Evaluation of ionic air purifiers for reducing aerosol exposure in confined indoor spaces.

UNLABELLED: Numerous techniques have been developed over the years for reducing aerosol exposure in indoor air environments. Among indoor air purifiers of different types, ionic emitters have gained increasing attention and are presently used for removing dust particles, aeroallergens and airborne microorganisms from indoor air. In this study, five ionic air purifiers (two wearable and three stationary) that produce unipolar air ions were evaluated with respect to their ability to reduce aerosol exposure in confined indoor spaces. The concentration decay of respirable particles of different properties was monitored in real time inside the breathing zone of a human manikin, which was placed in a relatively small (2.6 m3) walk-in chamber during the operation of an ionic air purifier in calm air and under mixing air condition. The particle removal efficiency as a function of particle size was determined using the data collected with a size-selective optical particle counter. The removal efficiency of the more powerful of the two wearable ionic purifiers reached about 50% after 15 min and almost 100% after 1.5 h of continuous operation in the chamber under calm air conditions. In the absence of external ventilation, air mixing, especially vigorous one (900 CFM), enhanced the air cleaning effect. Similar results were obtained when the manikin was placed inside a partial enclosure that simulated an aircraft seating configuration. All three stationary ionic air purifiers tested in this study were found capable of reducing the aerosol concentration in a confined indoor space. The most powerful stationary unit demonstrated an extremely high particle removal efficiency that increased sharply to almost 90% within 5-6 min, reaching about 100% within 10-12 min for all particle sizes (0.3-3 microm) tested in the chamber. For the units of the same emission rate, the data suggest that the ion polarity per se (negative vs. positive) does not affect the performance but the ion emission rate does. The effects of particle size (within the tested range) and properties (NaCl, PSL, Pseudomonas fluorescens bacteria) as well as the effects of the manikin's body temperature and its breathing on the ionic purifier performance were either small or insignificant. The data suggest that the unipolar ionic air purifiers are particularly efficient in reducing aerosol exposure in the breathing zone when used inside confined spaces with a relatively high surface-to-volume ratio. PRACTICAL IMPLICATIONS: Ionic air purifiers have become increasingly popular for removing dust particles, aeroallergens and airborne microorganisms from indoor air in various settings. While the indoor air cleaning effect, resulting from unipolar and bipolar ion emission, has been tested by several investigators, there are still controversial claims (favorable and unfavorable) about the performance of commercially available ionic air purifiers. Among the five tested ionic air purifiers (two wearable and three stationary) producing unipolar air ions, the units with a higher ion emission rate provided higher particle removal efficiency. The ion polarity (negative vs. positive), the particle size (0.3-3 microm) and properties (NaCl, PSL, Pseudomonas fluorescens bacteria), as well as the body temperature and breathing did not considerable affected the ionization-driven particle removal. The data suggest that the unipolar ionic air purifiers are particularly efficient in reducing aerosol exposure in the breathing zone when they are used inside confined spaces with a relatively high surface-to-volume ratio (such as automobile cabins, aircraft seating areas, bathrooms, cellular offices, small residential rooms, and animal confinements). Based on our experiments, we proposed that purifiers with a very high ion emission rate be operated in an intermittent mode if used indoors for extended time periods. As the particles migrate to and deposit on indoor surfaces during the operation of ionic air purifiers, some excessive surface contamination may occur, which introduces the need of periodic cleaning these surfaces.

Comparison of filter bag, cyclonic, and wet dust collection methods in vacuum cleaners.

In this study, methods were developed for comparative evaluation of three primary dust collection methods employed in vacuum cleaners: filter bag, cyclonic, and wet primary dust collection. The dry collectors were evaluated with KCl test aerosols that are commonly used in filter testing. However, these aerosols cannot be used for evaluating wet collectors due to their hygroscopicity. Therefore, the wet collectors were evaluated with nonhygroscopic test particles. Both types of test aerosol indicated similar collection efficiencies in tests with dry collectors. The data show that high initial collection efficiency can be achieved by any one of the three dust collection methods: up to 50% for 0.35 microm particles, and close to 100% for 1.0 microm and larger particles. The degree of dependence of the initial collection efficiency on airflow rate was strongly related to the type and manufacturing of the primary dust collector. Collection efficiency decreased most with decreasing flow rate for the tested wet collectors. The tested cyclonic and wet collectors showed high reentrainment of already collected dust particles. After the filter bag collectors had been loaded with test dust, they also reemitted particles. The degree of reentrainment from filter bags depends on the particulate load and the type of filter material used. Thus, the overall particle emissions performance of a vacuum cleaner depends not only on the dust collection efficiency of the primary collector and other filtration elements employed, but also on the degree of reentrainment of already collected particles.

