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Cannabidiol (CBD) is a non-psychotomimetic phytocannabinoid derived from Cannabis sativa. It has therapeutic effects in different paradigms of brain injury, acting as a neuroprotectant. As oxidative stress is a primary risk factor for brain damage after neonatal hypoxia, we tested the effect of CBD on oxidative status and non-protein-bound iron accumulation in the immature brain after hypoxia. Moreover, we tested whether cannabidiol affects the accumulation of hypoxia-inducible factor-1 alpha (HIF-1α) which plays a key role in the regulation of cellular adaptation to hypoxia and oxidative stress. We used 7-day-old mice randomly assigned to hypoxic or control groups. Immediately after hypoxia or control exposure, pups were randomly assigned to a vehicle or CBD treatment. 24 h later, they were decapitated and the brains were immediately removed and stored for further biochemical analyses. We found that CBD reduced lipid peroxidation and prevented antioxidant depletion. For the first time, we also demonstrated that CBD upregulated HIF-1α protein level. This study indicates that CBD may effective agent in attenuating the detrimental consequences of perinatal asphyxia.
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Canabidiol , Modelos Animais de Doenças , Subunidade alfa do Fator 1 Induzível por Hipóxia , Estresse Oxidativo , Animais , Canabidiol/farmacologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Camundongos , Hipóxia/metabolismo , Hipóxia/tratamento farmacológico , Peroxidação de Lipídeos/efeitos dos fármacos , Antioxidantes/farmacologia , Animais Recém-Nascidos , Encéfalo/metabolismo , Encéfalo/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/uso terapêuticoRESUMO
Phenotypic flexibility is an important source of physiological variation in endotherms and plays an integral role in species' response to rapid environmental changes. Studies of phenotypic flexibility have focused on winter acclimatization and cold endurance, and there are fewer data on summer acclimatization and adjustments in heat dissipation capacity, especially in Temperate-Zone species. We used indirect calorimetry and thermometry to test if thermoregulation at high air temperatures (Ta) varies between spring and summer in four species of European vespertilionid bats: Nyctalus noctula, Pipistrellus nathusii, P. pygmaeus, and P. pipistrellus. We measured subcutaneous body temperature (Tsub), evaporative water loss, and resting metabolic rate while exposing bats to a stepped profile of increasing Ta, from 28 °C-48 °C. We predicted that during summer, bats increase heat tolerance and evaporative cooling capacity, to better tolerate hotter Tas. In contrast, we found lower maximum ratios of evaporative heat loss (EHL) to metabolic heat production (MHP) during summer, but no seasonal differences in maximum Ta tolerated or Tsub. The main cause of this seasonal difference in maximum EHL/MHP seems to be from bats increasing EWL more gradually with increasing Ta in summer than spring, particularly in the smaller Pipistrellus species. Therefore, this seasonal variation in heat-dissipation strategies may reflect enhanced water conservation during summer to avoid dehydration, as bats are confined to roosts for longer and hotter days compared to spring.
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Researchers from diverse disciplines, including organismal and cellular physiology, sports science, human nutrition, evolution and ecology, have sought to understand the causes and consequences of the surprising variation in metabolic rate found among and within individual animals of the same species. Research in this area has been hampered by differences in approach, terminology and methodology, and the context in which measurements are made. Recent advances provide important opportunities to identify and address the key questions in the field. By bringing together researchers from different areas of biology and biomedicine, we describe and evaluate these developments and the insights they could yield, highlighting the need for more standardisation across disciplines. We conclude with a list of important questions that can now be addressed by developing a common conceptual and methodological toolkit for studies on metabolic variation in animals.
