Effect of life cycle and venation pattern on the coordination between stomatal and vein densities of herbs.

Life cycle (annual vs perennial) and leaf venation pattern (parallel and reticular) are known to be related to water use strategies in herb species and critical adaptation to certain climatic conditions. However, the effect of these two traits and how they influence the coordination between vein density (vein length per area, VLA) and stomatal density (SD) remains unclear. In this study, we examined the leaves of 53 herb species from a subtropical botanical garden in Guangdong Province, China, including herbs with different life cycles and leaf venation patterns. We assessed 21 leaf water-related functional traits for all species, including leaf area (LA), major and minor VLA, major and minor vein diameter (VD), SD and stomatal length (SL). The results showed no significant differences in mean SD and SL between either functional group (parallel venation vs reticular venation and annual vs perennial). However, parallel vein herbs and perennial herbs displayed a significantly higher mean LA and minor VD, and lower minor VLA compared to reticular vein herbs and annual herbs, respectively. There was a linear correlation between total VLA and SD in perennial and reticular vein herbs, but this kind of correlation was not found in annual and parallel vein herbs. The major VLA and minor VD were significantly affected by the interaction between life cycle and leaf venation pattern. Our findings suggested that VLA, rather than SD, may serve as a more adaptable structure regulated by herbaceous plants to support the coordination between leaf water supply and demand in the context of different life cycles and leaf venation patterns. The results of the present study provide mechanistic understandings of functional advantages of different leaf types, which may involve in species fitness in community assembly and divergent responses to climate changes.

Effect of leaf phenology and morphology on the coordination between stomatal and minor vein densities.

Leaf phenology (evergreen vs. deciduous) and morphology (simple vs. compound) are known to be related to water use strategies in tree species and critical adaptation to certain climatic conditions. However, the effect of these two traits and their interactions on the coordination between minor vein density (MVD) and stomatal density (SD) remains unclear. In this study, we examined the leaves of 108 tree species from plots in a primary subtropical forest in southern China, including tree species with different leaf morphologies and phenologies. We assessed nine leaf water-related functional traits for all species, including MVD, SD, leaf area (LA), minor vein thickness (MVT), and stomatal length (SL). The results showed no significant differences in mean LA and SD between either functional group (simple vs. compound and evergreen vs. deciduous). However, deciduous trees displayed a significantly higher mean MVD compared to evergreen trees. Similarly, compound-leaved trees have a higher (marginally significant) MVD than simple-leaved trees. Furthermore, we found that leaf morphology and phenology have significantly interactive effects on SL, and the compound-leafed deciduous trees exhibited the largest average SL among the four groups. There were significant correlations between the MVD and SD in all different tree groups; however, the slopes and interceptions differed within both morphology and phenology. Our results indicate that MVD, rather than SD, may be the more flexible structure for supporting the coordination between leaf water supply and demand in different leaf morphologies and phenologies. The results of the present study provide mechanistic understandings of the functional advantages of different leaf types, which may involve species fitness in community assembly and divergent responses to climate changes.

Spatial variability, source identification and risks assessment of antibiotics in multimedia of North China's largest freshwater lake using positive matrix factorization and Monte Carlo simulation.

Antibiotics are widely found in aquatic ecosystems and pose a serious threat to human and the ecological system. Samples of surface water (SW), overlying water (OW), pore water (PW) and sediments (Sedi) were collected to investigate the spatial variability, potential sources, ecological risk (RQs) and health risks (HQs) of nine common antibiotics in Baiyangdian Lake using positive matrix factorization (PMF), and Monte Carlo simulation. Significant spatial autocorrelation of most antibiotics were observed in PW and Sedi samples rather than in SW and OW samples, and higher antibiotic levels were found in the northwest of waters and the southwest of sediments. Livestock (26.74-35.57%) and aquaculture (21.62-37.70%) were identified as primary sources of antibiotics in the water and sediments. Norfloxacin and roxithromycin showed high levels of RQ and HQ in more than 50% of samples, respectively. The combined RQ (ΣRQ) in the PW can be used as a sign of across multimedia risk. Notably, appreciable health risks were observed for the combined HQ (ΣHQ) in about 80% of samples, indicating the importance of taking health risk of antibiotics into consideration. The findings of this work provides a reference for antibiotics pollution control and risk management in shallow lake.