Particle emission characteristics of filter-equipped vacuum cleaners.

Industrial vacuum cleaners with final high-efficiency particulate air (HEPA) filters traditionally have been used for cleanup operations in which all of the nozzle-entrained dust must be collected with high efficiency, for example, after lead-based paint abatement in homes. In this study household vacuum cleaners ranging from $70 to $650 and an industrial vacuum cleaner costing more than $1400 were evaluated relative to their collection efficiency immediately after installing new primary dust collectors in them. Using newly developed testing technology, some of the low-cost household vacuum cleaners equipped with a final HEPA filter were found to have initial overall filtration efficiencies comparable to those of industrial vacuum cleaners equipped with a final HEPA filter. The household vacuum cleaners equipped with a final HEPA filter efficiently collect about 100% of the dry dust entrained by the nozzle. For extensive cleaning efforts and for vacuum cleaning of wet surfaces, however, industrial vacuum cleaners may have an advantage, including ruggedness and greater loading capacity. The methods and findings of this study are applicable to field evaluations of vacuum cleaners.

Test methods for evaluating the filtration and particulate emission characteristics of vacuum cleaners.

The overall filtration efficiency of a vacuum cleaner traditionally has been tested by placing the vacuum cleaner in a test chamber and measuring aerosol concentrations at the chamber inlet and outlet. The chamber test method was refined and validated in this study. However, this chamber test method shows an overall filtration efficiency of close to 100% for most of the industrial vacuum cleaners and for most of the newly developed household vacuum cleaners of midprice range or higher because all these vacuum cleaners have a high-efficiency particulate air (HEPA) or other highly efficient filter installed at the exhaust. A new test method was therefore developed through which the vacuum cleaner was probed in various internal locations so that the collection efficiency of the individual components could be determined. For example, the aerosol concentration upstream of the final HEPA filter can thus be measured, which permits one to estimate the life expectancy of this expensive component. The probed testing method is particularly suitable for field evaluations of vacuum cleaners because it uses compact, battery-operated optical particle size spectrometers with internal data storage. Both chamber and probed tests gave the same results for the aerosol filtration efficiency. The probed testing method, however, also gives information on the performance of the individual components in a vacuum cleaner. It also can be used to determine the dust pickup efficiency and the degree of reaerosolization of particles collected in the vacuum cleaner.

Pathogenomic mechanisms for particulate matter induction of acute lung injury and inflammation in mice.

To begin identifying genes controlling individual susceptibility to particulate matter, responses of inbred mouse strains exposed to nickel sulfate (NiSO4*) were compared with those of mice exposed to ozone (O3) or polytetrafluoroethylene (PTFE). The A strain was sensitive to NiSO4-induced lung injury (quantified by survival time), the C3H/He (C3) strain and several other strains were intermediate in their responses, and the C57BL/6 (B6) strain was resistant. The strains showed a pattern of response similar to the patterns of response to O3 and PTFE. The phenotype of A x B6 offspring (B6AF1) resembled that of the resistant B6 parental strain, with strains exhibiting sensitivity in the order A > C3 > B6 = B6AF1. Pathology was comparable for the A and B6 mice, and exposure to NiSO4 at 15 microg/m3 produced 20% mortality in A mice. Strain sensitivity for the presence of protein or neutrophils in lavage fluid differed from strain sensitivity for survival time, suggesting that they are not causally linked but are controlled by an independent gene or genes. In the B6 strain, exposure to nickel oxide (NiO) by instillation (40 to 1000 nm) or inhalation (50 nm) produced no changes, whereas inhalation of NiSO4 (60 or 250 nm) increased lavage proteins and neutrophils. Complementary DNA (cDNA) microarray analysis with 8,734 sequence-verified clones revealed a temporal pattern of increased oxidative stress, extracellular matrix repair, cell proliferation, and hypoxia, followed by a decrease in surfactant-associated proteins (SPs). Certain expressed sequence tags (ESTs), clustered with known genes, suggest possible coregulation and novel roles in pulmonary injury. Finally, locus number estimation (Wright equation) and a genomewide analysis suggested 5 genes could explain the survival time and identified significant linkage for a quantitative trait locus (QTL) on chromosome 6, Aliq4 (acute lung injury QTL4). Haplotype analysis identified an allelic combination of 5 QTLs that could explain the difference in sensitivity to acute lung injury between parental strains. Positional candidate genes for Aliq4 include aquaporin-1 (Aqp1), SP-B, and transforming growth factor-alpha (TGF-alpha). Transgenic mice expressing TGF-alpha were rescued from NiSO4 injury (that is, they had diminished SP-B loss and increased survival time). These findings suggest that NiSO4-induced acute lung injury is a complex trait controlled by at least 5 genes (all possibly involved in cell proliferation and surfactant function). Future assessment of these susceptibility genes (including evaluations of human synteny and function) could provide valuable insights into individual susceptibility to the adverse effects of particulate matter.