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Metabolismo Basal , Animais , Humanos , FenótipoRESUMO
Energy conservation is a clear function of torpor. Although many studies imply that torpor is also a water-saving strategy, the experimental evidence linking water availability with torpor is inconclusive. We tested the relative roles of water and energy shortages in driving torpor, using the Siberian hamster Phodopus sungorus as a model species. To account for the seasonal development of spontaneous heterothermy, we used male hamsters acclimated to short (8L:16D, SP; n = 40) and long (16L:8D, LP; n = 36) photoperiods. We continuously measured body temperature (Tb) during consecutive 32 h of complete removal of water, food, or both, separated by 7.5 d recovery periods. We predicted that all deprivation types would increase the frequency of spontaneous torpor in SP, and induce torpor in LP-acclimated hamsters. Individuals underwent each deprivation type twice in random orders. Food and water deprivation did not induce torpor in LP-acclimated P. sungorus. Patterns of torpor expression varied among deprivation types in SP individuals. Torpor frequency was significantly lower, but bouts were â¼2 h longer and 2.5 °C deeper, during water deprivation compared to food and food-and-water deprivation. Heterothermic responses to all deprivation types were repeatable among individuals. Different torpor patterns during water and food deprivation suggest that water and energy shortages are distinct physiological challenges. Deeper and longer bouts during water deprivation likely led to higher energy and water savings, while shorter and shallower bouts during fasting may reflect a trade-off between energy conservation and food-seeking activity. The lack of a difference between food- and food-and-water-deprived hamsters suggests a higher sensitivity to food than water shortage. This supports the traditional view that energy conservation is the major function of torpor, but suggests that water shortages may also modulate torpor use. The high repeatability of thermoregulatory responses to resource deprivation suggests that these may be heritable traits subject to natural selection.
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Conservação dos Recursos Hídricos , Torpor , Animais , Cricetinae , Privação de Alimentos , Masculino , Phodopus/fisiologia , Fotoperíodo , ÁguaRESUMO
BACKGROUND: The theory of delayed life history effects assumes that phenotype of adult individual results from environmental conditions experienced at birth and as juvenile. In seasonal environments, being born late in the reproductive season affects timing of puberty, body condition, longevity, and fitness. We hypothesized that late-born individuals are more prone to respond to short photoperiod (SP) than early born ones. We used Siberian hamsters Phodopus sungorus, a model species characterized by high polymorphism of winter phenotype. We experimentally distinguished the effect of litter order (first or third) from the effect of exposure to long photoperiod (LP) before winter (3 months or 5 months) by manipulating the duration of LP acclimation in both litters. We predicted that, irrespective of the litter order, individuals exposed to long photoperiod for a short time have less time to gather energy resources and consequently are more prone to developing energy-conserving phenotypes. To assess effect of litter order, duration of acclimation to long days, and phenotype on basal cost of living we measured basal metabolic rate (BMR) of hamsters. RESULTS: Individuals born in third litters had faster growth rates and were bigger than individuals from first litters, but these differences vanished before transfer to SP. Litter order or duration of LP acclimation had no effects on torpor use or seasonal body mass changes, but prolonged acclimation to LP inhibited winter molting both in first and third litters. Moreover, individuals that did not molt had significantly higher BMR in SP than those which molted to white fur. Although one phenotype usually predominated within a litter, littermates were often heterogeneous. We also found that over 10% of individuals presented late response to short photoperiod. CONCLUSIONS: Our data indicate that duration of postnatal exposure to LP may define propensity to photoresponsiveness, regardless of the litter in which animal was born. Existence of littermates presenting different phenotypes suggests a prudent reproductive strategy of investing into offspring of varied phenotypes, that might be favored depending on environmental conditions. This strategy could have evolved in response to living in stochastic environment.
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To maintain constant body temperature (Tb) over a wide range of ambient temperatures (Ta) endothermic animals require large amounts of energy and water. In hot environments, the main threat to endothermic homeotherms is insufficient water to supply that necessary for thermoregulation. We investigated flexible adjustment of traits related to thermoregulation and water conservation during acclimation to hot conditions or restricted water availability, or both, in the zebra finch, Taeniopygia guttata a small arid-zone passerine. Using indirect calorimetry, we measured changes in whole animal metabolic rate (MR), evaporative heat loss (EHL) and Tb before and after acclimation to 23 or 40 °C, with different availability of water. Additionally, we quantified changes in partitioning of EHL into respiratory and cutaneous avenues in birds exposed to 25 and 40 °C. In response to heat and water restriction zebra finches decreased MR, which together with unchanged EHL resulted in increased efficiency of evaporative heat loss. This facilitated more precise Tb regulation in heat-acclimated birds. Acclimation temperature and water availability had no effect on the partitioning of EHL into cutaneous or respiratory avenues. At 25 °C, cutaneous EHL accounted for ~ 60% of total EHL, while at 40 °C, its contribution decreased to ~ 20%. Consistent among-individual differences in MR and EHL suggest that these traits, provided that they are heritable, may be a subject to natural selection. We conclude that phenotypic flexibility in metabolic heat production associated with acclimation to hot, water-scarce conditions is crucial in response to changing environmental conditions, especially in the face of current and predicted climate change.
