Optically coherent image formation and denoising using a plug and play inversion framework.

The performance of optically coherent imaging systems can be limited by measurement and speckle noise. In this paper, we develop an image formation framework for computing the maximum a posteriori estimate of an object's reflectivity when imaged using coherent illumination and detection. The proposed approach allows for the use of Gaussian denoising algorithms (GDAs), without modification, to mitigate the exponentially distributed and signal-dependent noise that occurs in coherent imaging. Several GDAs are compared using both simulated and experimental data. The proposed framework is shown to be robust to noise and significantly reduce reconstruction error compared to the standard inversion technique.

Heme charge-transfer band III is vibronically coupled to the Soret band.

A complete resonance Raman excitation profile of the heme charge-transfer band known as band III is presented. The data obtained throughout the near-infrared region show preresonance with the Q-band, but the data also clearly show the enhancement of a number of modes in the spectral region of band III. Only nontotally symmetric modes are observed to have resonance enhancement in the band III region. The observed resonance enhancements in modes of B(1g) symmetry are compared with the enhancements of those same modes in the excitation profiles of the Q-band of deoxy myoglobin, also presented here for this first time. The Q-band data agree well with the theory of vibronic coupling in metalloporphyrins (Shelnutt, J. A. J. Chem. Phys. 1981, 74, 6644-6657). The strong vibronic coupling of the Q-band of the deoxy form of hemes is discussed in terms of the enhancement of modes with both B(1g) and A(2g) symmetry. The comparison between the Q-band and band III reveals that, consistent with the theory, only modes of B(1g) symmetry are enhanced in the vicinity of band III. These results show that band III is vibronically coupled to the Soret band. The coupling of band III to modes with strong rhombic distortion of the heme macrocycle calls into question the hypothesis that the axial iron out-of-plane displacement is primarily responsible for the structure-dynamics correlations observed in myoglobin.