Release of lead-containing particles from a wall enclosure.

The 1995 Department of Housing and Urban Development (HUD) Guidelines for the Evaluation and Control of Lead-Based Paint Hazards in Housing discusses using interior and exterior wall enclosures for lead hazard control. Leaded dust may be aerosolized inside enclosures and released through gaps and cracks into a room. The effects of airflow and mechanical disturbances on dust release were studied using a laboratory wall enclosure model with dust collected from homes with lead-based paint hazards. Airflows relevant to residences were blown down the enclosure and out a 4-, 6-, or 8-mm horizontal gap at its bottom, simulating potential enclosure failure. Then, low-frequency mechanical vibrations also were applied to the enclosure. No significant dust release was found when blowing air down the enclosure even at 37 cm/sec (representing extremely high flow); release occurred only with this high flow and 3 Hz mechanical disturbances. Dust was released primarily from the floor area immediately adjacent to the enclosure gap; the release rate fluctuated over time. Most dust initially settled near the enclosure. Dust release for 1 hour at extreme conditions (high airflow with vibration) yields lead loading above the 1995 HUD clearance level of 100 microg/ft2 only within 3-4 cm of the wall; for the HUD standard (1 ft2) sampling area, the lead loading does not exceed 30 microg/ ft2. Redistributing dust over the room's 16 m2 floor space yields average extreme-condition loading rate of 2 microg/ft2/hour. At less-than-extreme conditions, dust would have to be released for years without cleaning to yield a hazard.

Metal exposure among abrasive blasting workers at four U.S. Air Force facilities.

Button Aerosol Samplers were used to monitor the personal exposure of workers performing abrasive blasting operations at four U.S. Air Force facilities. Inhalable aerosols containing 25 metals, including cadmium, lead, and chromium, were investigated. The Button Aerosol Sampler was chosen because of its ability to successfully withstand mechanical stress, prevent very large particles from collection, and protect the filter from overloading and shredding by rebound particles. In addition, previous studies have shown that the sampling efficiency of this personal Aerosol Sampler exhibits low sensitivity to the ambient air conditions and that it adequately follows the inhalability convention. Inductively coupled plasma (ICP) was used to analyze the collected samples for all 25 metals. In addition, visual absorption spectrophotometry (VAS) was used to analyze for hexavalent chromium because of the presence of strontium chromate. The collected samples yielded 8-hr time-weighted average (TWA) concentrations that were up to 250, 6, and 5 times higher than the permissible exposure limits (PELs) for cadmium, lead, and hexavalent chromium, respectively. Also, the chromium levels measured by the ICP and VAS exceeded the strontium chromate threshold limit value (TLV) by up to 640 and 950 times, respectively. No correlation was found between the ICP and VAS hexavalent chromium concentrations. The likely reasons of this were the presence of Cr (II) and (III) that cannot be detected by the VAS, and the chemical interference from iron and some other metals in the samples. The Button Aerosol Sampler was shown to be useful for the monitoring of workers' exposure to heavy metals during abrasive blasting operations.

Genetic susceptibility to irritant-induced acute lung injury in mice.