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Regulação da Temperatura Corporal , Tentilhões , Aclimatação , Animais , Temperatura Alta , TermogêneseRESUMO
Living in a seasonal environment requires periodic changes in animal physiology, morphology and behaviour. Winter phenotype of small mammals living in Temperate and Boreal Zones may differ considerably from summer one in multiple traits that enhance energy conservation or diminish energy loss. However, there is a considerable variation in the development of winter phenotype among individuals in a population and some, representing the non-responding phenotype (non-responders), are insensitive to shortening days and maintain summer phenotype throughout a year. Differences in energy management associated with the development of different winter phenotypes should be accompanied by changes in antioxidant defence capacity, leading to effective protection against oxidative stress resulting from increased heat production in winter. To test it, we analysed correlation of winter phenotypes of Siberian hamsters (Phodopus sungorus) with facultative non-shivering thermogenesis capacity (NST) and oxidative status. We found that in both phenotypes acclimation to winter-like conditions increased NST capacity and improved antioxidant defence resulting in lower oxidative stress (OS) than in summer, and females had always lower OS than males. Although NST capacity did not correlate with the intensity of OS, shortly after NST induction responders had lower OS than non-responders suggesting more effective mechanisms protecting from detrimental effects of reactive oxygen metabolites generated during rewarming from torpor. We suggest that seasonal increase in antioxidant defence is programmed endogenously to predictively prevent oxidative stress in winter. At the same time reactive upregulation of antioxidant defence protects against reactive oxygen species generated during NST itself. It suggests that evolution of winter phenotype with potentially harmful characteristics was counterbalanced by the development of protective mechanisms allowing for the maintenance of phenotypic adjustments to seasonally changing environment.
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Aclimatação/fisiologia , Estresse Oxidativo , Phodopus/fisiologia , Animais , Metabolismo Basal , Feminino , Peróxido de Hidrogênio/sangue , Masculino , Fenótipo , Phodopus/sangue , Estações do Ano , TermogêneseRESUMO
Macrophysiological analyses are useful to predict current and future range limits and improve our understanding of endotherm macroecology, but such analyses too often rely on oversimplifications of endothermic thermoregulatory and energetic physiology, which lessens their applicability. We detail some of the major issues with macrophysiological analyses based on the classic Scholander-Irving model of endotherm energetics in the hope that it will encourage other research teams to more appropriately integrate physiology into macroecological analyses.
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BACKGROUND: Shortening photoperiod triggers seasonal adjustments like cessation of reproduction, molting and heterothermy. However there is a considerable among-individual variation in photoresponsiveness within one population. Although seasonal adjustments are considered beneficial to winter survival, and natural selection should favor the individuals responding to changes in photoperiod (responders), the phenotype non-responding to changes in day length is maintained in population. Assuming the same resource availability for both phenotypes which differ in strategy of winter survival, we hypothesized that they should differ in life history traits. To test this we compared reproductive traits of two extreme phenotypes of Siberian hamster Phodopus sungorus - responding and non-responding to seasonal changes in photoperiod. We bred individuals of the same phenotype and measured time to first parturition, time interval between litters, offspring body mass 3, 10 and 18 days after birth and their growth rate. We also analyzed nest-building behavior. Additionally, we estimated the correlation between reproduction, and basal metabolic rate (BMR) and oxidative status in both phenotypes to infer about the effect of reproductive output on future investments in somatic maintenance. RESULTS: Prior to reproduction responding individuals were smaller than non-responding ones, but this difference disappeared after reproduction. Responding pairs commenced breeding later than non-responding ones but there was no difference in time interval between consecutive litters. Responders delivered smaller offspring than non-responders and more out of responding individuals built the nest during winter than non-responding ones. Reproduction did not affect future investments in somatic maintenance. Phenotypes did not differ in BMR and oxidative status after reproduction. However, concentration of reactive oxygen metabolites (ROM) was highest in responding males, and biological antioxidant potential (BAP) was higher in males of both phenotypes than in females. CONCLUSIONS: Delayed breeding in responding Siberian hamsters and high ROM concentration in male responders support our hypothesis that differences in adjustment to winter result in different life history characteristics which may explain coexistence of both phenotypes in a population. We propose that polymorphism in photoresponsiveness may be beneficial in stochastic environment, where environmental conditions differ between winters. We suggest that non-responding phenotype may be particularly beneficial during mild winter, whereas responders would be favored under harsh conditions. Therefore, none of the phenotypes is impaired when compared to the other.
