Investigating the illicit market in veterinary medicines: An exploratory online study with pet owners in the United Kingdom.

The illicit market in veterinary medicines is an overlooked issue despite threatening the health of non-human and human animals. It is thought to be increasing within the major markets of the global North due to the growth of e-commerce and social media sites. This paper examines the online market in illicit veterinary medicines through an exploratory study of the public's online experiences as pet owners in the UK. To this end, we collected data through literature-based research and an online survey. Drawing on Passas' criminogenic asymmetries framework, the research found that the confluence of legal, political, cultural, economic and knowledge asymmetries likely facilitate the market in illicit veterinary medicines in the UK. Our research concludes that, while previous reports suggest the illicit market is dominated by medicines to treat pets, it increasingly consists of medicines for farmed animals. This brings its own set of challenges and risks, and a pressing need for further research on the market's dynamics.

The welfare of wildlife: an interdisciplinary analysis of harm in the legal and illegal wildlife trades and possible ways forward.

Wildlife trade-both legal and illegal-is an activity that is currently the focus of global attention. Concerns over the loss of biodiversity, partly stemming from overexploitation, and the corona virus pandemic, likely originating from wildlife trade, are urgent matters. These concerns though centre on people. Only sometimes does the discussion focus on the wildlife traded and their welfare. In this article, we make the case as to why welfare is an important component of any discussion or policy about wildlife trade, not only for the interests of the wildlife, but also for the sake of humans. We detail the harm in the trade as well as the current welfare provisions, particularly in relation to the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), which guide global transport and trade. There are a number of ways that the current approach to wildlife welfare could be improved, and we propose ways forward in this regard.

European illegal puppy trade and organised crime.

Organised crime groups' involvement in illicit markets is a common focus of law enforcement and governments. Drug, weapon, human and wildlife trafficking (and others) are all illegal activities with link to organised crime. This paper explores the overlooked illicit market of puppies. We detail the state of knowledge about the organisation of the UK puppy trade, which includes irresponsible and illegal breeding of puppies throughout Europe and their often-illegal movement into the UK. In 2017, we conducted an analysis of hundreds of online advertisements in Scotland, 12 expert interviews, a stakeholder survey of 53 participants, and 40 focus groups across Great Britain. Our data suggest an organised illicit market running in parallel to the legal trade. We speculate as to whether at some point along the supply chain organised crime groups are responsible for the suffering and death of the puppies and the economic and emotional damage to 'consumers'. Online monitoring and physical scrutiny at the ports must be improved to reduce non-human animal abuse. People buying puppies must also be made aware that their purchase could be profiting organised crime.

Are Fish Wild?

As the global biodiversity crisis continues, it is important to examine the legislative protection that is in place for species around the world. Such legislation not only includes environmental or wildlife law, but also trade law, such as the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), which gets transposed into national legislation. This commentary analyses legislative definitions of wildlife, whether or not that includes fish, which has implications for fish welfare, use of fish for food security, and biodiversity conservation when fish, or other wildlife, are excluded. Through a legislative content analysis of the 183 parties' legislation of CITES, we explore whether fish are afforded the same protections as other species by being included in legal definitions of wildlife. We found that while a majority of CITES parties' legislation appear to define fish as wildlife, there are a number of instances where this is unclear or not the case, and this could have significant ramifications for the welfare of non-human animals, their use, and conservation.

Connections between trades and trafficking in wildlife and drugs.

Whilst drug trafficking has been a concern for several decades, wildlife trafficking has only fairly recently garnered international attention. Often media coverage of wildlife trafficking links it to the illegal trade of drugs. This article analyses wildlife and drug trafficking connections of various kinds. The purpose is to reveal the overlaps and synergies of wildlife and drug trafficking, providing concrete examples of where these markets co-exist as well as intertwine based on literature and original fieldwork. It explores the question of 'Why in some cases, an illicit market remains focused on a single commodity, whilst in others it accommodates a combination of illicit commodities?' This study identifies different types of wildlife-drugs linkages, including combined contraband, camouflage, multiple trade lines, shared smuggling routes and transportation methods, barter trade, and laundering drug money. The article shows that illicit markets are complex and the examples of activities and transactions that are provided illuminate some of the different dimensions of converging and diverging trades involving wildlife and drugs.

Canada and the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES): Lessons Learned on Implementation and Compliance.

Unsustainable and illegal wildlife trade are contributing to the unprecedented levels of biodiversity loss and possible extinction of one million species. Law enforcement and the criminal justice system have a role to play in helping to regulate and monitor such trade. The main international instrument to regulate wildlife trade is the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). This mixed methods study researched the lessons learned and best practice in regards to implementation of and compliance with CITES. As part of the study, three countries were identified as case studies and Canada was selected as one of these. Lessons can also be learned from Canada's Wild Animal and Plant Protection and Regulation of International and Interprovincial Trade Act, which is cumbersome to update when species protections change within CITES. Canada has several elements of good practice, such as the remit, effectiveness and relationships of the three CITES authorities located within Environment and Climate Change Canada, the public health approach to some wildlife imports, and the protection of native CITES species. CITES needs to be improved to further protect endangered species and lessons from Canada and other countries can contribute to this improvement.

