RESUMO
Major Depressive Disorder (MDD) is common and disabling, and is linked to functional impairment and increased mortality. While current treatments for MDD are moderately effective, ultimately, up to one third of patients do not achieve full remission. Interestingly, while affective symptoms of major depression typically resolve with the depressive episode, cognitive impairment frequently persists, and has been identified as one of the most prominent predictors of illness recurrence. Additionally, MDD is well-recognised as a key risk factor for further cognitive decline and dementia. Yet, available treatments for MDD do not typically address cognitive impairment. Cognitive training, represents a promising and novel therapeutic intervention in this regard. This review systematically identified and evaluated the evidence for cognitive training in adults with MDD. Following PRISMA guidelines, eligible studies were selected according to pre-defined criteria delineating our target population (adults with clinically defined MDD), parameters for cognitive training interventions (computer-or strategy-based, clinician-facilitated), and study design (controlled trials including pre-post cognitive and psychological or functional outcome data). Of 448 studies identified, nine studies met inclusion criteria. These studies were evaluated for methodological quality and risk of bias. Despite heterogeneity, qualitative and meta-analytic synthesis of study findings revealed significant improvements in cognitive and affective outcomes following cognitive training, with moderate pooled effect sizes. Unfortunately, very few studies investigated 'far transfer' to broader domains of everyday functioning. Overall, given the strong evidence for the efficacy and value of cognitive training in this context, cognitive training should be considered as a primary therapeutic intervention in the treatment of MDD.
Assuntos
Disfunção Cognitiva , Transtorno Depressivo Maior , Adulto , Cognição , Disfunção Cognitiva/etiologia , Disfunção Cognitiva/terapia , Transtorno Depressivo Maior/terapia , Humanos , Projetos de PesquisaRESUMO
RESEARCH IN CONTEXT: Parents of children with life threatening illness or injuries are at elevated risk of distress reactions, involving symptoms of acute stress disorder, depression and anxiety. Currently, the impact of child illness factors is unclear, and to date research systematically examining the prevalence of these psychological reactions across different illness groups with an acute life threat is sparse. This is important to explore given that studies show that parent functioning impacts on the psychological adjustment and recovery of the ill child. WHAT DOES THIS STUDY ADD?: At four weeks following a child's diagnosis of a serious illness, 49-54% of parents met DSM-IV criteria for acute stress disorder, across a number of illness groups, whereas 15-27% of parents were in the moderate/severe range for depression and anxiety, and 25-31% for stress. Results from this study demonstrate that rates and severity of these psychological reactions in parents of seriously ill children do not vary according to illness type. BACKGROUND: A life threatening childhood illness/injury can lead to significant distress reactions in parents, with independent studies finding such reactions in several different illness groups. To date, there is limited research systematically comparing the prevalence of adverse parental psychological reactions across different childhood illness groups with an acute life threat. This study aimed to investigate the frequency and severity of symptoms of acute traumatic stress, depression, anxiety and general stress in parents, following admission of their child to hospital for a life threatening illness. The study also aimed to explore the relationship between these symptoms, and to determine whether they differ according to illness/injury. METHODS: Cross-sectional data from a prospective, longitudinal study are reported. Participants were 194 parents of 145 children (49 couples), admitted to cardiology (n=53), oncology (n=40) and pediatric intensive care units (n=52), for serious illnesses/injuries. Parents completed self-report questionnaires within four weeks of hospital admission. RESULTS: Rates of acute traumatic stress (P=0.262), depression (P=0.525), anxiety (P=0.453) and general stress symptoms (P=0.720) in parents were comparable across illness type, with 49-54% reaching criteria for acute stress disorder, 15-27% having clinical levels of depression and anxiety, and 25-31% for general stress. Anxiety was most strongly associated with acute traumatic stress (r=0.56), closely followed by stress (r=0.52) and depression (r=0.49), with all correlations highly significant (P<0.001). CONCLUSIONS: These findings provide evidence that the child's medical condition is not associated with parents' experience of clinically significant psychological symptoms, and emphasize the importance for health care providers to be aware of these potential psychological reactions in parents, regardless of the type of illness.