Recent studies suggest that genetic variability can influence irritant-induced lung injury and inflammation. To begin identifying genes controlling susceptibility to inhaled irritants, seven inbred mouse strains were continuously exposed to nickel sulfate (NiSO(4)), polytetrafluoroethylene, or ozone (O(3)), and survival time was recorded. The A/J (A) mouse strain was sensitive, the C3H/He (C3) strain was intermediate, and the C57BL/6 (B6) strain was resistant to NiSO(4)-induced acute lung injury. The B6AF(1) offspring were also resistant. The strain sensitivity pattern for NiSO(4) exposure was similar to that of polytetrafluoroethylene or ozone (O(3)). Pulmonary pathology was comparable for A and B6 mice. In the A strain, 15 microg/m(3) of NiSO(4) produced 20% mortality. The strain sensitivity patterns for lavage fluid proteins (B6 > C3 > A) and neutrophils (A >/= B6 > C3) differed from those for acute lung injury. This phenotype discordance suggests that these traits are not causally linked (i.e., controlled by independent arrays of genes). As in acute lung injury, B6C3F(1) offspring exhibited phenotypes (lavage fluid proteins and neutrophils) resembling those of the resistant parental strain. Agreement of acute lung injury strain sensitivity patterns among irritants suggested a common mechanism, possibly oxidative stress, and offspring resistance suggested that sensitivity is inherited as a recessive trait.

Performance characteristics of the button personal inhalable aerosol sampler.

The button inhalable aerosol sampler with a curved porous inlet recently was developed and evaluated as a stationary sampler in the laboratory and in the field. The present study focused on investigating its suitability for personal inhalable aerosol sampling. The button sampler was tested at two wind velocities (0.5 and 2.0 m/sec), three particle sizes (7, 29, and 70 microm) and three orientations to the wind (0, 90, and 180 degrees). The performance characteristics of the button sampler were compared with those of three other personal samplers--the IOM (Institute of Occupational Medicine), GSP, and 37-mm closed-face filter cassette. The experiments were conducted in a wind tunnel with the samplers mounted on a full-size manikin. The direction-specific sampling efficiency of the button sampler was found to be essentially independent of the wind direction and dependent on the wind velocity to a much smaller degree than that of the three other samplers. When direction-averaged, the fit of its sampling efficiency curve to the inhalability curve was found to be better than that of the 37-mm closed-face cassette, comparable with that of the GSP sampler, and less than that of the IOM sampler. The precision of the button sampler was found to be generally equal to or better than the precision of the comparison samplers. It was concluded that the button sampler can be successfully used as a personal inhalable aerosol sampler.

Particle settling after lead-based paint abatement work and clearance waiting period.

This study investigated the evolution of airborne particle concentration and size distribution following abatement work in a controlled environment utilizing direct real-time particle monitoring and used it to project potential lead loadings as those particles settle. An 860 ft3 environmental test chamber with sophisticated ventilation and air purifying systems was built. Wooden doors with lead-based paint were dry sanded or scraped to generate the highest feasible airborne lead concentrations. Size-fractional airborne particle concentrations decreased exponentially with time in all tests, even with no air exchange, consistent with the stirred model of constantly mixed air, which predicts longer settling than for tranquil settling. Very low levels of air mixing generated by temperature gradients and initial room air turbulence affected particle settling. About 90% of airborne lead mass settled within 1 hour after active abatement, before final cleaning began. During the second waiting period of 1 hour, which followed cleaning of the floor, additional dust settled so that the additional potential lead loading from remaining airborne lead was less than 20 microg/ft2. For this worst case scenario, the underestimate of the lead loading done by the clearance sampling did not exceed about 30%. For more realistic conditions, the underestimates are projected to be much lower than the new 40 microg/ft2 Housing and Urban Development (HUD) clearance standards for floor dust lead. These results were obtained for the first waiting period (between the end of active abatement and the beginning of cleaning) of 1 hour, as recommended by HUD guidelines. Thus, this study demonstrates no need to increase either the first or second waiting period.

Performance of Air-O-Cell, Burkard, and Button Samplers for total enumeration of airborne spores.

