RESUMO
OBJECTIVE: To study the pathogenesis of viral myocarditis (VMC) and dilated cardiomyopathy (DCM) and their relationship. METHODS: Sixty samples including 20 VMC, 20 DCM and 20 controls were collected. The expression of Fas protein in myocardium of each group was detected by modified immunohistochemistry with unequivocal brown staining in the myocardial membrane scored as positive, and the results of positive reaction were analyzed by Ridit test. RESULTS: Fas protein expression increased obviously in VMC and DCM groups as compared with that of the control group. The difference of positive results between each group analyzed by Ridit test was statistically significant (P<0.005). Statistically significant differences were found between VMC and control groups as well as between DCM and control groups (P<0.05), but not between VMC and DCM groups (P>0.05) by multiple comparison Ridit test. CONCLUSION: The expression of Fas protein is significantly higher in the VMC and DCM groups than in that of the control group. These results suggest that both the VMC and DCM may share a similar pathogenesis, which most likely involves cell apoptosis.
Assuntos
Apoptose/fisiologia , Cardiomiopatia Dilatada/metabolismo , Miocardite/metabolismo , Miocardite/virologia , Receptor fas/metabolismo , Cardiomiopatia Dilatada/patologia , Estudos de Casos e Controles , Feminino , Patologia Legal , Humanos , Masculino , Miocardite/patologia , Receptor fas/genéticaRESUMO
OBJECTIVE: To evaluate the expression of Bcl-2 protein after brain concussion. METHODS: Expression levels of Bel-2 protein in cortex, pontine and cerebellum of rats were investigated using immunohistochemistry. RESULTS: There was no expression of Bcl-2 protein in control group seen. The expression of Bcl-2 protein in brain concussion groups was detected at l hour, and the expression level reached its peak 4 days after the concussion and then declined gradually. CONCLUSION: Our findings suggest that the detection of Bel-2 protein could be an indicator for diagnosis of brain concussion and for estimation of the post injury time interval.
Assuntos
Concussão Encefálica/metabolismo , Tronco Encefálico/metabolismo , Córtex Cerebral/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Tronco Encefálico/patologia , Córtex Cerebral/patologia , Contusões/metabolismo , Contusões/patologia , Modelos Animais de Doenças , Feminino , Imuno-Histoquímica , Masculino , Neurônios/metabolismo , Neurônios/patologia , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
OBJECTIVE: To observe pathological changes and apoptosis in rats myocardial cells after Macleaya cordata total alkaloids poisoning, and to provide some references for Macleaya cordata total alkaloids poisoning detection. METHODS: An experimental model of Macleaya cordata total alkaloids poisoning was established, and the technology of TUNEL staining was used.The results were analyzed by computer image analysis competitive system. RESULTS: Quantities of apoptosis in myocardial cells in poisoning groups were much more than those in the control groups at different tages (P<0.01). In addition the quantities of apoptosis were different after different poisoning duration. CONCLUSION: Although clinical symptoms was not obvious and could not be detected by poison analysis. Pathological changes induced by Macleaya cordata total alkaloids could be found through the apoptosis detection.