Assuntos
Ansiedade/epidemiologia , Estado Terminal/psicologia , Depressão/epidemiologia , Pais/psicologia , Transtornos de Estresse Pós-Traumáticos/epidemiologia , Adulto , Ansiedade/psicologia , Austrália/epidemiologia , Criança , Pré-Escolar , Estudos Transversais , Depressão/psicologia , Feminino , Humanos , Unidades de Terapia Intensiva Pediátrica , Estudos Longitudinais , Masculino , Relações Pais-Filho , Prevalência , Estudos Prospectivos , Transtornos de Estresse Pós-Traumáticos/psicologiaRESUMO
BACKGROUND AND PURPOSE: Selective nociceptor fibre block is achieved by introducing the cell membrane impermeant sodium channel blocker lidocaine N-ethyl bromide (QX-314) through transient receptor potential V1 (TRPV1) channels into nociceptors. We screened local anaesthetics for their capacity to activate TRP channels, and characterized the nerve block obtained by combination with QX-314. EXPERIMENTAL APPROACH: We investigated TRP channel activation in dorsal root ganglion (DRG) neurons by calcium imaging and patch-clamp recordings, and cellular QX-314 uptake by MS. To characterize nerve block, compound action potential (CAP) recordings from isolated nerves and behavioural responses were analysed. KEY RESULTS: Of the 12 compounds tested, bupivacaine was the most potent activator of ruthenium red-sensitive calcium entry in DRG neurons and activated heterologously expressed TRPA1 channels. QX-314 permeated through TRPA1 channels and accumulated intracellularly after activation of these channels. Upon sciatic injections, QX-314 markedly prolonged bupivacaine's nociceptive block and also extended (to a lesser degree) its motor block. Bupivacaine's blockade of C-, but not A-fibre, CAPs in sciatic nerves was extended by co-application of QX-314. Surprisingly, however, this action was the same in wild-type, TRPA1-knockout and TRPV1/TRPA1-double knockout mice, suggesting a TRP-channel independent entry pathway. Consistent with this, high doses of bupivacaine promoted a non-selective, cellular uptake of QX-314. CONCLUSIONS AND IMPLICATIONS: Bupivacaine, combined with QX-314, produced a long-lasting sensory nerve block. This did not require QX-314 permeation through TRPA1, although bupivacaine activated these channels. Regardless of entry pathway, the greatly extended duration of block produced by QX-314 and bupivacaine may be clinically useful.
Assuntos
Anestésicos Locais/farmacologia , Bupivacaína/farmacologia , Lidocaína/análogos & derivados , Bloqueio Nervoso , Bloqueadores dos Canais de Sódio/metabolismo , Anestésicos Locais/administração & dosagem , Animais , Comportamento Animal/efeitos dos fármacos , Bupivacaína/administração & dosagem , Cálcio/metabolismo , Linhagem Celular , Traumatismos do Pé , Gânglios Espinais/efeitos dos fármacos , Gânglios Espinais/metabolismo , Injeções , Lidocaína/metabolismo , Masculino , Camundongos Knockout , Técnicas de Patch-Clamp , Nervos Periféricos/efeitos dos fármacos , Cultura Primária de Células , Ratos , Ratos Sprague-Dawley , Nervo Isquiático/efeitos dos fármacos , Canal de Cátion TRPA1 , Canais de Potencial de Receptor Transitório/genética , Canais de Potencial de Receptor Transitório/metabolismoRESUMO
OBJECTIVES: To evaluate whether augmenting neuronal protective mechanisms might slow or arrest experimental diabetic peripheral neuropathy (DPN). DPN is one of the most common neurodegenerative disorders and is rising in prevalence. How it targets sensory neurons is uncertain; the disorder is irreversible and untreatable. We explored the intrinsic protective properties of overexpressed human HSP27 on experimental DPN. HSP27 is a small pro-survival heat shock protein that also increases axonal regeneration. METHODS: Experimental diabetes was superimposed on mice overexpressing a human HSP27 transgene and its impact was evaluated on epidermal innervation, behavioral tests of sensation and electrophysiological indices of DPN. RESULTS: Mice that overexpress human HSP27 in their sensory and motor neurons and that were made diabetic for 6 months by streptozotocin treatment were protected from a range of neuropathic abnormalities, including loss of footpad thermal sensation, mechanical allodynia, loss of epidermal innervation, and slowing of sensory conduction velocity. The protection was selective for sensory neurons in comparison to motor neurons and at 6 months provided better protection in female than male mice. Markers of RAGE-NFκB activation were attenuated by the transgene. CONCLUSIONS: The findings support the idea that diabetic polyneuropathy involves a unique, sensory-centric neurodegenerative process which can be reduced by overexpressing a single gene, an important starting point for new disease-modifying therapeutic approaches.
