RESUMO
In rat liver, peroxisome proliferators induce profound changes in the number and protein composition of peroxisomes, which upon subcellular fractionation is reflected in heterogeneity in sedimentation properties of peroxisome populations. In this study we have investigated the time course of induction of the peroxisomal proteins catalase, acyl-CoA oxidase (ACO) and the 70 kDa peroxisomal membrane protein (PMP70) in different subcellular fractions. Rats were fed a di(2-ethylhexyl)phthalate (DEHP) containing diet for 8 days and livers were removed at different time-points, fractionated by differential centrifugation into nuclear, heavy and light mitochondrial, microsomal and soluble fractions, and organelle marker enzymes were measured. Catalase was enriched mainly in the light mitochondrial and soluble fractions, while ACO was enriched in the nuclear fraction (about 30%) and in the soluble fraction. PMP70 was found in all fractions except the soluble fraction. DEHP treatment induced ACO, catalase and PMP70 activity and immunoreactive protein, but the time course and extent of induction was markedly different in the various subcellular fractions. All three proteins were induced more rapidly in the nuclear fraction than in the light mitochondrial or microsomal fractions, with catalase and PMP70 being maximally induced in the nuclear fraction already at 2 days of treatment. Refeeding a normal diet quickly normalized most parameters. These results suggest that induction of a heavy peroxisomal compartment is an early event and that induction of 'small peroxisomes', containing PMP70 and ACO, is a late event. These data are compatible with a model where peroxisomes initially proliferate by growth of a heavy, possibly reticular-like, structure rather than formation of peroxisomes by division of pre-existing organelles into small peroxisomes that subsequently grow. The various peroxisome populations that can be separated by subcellular fractionation may represent peroxisomes at different stages of biogenesis.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Fígado/ultraestrutura , Peroxissomos/ultraestrutura , Frações Subcelulares/ultraestrutura , Acil-CoA Oxidase , Animais , Western Blotting , Catalase/metabolismo , Dietilexilftalato/farmacologia , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Proteínas de Membrana/metabolismo , Oxirredutases/metabolismo , Peroxissomos/enzimologia , Ratos , Ratos Sprague-Dawley , Frações Subcelulares/enzimologiaRESUMO
Several GTPases of the Rab family, known to be regulators of membrane traffic between organelles, have been described and localized to various intracellular compartments. Rab11 has previously been reported to be associated with the pericentriolar recycling compartment, post-Golgi vesicles, and the trans-Golgi network (TGN). We compared the effect of overexpression of wild-type and mutant forms of Rab11 on the different intracellular transport steps in the endocytic/degradative and the biosynthetic/exocytic pathways in HeLa cells. We also studied transport from endosomes to the Golgi apparatus using the Shiga toxin B subunit (STxB) and TGN38 as reporter molecules. Overexpression of both Rab11 wild-type (Rab11wt) and mutants altered the localization of the transferrrin receptor (TfR), internalized Tf, the STxB, and TGN38. In cells overexpressing Rab11wt and in a GTPase-deficient Rab11 mutant (Rab11Q70L), these proteins were found in vesicles showing characteristics of sorting endosomes lacking cellubrevin (Cb). In contrast, they were redistributed into an extended tubular network, together with Cb, in cells overexpressing a dominant negative mutant of Rab11 (Rab11S25N). This tubularized compartment was not accessible to Tf internalized at temperatures <20 degrees C, suggesting that it is of recycling endosomal origin. Overexpression of Rab11wt, Rab11Q70L, and Rab11S25N also inhibited STxB and TGN38 transport from endosomes to the TGN. These results suggest that Rab11 influences endosome to TGN trafficking primarily by regulating membrane distribution inside the early endosomal pathway.