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The duration of melatonin (MEL) secretion conveys information about day length and initiates a cascade of seasonal phenotypic adjustments in photoresponsive mammals. With shortening days, animals cease reproduction, minimize energy expenditure, enhance thermoregulatory capacity and adjust functioning of the hypothalamic-pituitary-adrenal (HPA) axis to match the winter increase in energy demands. Within each season, stress plays an important role in the flexible adjustments of a phenotype to environmental perturbations. Recent studies have shown that thermal reaction norms of energy metabolism were narrower in winter-acclimated Siberian hamsters, Phodopus sungorus We tested the hypothesis that physiological changes occurring in response to prolonged MEL signals, including changes in the secretion of stress hormones, are responsible for the seasonal decrease in phenotypic flexibility of energy metabolism in photoresponsive mammals. To quantify reaction norms for basal metabolic rate (BMR) and cortisol (CORT) secretion, male Siberian hamsters maintained at a long (16 h:8 h light:dark) photoperiod were acclimated repeatedly for 12â days to 10 and 28°C. As predicted, the phenotypic flexibility of BMR decreased when animals were supplemented with MEL. However, at the same time, mean CORT concentration and the reaction norm for its secretion in response to changes in acclimation temperature increased. These results suggest that decreased sensitivity of HPA axis to CORT signal, rather than changes in CORT level itself, is responsible for the decreased phenotypic flexibility in photoresponsive species. Our results suggest that decreased phenotypic flexibility in winter, together with increased stress hormone secretion, make photosensitive species more vulnerable to climate change.
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Metabolismo Energético , Melatonina/metabolismo , Fenótipo , Phodopus/fisiologia , Fotoperíodo , Aclimatação , Animais , Sistema Hipotálamo-Hipofisário/fisiologia , Masculino , Sistema Hipófise-Suprarrenal/fisiologia , Estações do AnoRESUMO
According to theoretical predictions, endothermic homeotherms can be classified as either thermal specialists or thermal generalists. In high cost environments, thermal specialists are supposed to be more prone to using facultative heterothermy than generalists. We tested this hypothesis at the intraspecific level using male laboratory mice (C57BL/cmdb) fasted under different thermal conditions (20 and 10°C) and for different time periods (12-48â h). We predicted that variability of body temperature (Tb) and time spent with Tb below normothermy would increase with the increase of environmental demands (duration of fasting and cold). To verify the above prediction, we measured Tb and energy expenditure of fasted mice. We did not record torpor bouts but we found that variations in Tb and time spent in hypothermia increased with environmental demands. In response to fasting, mice also decreased their energy expenditure. Moreover, animals that showed more precise thermoregulation when fed had more variable Tb when fasted. We postulate that the prediction of the thermoregulatory generalist-specialist trade-off can be applied at the intraspecific level, offering a valid tool for identifying mechanistic explanations of the differences in animal responses to variations in energy supply.