Human motor neuron progenitor transplantation leads to endogenous neuronal sparing in 3 models of motor neuron loss.

Motor neuron loss is characteristic of many neurodegenerative disorders and results in rapid loss of muscle control, paralysis, and eventual death in severe cases. In order to investigate the neurotrophic effects of a motor neuron lineage graft, we transplanted human embryonic stem cell-derived motor neuron progenitors (hMNPs) and examined their histopathological effect in three animal models of motor neuron loss. Specifically, we transplanted hMNPs into rodent models of SMA (Δ7SMN), ALS (SOD1 G93A), and spinal cord injury (SCI). The transplanted cells survived and differentiated in all models. In addition, we have also found that hMNPs secrete physiologically active growth factors in vivo, including NGF and NT-3, which significantly enhanced the number of spared endogenous neurons in all three animal models. The ability to maintain dying motor neurons by delivering motor neuron-specific neurotrophic support represents a powerful treatment strategy for diseases characterized by motor neuron loss.

Stem cell-derived neurotrophic support for the neuromuscular junction in spinal muscular atrophy.

IMPORTANCE OF THE FIELD: Spinal muscular atrophy (SMA) is a neurodegenerative disease characterized by specific degeneration of α-motor neurons in the spinal cord. The use of cell transplantation to restore lost function through cell replacement or prevent further degeneration of motor neurons and synapses through neurotrophic support heralds tremendous hope in the SMA field. AREAS COVERED IN THIS REVIEW: Much research has been carried out in the last decade on the use of embryonic stem cells in cell replacement strategies for various neurodegenerative diseases. Cell replacement is contingent on the ability of transplanted cells to integrate and form new functional connections with host cells. In the case of SMA, cell replacement is a tall order in that axons of transplanted cells would be required to grow over long distances from the spinal cord through growth-averse terrain to synapse with muscles in the periphery. The efficacy of neurotrophic support is contingent on the ability of transplanted cells to secrete neurotrophins appropriate for degenerating motor neurons in the spinal cord or development/stability of the neuromuscular junction (NMJ) in the periphery. WHAT THE READER WILL GAIN: The reader will gain an understanding of the potential of neurotrophins to promote development of the NMJ in a diseased or injured environment. TAKE HOME MESSAGE: Neurotrophins play a major role in NMJ development and thus may be a key factor in the pathogenesis of NMJs in SMA. Further research into the signaling mechanisms involved in NMJ maturation may identify additional mechanisms by which transplanted cells may be of therapeutic benefit.

Histological and functional benefit following transplantation of motor neuron progenitors to the injured rat spinal cord.

BACKGROUND: Motor neuron loss is characteristic of cervical spinal cord injury (SCI) and contributes to functional deficit. METHODOLOGY/PRINCIPAL FINDINGS: In order to investigate the amenability of the injured adult spinal cord to motor neuron differentiation, we transplanted spinal cord injured animals with a high purity population of human motor neuron progenitors (hMNP) derived from human embryonic stem cells (hESCs). In vitro, hMNPs displayed characteristic motor neuron-specific markers, a typical electrophysiological profile, functionally innervated human or rodent muscle, and secreted physiologically active growth factors that caused neurite branching and neuronal survival. hMNP transplantation into cervical SCI sites in adult rats resulted in suppression of intracellular signaling pathways associated with SCI pathogenesis, which correlated with greater endogenous neuronal survival and neurite branching. These neurotrophic effects were accompanied by significantly enhanced performance on all parameters of the balance beam task, as compared to controls. Interestingly, hMNP transplantation resulted in survival, differentiation, and site-specific integration of hMNPs distal to the SCI site within ventral horns, but hMNPs near the SCI site reverted to a neuronal progenitor state, suggesting an environmental deficiency for neuronal maturation associated with SCI. CONCLUSIONS/SIGNIFICANCE: These findings underscore the barriers imposed on neuronal differentiation of transplanted cells by the gliogenic nature of the injured spinal cord, and the physiological relevance of transplant-derived neurotrophic support to functional recovery.

Functional characterization of the chicken peptide transporter 1 (pept1, slc15a1) gene.

Dietary amino acids can be transported into intestinal epithelial cells as di- and tripeptides by the action of the peptide transporter, PepT1 (SLC15A1). Expression of the chicken PepT1 (cPepT1) gene changes in response to dietary crude protein level; however, the molecular mechanism governing this regulation is unknown. This study analyzed the promoter region of the cPepT1 gene. Using deletion analysis, positive-acting (-314 to -261, -169 to -155, and -120 to -60) and negative-acting (-419 to -386 and -214 to -169) regions were mapped in transfected chick embryo fibroblasts (CEF). The addition of neither amino acids Phe, Arg, or Val, nor the dipeptides Gly-Sar (glycyl-sarcosine), Gly-Pro, Gly-Phe, Met-Pro, Met-Lys or Lys-Lys, had an effect on cPepT1 promoter activity in transfected CEF. The cPepT1 promoter was more active in CEF and primary chicken intestinal cells than in chicken liver cells. This study represents a functional characterization of the molecular regulation of the chicken PepT1 gene.