti-E. coli serotype O55 and tested firstly with saline and then with bacterial concentrations of 10(4), 10(6), and 10(8) colony forming units/ml (CFU/ml). The optoelectronic setup consists of an 880 nm LED connected to the U-shaped probe driven by a sine waveform generated by the Simulink (from Matlab(®)). On the other side of the probe a photodetector generates a photocurrent which is amplified by a transconductance amplifier. The output voltage signal is read by the analog-to-digital (A/D) input of the microcontroller. In all tested concentrations, the results presented a tendency of a decrease in the output signal with time, due to the attachment of the bacteria to the POF probe and consequent increase in the RI close to the sensitive area of the fiber surface. It has been shown that the system is capable of providing positive response to the bacterial concentration in less than 10 min, demonstrating good possibilities to be commercially developed as a portable field sensor.

Tapered plastic optical fiber-based biosensor--tests and application.

Cells detection is crucial in microbiological analysis of clinical, food, water or environmental samples. However, currently employed methods are time consuming. Plastic optical fiber (POF) biosensors consist in a viable alternative for rapid and inexpensive scheme for detection. In order to study the sensitivity of tapers for microbiological detection, geometric parameters are studied, such as the taper waist diameter since the formation of taper regions are the key sensing element in this particular type of sensors. In this study, a series of POF taper sensors were prepared using a specially developed tapering machine, and the dispersion of geometric dimensions is evaluated, aiming to achieve the best tapering characteristics which will provide a better sensitivity on the sensor response. The fiber tapers that presented the finest results were those constructed in U-shaped (bended) configurations, with taper waist diameters ranging from 0.40 mm up to 0.50 mm. These fiber tapers were used as the main section of the monitoring device, and when chemically treated as immunosensors for the detection of bacteria, yeast and erythrocytes.

On-line detection system of Escherichia coli O157:H7: A new support for Public Health / Sistema de detección en línea de Eschericha coli O157: H7: Un nuevo apoyo para la Salud Pública

Sistema de detección en línea de Escherichia coli O157: H7: Un nuevo apoyo para la Salud Pública.Introducción. Motivado por los casos innumerables de infecciones de diarrea que frecuentemente ocurren, en los países en vías de desarrollo, se calibró un biosensor a fibra optica con intensidad modulada usando como ejemplar Escherichia coli O157: H7. Material y métodos. La sensibilidad del dispositivo se ha calibrado para las unidades formadoras de colonia (CFU) de 10 a 800 rindiendo un error más pequeño que 11 por ciento.Resultados. Se obtuvo una correlación entre la respuesta óptica y el número instantáneo de bacterias. El signo del rendimiento óptico fue 0.016 (ñ 0.001) dB por hora por bacteria. En todo los casos la detección empezó después de 270 ñ 4 minutos, 5-10 veces más rápido que las técnicas bacteriológicas convencionales.Discusión. Los resultados obtenidos pueden contribuir al problema de monitoreo de patógenos que representen problemas en el campo de la salud pública.

Avaliação de mecânica ventilatória por oscilações forçadas: fundamentos e aplicações clínicas / Analysis of the ventilatory mechanics by forced oscillations technique: main concepts and clinical applications

Requerendo apenas cooperação passiva e fornecendo novos parâmetros para análise da mecânica ventilatória, a técnica de oscilações forçadas (TOF) apresenta características complementares aos métodos clássicos de avaliação pulmonar. Neste trabalho, inicialmente é apresentada uma revisão dos princípios da técnica juntamente com uma discussão sobre suas vantagens e atuais limitações. A performance da técnica é comparada com a dos métodos clássicos na detecção de afecções respiratórias. As principais aplicações clínicas reportadas anteriormente na literatura, incluindo a avaliação da mecânica ventilatória infantil, estudos em neonatos, monitorização de pacientes sob ventilação mecânica, medicina ocupacional e avaliação de distúrbios no sono, são revisadas e discutidas. Com base na revisão efetuada e nos resultados obtidos em estudos efetuados em laboratório, os autores concluem que a TOF pode contribuir para um exame mais detalhado, assim como para facilitar a realização de testes de função pulmonar em condições nas quais as técnicas tradicionais não são adequadas.

Implantaçäo de um laboratório de ensaio de conformidade de instrumentos médico-hospitalares / Implantation of laboratory assay conformity of medico hospitalar instruments

A inexistência de certificação de conformidade de produtos médico-hospitalares no Brasil tem contribuido para proliferação de equipamentos sem qualidade e segurança. Entretanto a portaria 2.043 de 12 dezembro de 1994 do Ministário da Saúde institui uma política para garantir a qualidade dos produtos médico-hospitalares. Recentemente o Programa de Engenharia Biomédica iniciou processo de credenciamento de seu laboratório de Instrumentação para ensaios segundo a norma NBR IEC 601-1. Com a experiência adquirida neste processo este trabalho foi desenvolvido. Ele objetiva orientar os laboratórios da área de engenharia biomédica de como implantar um laboratório de certificação de conformidade dde equipamentos médico-hospitalares. Tal laboratório é o instrumento legal para avaliar a qualidade destes produtos

Abstract- ln Brazil, the Jack of conformity's certification of hospital products have contributed to the proliferation of equipment without quality and security. However the law number 2.043 of 12th December, 1994 from the Health's Ministry established a policy to guarantee the quality of hospital products. Recently the Biomedical Engineering Program stm1ed a processes to certificate its Instrumentation Laboratory for tests of conformity under the NBR IEC 601-1 standard. With the experience acquired in this process, this paper has been made. lt has the objective to guide the establishment of a test Jaboratory in the biomedical engineering area. This Jaboratory is the legal way to test and verify the quality ofthese products

Análise da proposta em frequência de sistemas de medida de pressäo empregados na área de fisiologia respiratória / Proposal analysis in frequency of pressure measurements systems used in respiratory physiology area

Neste trabalho, enfocamos brevemente os princípios de operação dos sistemas de medida de pressão e descrevemos um modelo elétrico para o mesmo. Reportamos os resultados da avaliação da resposta em frequência utilizando o transdutor diferencial Honneywell 163PC e diferentes tubos de conexão. Por fim, efetuamos uma discussão dos resultados com base no modelo proposto e nos resultados previamente reportados.

Abstract - ln this work, we made. a brief review of the pressure measurements systems operation principles and described an analog electrical model for these systems. The results of the frequency response using Honeywell 163 PC differential pressure transducer with different tubes are described. Finally, we made a discussion of the results based in the used model and in the previously reported results