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Regulação da Temperatura Corporal , Metabolismo Energético , Jejum , Animais , Temperatura Corporal , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Distribuição Aleatória , TemperaturaRESUMO
Basal metabolic rate (BMR) correlates with the cost of life in endothermic animals. It usually differs consistently among individuals in a population, but it may be adjusted in response to predictable or unpredictable changes in the environment. The phenotypic flexibility of BMR is considered an adaptation to living in a stochastic environment; however, whether it is also repeatable it is still unexplored. Assuming that variations in phenotypic flexibility are evolutionarily important, we hypothesized that they are consistently different among individuals. We predicted that not only BMR but also its flexibility in response to changes in ambient temperature (Ta) are repeatable on short- and long-term timescales. To examine this, we acclimated Siberian hamsters (Phodopus sungorus) for 100 d to winterlike and then to summerlike conditions, and after each acclimation we exposed them interchangeably to 10° and 28°C for 14 d. The difference in BMR measured after each exposure defined an individual's phenotypic flexibility (ΔBMR). BMR was repeatable within and among seasons. It was also flexible in both seasons, but in winter this flexibility was lower in individuals responding to seasonal changes than in nonresponding ones. When we accounted for individual responsiveness, the repeatability of ΔBMR was significant in winter (τ = 0.48, P = 0.01) and in summer (τ = 0.55, P = 0.005). Finally, the flexibility of BMR in response to changes in Ta was also repeatable on a long-term timescale, that is, among seasons (τ = 0.31, P = 0.008). Our results indicate the evolutionary importance of the phenotypic flexibility of energy metabolism and suggest that it may be subject to selection.
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Metabolismo Basal/fisiologia , Phodopus/fisiologia , Adaptação Fisiológica , Animais , Regulação da Temperatura Corporal/fisiologia , Cricetinae , Feminino , Masculino , Fenótipo , Estações do Ano , Fatores de TempoRESUMO
As photoperiod shortens with the approach of winter, small mammals should reduce their energy expenditure to survive periods of food limitation. However, within seasons, animals should balance their energy budgets as abiotic conditions change, sometimes unpredictably; cold spells should increase heat production, while warm spells should do the opposite. Therefore, we addressed specific questions about the possible interactions between seasonal acclimatization and the intra-seasonal phenotypic flexibility of metabolic rate. We hypothesized that phenotypic flexibility in small mammals differs seasonally and is greater in summer than in winter, and predicted that seasonal adjustments in energetics, which are driven by photoperiod, overwhelm the influence of variations in the thermal environment. We measured body mass, basal metabolic rate (BMR), facultative non-shivering thermogenesis (fNST), body temperature, and calculated minimum thermal conductance in Siberian hamsters Phodopus sungorus. Animals were acclimated to winter-like, and then to summer-like conditions and, within each season, were exposed twice, for 3 weeks to 10, 20 or 28 °C. We used differences between values measured after these short acclimation periods as a measure of the scope of phenotypic flexibility. After winter acclimation, hamsters were lighter, had lower whole animal BMR, higher fNST than in summer, and developed heterothermy. After these short acclimations to the above-mentioned temperatures, hamsters showed reversible changes in BMR and fNST; however, these traits were less flexible in winter than in summer. We conclude that seasonal acclimation affects hamster responses to intra-seasonal variations in the thermal environment. We argue that understanding seasonal changes in phenotypic flexibility is crucial for predicting the biological consequences of global climate changes.
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Aclimatação/fisiologia , Metabolismo Basal/fisiologia , Regulação da Temperatura Corporal/fisiologia , Phodopus/fisiologia , Animais , Temperatura Corporal , Metabolismo Energético , Masculino , Fenótipo , FotoperíodoRESUMO
An intriguing question is how the capacity of non-shivering thermogenesis (NST)-a special mechanism supporting endothermic thermoregulation in mammals-is affected by selection for high exercise metabolism. It has been proposed that high NST could be a mechanism to compensate for a low basal production of heat. On the other hand, high basal or activity metabolism is associated with physiological characteristics such as high performance of the circulatory system, which are also required for achieving a high NST. Here we tested whether selection for high aerobic exercise performance, which correlates with an increased basal metabolic rate, led to a correlated evolution of maximum and facultative NST. Therefore, we measured the NST of bank voles, Myodes (= Clethrionomys) glareolus, from lines selected for 13-14 generations (n=46) for high aerobic metabolism achieved during swimming and from unselected, control lines (n=46). Open-flow respirometry was used to measure the rate of oxygen consumption (V(·)O2) in anesthetized bank voles injected with noradrenaline (NA). After adjusting for body mass, maximum NST (maximum V(·)O2 recorded after injection of NA) did not differ between the selected (2.38±0.08 mLO2min(-1)) and control lines (2.36±0.08 mLO2min(-1); P=0.891). Facultative NST (= maximum NST minus resting metabolic rate of anesthetized animals) did not differ between the selected (1.49±0.07 mLO2min(-1)) and control lines (1.50±0.07 mLO2min(-1); P=0.985), either. Therefore, our results suggest that NST capacity is not strongly linked to maximum activity-related aerobic metabolic rate.