Effects of microtopographic patterns on plant growth and soil improvement in coastal wetlands of the Yellow River Delta.

Introduction: To clarify the effects of microtopography on plant growth and soil water, salt and nutrient characteristics of saline soils in mudflats within muddy coastal zones and explore suitable microtopographic modifications. Methods: Six microtopographic modification patterns, namely, S-shaped, stripe-shaped, pin-shaped, stepshaped, dense stripe-shaped and crescent-shaped patterns, were established in the coastal mudflats of the Yellow River Delta. The soil water, salt, ion, total carbon, total nitrogen, and total phosphorus contents and their ecological stoichiometric characteristics were measured and analyzed after theimplementation of different microtopographic modification patterns, with bare mudflats as the control. Results: The results showed that microtopographic modification significantly changed the soil water and salt contents and the soil total carbon, total nitrogen and total phosphorus contents. Compared with the bare ground, microtopographic transformation significantly promoted the growth of the pioneer plant Suaeda salsa, significantly increased the soil water and nutrient contents, and significantly decreased the soil salinity. The soil salinity was mainly reduced by Na+ and Cl- ions. The soil salinity and nutrient contents gradually decreased with increasing soil depth, indicating the occurrence of surface aggregation. Compared to that of the bare ground, the soil C/N was significantly lower and the N/P was significantly higher in the microtopographic treatments, and the overall performance suggested soil N limitation. The ions contained in the saline soil were dominated by Na+ and Cl-, followed by Mg2+ and SO4 2-, with lower contents of K+, Ca2+ and HCO3 -. Among the six microtopography modification patterns, the crescent-shaped pattern best promoted vegetation restoration. This pattern was the most effective in reducing soil salinity, with a 98.53% reduction in soil salinity compared with that of bare ground, followed by the pin-shaped pattern. Compared with that in the bare ground samples, the nutrient content in the samples from the step-shaped modification increased by 23.27%; finally, the S-shaped, step-shaped and dense stripe-shaped patterns performed poorly in terms of plant restoration and soil improvement. Discussion: It is suggested that a crescent-shaped pattern should be considered first when carrying out microtopographic transformation on the beaches of the Yellow River Delta, followed by stripe-shaped and pin-shaped patterns. The dense strip-shaped should not be adopted.

Using Sweet Sorghum Varieties for the Phytoremediation of Petroleum-Contaminated Salinized Soil: A Preliminary Study Based on Pot Experiments.

Using energy plants to repair salinized soils polluted by petroleum is an efficient way to solve the problem of farmland reduction and prevent pollutants from entering the food chain simultaneously. In this study, pot experiments were conducted for the purposes of preliminarily discussing the potential of using an energy plant, sweet sorghum (Sorghum bicolor (L.) Moench), to repair petroleum-polluted salinized soils and obtain associated varieties with excellent remediation performance. The emergence rate, plant height and biomass of different varieties were measured to explore the performance of plants under petroleum pollution, and the removal of petroleum hydrocarbons in soil with candidate varieties was also studied. The results showed that the emergence rate of 24 of the 28 varieties were not reduced by the addition of 1.0 × 104 mg/kg petroleum in soils with a salinity of 0.31%. After a 40-day treatment in salinized soil with petroleum additions of 1.0 × 104 mg/kg, 4 potential well-performed varieties including Zhong Ketian No. 438, Ke Tian No. 24, Ke Tian No. 21 (KT21) and Ke Tian No. 6 with a plant height of >40 cm and dry weight of >4 g were screened. Obvious removal of petroleum hydrocarbons in the salinized soils planted with the four varieties were observed. Compared with the treatment without plants, the residual petroleum hydrocarbon concentrations in soils planted with KT21 decreased by 69.3%, 46.3%, 56.5%, 50.9% and 41.4%, for the additions of 0, 0.5 × 104, 1.0 × 104, 1.5 × 104 and 2.0 × 104 mg/kg, respectively. In general, KT21 had the best performance and application potential to remediate petroleum-polluted salinized soil.