Assuntos
Compartimento Celular , Endossomos/metabolismo , Glicoproteínas , Proteínas de Membrana , Transporte Proteico/fisiologia , Proteínas rab de Ligação ao GTP/metabolismo , Rede trans-Golgi/metabolismo , Células HeLa , Antígenos de Histocompatibilidade Classe II/metabolismo , Humanos , Membranas Intracelulares/metabolismo , Melanoma Experimental , Glicoproteínas de Membrana/metabolismo , Mutação , Proteínas de Neoplasias/metabolismo , Receptor IGF Tipo 2/metabolismo , Receptores da Transferrina/metabolismo , Toxina Shiga/metabolismo , Sialiltransferases/metabolismoRESUMO
The leptin receptor (OBR) and its ligand leptin (OB) are key players in the regulation of body weight. The OBR is a member of the class I cytokine receptor family and is alternatively spliced into at least six different isoforms. The multiple forms are identical in their extracellular and transmembrane regions but differ in lengths. The two predominant isoforms include a long form (OBR(l)) with an intracellular domain of 303 amino acids and a shorter form (OBR(s)) with an intracellular domain of 34 amino acids. We have constructed a recombinant OBR(l) chimera with the green fluorescent protein (GFP) by fusing GFP to the C-terminus of the OBR(l). The OBR(l)-GFP chimera was transiently transfected and expressed in SHSY5Y and HEK293 cells. In a STAT-Luciferase assay we show that the GFP moiety in this chimera did not affect the signalling capacity of OBR(l)-GFP. In both SHSY5Y and HEK293 cells transfected with OBR(l)-GFP, a predominant intracellular green OBR(l)-GFP fluorescence was detected in vesicles also positive for internalized fluorophore conjugated leptin. We also found that treatment with the lysosomotropic reagent monensin did not relocalize OBR(l)-GFP together with the human transferrin receptor in recycling endosomes, indicating OBR(l)-GFP not to participate in this pathway. In biotinylation-streptavidin pulse chase experiments, using antibodies raised against GFP and OBR, we observed that the rate of early appearance of OBR(s) at the cell surface, upon leptin stimulation, was faster than that found for OBR(l)-GFP. Taken together, our results provide novel data concerning the intracellular trafficking of the two different isoforms of the leptin receptor.
Assuntos
Proteínas de Transporte/biossíntese , Proteínas Luminescentes/química , Proteínas de Membrana/biossíntese , Receptores de Superfície Celular , Biotinilação , Proteínas de Transporte/química , Linhagem Celular , Corantes Fluorescentes , Proteínas de Fluorescência Verde , Humanos , Proteínas de Membrana/química , Monensin/farmacologia , Isoformas de Proteínas/biossíntese , Isoformas de Proteínas/química , Receptores para Leptina , Proteínas Recombinantes de Fusão/química , SolubilidadeRESUMO
Leptin is produced by adipose tissue, and its concentration in plasma is related to the amount of fat in the body. The leptin receptor (OBR) is a member of the class I cytokine receptor family and several different isoforms, produced by alternative mRNA splicing are found in many tissues, including the hypothalamus. The two predominant isoforms includes a long form (OBR) with an intracellular domain of 303 amino acids and a shorter form (OBR) with an intracellular domain of 34 amino acids. Since OBR, is mainly expressed in the hypotalamus, it has been suggested to be the main signalling form. The peripheral production of leptin by adipocyte tissue and its effects as a signal of satiety in the central nervous system imply that leptin gains access to regions of the brain regulating in energy balance by crossing the blood-brain barrier. In an attempt to characterize the intracellular transport of leptin, we have followed binding internalization and degradation of leptin in HEK293 cells. We have also monitored the intracellular transport pathway of fluorescent conjugated leptin in HEK293 cells. Phenylarsine oxide, a general inhibitor of endocytosis, as well as incubation at mild hypertonic conditions, prevented the uptake of leptin, confirming a receptor-mediated internalization process. When internalized, 125I-leptin was rapidly accumulated inside the cells and reached a maximum after 10 min. After 70 minutes about 40-50% of total counts in each time point were found in the medium as TCA-soluble material. Leptin sorting, at the level of early endosomes, did not seem to involve recycling endosomes, since FITC-leptin was sorted from Cy3-transferrin containing compartments at 37 degrees C. At 45 minutes of continuos internalization, FITC-leptin appeared mainly accumulated in late endocytic structures colocalizing with internalized rhodamine coupled epidermial growth factor (EGF) and the lysosomal marker protein lamp-1. The transport of leptin was also shown to engage a monensin and bafilomycin sensitive degradation process in lysosomes. Together, our results provide novel data concerning the uptake, intracellular localization and transport of leptin.