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Arvicolinae/fisiologia , Metabolismo Energético/fisiologia , Termogênese/fisiologia , Aclimatação/fisiologia , Animais , Metabolismo Basal/fisiologiaRESUMO
Periodic arousals during hibernation consume most of the winter energy budget for hibernating mammals. Evaporative water loss (EWL) is thought to affect the frequency of arousals and thus energy balance, and might have dramatic implications for over-winter survival and fitness. We hypothesized that huddling affects EWL and energy expenditure in torpid mammals. We tested this hypothesis using bats as a model and predicted that, during torpor, EWL and energy expenditure of huddling individuals would be lower than in individuals that are not in a huddle. We measured EWL and metabolic rate of torpid Myotis nattereri (Kuhl, 1817) huddling in groups or roosting individually. Evaporative water loss in huddling individual bats was almost 30% lower than in solitary animals (P=0.03), even after correcting for the effects of metabolic rate. Our results suggest that conservation of water is a substantial benefit underlying huddling by bats during hibernation. Ultimately, huddling could reduce the total cost of hibernation by reducing the number of expensive periodic arousals from torpor caused by the need to supplement water.
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Comportamento Animal/fisiologia , Quirópteros/fisiologia , Hibernação/fisiologia , Perda Insensível de Água/fisiologia , Animais , Metabolismo Basal , Umidade , Modelos Lineares , Masculino , Respiração , TemperaturaRESUMO
During acclimatization to winter, changes in morphology and physiology combined with changes in diet may affect how animals use the nutrients they ingest. To study (a) how thermal acclimation and (b) nutritional history affect the rates at which Siberian hamsters (Phodopus sungorus) oxidize different classes of dietary nutrients, we conducted two trials in which we fed hamsters one of three (13) C-labeled compounds, that is, glucose, leucine, or palmitic acid. We predicted that under acute cold stress (3 hr at 2°C) hamsters previously acclimated to cold temperatures (10°C) for 3 weeks would have higher resting metabolic rate (RMR) and would oxidize a greater proportion of dietary fatty acids than animals acclimated to 21°C. We also investigated how chronic nutritional stress affects how hamsters use dietary nutrients. To examine this, hamsters were fed four different diets (control, low protein, low lipid, and low-glycemic index) for 2 weeks. During cold challenges, hamsters previously acclimated to cold exhibited higher thermal conductance and RMR, and also oxidized more exogenous palmitic acid during the postprandial phase than animals acclimated to 21°C. In the nutritional stress trial, hamsters fed the low protein diet oxidized more exogenous glucose, but not more exogenous palmitic acid than the control group. The use of (13) C-labeled metabolic tracers combined with breath testing demonstrated that both thermal and nutritional history results in significant changes in the extent to which animals oxidize dietary nutrients during the postprandial period.
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Aclimatação/fisiologia , Temperatura Baixa , Metabolismo Energético , Termogênese/fisiologia , Animais , Metabolismo Basal/fisiologia , Peso Corporal , Radioisótopos de Carbono , Cricetinae , Ingestão de Alimentos/fisiologia , Alimentos , Oxirredução , Estações do AnoRESUMO
The concentrations of fatty acids in the diet influence torpor in numerous species of mammals. Much less is known, however, about the potential effects of other types of dietary lipids. One study demonstrated that increasing dietary cholesterol levels during fall feeding increased torpor bout length and also decreased minimum body temperatures during hibernation by chipmunks. Another hibernation study with ground squirrels revealed that the cholesterol contents of both the cerebral cortex and the microsomes were significantly greater during arousal episodes than during torpor bouts, suggesting that cholesterol plays a role in preserving brain function during torpor. We thus predicted that dietary cholesterol enhances daily torpor in mammals as well. We also predicted that the level of cholesterol found in mammalian brain tissues during daily torpor increases with that of the diet. These hypotheses were tested in a series of laboratory feeding and daily torpor experiments involving Siberian hamsters (Phodopus sungorus) maintained on semisynthetic diets varying only in cholesterol content. Hamsters that were maintained on diets with cholesterol contents of 0.3%-2.5% during the summer entered winter daily torpor spontaneously, whereas those that were fed diets that contained no cholesterol did not. This is the first study to demonstrate the effects of a cholesterol-free diet on mammalian torpor. The presence of cholesterol in the summer diet also increased the level of cholesterol found in the brains of hamsters during the winter daily torpor period, but it did not during the summer. These findings support our hypotheses that dietary cholesterol is permissive for daily torpor in mammals and that it also increases brain cholesterol levels during the winter.