[Soil organic carbon density and its influencing factors in croplands with different cultivation years in the Northeastern Ulan Buh Desert, China]. / ä¹Œå °å¸ƒå’Œæ²™æ¼ ä¸œåŒ—éƒ¨ä¸åŒè€•ä½œå¹´é™å†œç”°åœŸå£¤æœ‰æœºç¢³å¯†åº¦åŠå ¶å½±å“å› ç´ .

Understanding the changes and influencing factors of soil organic carbon density (SOCD) during the conversion of uncultivated natural soil to croplands is of great significance for the assessment of carbon sequestration in arid areas. In this study, we compared SOCD in the uncultivated soil and that in croplands with different cultivation years (2-5, 12-15, 25-30, 40-50 years) in the Northeastern Ulan Buh Desert. The change of SOCD and its influencing factors at 0-2 m soil depth during the conversion of uncultivated natural soil to croplands were explored by the method of replacing time with space. The results showed that SOCD at the shallow soil depth (0-0.4 m) in croplands increased continuously with cultivation years, but basically at low levels (0.990-1.983 kg·m-2). The SOCD at deep soil (1.2-2 m) increased in the croplands with longer cultivation years (25-30 and 40-50 years), whereas no obvious change trends in both the croplands with shorter cultivation years (2-5 and 12-15 years) and the uncultivated natural soil. The SOCD at deep soil (1.2-2 m) were relatively large (28.9%-38.6%) of the 0-2 m soil depth of uncultivated natural soil and croplands with different cultivation years. The vertical distribution of SOCD in croplands with different cultivation years were well fitted by quadratic functions (with R2 ranging from 0.757 to 0.972). It was noteworthy that soil clay and silt contents had dominant influences on SOCD at all the soil profile (0-2 m), and that cultivation years mainly contributed to the accumulation of SOC at the shallow soil (0-0.4 m).

Contrasting diversity patterns and community assembly mechanisms of bacterioplankton among different aquatic habitats in Lake Taihu, a large eutrophic shallow lake in China.

Eutrophication leads to the degradation of lake habitat types from macrophyte-dominated habitats (MDH) to algae-dominated habitats (ADH), which is a common environmental problem faced by many lakes. However, the variations in diversities and community assembly processes of bacterioplankton in the process of lake eutrophication have not been thoroughly elucidated. Here, we contrasted bacterial diversity patterns and processes of community assembly among ADH, MDH, and other habitats (OH) of Lake Taihu, a large shallow eutrophic lake in China with strong wind-induced disturbances. We found that the bacterial diversity patterns and potential functions between ADH and MDH were significantly different. Moreover, the contributions of purely environmental variables to the bacterial diversity patterns of all habitat types were much higher than those of spatial variables. However, the relative importance of stochasticity in the bacterial community assembly of each habitat type was much higher than that of determinism. Intriguingly, 'undominated' stochastic processes shape the diversity patterns of bacterioplankton in ADH, MDH, and OH of Lake Taihu. These findings demonstrate that the degradation of lake habitats caused by eutrophication can profoundly change the diversity and potential function patterns of the bacterioplankton community in lake ecosystems. Although the distinct diversity patterns of the bacterioplankton among the different aquatic habitats in Lake Taihu can be affected by deterministic processes (local environmental variables), they were dominated by stochastic processes (drift). Our study confirms that strong, disordered, wind-induced disturbances in shallow lakes could lead to strong hydrologic mixing, thus increasing the randomness of bacterial community assembly in each habitat.