Assuntos
Leptina/metabolismo , Macrolídeos , Antibacterianos/metabolismo , Antibacterianos/farmacologia , Linhagem Celular , Endossomos/metabolismo , Fluoresceína-5-Isotiocianato , Técnica Indireta de Fluorescência para Anticorpo , Corantes Fluorescentes , Humanos , Líquido Intracelular/metabolismo , Ionóforos/metabolismo , Ionóforos/farmacologia , Monensin/metabolismo , Monensin/farmacologia , ATPases Translocadoras de Prótons/antagonistas & inibidoresRESUMO
Recently it has been shown that peroxisomes interact with microtubules which affect the structure and intracellular distribution of the organelle (Schrader, M., J. K. Burkhardt, E. Baumgart, G. Lüers, H. Spring, A. Völkl, H. D. Fahimi: Interaction of microtubules with peroxisomes. Tubular and spherical peroxisomes in HepG2 cells and their alterations induced by microtubule-active drugs. Eur. J. Cell Biol. 69, 24-35 (1996)). In the present work, we have applied immunological techniques to study the organization of peroxisomes within the rat liver cell. Antibodies to a pentadecapeptide corresponding to amino acid residues 403-417 of the 70 kDa integral peroxisomal membrane protein (Kamijo, K., S. Taketani, S. Yokota, T. Osumi, T. Hashimoto: The 70-kDa peroxisomal membrane protein is a member of the Mdr (P-glycoprotein)-related ATP-binding protein super family. J. Biol. Chem. 265, 4534-4540 (1990)) were raised in rabbits and affinity purified. This antibody was found to be highly specific for peroxisomes as determined by ELISA and Western blot analysis. Immunoelectron microscopy of tissue sections from rat liver revealed that peroxisomal membranes were labeled with this antibody and, in addition, labeling was found on tubular extensions often connecting peroxisomes. Antibodies to alpha-tubulin were used to locate the microtubular system. Microtubules were often found in close connection to peroxisomes, suggesting interaction between peroxisomes and the cytoskeleton. Double-labeling experiments for the 70 kDa integral peroxisomal membrane protein and alpha-tubulin demonstrated that the tubular structures connecting peroxisomes did not colocalize with microtubules. These results suggest that peroxisomes are organized in reticular structures within rat liver cells and that the structure and localization of these reticuli may be determined by their association to the microtubular network.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Citoesqueleto/fisiologia , Fígado/metabolismo , Proteínas de Membrana/metabolismo , Microcorpos/metabolismo , Sequência de Aminoácidos , Animais , Anticorpos/imunologia , Técnicas Imunoenzimáticas , Fígado/citologia , Proteínas de Membrana/síntese química , Proteínas de Membrana/imunologia , Microcorpos/ultraestrutura , Dados de Sequência Molecular , Coelhos , RatosRESUMO
We have investigated the effects of peroxisome proliferators on rat liver long-chain acyl-CoA thioesterase activities. Subcellular fractionations of liver homogenates from control, clofibrate- and di(2-ethylhexyl)phthalate-treated rats confirmed earlier studies which demonstrated that peroxisome-proliferating drugs induce long-chain acyl-CoA thioesterase activity mainly in the mitochondrial and cytosolic fractions. The aim of the present study was to investigate whether the induced activities were due to increases in normally expressed enzymes, or due to induction of novel enzymes. To investigate whether structurally different peroxisome proliferators differentially induced thioesterase activities, we tested the effects of di(2-ethylhexyl)phthalate (a plastisizer) and the hypolipidemic drug clofibrate. For this purpose, we established an analytical size exclusion chromatography method. Chromatography of solubilised