Evidence that human Chlamydia pneumoniae was zoonotically acquired.

Zoonotic infections are a growing threat to global health. Chlamydia pneumoniae is a major human pathogen that is widespread in human populations, causing acute respiratory disease, and has been associated with chronic disease. C. pneumoniae was first identified solely in human populations; however, its host range now includes other mammals, marsupials, amphibians, and reptiles. Australian koalas (Phascolarctos cinereus) are widely infected with two species of Chlamydia, C. pecorum and C. pneumoniae. Transmission of C. pneumoniae between animals and humans has not been reported; however, two other chlamydial species, C. psittaci and C. abortus, are known zoonotic pathogens. We have sequenced the 1,241,024-bp chromosome and a 7.5-kb cryptic chlamydial plasmid of the koala strain of C. pneumoniae (LPCoLN) using the whole-genome shotgun method. Comparative genomic analysis, including pseudogene and single-nucleotide polymorphism (SNP) distribution, and phylogenetic analysis of conserved genes and SNPs against the human isolates of C. pneumoniae show that the LPCoLN isolate is basal to human isolates. Thus, we propose based on compelling genomic and phylogenetic evidence that humans were originally infected zoonotically by an animal isolate(s) of C. pneumoniae which adapted to humans primarily through the processes of gene decay and plasmid loss, to the point where the animal reservoir is no longer required for transmission.

Whole genome comparison of the A. fumigatus family.

The availability of the genome sequences of multiple Aspergillus spp. presents the research community with an unprecedented opportunity for discovery. The genomes of Neosartorya fischeri and Aspergillus clavatus have been sequenced in order to extend our knowledge of Aspergillus fumigatus, the primary cause of invasive aspergillosis. Through comparative genomic analysis, we hope to elucidate both obvious and subtle differences between genomes, developing new hypotheses that can be tested in the laboratory. A preliminary examination of the genomes and their predicted proteomes reveals extensive conservation between protein sequences and significant synteny, or conserved gene order. Comparative genomic analysis at the level of these closely related aspergilli should provide important insight into the evolutionary forces at play and their effect on gene content, regulation and expression.

Chromospheric Helium Imaging Photometer (an Instrument for High Time Cadence 1083-nm Wavelength Solar Observations).

The scientific motivation, design criteria, and specifications for a new ground-based instrument to observe the Sun in the He i 1083-nm spectral line is described. The instrument employs a liquid-crystal tunable Lyot-type spectral filter and an array detector that allows the full solar disk to be observed with a time cadence of minutes. We describe the telescope's optical and mechanical features and discuss computer interface and data-reduction procedures employed. Instrument performance during the initial year of operation of the telescope at its high-altitude site is summarized.

Clinical decision making by experienced and inexperienced pediatric physical therapists for children with diplegic cerebral palsy.

BACKGROUND AND PURPOSE: This qualitative study was designed to explore, identify, and describe clinical decision-making processes used by pediatric physical therapists. SUBJECTS: Clinical decision-making processes of three experienced therapists and three inexperienced therapists were assessed as they worked with 18 children with diplegia. METHODS: Retrospective think-aloud procedures were used to elicit verbalizations, which were transcribed, coded, and analyzed. RESULTS: Four characteristics of clinical decision making were identified: (1) Movement scripts provided insights into the clinical application of cognitive schemata based on previous experiences, (2) procedural changes occurred rapidly during within-session decision making, (3) psychosocial sensitivity was important for positive interaction during therapy, and (4) self-monitoring appeared to be pivotal in making clinical decisions as therapists self-assessed their practice. Contrasting data illustrated similarities and differences of experienced and inexperienced clinicians. CONCLUSION AND DISCUSSION: Findings are generally consistent with existing clinical decision-making literature and provide important information for physical therapy practice, research, and education.

Spectrum line intensity as a surrogate for solar irradiance variations.

Active Cavity Radiometer Irradiance Monitor (ACRIM) solar constant measurements from 1980 to 1986 are compared with ground-based, irradiance spectrophotometry of selected Fraunhofer lines. Both data sets were identically sampled and smoothed with an 85-day running mean, and the ACRIM total solar irradiance (S) values were corrected for sunspot blocking (S(c)). The strength of the mid-photospheric manganese 539.4-nanometer line tracks almost perfectly with ACRIM S(e), Other spectral features formed high in the photosphere and chromosphere also track well. These comparisons independently confirm the variability in the ACRIM S(e), signal, indicate that the source of irradiance is faculae, and indicate that ACRIM S(e), follows the 11-year activity cycle.

The behavior equivalence problem in within-subject treatment comparisons.

Within-subject comparisons of multiple treatment effects raise a variety of issues for applied researchers. They include potential nonreversibility of behaviors, practice or habituation effects resulting from repeated presentations of the same stimulus, and the possibility of multiple-treatment interference. It has recently been suggested that the use of item cohorts with equivalent behavioral difficulty addresses those problems. In order to meet the needs of researchers whose primary interest is in domestic, vocational, or other nonacademic skills, a procedure is described for estimating equivalent difficulty for different vocational preparation tasks.