Response of the fine root morphological and chemical traits of Tamarix chinensis to water and salt changes in coastal wetlands of the Yellow River Delta.

To explore the adaptation of the fine root morphology and chemical characteristics of Tamarix chinensis to water-salt heterogeneity in the groundwater-soil system of a coastal wetland zone, T. chinensis forests at different groundwater levels (high: GW1 0.54 m and GW2 0.83 m; medium: GW3 1.18 m; low: GW4 1.62 m and GW5 2.04 m) in the coastal wetland of the Yellow River Delta were researched, and the fine roots of T. chinensis standard trees were excavated. The fine roots were classified by the Pregitzer method, and the morphology, nutrients, and nonstructural carbohydrate characteristics of each order were determined. The results showed that the groundwater level had a significant indigenous effect on the soil water and salt conditions and affected the fine roots of T. chinensis. At high groundwater levels, the specific root length and specific surface area of fine roots were small, the root tissue density was high, the fine root growth rate was slow, the nutrient use efficiency was higher than at low groundwater levels, and the absorption of water increased with increasing specific surface area. With decreasing groundwater level, the N content and C/N ratio of fine roots first decreased and then increased, and the soluble sugar, starch, and nonstructural carbohydrate content of fine roots first increased and then decreased. At high and low groundwater levels, the metabolism of fine roots of T. chinensis was enhanced, and their adaptability to high salt content and low water content soil environments improved. The first- and second-order fine roots of T. chinensis were mainly responsible for water and nutrient absorption, while the higher-order (from the third to fifth orders) fine roots were primarily responsible for the transportation and storage of carbohydrates. The fine root morphology, nutrients, nonstructural carbohydrate characteristics, and other aspects of the water and salt environment heterogeneity cooperated in a synergistic response and trade-off adjustment.

Crab bioturbation affects competition between microbial nitrogen removal and retention in estuarine and coastal wetlands.

As the important benthic animal in coastal wetlands, crab bioturbation may significantly affect the nitrogen (N) budgets by regulating microbial N transformation processes. However, the response of interaction between different microbial N processes to crab bioturbation remains poorly understood. Here, a 30-day microcosmic experiment was conducted using sediment collected from the Yangtze Estuary wetland, followed by the determination of temporal variations of physicochemical parameters, N removal (denitrification plus anammox, which is defined as N2 production) and retention rates (nitrate dissimilatory reduction to ammonium, DNRA) as well as relevant gene abundances in response to different crabs densities. The results showed that crab bioturbation simultaneously promoted the rates of N2 production and DNRA processes. These two process rates were positively associated with the intensity of crab bioturbation, which was supported by molecular analysis of relevant functional gene abundance. Crab bioturbation was more beneficial to DNRA than N2 production. Due to this disproportionate stimulation, crab bioturbation increased the importance of DNRA, indicating that N retention was becoming more significant under crab bioturbation in estuarine and coastal wetlands. The variations of sediment total organic carbon and oxygen availability driven by crab bioturbation were the critical factors mediating the changes in microbial N removal and retention. Overall, our findings highlighted that crab bioturbation can affect the N budgets in estuarine and coastal wetlands by altering the competition between microbial N removal and retention.

The Effects of Secondary Growth of Spartina alterniflora after Treatment on Sediment Microorganisms in the Yellow River Delta.

