Morpho-agronomic variability of okra [Abelmoschus esculentus (L.) Moench] genotypes in Dire Dawa, eastern Ethiopia.

A total of 21 okra genotypes were evaluated for 25 morpho-agronomic traits in 2020 at Dire Dawa, Ethiopia in a randomized complete block design with three replications. Analysis of variance showed significant differences at p<0.05 level of significance for all traits. Estimates of genotypic (GCV) and phenotypic (PCV) coefficients of variation range from 9.16 to 42.3% and 9.33 to 44.16%, respectively. Heritability in a broad sense (H2) and genetic advance as a percent of the mean (GAM) ranged from 29.57 to 91.89% and 10.39 to 83.53%, respectively. Estimated variability components (GCV, PCV, H2, and GAM) were high and moderate for all traits except days to 50% emergence 9.33% of GCV and PCV, internode length 9.16% of GCV and green fruit width 29.57% of H2 that were categorized under low. The first four principal component axes (PCA1 to PCA4) accounted for 7.83 to 35.02%, which accounted 74.56% of the total variability with eigenvalues that ranged from 1.95 to 8.75. Genetic distances estimated by Euclidean distance from the 25 traits ranged from 2.33 to 12.56 with a mean of 6.83, standard deviation of 1.8, and a coefficient of variation of 26.46%. The genotypes were grouped into four distinct clusters using the Euclidean distance matrix using UPGMA. Indigenous okra genotypes collected from Ethiopia were more divergent with high genetic distances and had a higher performance for most of the traits including growth, green fruit yield, and seed yield than introduced genotypes. In conclusion, this study showed the presence of variation among genotypes for most of the traits, indicating that selection of genotypes could be effective to develop okra varieties with high green fruit and seed yield through direct selection or crossing.

Effects of preharvest applications of chemicals and storage conditions on the physico-chemical characteristics and shelf life of tomato (Solanum lycopersicum L.) fruit.

The aim of this study was to investigate the influence of preharvest sprays of chemicals and evaporative cooling (ZECC) on the postharvest physico-chemical quality and shelf life of tomatoes. The experiment was conducted using a combination of ten preharvest sprays of chemicals (chitosan (0.1%), chitosan (0.3%), chitosan (0.5%), salicylic acid (SA) (0.015%), SA (0.03%), SA (0.045%), calcium chloride (CaCl2) (1%), CaCl2 (3%), CaCl2 (5%) and control) and two storage conditions (ambient environment storage and ZECC). The design of the experiment was completely randomized design with three replications. Data were collected from tomato samples (Solanum lycopersicum L. cv. ARP tomato D2) on four days interval. The result showed that all physico-chemical quality indicators were significantly (p ≤ 0.05) affected by both preharvest sprays and storage conditions. CaCl2 (5%) sprays extended the shelf life of tomato by 6 days in ZECC and 11 days in ambient storage compared to the control. The highest concentration of CaCl2 and SA sprays minimized PLW and maintained fruit marketability compared to the control. The firmness of tomatoes was better retained with the sprays of SA (0.045%) in both storage conditions. CaCl2 (5%), SA (0.045%) and chitosan (0.5%) sprays retained the titratable acidity by 42.86%, 47.62% and 33.33%, respectively compared to the control inside ZECC storage. At the end of storage period, the highest TSS was observed on tomatoes sprayed with SA (0.03%) followed by chitosan (0.1%) and chitosan (0.3%). The lycopene contents were lower with sprays of SA (0.045%), SA (0.03%), CaCl2 (3%), CaCl2 (5%) and chitosan (0.1%) stored inside ZECC indicating the effectiveness of preharvest sprays in delaying lycopene accumulation. Moreover, the AA contents of tomatoes sprayed with CaCl2 (5%), SA (0.045%) and chitosan (0.1%) were higher by 34.10%, 38.19% and 23.84%, respectively compared to the control. The result indicated that combining preharvest chemical sprays and ZECC storage is important to maintain the physico-chemical quality and shelf life of tomatoes.

Duration of of Low-Temperature Storage, Clove Topping and Gibberellic Acid on Emergence, Yield and Yield Components of Garlic.