Assuntos
Diabetes Mellitus Experimental/patologia , Gânglios Espinais/patologia , Regulação da Expressão Gênica/genética , Proteínas de Choque Térmico HSP27/metabolismo , Células Receptoras Sensoriais/metabolismo , Fatores Etários , Análise de Variância , Animais , Glicemia/efeitos dos fármacos , Glicemia/genética , Caspase 3/metabolismo , Diabetes Mellitus Experimental/induzido quimicamente , Modelos Animais de Doenças , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Hemoglobinas Glicadas/metabolismo , Proteínas de Choque Térmico HSP27/genética , Humanos , Hiperalgesia/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Fibras Nervosas/metabolismo , Fibras Nervosas/patologia , Fibras Nervosas/fisiologia , Condução Nervosa/efeitos dos fármacos , Condução Nervosa/genética , Limiar da Dor/fisiologia , Nervos Periféricos/patologia , Nervos Periféricos/fisiopatologia , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Pele/inervação , Pele/metabolismo , Estreptozocina/farmacologia , Fatores de Tempo , Quinase Induzida por NF-kappaBRESUMO
BACKGROUND AND PURPOSE: We have developed a strategy to target the permanently charged lidocaine derivative lidocaine N-ethyl bromide (QX-314) selectively into nociceptive sensory neurons through the large-pore transient receptor potential cation channel subfamily V (TRPV1) noxious heat detector channel. This involves co-administration of QX-314 and a TRPV1 agonist to produce a long-lasting local analgesia. For potential clinical use we propose using lidocaine as the TRPV1 agonist, because it activates TRPV1 at clinical doses. EXPERIMENTAL APPROACH: We conducted experiments in rats to determine optimal concentrations and ratios of lidocaine and QX-314 that produce the greatest degree and duration of pain-selective block when administered nearby the sciatic nerve: reduction in the response to noxious mechanical (pinch) and to radiant heat stimuli, with minimal disruption in motor function (grip strength). KEY RESULTS: A combination of 0.5% QX-314 and 2% lidocaine produced 1 h of non-selective sensory and motor block followed by >9 h of pain-selective block, where grip strength was unimpaired. QX-314 at this concentration had no effect by itself, while 2% lidocaine by itself produced 1 h of non-selective block. The combination of 0.5% QX-314 and 2% lidocaine was the best of the many tested, in terms of the duration and selectivity of local analgesia. CONCLUSIONS AND IMPLICATIONS: Targeting charged sodium channel blockers into specific sets of axons via activation of differentially expressed large-pore channels provides an opportunity to produce prolonged local analgesia, and represents an example of how exploiting ion channels as a drug delivery port can be used to increase the specificity and efficacy of therapeutics.