As a typical invasive species, Spartina alterniflora is widely recognized as one of the primary threats to biodiversity in various habitats, including wetlands. Although the invasion by S. alterniflora has been managed in multiple ways, it may reappear after treatment. How S. alterniflora affects the soil microbial community in coastal wetlands during its regeneration process has not yet been clarified. Here, rhizosphere soil samples (RSPs) and bulk soil samples (SSPs) were collected in the S. alterniflora community and a high-throughput sequencing method was conducted to analyze the composition and diversity of soil microorganisms. Meanwhile, we also obtain the soil physicochemical properties. In the present study, there was no significant difference in the alpha diversity of both bacterial and fungal communities in the SSP and RSP groups. The PCoA (principal coordinate analysis) also showed that the microbial community structure did not differ significantly between the SSP and RSP groups. The results showed that except for pH, the total sulfur (TS) content, total nitrogen (TN) content, and electrical conductivity (EC) did not differ significantly (p > 0.05) between the SSP and RSP groups. The composition of the bacterial and fungal community in the rhizosphere of S. alterniflora was similar to that found in the surrounding soils. The top two dominant bacterial phyla were Proteobacteria and Desulfobacterota in the present study. Venn diagram results also support this view; most OTUs belong to the common OTUs of the two groups, and the proportion of unique OTUs is relatively small. The LEfSe (LDA effect size) analysis showed that Campylobacterota (at the phylum level) and Sulfurimonas (at the genus level) significantly increased in the RSP group, implying that the increased Sulfurimonas might play an essential role in the invasion by S. alterniflora during the under-water period. Overall, these results suggest that the bacterial and fungal communities were not significantly affected by the S. alterniflora invasion due to the short invasion time.

Patterns of compound-leaf form and deciduous-leaf habit across forests in China: Their association and key climatic factors.

Leaf form (compound vs. simple) and habit (evergreen vs. deciduous) are key functional traits of trees to adapt to various climates and are vital in determining plant response to climate change. However, their association and climatic determinants remain uncertain, especially in East Asian forests in the largest monsoon region on earth. To fill these knowledge gaps, we compiled a dataset comprising 42 intact forests and over 2200 angiosperm tree species across China (spanning 30 latitudes and 47 longitudes). The geographical and climatic patterns of leaf form and habit were analyzed. The association between compound leaf and deciduousness was tested for tropical, subtropical and temperate climatic zones. We found that both the percentage of compound leaf (CT%) and deciduous tree species (DT%) increased with latitude and decreased with mean annual precipitation (MAP). For all forests, DT% was negatively related to mean annual temperature (MAT), whereas CT% was not. Nevertheless, both DT% and CT% increased with increasing MAT in the tropics, possibly owing to the high vapor pressure deficits (VPD) and canopy water deficits associated with high temperatures. A positive linear relationship between CT% and DT% was found across all forests and within different climatic zones except for temperate, and the intercept of the regression line was significantly higher in the tropics than in the subtropics. Overall, as supported by principal component analysis, deciduousness was negatively associated with both temperature and precipitation, while CT negatively with precipitation only across zones and positively with temperature in the tropics. Different relationships in different climatic zones suggest potentially different selective forces. Our findings provide novel insights into the linkage between leaf form and habit, as well as how climate shapes the landscape of broadleaf forests, which has important implications regarding the response of forest composition to climate change.

Root growth and architecture of Tamarix chinensis in response to the groundwater level in the Yellow River Delta.