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Metabolismo Basal/fisiologia , Colesterol na Dieta/metabolismo , Colesterol/análise , Phodopus/fisiologia , Torpor , Tecido Adiposo/química , Animais , Análise Química do Sangue , Química Encefálica , Cricetinae , Dieta/estatística & dados numéricos , Fígado/química , Fotoperíodo , Estações do AnoRESUMO
Hibernating rodents prior to winter tend to select food rich in polyunsaturated fatty acids (PUFA). Several studies found that such diet may positively affect their winter energy budget by enhancing torpor episodes. However, the effect of composition of dietary fatty acids (FA) on metabolism of normothermic heterotherms is poorly understood. Thus we tested whether diets different in FA composition affect metabolic rate (MR) and the capacity for nonshivering thermogenesis (NST) in normothermic golden hamsters (Mesocricetus auratus). Animals were housed in outdoor enclosures from May 2010 to April 2011 and fed a diet enriched with PUFA (i.e., standard food supplemented weekly with sunflower and flax seeds) or with saturated and monounsaturated fatty acids (SFA/MUFA, standard food supplemented with mealworms). Since diet rich in PUFA results in lower MR in hibernating animals, we predicted that PUFA-rich diet would have similar effect on MR of normothermic hamsters, that is, normothermic hamsters on the PUFA diet would have lower metabolic rate in cold and higher NST capacity than hamsters supplemented with SFA/MUFA. Indeed, in winter resting metabolic rate (RMR) below the lower critical temperature was higher and NST capacity was lower in SFA/MUFA-supplemented animals than in PUFA-supplemented ones. These results suggest that the increased capacity for NST in PUFA-supplemented hamsters enables them lower RMR below the lower critical temperature of the thermoneural zone.
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Metabolismo Basal/efeitos dos fármacos , Gorduras na Dieta/farmacologia , Ácidos Graxos/farmacologia , Mesocricetus/fisiologia , Termogênese/efeitos dos fármacos , Animais , Metabolismo Basal/fisiologia , Comportamento Animal/fisiologia , Índice de Massa Corporal , Temperatura Corporal/fisiologia , Cricetinae , Ácidos Graxos Monoinsaturados/farmacologia , Ácidos Graxos Insaturados/farmacologia , Hibernação/fisiologia , Masculino , Termogênese/fisiologiaRESUMO
We examined the effect of different dietary supplements on seasonal changes in body mass (m(b)), metabolic rate (MR) and nonshivering thermogenesis (NST) capacity in normothermic Siberian hamsters housed under semi-natural conditions. Once a week standard hamster food was supplemented with either sunflower and flax seeds, rich in polyunsaturated fatty acids (FA), or mealworms, rich in saturated and monounsaturated FA. We found that neither of these dietary supplements affected the hamsters' normal winter decrease in m(b) and fat content nor their basal MR or NST capacity. NST capacity of summer-acclimated hamsters was lower than that of winter-acclimated ones. The composition of total body fat reflected the fat composition of the dietary supplements. Resting MR below the lower critical temperature of the hamsters, and their total serum cholesterol concentration were lower in hamsters fed a diet supplemented with mealworms than in hamsters fed a diet supplemented with seeds. These results indicate that in mealworm-fed hamsters energy expenditure in the cold is lower than in animals eating a seed-supplemented diet, and that the degree of FA unsaturation of diet affects energetics of heterotherms, not only during torpor, but also during normothermy.