Assuntos
Analgésicos/farmacologia , Lidocaína/análogos & derivados , Nociceptores/efeitos dos fármacos , Células Receptoras Sensoriais/metabolismo , Bloqueadores dos Canais de Sódio/farmacologia , Canais de Cátion TRPV/antagonistas & inibidores , Analgésicos/farmacocinética , Animais , Sistemas de Liberação de Medicamentos/métodos , Lidocaína/farmacocinética , Lidocaína/farmacologia , Masculino , Nociceptores/metabolismo , Dor/tratamento farmacológico , Dor/metabolismo , Ratos , Bloqueadores dos Canais de Sódio/farmacocinética , Canais de Cátion TRPV/metabolismoRESUMO
BACKGROUND: Oesophageal acid infusion induces enhanced pain hypersensitivity in non-acid exposed upper oesophagus (secondary hyperalgesia) in patients with non-cardiac chest pain, thus suggesting central sensitisation contributes to visceral pain hypersensitivity in functional gut disorders (FGD). Perceptual wind-up (increased pain perception to constant intensity sensory stimuli at frequencies>or=0.3 Hz) is used as a proxy for central sensitisation to investigate pain syndromes where pain hypersensitivity is important (for example, fibromyalgia). AIMS: Wind-up in central sensitisation induced human visceral pain hypersensitivity has not been explored. We hypothesised that if wind-up is a proxy for central sensitisation induced human visceral pain hypersensitivity, then oesophageal wind-up should be enhanced by secondary hyperalgesia. METHODS: In eight healthy volunteers (seven males; mean age 32 years), perception at pain threshold to a train of 20 electrical stimuli applied to the hand and upper oesophagus (UO) at either 0.1 Hz (control) or 2 Hz was determined before and one hour after a 30 minute lower oesophageal acid infusion. RESULTS: Wind-up occurred only with the 2 Hz train in the UO and hand (both p=0.01). Following acid infusion, pain threshold decreased (17 (4)%; p=0.01) in the UO, suggesting the presence of secondary hyperalgesia. Wind-up to the 2 Hz train increased in the UO (wind-up ratio 1.4 (0.1) to 1.6 (0.1); p=0.03) but not in the hand (wind-up ratio 1.3 (0.1) and 1.3 (0.1); p=0.3) CONCLUSION: Enhanced wind-up after secondary oesophageal hyperalgesia suggests that visceral pain hypersensitivity induced by central sensitisation results from increased central neuronal excitability. Wind-up may offer new opportunities to investigate the contribution of central neuronal changes to symptoms in FGD.
Assuntos
Esôfago/fisiologia , Aprendizagem/fisiologia , Limiar da Dor/fisiologia , Adulto , Estimulação Elétrica , Mãos , Humanos , Ácido Clorídrico/farmacologia , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade , Medição da Dor , Distorção da PercepçãoRESUMO
Cyclooxygenase-2 (COX-2) after induction peripherally, and within the CNS, plays an important role in producing inflammatory pain. However, its role in neuropathic pain models is controversial. Recently a robust and persistent model of partial nerve injury pain, the spared nerve injury (SNI) model, has been developed. The aim of the present study was to examine the regulation of COX-2 in the rat SNI model and to evaluate the effectiveness of the selective COX-2 inhibitor rofecoxib in preventing neuropathic allodynia and hyperalgesia. RNase protection assays revealed only a very small and transient increase in COX-2 mRNA in the dorsal horn of the spinal cord in the SNI model with a maximum change at 24 h. Immunohistochemical analysis showed a small increase in COX-2 protein in the deep layers of the dorsal horn 10 h following SNI surgery. Rofecoxib (100 microM) did not affect spontaneous excitatory postsynaptic currents or alpha-amino-3-hydroxy-5-methyl-4-isoxazole propanoic acid (AMPA) and N-methyl-d-aspartate (NMDA) responses in lamina II neurons from spinal cords of animals with SNI indicating no detectable action on transmitter release or postsynaptic activity. Furthermore, rofecoxib treatment (1 and 3.2 mg/kg for 5 and 3 days respectively starting on the day of surgery) failed to modify the development of allodynia and hyperalgesia in the SNI model. However, rofecoxib significantly reduced inflammatory hypersensitivity evoked by injection of complete Freund's adjuvant into one hindpaw, indicating that the doses used were pharmacologically active. The pain hypersensitivity produced by the SNI model is not COX-2-dependent.