Performance of three devices used for the total enumeration of airborne spores-the Air-O-Cell sampling cassette, the Burkard personal volumetric air sampler, and the Button Aerosol Sampler--was evaluated under controlled laboratory conditions. The first two are glass-slide impactors; the third collects spores on a filter. The samplers were challenged with 0.44-5.10 microm polystyrene latex particles and five microorganisms of 0.84-3.07 microm mean aerodynamic diameter: Streptomyces albus, Bacillus subtilis, Cladosporium cladosporioides, Penicillium brevicompactum, and Penicillium melinii. An optical particle counter measured the particle concentrations upstream and downstream of each sampler, and thus determined the physical collection efficiency of the three samplers. Collection efficiency of the Button Aerosol Sampler was close to 100% for the entire particle size range studied. The cut-off size of each impactor was 2.3-2.4 microm. Acridine orange (with epifluorescent microscopy) and lactophenol cotton blue (with bright light microscopy) staining techniques were used for the microscopic enumeration of spores. No significant difference in microscopic counts was found (at the 95% significance level) when using these two techniques with the Button Aerosol Sampler filters. When the lactophenol cotton blue staining was used to compare total microbial counts yielded by all three samplers, the Button Sampler showed significantly higher counts for the smaller size microorganisms (B. subtilis and C. cladosporioides). For the larger microorganisms (P. brevicompactum and P. melinii) all three samplers yielded similar results. Uniformity of particle deposition on the collection surface was highest for the Button Aerosol Sampler due to the design of its inlet. Thus, the filter collection method used with the Button Aerosol Sampler is suitable and can be advantageous for the enumeration of total airborne spores.

Survival of mycobacteria on N95 personal respirators.

OBJECTIVES: The overall aim of this study was to investigate the survival and possible growth of Mycobacterium tuberculosis simulant bacteria on respirator filters. METHODS: Mycobacterium smegmatis was used as a biochemical simulant for M tuberculosis. Bacterial survival was tested on National Institute for Occupational Safety and Health-certified N95 respirators from three manufacturers. The first experiments simulated one-time respirator use and subsequent storage for 1 to 9 days under ideal conditions for the growth of mycobacteria: 37 degrees C and 85% relative humidity. The bacteria were loaded on the respirator filters under three different nutritional conditions: in the absence of nutrients; in the presence of human saliva (simulating conditions when the respirator is worn); and in the presence of nutrient broth (for ideal growth potential). The subsequent experiments simulated respirator wear for 2 hours under medium work-load conditions at a breathing rate of 56 L/min. RESULTS: It was found that M smegmatis did not grow on the tested respirators, even when the respirators were stored at temperature, humidity, and nutrition conditions most favorable for microbial growth. However, these bacteria could survive on respirators for up to 3 days during storage. The culturability of M smegmatis was not affected by airflow that simulated the breathing rate associated with medium work-load conditions for 2 hours. CONCLUSIONS: This study shows that M tuberculosis surrogate bacteria collected on a respirator are not able to grow and are able to survive only in ideal (ie, not clinically relevant) conditions. Based on these experiments, we conclude that M tuberculosis is unlikely ever to become an infectious problem in the air again, once it is removed by a respirator.

Analysis of airborne actinomycete spores with fluorogenic substrates.

The reactions between seven fluorogenic substrates and different groups of enzymes, esterases, lipases, phosphatases, and dehydrogenases, were studied in a search for a new method for the detection of actinomycete spores. Fluorescence measurement was chosen as a fast and sensitive method for microbial analysis. The focus of the research was on the spores of important air contaminants: Streptomyces albus and Thermoactinomyces vulgaris. For the measurement of the enzymatic activity, the chosen fluorogenic substrate was added to a mixture of spores and nutrient media, and the resulting fluorescence was measured with a spectrofluorometer. Fluorogenic substrates were found to show enzymatic activities even for dormant spores. Comparison of the enzymatic activities of dormant spores with those of vegetative cells showed similarity of the enzymatic profiles but higher activity for vegetative cells. The increase of enzymatic activity from dormant spores to vegetative cells was not linear but fluctuating. The largest fluctuations were found after 4 to 5 h of incubation. The enzymatic activities of S. albus were 10 to 50 times lower than those of T. vulgaris, except for the dehydrogenase activity, which was seven times higher. These results indicate that analysis with fluorogenic substrates has the potential for becoming a fast and sensitive method for the enumeration and identification of airborne actinomycete spores.

