Nano- and micro-plastic transport in soil and groundwater environments: Sources, behaviors, theories, and models.

With the increasing use of plastics, nano- and micro-plastic (NMP) pollution has become a hot topic in the scientific community. Ubiquitous NMPs, as emerging contaminants, are becoming a global issue owing to their persistence and potential toxicity. Compared with studies of marine and freshwater environments, investigations into the sources, transport properties, and fate of NMPs in soil and groundwater environments remain at a primary stage. Hence, the promotion of such research is critically important. Here, we integrate existing information and recent advancements to compile a comprehensive evaluation of the sources and transport properties of NMPs in soil and groundwater environments. We first provide a systematic description of the various sources and transport behaviors of NMPs. We then discuss the theories (e.g., clean-bed filtration and Derjaguin-Landau-Verwey-Overbeek theories) and models (e.g., single-site and dual-site kinetic retention and transport models) of NMP transport through saturated porous media. Finally, we outline the potential limitations of current research and suggest directions for future research. Overall, this review intends to assimilate and outline current knowledge and provide a useful reference frame to determine the sources and transport properties of NMPs in soil and groundwater environments.

Synergistic degradation for o-chlorophenol and enhancement of power generation by a coupled photocatalytic-microbial fuel cell system.

A hierarchically photocatalytic microbial fuel cell system (PMFC) coupled with TiO2 photoanode and bioanode was established to enhance the power generation based on single-chamber MFC. Compared with the conventional anaerobic mode, oxygen in the solution could be utilized by the photoanode of PMFC to improve the removal of o-chlorophenol (2-CP). The maximum power densities were increasing from 261 (MFC) to 301 mW/m2 (PMFC). The removal efficiency of 2-CP (5 mg/L) in PMFC was 76.20% and higher than that in MFC (19.33%) and by photocatalysis (49.23%). The electron-hole separation efficiencies were decreasing with the increasing of dissolved oxygen, causing a low efficiency of photocatalysis, due to the reduction of the current density of the systems. The abundance of Geobacter sp., PHOS-HE36 fam., and Pseudomonas sp. was increased with illumination, contributing to improve the electricity production and 2-CP degradation. The only detective intermediate of 1,2-dichlorobenzene in PMFC indicated that the microbes could regulate the degradation pathway of 2-CP in the coupling system. These findings provided an feasible method for the effective degradation of refractory organic compounds and simultaneous energy recovery by combining photocatalysis and microbial power generation.

A sensitive electrochemical sensor for environmental toxicity monitoring based on tungsten disulfide nanosheets/hydroxylated carbon nanotubes nanocomposite.

There has been growing concern about the toxic effects of pollutants in the aquatic environment. In this study, a novel cell-based electrochemical sensor was developed to detect the toxicity of contaminants in water samples. A screen-printed carbon electrode, which was low-cost, energy-efficient, and disposable, was modified with tungsten disulfide nanosheets/hydroxylated multi-walled carbon nanotubes (WS2/MWCNTs-OH) to improve electrocatalytic performance and sensitivity. The surface morphology, structure, and electrochemical property of WS2/MWCNTs-OH composite film were characterized by emission scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, X-ray diffraction, Raman spectroscopy, and electrochemical impedance spectroscopy. Grass carp kidney cell line was utilized as the sensor biorecognition element to determine the electrochemical signals and evaluate cell viability. The sensor was used to detect the toxicity of one typical contaminant (2,4,6-trichlorophenol) and two emerging contaminants (bisphenol AF and polystyrene nanoplastics). The 48 h half inhibitory concentration (IC50) values were 169.96 µM, 21.88 µM, and 123.01 µg mL-1, respectively, which were lower than those of conventional MTT assay, indicating the higher sensitivity of the proposed sensor. Furthermore, the practical application of the sensor was evaluated in chemical wastewater samples. This study provides an up-and-coming tool for environmental toxicity monitoring.

Cytotoxicity and action mechanisms of polycyclic aromatic hydrocarbons by a miniature electrochemical detection system.

The effects of six polycyclic aromatic hydrocarbons (PAHs) on the activity of V79 cells were studied by using a miniature electrochemical system based on graphene oxide quantum dots and multiwall carbon nanotubes modified anodized screen printed carbon electrode. The cytotoxicity sequence of PAHs on V79 cells was different with guanine/xanthine (G/X), adenine (A), hypoxanthine (HX), and the end product of purine nucleotide catabolism, uric acid (UA), as biomarkers. The IC50 values measured with UA as the biomarker were the lowest, indicating that UA in cells was more sensitive to PAHs. The cytotoxicity sequence with G/X as the biomarker was the same as that of the MTT assay: pyrene > phenanthrene > benzo[a]pyrene > fluoranthene > fluorene > naphthalene. The cytotoxicity sequences measured by different biomarkers varied, which related to different structures that may influence the expression of the cellular aryl hydrocarbon receptor, gap junctional intercellular communication, and p53 protein. PAHs with different structures played varied roles in cell development and differentiation. Additionally, the electrochemical method was more sensitive than the MTT assay. The miniature electrochemical system enabled the simultaneous detection of four signals in cells, providing more information for multi-parameter evaluation and toxic mechanism study of PAHs and other pollutants.