Assuntos
Isoenzimas/metabolismo , Neuralgia/enzimologia , Prostaglandina-Endoperóxido Sintases/metabolismo , Animais , Comportamento Animal , Ciclo-Oxigenase 2 , Inibidores de Ciclo-Oxigenase 2 , Inibidores de Ciclo-Oxigenase/análise , Inibidores de Ciclo-Oxigenase/sangue , Inibidores de Ciclo-Oxigenase/farmacologia , Soluções para Diálise/química , Modelos Animais de Doenças , Eletrofisiologia , Hiperalgesia/prevenção & controle , Hiperestesia/prevenção & controle , Imuno-Histoquímica , Isoenzimas/genética , Lactonas/análise , Lactonas/sangue , Lactonas/farmacologia , Microdiálise , Neuralgia/fisiopatologia , Ensaios de Proteção de Nucleases , Concentração Osmolar , Limiar da Dor/efeitos dos fármacos , Prostaglandina-Endoperóxido Sintases/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Ribonucleases , SulfonasRESUMO
Despite identification of GABA(B) receptors with gb1a-gb2 composition and the alpha2delta calcium channel subunit as putative molecular targets for gabapentin (GBP), its cellular mechanism of action has remained elusive. Therefore, we have used an in vitro spinal cord slice preparation to study the effects of GBP on lamina II neurons. The frequency and amplitude of spontaneous EPSCs and IPSCs were unaffected by GBP, suggesting presynaptic neurotransmitter release is not regulated. Direct modulation of postsynaptic membrane excitability is also unlikely since the level of holding current required to maintain neurons at -70, 0 and +45 mV was unaffected by GBP. Effects on excitatory and inhibitory synaptic transmission were variable across the population. Primary afferent-evoked fast glutamatergic EPSCs were unaffected by GBP, while evoked NMDA receptor-mediated EPSCs and IPSCs were variably affected. In contrast, GBP enhanced responses to bath applied NMDA in 71% of neurons. Thus, in adult rat dorsal horn, synaptic and extrasynaptic NMDA receptors may be differentially regulated by GBP perhaps due to differences in subunit composition.
Assuntos
Acetatos/farmacologia , Aminas , Ácidos Cicloexanocarboxílicos , Células do Corno Posterior/efeitos dos fármacos , Ácido gama-Aminobutírico , Animais , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Gabapentina , Técnicas In Vitro , Masculino , N-Metilaspartato/farmacologia , Células do Corno Posterior/fisiologia , Ratos , Ratos Sprague-Dawley , Transmissão Sináptica/efeitos dos fármacos , Transmissão Sináptica/fisiologiaAssuntos
Analgesia/veterinária , Analgésicos/uso terapêutico , Neurônios Aferentes/fisiologia , Dor/veterinária , Analgesia/métodos , Analgésicos/efeitos adversos , Animais , Neurônios Aferentes/classificação , Dor/fisiopatologia , Manejo da Dor , Medição da Dor/veterinária , Dor Intratável/fisiopatologia , Dor Intratável/terapia , Dor Intratável/veterináriaRESUMO
Although visceral hypersensitivity is thought to be important in generating symptoms in functional gastrointestinal disorders, the neural mechanisms involved are poorly understood. We recently showed that central sensitization (hyperexcitability of spinal cord sensory neurones) may play an important role. In this study, we demonstrate that after a 30-min infusion of 0.15 M HCl acid into the healthy human distal esophagus, we see a reduction in the pain threshold to electrical stimulation of the non-acid-exposed proximal esophagus (9.6 +/- 2.4 mA) and a concurrent reduction in the latency of the N1 and P2 components of the esophageal evoked potentials (EEP) from this region (10.4 +/- 2.3 and 15.8 +/- 5.3 ms, respectively). This reduced EEP latency indicates a central increase in afferent pathway velocity and therefore suggests that hyperexcitability within the central visceral pain pathway contributes to the hypersensitivity within the proximal, non-acid-exposed esophagus (secondary hyperalgesia/allodynia). These findings provide the first electrophysiological evidence that central sensitization contributes to human visceral hypersensitivity.