Characteristics of airborne actinomycete spores.

Airborne actinomycete spores, important contaminants in occupational and residential environments, were studied with respect to their (i) release into the air, (ii) aerodynamic and physical size while airborne, and (iii) survival after collection onto agar with an impactor. Three actinomycete species were selected for the tests to exemplify the three main spore types: Streptomyces albus for arthrospores, Micromonospora halophytica for aleuriospores, and Thermoactinomyces vulgaris for endospores. The results show that the incubation conditions (temperature, time, and nutrients) needed for the development of spores for their release into air are different from the conditions that are needed for colony growth only. Additional drying of M. halophytica and T. vulgaris cultures was needed before spores could be released from the culture. The aerodynamic sizes of the spores, measured with an aerodynamic particle sizer, ranged from 0.57 (T. vulgaris) to 1.28 micron (M. halophytica). The physical sizes of the spores, when measured with a microscope and an image analysis system, were found to be smaller than previously reported in the literature. The relative recovery of the spores on agar media ranged from 0.5 (T. vulgaris) to 35% (S. albus). The results indicate that the culturability of the collected airborne actinomycete spores varies widely and is affected by several variables, such as the species and the sampling flow rate. Therefore, alternatives to commonly used cultivation methods need to be developed for the enumeration of actinomycete spores.

Method for measuring the spatial variability of aerosol penetration through respirator filters.

Fibrous filter media are widely used in respirators to remove airborne particulate matter from the inhaled airflow of workers. The N95 half-mask particulate respirator appears to be the most frequently used respirator under the new NIOSH regulation, 42 CFR 84. Considerable spatial variability in light penetration through the fibrous filter medium of an N95 respirator can be seen by visual observation when it is held to the light. This variability is due to the way in which the fibers are manufactured and laid down to form the filter medium. Similar spatial variability is expected in the aerosol penetration through the filters. Therefore, a test method has been developed for measuring the spatial variability in aerosol penetration. The main components of this method are an aerosol generator, a filter test stand with a movable sampling inlet, an aerosol size spectrometer, and an aerosol photometer. Measurements with the filter media of N95 respirators, tested at average filtration velocities corresponding to light, moderate, and heavy work loads, have shown spatial variations in aerosol penetration in excess of 100% relative to the average aerosol penetration for the entire respirator. N95 respirators are required to be at least 95% efficient (i.e., less than 5% penetrating) at the most penetrating particle size, when tested at 85 L/min. Tests with the new method have shown that the aerosol penetration of the most penetrating particles of about 0.1 micron diameter may locally be higher than 5%, while the average aerosol penetration of 0.1 micron particles is less than 5%.

Intranasal angiotensin II directly influences central nervous regulation of blood pressure.

Intranasal administration of some peptides has been shown to directly influence central nervous functions, thus pointing to a nose-brain pathway for these substances in humans. The present study investigated whether intranasal administration of angiotensin II (ANG II) affects central nervous functions of cardiovascular control in a different way from intravenously administered ANG II. In a balanced cross-over design 12 healthy men were treated with ANG II intravenously (2.5 microg), ANG II intranasally (400 microg), and placebo. Angiotensin II, vasopressin, norepinephrine, and epinephrine plasma levels were assessed every 10 min; blood pressure, heart rate, and systemic vascular resistance were measured by a Dinamap, and by continuous, noninvasive body plethysmography. Also, feelings of activation and mood were measured. Intranasal and intravenous administration invoked equivalent increases in plasma levels of ANG II, and induced an acute rise in blood pressure of comparable size and duration. However, subsequent blood pressure profiles differed dependent on intravenous and intranasal ANG II administration; after intravenous ANG II administration blood pressure remained enhanced at an intermediate level, but it returned to normal or even decreased below normal levels after intranasal ANG II administration. Intranasal ANG II also counteracted the decrease in norepinephrine levels observed after intravenous administration of ANG II. Intranasal but not intravenous ANG II enhanced plasma concentrations of vasopressin. This diverging pattern of effects bears similarities with effects of intracerebroventricular administration of ANG II in animals, suggesting that the effects after intranasal administration reflect a direct central nervous action of ANG II.

Tuberculosis control through respirator wear: performance of National Institute for Occupational Safety and Health-regulated respirators.