mitochondrial matrix proteins showed that the activity in control mitochondria was mainly due to enzymes with molecular masses of about 50 kDa and 35 kDa. The activity in samples prepared from clofibrate- and di(2-ethylhexyl)phthalate-treated rats eluted as proteins of about 40 kDa and 110 kDa. Highly purified peroxisomes contained two peaks of activity, which were not induced, that corresponded to molecular masses of 40 kDa and 80 kDa. The 80-kDa peak was shown to be due to dimerization by addition of glycerol. Chromatography of cytosolic fractions from control rat livers indicated the presence of long-chain acyl-CoA thioesterases with molecular masses of approximately 35 kDa and 125 kDa and a broad peak corresponding to a high-molecular-mass protein. The activity in cytosolic fractions from peroxisome-proliferator-treated rats eluted mainly as peaks corresponding to 40, 110 and 150 kDa. In addition, in the 110-kDa peak, a different degree of induction and different chain-length specificities were caused by clofibrate and di(2-ethylhexyl)phthalate, suggesting that these peroxisome proliferators differentially regulate the cytosolic acyl-CoA thioesterase activities. Western blot analysis showed that enzymes in the 40-kDa peak of the peroxisomal and cytosolic fractions were structurally related, but not identical, to a 40-kDa mitochondrial very-long-chain acyl-CoA thioesterase. Our data show that the increased acyl-CoA thioesterase activities in mitochondria and cytosol were mainly due to induction of acyl-CoA thioesterases which are not, or only weakly, expressed under normal conditions.
Assuntos
Fígado/efeitos dos fármacos , Microcorpos/efeitos dos fármacos , Palmitoil-CoA Hidrolase/efeitos dos fármacos , Animais , Western Blotting , Cromatografia em Gel , Fígado/enzimologia , Masculino , Peso Molecular , Palmitoil-CoA Hidrolase/metabolismo , Ratos , Ratos Sprague-Dawley , Frações Subcelulares/enzimologiaRESUMO
According to current concepts, new peroxisomes are formed by division of pre-existing peroxisomes or by budding from a peroxisomal reticulum. Recent cytochemical and biochemical data indicate that protein content in peroxisomes are heterogenous and that import of newly synthesized proteins may be restricted to certain protein import-competent peroxisomal subcompartments (Yamamoto, K., and Fahimi, H. D. (1987) J. Cell Biol. 105, 713-722; Heinemann, P., and Just, W. W. (1992) FEBS Lett. 300, 179-182; Lüers, G., Hashimoto, T., Fahimi, H. D., and Völkl, A. (1993) J. Cell Biol. 121, 1271-1280). We have observed that substantial amounts of peroxisomal proteins are found together with "microsomes" (100,000 x g pellet) after subcellular fractionation of rat liver homogenates. In this study we have investigated the origin of these peroxisomal proteins by modified gradient centrifugation procedures in Nycodenz and by analysis of enzyme activity distributions, Western blotting, and immunoelectron microscopy. It is concluded that much of this material is confined to novel populations of "peroxisomes." Immunocytochemistry on gradient fractions showed that some vesicles were enriched in acyl-CoA oxidase and peroxisomal multifunctional enzyme ("catalase-negative") whereas others were enriched in catalase and thiolase ("acyl-CoA oxidase-negative"). Double immunolabeling experiments verified the strong heterogeneity in the protein contents of these vesicles and also identified peroxisomes varying in size from about 0.5 microns ("normal peroxisomes") to extremely small vesicles of less than 100 nm in diameter. The possibility that these vesicles may be related to different subcompartments of a larger peroxisomal structure involved in protein import and biogenesis will be discussed.