Assuntos
Sistema Nervoso Central/fisiopatologia , Esôfago/fisiopatologia , Adulto , Estimulação Elétrica , Esôfago/efeitos dos fármacos , Potenciais Evocados/efeitos dos fármacos , Feminino , Humanos , Ácido Clorídrico/farmacologia , Hiperestesia/induzido quimicamente , Hiperestesia/fisiopatologia , Masculino , Pessoa de Meia-Idade , Limiar da Dor/efeitos dos fármacos , Tempo de Reação/efeitos dos fármacos , Sensação/efeitos dos fármacos , Cloreto de Sódio/farmacologiaAssuntos
Analgésicos/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Neuropeptídeos/genética , Canais de Sódio/genética , Analgésicos/uso terapêutico , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Etiquetas de Sequências Expressas , Biblioteca Gênica , Marcação de Genes , Humanos , Canal de Sódio Disparado por Voltagem NAV1.8 , Entorpecentes/farmacologia , Neurônios Aferentes/metabolismo , Neuropeptídeos/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Dor/tratamento farmacológico , Dor/etiologia , Reação em Cadeia da Polimerase/métodos , Canais de Sódio/metabolismoRESUMO
Cutaneous sensory neurons that detect noxious stimuli project to the dorsal horn of the spinal cord, while those innervating muscle stretch receptors project to the ventral horn. DRG11, a paired homeodomain transcription factor, is expressed in both the developing dorsal horn and in sensory neurons, but not in the ventral spinal cord. Mouse embryos deficient in DRG11 display abnormalities in the spatio-temporal patterning of cutaneous sensory afferent fiber projections to the dorsal, but not the ventral spinal cord, as well as defects in dorsal horn morphogenesis. These early developmental abnormalities lead, in adults, to significantly attenuated sensitivity to noxious stimuli. In contrast, locomotion and sensori-motor functions appear normal. Drg11 is thus required for the formation of spatio-temporally appropriate projections from nociceptive sensory neurons to their central targets in the dorsal horn of the spinal cord.
Assuntos
Proteínas de Homeodomínio/metabolismo , Músculo Esquelético/inervação , Proteínas do Tecido Nervoso , Neurônios Aferentes/fisiologia , Dor/fisiopatologia , Células do Corno Posterior/fisiologia , Pele/inervação , Medula Espinal/fisiologia , Fatores de Transcrição/metabolismo , Vias Aferentes/embriologia , Vias Aferentes/fisiologia , Sequência de Aminoácidos , Animais , Animais Recém-Nascidos , Padronização Corporal , Sondas de DNA , Embrião de Mamíferos , Desenvolvimento Embrionário e Fetal , Éxons , Proteínas de Homeodomínio/genética , Temperatura Alta , Mecanorreceptores/fisiologia , Camundongos , Camundongos Knockout , Dados de Sequência Molecular , Atividade Motora , Mutagênese Sítio-Dirigida , Nociceptores/fisiologia , Ratos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Transcrição/deficiência , Fatores de Transcrição/genéticaRESUMO
The development of neuronal excitability involves the coordinated expression of different voltage-gated ion channels. We have characterized the expression of two sensory neuron-specific tetrodotoxin-resistant sodium channel alpha subunits, Na(v)1. (SNS/PN3) and Na(v)1.9 (SNS2/NaN), in developing rat lumbar dorsal root ganglia (DRGs). Expression of both Na(v)1.8 and Na(v)1.9 increases with age, beginning at embryonic day (E) 15 and E17, respectively, and reaching adult levels by postnatal day 7. Their distribution is restricted mainly to those subpopulations of primary sensory neurons in developing and adult DRGs that give rise to unmyelinated C-fibers (neurofilament 200 negative). Na(v)1.8 is expressed in a higher proportion of neuronal profiles than Na(v)1.9 at all stages during development, as in the adult. At E17, almost all Na(v)1.8-expressing neurons also express the high-affinity NGF receptor TrkA, and only a small proportion bind to IB4, a marker for c-ret-expressing (glial-derived neurotrophic factor-responsive) neurons. Because IB4 binding neurons differentiate from TrkA neurons in the postnatal period, the proportion of Na(v)1.8 cells that bind to IB4 increases, in parallel with a decrease in the proportion of Na(v)1.8-TrkA co-expressing cells. In contrast, an equal number of Na(v)1.9 cells bind IB4 and TrkA in embryonic life. The differential expression of Na(v)1.8 and Na(v)1.9 in late embryonic development, with their distinctive kinetic properties, may contribute to the development of spontaneous and stimulus-evoked excitability in small diameter primary sensory neurons in the perinatal period and the activity-dependent changes in differentiation they produce.