AIMS: Investigate the growth adaptation law of the Tamarix chinensis root system in response to the groundwater level in a muddy coastal zone. METHODS: The high groundwater level (0.7-0.9 m), medium groundwater level (1.1-1.3 m) and low groundwater level (1.5-1.7 m) T. chinensis forests on the beaches of the Yellow River Delta were used as the research objects. Full excavation methods were used to excavate root systems with different groundwater levels; then, the aboveground biomass, root biomass, root spatial distribution, root topological structure and fractal characteristics of T. chinensis response characteristics to groundwater level were measured and analysed. RESULTS: The results showed that with the decrease in the groundwater level, the soil water content and soil salt content showed upward trends. At high groundwater levels, T. chinensis reduced root biomass allocation to reduce the damage to roots caused by salinity. At low groundwater levels, T. chinensis strengthened the development of root systems, which greatly enhanced the ability of T. chinensis to balance its water intake. The root biomass at the high groundwater level was 43.06% lower than that at the low groundwater level. The relationship between root and shoot growth of T. chinensis at high groundwater levels and medium groundwater levels indicated allometric growth, and at low groundwater levels, roots and shoots grew uniformly. The root distribution of T. chinensis tended to be shallow at the different groundwater levels, showing the characteristics of a horizontal root type. At high groundwater levels, the root topological structure tended to be dichotomous, and the fractal dimension and fractal abundance values were both large, at 1.31 and 2.77, respectively. The branch complexity increased to achieve spatial expansion and increase plant stability. However, the topological structure of the medium and low groundwater level T. chinensis tended to be herringbone-like, the fractal dimension and fractal abundance values were small, the second branch was limited, and the structure was simple. The topological structure and fractal characteristics of the T. chinensis root system responded to different groundwater levels in a coordinated manner. CONCLUSIONS: Based on the differences in the growth and architecture of the T. chinensis root system, the T. chinensis root system has strong phenotypic plasticity to the heterogeneous water-salt habitat of the groundwater-soil system, and the T. chinensis root system shows strong root adaptability to water and salt stress.

Effects of different Tamarix chinensis-grass patterns on the soil quality of coastal saline soil in the Yellow River Delta, China.

Construction of circumlittoral shelter forest is of great significance to maintain ecological security of coastal zones, the safety of people's lives and property in the Yellow River Delta (YRD) in China. Tamarix chinensis-grass patterns have shown obvious advantages in construction of circumlittoral shelter forest and improving the soil quality of coastal saline soil. This study aimed to explore the soil-improving effects of various Tamarix chinensis-grass community patterns and identify the best vegetation pattern for improving the soil quality in the coastal saline-alkali land. Six kinds of Tamarix chinensis-grass community patterns were selected from the saline-alkali soil of the YRD, with bare land as the control. Effects of different Tamarix chinensis-grass patterns on the coastal saline soil were evaluated using statistical methods (e.g. principal component analysis and fuzzy membership function method). The results showed that various Tamarix chinensis-grass community patterns significantly decreased the salt contents and increased the available nutrient contents in the coastal saline-alkali soil. The soil improvement effects showed obvious distinctions among the different Tamarix chinensis-grass patterns. The mixed forest-grass pattern consisting of Tamarix chinensis, Phragmites australis, and other salt-resistant grasses showed the best effects in relation to reducing salt, preventing alkalization and increasing the soil nutrients, which resulted in the lowest salt contents and the highest nutrients. Grass species play a major role in increasing soil nutrient contents, and the density of new Tamarix chinensis forest contributes greatly to the decrease of soil salt. And the more kinds of grass species are, the better improvement effects they will have. Therefore, during the construction of the circumlittoral shelter forest system in the muddy coastal zone of the YRD, it is recommended to prioritize the high density Tamarix chinensis-Phragmites australis (TPA) community pattern, and live together with other kinds of salt-resistant grasses.

Diversity and functional characteristics of endophytic bacteria from two grass species growing on an oil-contaminated site in the Yellow River Delta, China.

Phragmites australis and Chloris virgata are native, dominant, salt-tolerant grass species that grow in the Yellow River Delta, China, and have potential applications in the phytoremediation of petroleum-polluted saline soil. The characteristics of endophytic bacterial communities of Phragmites australis and Chloris virgata and their functions in hydrocarbon degradation and plant growth promotion have been studied using both high-throughput sequencing and conventional microbial techniques. Through 16S rRNA gene amplicon sequencing, we found five bacterial phyla that were dominant among the endophytic bacterial communities of the two grass species, including Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, and Tenericutes. The phylum Proteobacteria was common among the endophytic bacterial communities of the two grass species. The diversity in the endophytic bacterial community of Chloris virgata was generally higher than that in the community of Phragmites australis. Thirty-eight hydrocarbon-degrading endophytic bacteria were isolated from the two grasses via culturing techniques. Based on phylogenetic analyses, the bacterial isolates were classified into the phyla Proteobacteria, Firmicutes, and Actinobacteria. The majority of strains belonged to the genera Bacillus and Pseudomonas. More than 70% of the isolates of hydrocarbon-degrading endophytes exhibited the ability to stimulate plant growth. These isolates mainly belonged to Bacillus sp., Pseudomonas sp., Beijerinckia sp., Serratia sp., Acinetobacter sp., Microbacterium sp., and Rhizobium sp. Altogether, the present study revealed that Phragmites australis and Chloris virgata growing on petroleum-polluted saline soil in the Yellow River Delta harbor several diverse species of endophytic bacteria and serve as novel sources of beneficial bacteria and hydrocarbon degradation.

