Biomarkers for the prediction of major adverse cardiovascular events in patients with acute coronary syndrome.

The aim of this study is to find novel biomarkers for prognosis in patients with acute coronary syndrome (ACS). We enrolled 245 eligible patients with ACS and compared the protein expression between the two groups, with or without major adverse cardiovascular events (MACE). To determine biomarkers for prognosis, we performed mass spectrometry analysis. We found 10 proteins in the serum that can be used to classify ACS with or without MACE and specific protein peaks at m/z 1921.0, 2124.2, and 20887.3 that were increased in patients with MACE. These peaks reliably predict MACE occurrence in patients with ACS, in addition to the widely accepted markers troponin and brain natriuretic peptide precursor. The characteristic changes in the peaks at m/z 1921.09, 2124.2, and 20887.3 correlate with poor prognosis in ACS patients. These proteins could potentially be used as predictive biomarkers to evaluate patient prognosis.

[FTIR spectroscopic explorations of freshly resected laryngeal carcinoma tissues].

OBJECTIVE: To investigate the characteristics of freshly resected laryngeal carcinoma by Fourier transform infrared spectroscopy (FTIR). METHODS: FTIR was applied to the study of the cancerous tissues and adjacent normal tissues in 32 patients. RESULTS: Compared with pathological diagnosis results, one benign specimen was classified as a malignant, the accuracy was 98.4%. Significant differences were seen in the FTIR spectra between the normal and malignant laryngeal tissues. The peak at 1085 cm(-1) shift to 1114 cm(-1) showed that the relative contents of DNA in laryngeal carcinoma cells was increased. The peak at 1397 cm(-1) was stronger than 1451 cm(-1) in normal tissues, while it was not obvious in cancer tissues. I(2926)/I(2870) in carcinoma cells was lower than that in normal tissues. The wave numbers of the bands of amide I and amide II, symmetric and asymmetric stretching bands of CH(3), stretching vibration bands of C-OH and NH band were shifted to higher number in cancer tissues. CONCLUSION: The study shows that the malignant and normal laryngeal tissues have different FTIR spectra, which are mainly due to changes in protein, nucleic acid and phospholipids. FTIR may become a new method for the diagnosis of laryngeal carcinoma in clinical practice.

[Clinical study on the therapeutic effects and mechanism of progesterone in the treatment for acute severe head injury].

OBJECTIVE: To evaluate the therapeutic effects of Progesterone (PG) on the patients with acute severe traumatic brain injury, and investigate it's neuroprotective mechanisms. METHODS: Fifth-six patients with acute severe traumatic head injury were divided randomly into two groups: 26 cases were treated with PG and 30 cases were control. Neurological outcome of the patients were assessed using Glasgow Coma Scale (GCS), Glasgow Outcome Scale (GOS), verbal and motor functions scale and Karnofsky Performance Scale (KPS). The serum concentrations of PG, TNF-alpha and 15-F(2t)-isoprostane were measured at day 1, 5 and 10 after trauma. RESULTS: In the two groups, There were no significant difference in the mortality, GCS of acute healing phase, GOS and verbal and motor functions at 10th days after treatment (P>0.05); After follow-up for 3 months, GOS, verbal functions and KPSin the PG treatment group were better than those in the control group (P<0.05); In addition, there was no difference of motor functions in the two groups (P>0.05). At 5th day after trauma, serum 15-F(2t)-isoprostane and TNF-alpha levels increased in the control group, but decreased at 10th day after trauma. Compared with the control group serum PG levels increased, serum 15-F(2t)-isoprostane and TNF-alpha levels reduced significantly in the PG treatment group at 5th and 10th day after injury (P<0.05). CONCLUSION: It indicated that successive early application of PG will benefit the patients with acute severe head injury by improving the recovery and reducing the disability, which may be related to its alleviating inflammatory and lipid peroxidation response.

[Expression of survivin gene and its relationship with expression of p15, p16 proteins in laryngeal squamous cell carcinomas].

OBJECTIVE: : To investigate the expression of Survivin (SVV) and its relationship with expression of p15, p16 in laryngeal squamous cell carcinoma (LSCC). METHODS: Using strep avidin-biotin complex (SABC) method, we examined the expression of SVV, p15 and p16 gene in 48 LSCC tissus samples, 24 normal laryngeal mucosa tissus samples and 24 normal laryngeal tissus adjacent to the tumors samples. RESULTS: SVV was expression in 27 of 48 (56.3%) samples of LSCC and expression in 6 of 24 (25.0%) samples of normal laryngeal tissus adjacent to the tumors. Normal laryngeal mocosa tissus samples did not expressed SVV. Overexpression of SVV was related to the tumor site, grade, clinical stage and tumor prognosis (P < 0.05). The expression of SVV in LSCC was positive correlated with p16 expression( C = 0.52 P < 0.001), but not with p15 expression. CONCLUSION: SVV may play a role in the pathway of carcinogenesis and tumor progress. It is feasible for early diagnosis and prognosis estimation. Overexpression of SVV gene and de-activation of antioncogene p16 may be play synergetic roles in the carcinogenesis of LSCC.

[Study on the expression of the metastasis suppressor gene KAI1 in laryngeal squamous cell carcinoma].

OBJECTIVE: To investigate the expression of the metastasis suppressor gene KAI1 in laryngeal squamous cell carcinoma and the relationship between expression of KAI1 and it's role in tumorgenesis and development. METHODS: The sections of 84 samples of primary laryngeal squamous cell carcinoma (PLSCC), 27 samples of laryngeal precancerous lesion (LPL), 10 samples of vocal cords polyp (VCP) and 10 samples of normal laryngeal tissues (NLT) were examined by in situ hybridization for KAI1mRNA. RESULTS: It was found that 10 samples of NLT and 10 samples of VCP showed strong expression of KAI1, the Integrated A Total of KIA1 in these two types of tissues were 136,206.8 +/- 36,675.536 and 133,674.505 +/- 42,858.456, respectively, with no statistically significant difference between them, and high prevalence of decreased expression of KAI1 in LPL and PLSCC compared with that in VCP and in NLT (P < 0.01). There was a significant relationship between down-regulation of KAI1 expression with pathological grade, lymph node metastases, T stage and clinical stage (P < 0.01) and did not correlate with gender. CONCLUSION: KAI1 may play an important role on tumor genesis, development, invasion and metastasis of PLSCC, and become one of the molecular markers for early diagnosis and predicting of aggressive and metastasis potential to assess the clinical stage of PLSCC.