Dormancy of freshly harvested garlic cloves does not allow early emergence, and preplanting garlic clove treatment is critical for more than one cycle of production in a year. This field experiment was conducted to assess the effect of treating cloves on emergence, growth, and yield of "Tseday" variety during the main cropping season of 2014/2015 and off-season of 2015/16 at Haramaya University. The experiment was arranged in a factorial combination of four (cold stored at 7°C for the duration of 10, 20, and 30 days and stored at room temperature 21°C for 30 days as control), two (whole and topped clove), and four GA3 treatment at concentration of 0, 125, 250, and 375 mg/l and distilled water treatment as second control laid out in randomized complete block design with three replications. The three main factors (clove type, cold storage duration, and GA3) and growing season showed significant influence on phenology, growth, bulb yield and yield components, and all size categories of cloves, while GA3 showed nonsignificant effect on days to emergence of cloves. Clove type interacted with cold storage duration and GA3, and cold storage duration interacted with GA3 significantly to influence all characters of the variety. In addition, the growing season also interacted with clove type and GA3 to influence all categories of clove size, bulb diameter, average bulb weight, and total bulb yield. The three main factors (clove type × cold storage duration × GA3) interaction significantly influenced all characteristics of the variety. However, 30 days of cold-stored and topped cloves reduced dormancy period and days to maturity by 18.84 and 19.50 days, respectively, and increased total bulb yield by 70.32% as compared to the control treatment. In addition, this treatment combination significantly increased most of the growth and bulb yield components, while the number and weight of small-sized cloves were decreased. Hence, it can be concluded that 30 days of cold storage and topping of cloves without soaking under GA3 could be recommended to enhance early emergence, good vegetative growth, and total bulb yield of the garlic variety both under rain-fed and irrigated conditions.

Effect of packaging materials and storage environment on postharvest quality of papaya fruit.

This experiment was conducted to assess the effects of packaging materials and storage environments on shelf life of papaya fruit (Carica papaya L.). A factorial combination of five packaging materials and two storage environments using randomized complete block design with three replications were used. The papaya fruits were evaluated for weight loss, percentage marketability, firmness, total soluble solids, pH, titratable acidity, ascorbic acid, reducing sugar and total sugar content. The packaged and cooled fruits remained firmer than unpackaged and evaporatively cooled fruits. Higher chemical compositions were recorded in the control fruits stored under ambient conditions during the earlier times of storage. Packaging and cooling maintained the chemical quality of papaya fruits better than the control sample fruits towards the end of storage periods. The evaporatively cooled storage combined with packaging improved the shelf life of papaya fruits by more than two fold. The polyethylene bag packaging combined with evaporatively cooled storage maintained the superior quality of papaya fruit for a period of 21 days. This integrated agro-technology is recommended for postharvest loss reduction biotechnology in hot regions.

Effects of variety on the quality of tomato stored under ambient conditions.

Three processing and six fresh market tomato varieties harvested at "mature green" stage were evaluated for changes in quality. The total soluble solid, titratable acidity, sugar-acid ratio, pH, ascorbic acid content, colour and firmness were assessed. The storage room air temperature and relative humidity varied from 15.4(○)C to 16.2(○)C and 34.8% to 52.4%, respectively. At harvest, Marglobe Improved had better chemical quality characteristics compared with the other five fresh market varieties while towards the end of the storage period, chemical quality characteristics were maintained better in Fetane. The highest pH and sugar/acid ratio were obtained in Metadel compared with all other varieties throughout the storage period. Among the processing tomato variety, Roma VF had better chemical quality than other two processing varieties. Melkashola had the highest total soluble solid content in the group. Although the processing varieties ripened earlier than fresh market ones, they were notably firmer with better quality. The processing types were better in their chemical quality than fresh market genotypes. The fresh table tomato varieties had higher sugar-acid ratio and vitamin C. Marglobe Improved and Metadel could be selected in favor of higher nutritional quality whereas Fetane could be considered for maintenance of better overall quality characteristics.

Postharvest quality and shelf life of some hot pepper varieties.

Five hot pepper varieties were grown at Haramaya University Research Farm, Dire Dawa, Ethiopia. The experiment was undertaken using completely randomized block design (RCBD) with three replications. Green peppers were stored under ambient and evaporatively cooled conditions. Significant (P ≤ 0.05) differences were observed among cultivars and storage conditions in changes in quality of pepper. Storage at ambient conditions resulted in high weight loss and rapid deterioration in chemical composition. The highest and lowest moisture contents were recorded in Melka Eshet and PBC 600, respectively. Evaporative cooler reduced weight loss and maintained higher levels of pH, ascorbic acid and marketability. The highest weight loss was recorded in Melka Dima stored at ambient condition. The lowest weight loss and highest ascorbic acid content was obtained in Mareko Fana stored in evaporative cooler. After 16 days of storage, all pepper fruits stored at ambient condition were unmarketable while those stored in evaporative cooler were kept up to 28 days. The highest pungency level was observed in ambient storage environment than in evaporative cooler. Evaporative cooling was shown to improve shelf life by four-fold compared to the ambient condition. The shelf life of pepper was improved in all varieties tested while the quality characteristics were maintained better in Mareko Fana than in the other varieties when harvested at mature-green stage and stored under evaporatively cooled storage.