BACKGROUND: In 1995 the National Institute for Occupational Safety and Health issued new rules for personal respirators. All nine new respirator categories are authorized in health care facilities for the prevention of the transmission of tuberculosis (TB). The new N95 respirator category is the most frequently used for this purpose. Data are presented on their efficiency for collecting TB-size bacteria and their potential for reaerosolizing collected bacteria. METHODS: All measurements of bacterial penetration were performed with dynamic aerosol size spectrometers at flow conditions corresponding to normal wear and respirator certification conditions. The reaerosolization tests were performed at conditions ranging from normal breathing to violent coughing or sneezing. RESULTS: The tested N95 respirators collected 0.1 to 0.3 microm particles with efficiencies of 95% or higher, as specified by the regulations. TB-size bacteria of 0.8 microm and larger, however, were collected with 99.5% or higher efficiencies; that is, the penetration of these bacteria through the filter material was 0.5% or less, much less than the required maximum penetration of 5% for the smaller particle sizes. No bacteria were reaerosolized during normal exhalation. Some reaerosolization (0.1% or less) was observed only at low humidity and extremely high air flow through the respirator, corresponding to violent coughing or sneezing. CONCLUSIONS: The filter materials of N95 respirators provide good protection against TB bacteria. Thus, a significant number of bacteria can enter the respirator-wearer's breathing space only through spaces where the respirator inadequately seals to the wearer's face. Reentrainment and reaerosolization of mycobacteria is not a problem when normal work practices are observed in health care facilities.

Performance of N95 respirators: filtration efficiency for airborne microbial and inert particles.

In 1995 the National Institute for Occupational Safety and Health issued new regulations for nonpowered particulate respirators (42 CFR Part 84). A new filter certification system also was created. Among the new particulate respirators that have entered the market, the N95 respirator is the most commonly used in industrial and health care environments. The filtration efficiencies of unloaded N95 particulate respirators have been compared with those of dust/mist (DM) and dust/fume/mist (DFM) respirators certified under the former regulations (30 CFR Part 11). Through laboratory tests with NaCl certification aerosols and measurements with particle-size spectrometers, N95 respirators were found to have higher filtration efficiencies than DM and DFM respirators and noncertified surgical masks. N95 respirators made by different companies were found to have different filtration efficiencies for the most penetrating particle size (0.1 to 0.3 micron), but all were at least 95% efficient at that size for NaCl particles. Above the most penetrating particle size the filtration efficiency increases with size; it reaches approximately 99.5% or higher at about 0.75 micron. Tests with bacteria of size and shape similar to Mycobacterium tuberculosis also showed filtration efficiencies of 99.5% or higher. Experimental data were used to calculate the aerosol mass concentrations inside the respirator when worn in representative work environments. The penetrated mass fractions, in the absence of face leakage, ranged from 0.02% for large particle distributions to 1.8% for submicrometer-size welding fumes. Thus, N95 respirators provide excellent protection against airborne particles when there is a good face seal.

Field testing of new aerosol sampling method with a porous curved surface as inlet.

A new aerosol sampling method, utilizing a porous curved surface as the sampling inlet, has recently been developed. Previous laboratory evaluations of this method have demonstrated its important features, such as low wind sensitivity and good filter collection uniformity. In this study a prototype incorporating the new method was evaluated in the field as a stationary and personal sampling device. The small sampler, utilizing a 25-mm filter is called the button aerosol sampler and was evaluated for collecting total airborne dust and fungal spores. The study was performed in nine poorly maintained inner-city houses during environmental cleanups at different cleanliness levels. The button sampler was used in parallel with the standard 37-mm closed-face filter cassette. Four collocated samplers of each type were tested at all sites as stationary samplers, and three samplers of each type were tested at two sites as personal samplers. Aerosol samples were collected on filters and analyzed using the gravimetric method for total dust and epifluorescence microscopy for fungal spores. The average particle concentration values measured with the button sampler and with the standard sampling cassette were found to correlate well within ranges of 10(1)-10(3) micrograms/m3 for total dust and 10(3)-10(5) spores/m3 for airborne fungi. The measurement results obtained with the new button sampler showed lower intersample variations of the measured concentration levels and higher uniformity of the particle deposits on the filters than those obtained with the standard cassette.