Distribution of P2X(1) and P2X(3) receptors in the rat and human urinary bladder.

Adenosine 5'-triphosphate (ATP) is known to play a significant role as a neurotransmitter in smooth muscle. There is evidence to show that ATP can cause bladder contractions and may also be involved in the processing of sensory information in the urinary bladder. These effects are likely to be mediated by P2X receptors, namely P2X(1) and P2X(3), respectively. This study set out to investigate their distribution in rat and human urinary bladders. P2X(1) receptor immunoreactivity was found on detrusor muscle fibres and P2X(3) receptor immunoreactivity was found in the urothelium of both species. This is the first demonstration of a non-neuronal localisation for P2X(3) receptors. No clear evidence was found for the presence of P2X(3) receptors on calcitonin gene-related peptide-containing sensory nerves and therefore P2X(3) receptors may not have a direct role in the mediation of sensory responses to ATP in the urinary bladder.

Prospective identification of anomalous origin of left coronary artery from the right sinus of valsalva using transthoracic echocardiography: importance of color Doppler flow mapping.

Four cases are discussed in which anomalous origin of the left coronary artery from the right sinus of Valsalva was identified prospectively by transthoracic echocardiography when the anomalous coronary passed between the great arteries. Color Doppler flow mapping was a critical component in identifying the anomalous coronary, particularly when the proximal course of the left main coronary was intramural.

Anomalous origin of the right coronary artery from the left sinus of valsalva: transthoracic echocardiographic diagnosis.

Anomalous origin of the right coronary artery from the left sinus of Valsalva is a rare congenital defect that can be difficult to diagnose by echocardiography. We describe an infant with a ventricular septal defect that was diagnosed prospectively by transthoracic echocardiography as an anomalous origin of the right coronary artery from the left sinus of Valsalva. Subcostal imaging and Doppler color flow mapping were instrumental in the echocardiographic diagnosis of this unusual coronary abnormality.

Diagnosis of decreased cholinesterase activity by amniocentesis.

We report a case in which amniocentesis, performed in order to exclude a fetal chromosomal abnormality, led to a diagnosis of decreased cholinesterase activity. This is a rare but clinically useful application of the amniotic fluid cholinesterase assay.

Dynamics of rural-urban migration in India: 1960-1981.

"This paper analyzes a multi-sectoral simulation model of the Indian economy designed to isolate the sources of Indian economic growth and urbanization since 1960. The model shares many common traits with other computable general equilibrium (CGE) simulation models, and its underlying framework is neoclassical. The model stresses spatial issues so that it can provide predictions on rural/urban labor demands, and hence on migration flows. The central issue we seek to evaluate is whether a neoclassical development paradigm can explain adequately the somewhat paradoxical patterns of urbanization and economic growth observed in India since 1960. Our conclusion is a qualified, affirmative response, based on the model's ability to replicate key macroeconomic variables."

A regimen for obtaining mature human oocytes from donors for research into human fertilization in vitro.

Patients awaiting laparoscopic sterilization were canvassed to allow oocytes to be aspirated from their ovaries at the time of operation for the purpose of testing the fertilizing capacity of spermatozoa from clinically infertile men. Because these patients were volunteers, the standard techniques for stimulation, ovulation prediction, operation, and oocyte recovery had to be modified for maximum safety and for convenience to both patient and staff. Superovulation was induced with 100 mg or 150 mg clomiphene citrate on days 2 to 6 or 5 to 9 of the menstrual cycle. Final maturation of oocytes was stimulated with 5000 IU of human chorionic gonadotropin administered between days 11 and 15 of the cycle, and the operation was performed 34 to 36 hours later on the routine gynecology operation lists. Of the 157 patients canvassed , 47% were willing to donate oocytes to the project. Despite the variability of the stimulation regimens, the simplified operative technique, and the fact that more than half the patients were on oral contraceptives in the previous cycle, one or more oocytes were retrieved in 75% of the patients. At the initial assessment, 83% of these were considered suitable for in vitro fertilization.

Metabolism of prostaglandin F2alpha within the human uterus in early pregnancy.

The potential for intra-uterine metabolism of prostaglandins in early pregnancy was studied by incubation of prostaglandin F2a (PGF2a) with tissues obtained by termination hysterectomy at 7 and 16 weeks gestation. Enzymes regulating the degradation of prostaglandins were demonstrated in placenta and membranes and to a lesser extent, in myometrium and decidua. It is believed that these enzymes may have a physiological role in maintaining the continuity of pregnancy, and that the high degradation of PGF2a in placenta and membranes may be one of the reasons that, for termination of pregnancy, far higher doses are required intra-amniotically than extra-amniotically.

PIP: In vitro investigation was carried out to test the capacity of various intrauterine tissues to metabolize a PG (prostaglandin). 2 complete uteri obtained by total hysterectomy--1 at 7 weeks and 1 at 16 weeks gestation--were used in the study. The homogenized tissues were incubated with PGF2alpha. Enzymes which regulate the degradation (metabolism) of PGs were demonstrated in placenta and membranes and, to a lesser extent, in myometrium and decidua. Both tissue preparations showed considerable metabolism of the PGF2alpha. In other in vitro experiments, PGE has generally been metabolized faster than PGF. Results of this study indicate that the reason higher levels of PG are needed for intraamniotic than extraamniotic 2nd-trimester pregnancy termination is that there is a need to overcome or compensate for their degradation or metabolism.

Factors influencing the pregnancy and complication rates with human menopausal gonadotrophin therapy.

We report the results obtained with a standard system of gonadotrophin therapy. Seventy-seven consecutive patients were given 322 treatment cycles. Thirty-seven patients (48 per cent) conceived, six of them on two occasions, making 43 pregnancies of which 31.6 per cent were multiple. Five per cent of all treatment cycles were complicated by mild, and 0.62 per cent by severe hyperstimulation. The factors involved in achieving a satisfactory pregnancy rate whilst avoiding complications are discussed. Most complications occurred during the first cycle in which the rise in oestrogen excretion suggested follicular development and human chorionic gonadotrophin (HCG) was given (the "first effective" treatment cycle). In such cycles the risk of hyperstimulation rose sharply when the day 6 urinary total estrogen level was above 150 mug. per 24 hours and the multiple pregnancy rate was increased by a large dose of HCG.