Assuntos
Fígado/ultraestrutura , Microcorpos/ultraestrutura , Animais , Centrifugação com Gradiente de Concentração , Masculino , Microcorpos/fisiologia , Microscopia Imunoeletrônica , Modelos Biológicos , Ratos , Ratos Sprague-DawleyRESUMO
A common function of peroxisomes in eukaryotic cells is beta-oxidation of fatty acids. In animal cells, beta-oxidation is compartmentalized to peroxisomes and mitochondria. Although regulation of beta-oxidation in mitochondria has been extensively studied, knowledge on its regulation in peroxisomes is still limited. We have considered the possibility that peroxisomes may contain acyl-CoA thioesterases with different substrate specificities that possibly regulate metabolism of different lipids by regulation of substrate availability. In the present study, we have investigated the presence of short-chain and long-chain acyl-CoA thioesterase activities in rat liver peroxisomes. Light-mitochondrial fractions, enriched in peroxisomes, were fractionated by Nycodenz density gradient centrifugation and gradient fractions were analyzed for acyl-CoA thioesterase and marker enzyme distributions. Fractionation of livers from normal rats showed that most of the long-chain acyl-CoA thioesterase activity was localized in microsomes and mitochondria, and only low activity was found in fractions containing peroxisomes. The gradient distribution of propionyl-CoA thioesterase activity showed this activity to be localized mainly in mitochondria and in fractions possibly representing lysosomes, with a small peak of activity in peroxisomal fractions. Di(2-ethylhexyl)phthalate treatment induced the specific propionyl-CoA thioesterase activity approximately threefold in the peak mitochondrial fractions and about onefold in peroxisomal fractions; the activity appeared to be almost exclusively localized to these organelles. The specific activity of myristoyl-CoA thioesterase was induced 1-2-fold in peroxisomal peak fractions and more than 10-fold in the mitochondrial peak fraction, whereas it was unchanged in microsomes. The chain-length specificity of acyl-CoA thioesterase activity in isolated peroxisomes suggests that peroxisomes contain an inducible short-chain thioesterase active on C2-C4 acyl-CoA species (possibly a 'propionyl-CoA' thioesterase). In addition, peroxisomes contain medium-chain to long-chain thioesterase activity, probably due to separate enzymes based on the different chain-length specificities observed in peroxisomes from normal and di(2-ethylhexyl)phthalate-treated rats. A long-chain acyl-CoA thioesterase was partially purified from isolated peroxisomes and found to be active only on fatty-acyl-CoA species longer than octanoyl-CoA. The protein is apparently a monomer of about 40 kDa and clearly different from microsomal long-chain acyl-CoA thioesterase. An induction of this long-chain thioesterase may explain the observed change in chain-length specificity in peroxisomes isolated from normal and di(2-ethylhexyl)phthalate-treated rats. Possible physiological functions of these thioesterases are discussed.
Assuntos
Microcorpos/enzimologia , Mitocôndrias Hepáticas/enzimologia , Palmitoil-CoA Hidrolase/isolamento & purificação , Animais , Fracionamento Celular , Centrifugação com Gradiente de Concentração , Cromatografia de Afinidade , Dietilexilftalato/farmacologia , Eletroforese em Gel de Poliacrilamida , Masculino , Mitocôndrias Hepáticas/efeitos dos fármacos , Palmitoil-CoA Hidrolase/química , Palmitoil-CoA Hidrolase/metabolismo , Ratos , Ratos Sprague-Dawley , Especificidade por SubstratoRESUMO
In order to monitor changes in cytosolic Ca2+ levels, brown-fat cells were incubated with the fluorescent Ca2+-indicator fura-2 and the fluorescence intensity ratio followed. The addition of norepinephrine led to a rapid and persistent increase in the cytosolic Ca2+ level, which was dose-dependent with a maximal effect at about 1 microM. The response was diminished in the absence of extracellular Ca2+ and was inhibited more efficiently by phentolamine and prazosin than by propranolol or yohimbine, indicating alpha 1-adrenergic mediation. Accordingly, selective alpha 1-adrenergic stimulation also increased the cytosolic Ca2+ level. However, selective beta-adrenergic stimulation, as well as the adenylate cyclase activator forskolin, were also able to increase the cytosolic Ca2+ level in these cells to a certain extent. It was concluded that the major part of the increase in cytosolic Ca2+ was mediated, as in other cell types, via alpha 1-adrenergic receptors, but that Ca2+ levels were also positively modulated by a cAMP-mediated process. These observations are discussed in relation to known alpha 1/beta synergisms in brown adipose tissue.