Assuntos
Gânglios Espinais/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Neurônios Aferentes/metabolismo , Neuropeptídeos/metabolismo , Canais de Sódio/metabolismo , Envelhecimento/metabolismo , Animais , Antígenos de Diferenciação/análise , Antígenos de Diferenciação/biossíntese , Northern Blotting , Gânglios Espinais/citologia , Gânglios Espinais/embriologia , Imuno-Histoquímica , Canal de Sódio Disparado por Voltagem NAV1.8 , Canal de Sódio Disparado por Voltagem NAV1.9 , Neurônios Aferentes/classificação , Neurônios Aferentes/citologia , Neurônios Aferentes/efeitos dos fármacos , Neuropeptídeos/efeitos dos fármacos , Neuropeptídeos/genética , Subunidades Proteicas , RNA Mensageiro/análise , RNA Mensageiro/biossíntese , Ratos , Ratos Sprague-Dawley , Receptor trkA/análise , Receptor trkA/biossíntese , Canais de Sódio/efeitos dos fármacos , Canais de Sódio/genética , Tetrodotoxina/farmacologiaRESUMO
Excitotoxicity due to excessive synaptic glutamate release is featured in many neurological conditions in which neuronal death occurs. Whether activation of primary sensory pathways can ever produce sufficient over-activity in secondary sensory neurons in the dorsal horn of the spinal cord to induce cell death, however, has not been determined. In this study, we asked whether activity in myelinated afferents (A fibers), which use glutamate as a transmitter, can induce cell death in the dorsal horn. Using stereological estimates of neuron numbers from electron microscopic sections, we found that stimulation of A-fibers in an intact sciatic nerve at 10 Hz, 20 Hz, and 50 Hz in 10-minute intervals at a stimulus strength that activates both Abeta and Adelta fibers resulted in the loss of 25% of neurons in lamina III, the major site of termination of large Abeta fibers, but not in lamina I, where Adelta fibers terminate. Furthermore, sciatic nerve lesions did not result in detectable neuron loss, but activation of A fibers in a previously sectioned sciatic nerve did cause substantial cell death not only in lamina III but also in laminae I and II. The expansion of the territory of A-fiber afferent-evoked cell death is likely to reflect the sprouting of the fibers into these laminae after peripheral nerve injury. The data show, therefore, that primary afferent A-fiber activity can cause neuronal cell death in the dorsal horn with an anatomical distribution that depends on whether intact or injured fibers are activated. Stimulation-induced cell death potentially may contribute to the development of persistent pain.
Assuntos
Vias Aferentes/metabolismo , Morte Celular/fisiologia , Ácido Glutâmico/metabolismo , Fibras Nervosas Mielinizadas/metabolismo , Células do Corno Posterior/metabolismo , Transmissão Sináptica/fisiologia , Vias Aferentes/ultraestrutura , Animais , Contagem de Células , Estimulação Elétrica/efeitos adversos , Masculino , Microscopia Eletrônica , Fibras Nervosas Mielinizadas/ultraestrutura , Plasticidade Neuronal/fisiologia , Dor/etiologia , Dor/patologia , Dor/fisiopatologia , Doenças do Sistema Nervoso Periférico/patologia , Doenças do Sistema Nervoso Periférico/fisiopatologia , Células do Corno Posterior/ultraestrutura , Ratos , Ratos Sprague-Dawley , Nervo Isquiático/patologia , Nervo Isquiático/fisiopatologia , Nervo Isquiático/cirurgiaRESUMO
Mammalian brain sodium channel (BNaC, also known as BNC/ASIC) proteins form acid-sensitive and amiloride-blockable sodium channels that are related to putative mechanosensory channels. Certain BNaC isoforms are expressed exclusively in dorsal root ganglia (DRG) and have been proposed to form the ion channels mediating tissue acidosis-induced pain. With antibody labeling, we find that the BNaC1alpha isoform is expressed by most large DRG neurons (low-threshold mechanosensors not involved in acid-induced nociception) and few small nociceptor neurons (which include high-threshold mechanoreceptors). BNaC1alpha is transported from DRG cell bodies to sensory terminals in the periphery, but not to the spinal cord, and is located specifically at specialized cutaneous mechanosensory terminals, including Meissner, Merkel, penicillate, reticular, lanceolate, and hair follicle palisades as well as some intraepidermal and free myelinated nerve endings. Accordingly, BNaC1alpha channels might participate in the transduction of touch and painful mechanical stimuli.
