RESUMO
Interleukin-1 (IL-1) is a dimorphic cytokine that acts on target cells through high-affinity receptors, type I and type II. It has been implicated in the onset of term and preterm labour with associated intrauterine infection. To define better the potential action of this cytokine in the human fetal membranes and decidua, the objective of this study was to define the type(s) of IL-1 receptors present in the tissues at term, examine the tissue and cellular distribution of the receptor(s) and determine if there were any changes in their expression or distribution with the onset of labour. Tissues were obtained following elective caesarean section (n=12) or normal labour delivery (n=11). Paraffin embedded and frozen sections were examined by immunohistochemistry and in situ hybridization for evidence of the type I and type II receptors and their corresponding mRNAs. In all tissues studied the type I receptor was localized mainly to the decidua and the type II receptor was localized to the decidua and scattered cells in the amnion-chorion mesenchymal layer. In situ hybridization localized type I receptor mRNAs and type II receptor mRNAs to the decidua. The type I and type II receptor protein in the decidua showed a similar pattern of staining as that found for CD-68, a macrophage marker. The pattern of receptor expression and distribution was unrelated to the mode of delivery. No evidence for the presence of the type I or type II receptor or their mRNAs in the amnion epithelial cells or chorion laeve trophoblast was found.