Cytotoxicity assessment of antibiotics on Ctenopharyngodon idellus kidney cells by a sensitive electrochemical method.

As emerging pollutants, antibiotics are ubiquitous in the environment and pose a threat to human health, giving rise to an urgent need to assess their biological toxicity. In the present study, a cell electrochemical method based on the bromocresol violet/carbon nanotubes/glassy carbon electrode (BCP/MWCNTs/GCE) was established to evaluate the cytotoxicities of sulfamethoxazole (SMZ), ciprofloxacin (CIP), and tetracycline (TC). BCP/MWCNTs/GCE has advantages due to its excellent electrocatalytic activity for the oxidation of electroactive species of the Ctenopharyngodon idellus kidney (CIK) cells. The half-maximal inhibitory concentration (IC50) values of SMZ, CIP, and TC obtained by the electrochemical method were 831.51 µM, 354.98 µM, and 184.51 µM, which were lower than those of the traditional methyl-thiazolyl-tetrazolium (MTT) assay (907.47 µM, 414.87 µM, and 208.11 µM). These results indicate the higher sensitivity of the electrochemical method. This study provided a sensitive tool for the cytotoxicity evaluation of antibiotics in the environmental toxicology field.

Evaluation of single and combined toxicity of bisphenol A and its analogues using a highly-sensitive micro-biosensor.

Bisphenol analogues have been developed as alternatives to bisphenol A (BPA), a common chemical with potential adverse effects on human health. It is imperative to perform a fast and sensitive evaluation for the toxicity of these bisphenol analogues. This study introduces a label-free electrochemical biosensor based on a screen-printed electrode modified with the carboxylated multi-walled carbon nanotube/rhodamine B/gold nanoparticle. Ctenopharyngodon idella kidney (CIK) cells were used as the biological recognition agent to detect changes in electrochemical signals and indicate the cell viability. Only 20 µL of sample was required for detection, which was much lower than that of other conventional electrochemical methods (≥ 1 mL). This biosensor was examined for the cytotoxicity of BPA, bisphenol AF (BPAF), bisphenol B (BPB), bisphenol F (BPF), and bisphenol S (BPS) to CIK cells. The half inhibition concentration (IC50) values after 48 h of exposure indicated that the rank order of cytotoxicities was BPAF > BPB > BPA > BPF > BPS. The morphological changes in CIK cells after treatment with various bisphenols were investigated, and the combined toxicities of the binary bisphenol mixtures were determined. Potentially synergistic and additive effects were observed. These findings provide new insights into the cytotoxicity of bisphenol analogues.

Pollution characteristics of antibiotics and antibiotic resistance of coliform bacteria in the Yitong River, China.

In this study, the concentrations of nine typical antibiotics, including sulfadiazine (SD), sulfamerazine (SMR), sulfamethazine (SM2), sulfamethoxazole (SMZ), ofloxacin (OFX), ciprofloxacin (CIP), trimethoprim (TMP), oxytetracycline (OTC), and tetracycline hydrochloride (TC), were detected in the Yitong River by solid-phase extraction high-performance liquid chromatography. The concentrations of the antibiotics were analyzed. Additionally, an improved immobilized substrate enzyme substrate method (DST-enzyme substrate method) was developed and used to evaluate the antibiotic resistance of coliform bacteria to OFX, CIP, enrofloxacin (ENR), TC, sulfisoxazole (SOX), and TMP in the Yitong River. The results showed that the concentrations of the nine antibiotics ranged from nd (not detected) to 1.361 µg/L. The detection rate and concentration of OFX were the highest, followed by CIP, and the detection rate and concentration of SM2 and OTC were the lowest. The detection rate and concentrations of antibiotics were higher in August and November than those in May. The antibiotics were mainly distributed in the livestock sewage discharge and suburban domestic sewage discharge areas. Moreover, the drug resistance of total coliform bacteria to fluoroquinolones, sulfonamides, tetracyclines, and TMP varied with season.

A miniaturized electrochemical toxicity biosensor based on graphene oxide quantum dots/carboxylated carbon nanotubes for assessment of priority pollutants.

The study presented a sensitive and miniaturized cell-based electrochemical biosensor to assess the toxicity of priority pollutants in the aquatic environment. Human hepatoma (HepG2) cells were used as the biological recognition agent to measure the changes of electrochemical signals and reflect the cell viability. The graphene oxide quantum dots/carboxylated carbon nanotubes hybrid was developed in a facile and green way. Based on the hybrid composite modified pencil graphite electrode, the cell culture and detection vessel was miniaturized to a 96-well plate instead of the traditional culture dish. In addition, three sensitive electrochemical signals attributed to guanine/xanthine, adenine, and hypoxanthine were detected simultaneously. The biosensor was used to evaluate the toxicity of six priority pollutants, including Cd, Hg, Pb, 2,4-dinitrophenol, 2,4,6-trichlorophenol, and pentachlorophenol. The 24h IC50 values obtained by the electrochemical biosensor were lower than those of conventional MTT assay, suggesting the enhanced sensitivity of the electrochemical assay towards heavy metals and phenols. This platform enables the label-free and sensitive detection of cell physiological status with multi-parameters and constitutes a promising approach for toxicity detection of pollutants. It makes possible for automatical and high-throughput analysis on nucleotide catabolism, which may be critical for life science and toxicology.