RESUMO
Radiotherapy for the nasopharyngeal cancer patient with poor oral care may lead to severe deterioration of the dentition and may require multiple extractions. Although the use of an implant-retained overdenture can successfully restore the function and esthetics of edentulous patients, its use can be complicated by the tissue changes subsequent to head and neck irradiation. The difficulties in implant treatment planning for an edentulous patient with macroglossia and soft tissue changes following radiotherapy are discussed.
Assuntos
Irradiação Craniana/efeitos adversos , Cárie Dentária/etiologia , Implantação Dentária Endóssea , Prótese Dentária Fixada por Implante , Prótese Total Inferior , Hipertrofia Gengival/cirurgia , Arcada Edêntula/reabilitação , Macroglossia/cirurgia , Feminino , Hipertrofia Gengival/complicações , Hipertrofia Gengival/etiologia , Gengivectomia , Humanos , Macroglossia/complicações , Mandíbula , Pessoa de Meia-Idade , Neoplasias Nasofaríngeas/reabilitação , Extração DentáriaRESUMO
The wzy/rfc gene, encoding the O-antigen polymerase, of Salmonella enterica serovar Typhimurium has been previously cloned and sequenced. In the present work, the wzy transcriptional startpoint was initially identified by primer extension. Next, wzy promoter strength in Escherichia coli K-12 was measured, and was found to be greater than that of the induced lac promoter. To define the Wzy translational startpoint, DNA including the wzy promoter and the putative first five residues of the Wzy protein was fused to the N-terminus of glutathione-S-transferase, and the fusion protein purified by affinity chromatography. N-terminal amino acid sequencing yielded the Wzy translational startpoint. Next, the Wzy protein was C-terminally tagged with the FLAG peptide, and immunoblotting of an S. typhimurium strain expressing a low-copy wzy-FLAG gene (five copies per cell) localized the intact Wzy protein in the cytoplasmic membrane of S. typhimurium cells. The Wzy protein was not well-expressed from a multi-copy wzy-FLAG+ plasmid in S. typhimurium, or in E. coli K-12.