Assuntos
Gânglios Espinais/metabolismo , Canais Iônicos/metabolismo , Mecanorreceptores/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Neurônios Aferentes/metabolismo , Canais de Sódio/metabolismo , Canais Iônicos Sensíveis a Ácido , Animais , Células CHO , Clonagem Molecular , Cricetinae , Canais de Sódio Degenerina , Canais Epiteliais de Sódio , Gânglios Espinais/citologia , Canais Iônicos/genética , Masculino , Mecanorreceptores/citologia , Camundongos , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/genética , Neurônios Aferentes/citologia , Especificidade de Órgãos , Técnicas de Patch-Clamp , Mapeamento Físico do Cromossomo , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Transporte Proteico , Ratos , Ratos Sprague-Dawley , Pele/citologia , Pele/inervação , Pele/metabolismo , Canais de Sódio/genética , TransfecçãoRESUMO
Inflammation causes the induction of cyclooxygenase-2 (Cox-2), leading to the release of prostanoids, which sensitize peripheral nociceptor terminals and produce localized pain hypersensitivity. Peripheral inflammation also generates pain hypersensitivity in neighbouring uninjured tissue (secondary hyperalgesia), because of increased neuronal excitability in the spinal cord (central sensitization), and a syndrome comprising diffuse muscle and joint pain, fever, lethargy and anorexia. Here we show that Cox-2 may be involved in these central nervous system (CNS) responses, by finding a widespread induction of Cox-2 expression in spinal cord neurons and in other regions of the CNS, elevating prostaglandin E2 (PGE2) levels in the cerebrospinal fluid. The major inducer of central Cox-2 upregulation is interleukin-1beta in the CNS, and as basal phospholipase A2 activity in the CNS does not change with peripheral inflammation, Cox-2 levels must regulate central prostanoid production. Intraspinal administration of an interleukin-converting enzyme or Cox-2 inhibitor decreases inflammation-induced central PGE2 levels and mechanical hyperalgesia. Thus, preventing central prostanoid production by inhibiting the interleukin-1beta-mediated induction of Cox-2 in neurons or by inhibiting central Cox-2 activity reduces centrally generated inflammatory pain hypersensitivity.
Assuntos
Sistema Nervoso Central/fisiologia , Inflamação/fisiopatologia , Interleucina-1/fisiologia , Isoenzimas/metabolismo , Dor , Prostaglandina-Endoperóxido Sintases/metabolismo , Sistema Nervoso Central/enzimologia , Ciclo-Oxigenase 2 , Dinoprostona/líquido cefalorraquidiano , Indução Enzimática , Adjuvante de Freund , Inflamação/metabolismo , Neurônios/enzimologia , Neurônios/fisiologia , Medula Espinal/citologia , Medula Espinal/enzimologia , Medula Espinal/fisiologiaRESUMO
Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.
Assuntos
Proteínas de Transporte de Cátions , Canais Iônicos , Receptores de Droga/genética , Sequência de Aminoácidos , Animais , Northern Blotting , Linhagem Celular , Mapeamento Cromossômico , Cromossomos Humanos Par 12/genética , Clonagem Molecular , DNA Complementar/química , DNA Complementar/genética , DNA Complementar/isolamento & purificação , Feminino , Humanos , Imuno-Histoquímica , Hibridização in Situ Fluorescente , Masculino , Dados de Sequência Molecular , RNA/genética , RNA/metabolismo , Mapeamento de Híbridos Radioativos , Ratos , Receptores de Droga/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Canais de Cátion TRPV , Distribuição TecidualRESUMO
Pathological pain, consisting of tissue injury-induced inflammatory and nerve injury-induced neuropathic pain, is an expression of neuronal plasticity. One component of this is that the afferent input generated by injury and intense noxious stimuli triggers an increased excitability of nociceptive neurons in the spinal cord. This central sensitization is an activity-dependent functional plasticity that results from activation of different intracellular kinase cascades leading to the phosphorylation of key membrane receptors and channels, increasing synaptic efficacy. Central sensitization is both induced and maintained in a transcription-independent manner. Several different intracellular signal transduction cascades converge on MAPK (mitogen-activated protein kinase), activation of which appears to be a master switch or gate for the regulation of central sensitization. In addition to posttranslational regulation, the MAPK pathway may also regulate long-term pain hypersensitivity, via transcriptional regulation of key gene products. Pharmacological intervention targeted specifically at the signal transduction pathways in nociceptive neurons may provide, therefore, new therapeutic opportunities for pathological pain.