RESUMO
The presence of blood cells in milt of rainbow trout (Oncorhynchus mykiss) collected every week between the middle at the end of the spawning season, either by stripping or by catheterization was investigated. Basic sperm biological and biochemical characteristics were also evaluated. Because milt often becomes contaminated with blood during collection, we also studied the influence of experimental blood contamination on sperm motility and biochemical parameters of seminal plasma. We demonstrated the presence of blood cells (erythrocytes, lymphoid, and phagocytes) in rainbow trout milt collected by both methods. Both sampling period and collection method influenced sperm characteristics, however the relationship between these characteristics and blood cells are not clear at present. A high number of blood cells in milt was found in some samples, possibly due to inflammation, because at the same time we observed bacteria and elevated levels of protein and antiproteinase activity in contaminated samples. Experimental contamination of milt with blood did not influence sperm motility, protein concentration and LDH activity of the 5-day-stored semen. Our study demonstrated that blood cells were present in rainbow trout milt. Blood cells may also appear in milt as a result of bleeding and their elevated levels are present during inflammation.
Assuntos
Contagem de Células Sanguíneas/veterinária , Oncorhynchus mykiss/fisiologia , Sêmen/citologia , Manejo de Espécimes/veterinária , Espermatozoides/fisiologia , Animais , Células Sanguíneas/citologia , Masculino , Oncorhynchus mykiss/sangue , Sêmen/fisiologia , Preservação do Sêmen/veterinária , Manejo de Espécimes/métodos , Contagem de Espermatozoides/veterinária , Motilidade dos EspermatozoidesRESUMO
Carp (Cyprinus carpio L.) fry were exposed for 3 weeks to a sublethal concentration of ammonia (0.102 +/- 0.059 mg NH3/dm3). Ammonia induced considerable changes in the level of free amino acids and unfavourable changes in the blood, i.e. leucopaenia and eosinophy. The observed increase of the concentration of most amino acids in fish intoxicated with NH3 suggests that the process reflects detoxication of ammonia which takes place both in the brain and muscles.