[Research progress on soil aggregates and associated organic carbon in salinized soils]. / ç›æ¸åŒ–æ¡ä»¶ä¸‹åœŸå£¤å›¢èšä½“åŠå ¶æœ‰æœºç¢³ç ”ç©¶è¿›å±•.

Soil aggregate, as a basic component of soils, plays an important role in improving soil structure and enhancing soil organic carbon (SOC) sequestration. The special soil properties induced by salinization, such as high ion concentrations (mainly Na+), shortage of organic material and bad condition of microbe, inhibit the formation and stability of soil aggregate. Therefore, it is important and meaningful to explore the dynamics of aggregate in salinized soils. Coastal wetland and inland salinized marsh wetland are important salinized ecosystems. We systematically summarized the progress and achievements on soil aggregate in salinized agriculture and wetland ecosystems. Agricultural practices, such as organic and/or inorganic soil amendment application, tillage practice, vegetation type, straw return and saline water irrigation, advance the formation and stability of aggregate and aggregate-associated organic carbon in salinized soils. We discussed the problems and deficiency in the present studies of aggregate and aggregate-associated carbon in salinized soils as well as the research aspects and hot topics in the future. This review would be helpful for comprehensively understanding the advances and development directions on aggregate in salinized soils.

Photosynthetic characteristics of Tamarix chinensis under different groundwater depths in freshwater habitats.

Groundwater is the major source of water for Tamarix chinensis growth in the Yellow River Delta (YRD) region, and the groundwater depth (GWD) dramatically influences the physiological activities of T. chinensis. The quantitative response of the photosynthetic physiological process of T. chinensis to the GWD in freshwater habitats remains unclear. In this study, the response characteristics of gas exchange parameters in the leaves of three-year-old T. chinensis seedlings were measured and analyzed at a graded series of seven GWDs (0 m, 0.3 m, 0.6 m, 0.9 m, 1.2 m, 1.5 m and 1.8 m). The GWD thresholds corresponding to drastic changes in the photosynthetic efficiency and the GWDs of several levels of photosynthetic productivity and efficiency were also determined. In the freshwater habitats of the YRD, variations in GWD significantly altered the relative soil water content (RSWC) and thus influenced the photosynthetic efficiency of T. chinensis. RSWC at 0 ≤ GWD ≤ 0.9 m and GWD at 1.2 m ≤ GWD ≤ 1.8 m directly influenced the photosynthetic physiology of T. chinensis. When the GWD was 1.2 m, net photosynthetic rate (Pn), apparent quantum efficiency and water use efficiency (WUE) values all peaked. Thus, T. chinensis exhibited a high light and water use efficiency, wide ecological amplitude in terms of light, and high photosynthetic capacity. The optimum GWD for photosynthetic carbon assimilation and WUE in T. chinensis was determined to be 1.2 m. At a deep (≥1.64 m) or shallow (≤0.53 m) GWD, both Pn and WUE in T. chinensis clearly decreased below the corresponding mean values. The main causes for the reduction in Pn in these two GWD ranges (≤0.53 m, ≥1.64 m) were stomatal and nonstomatal limitations, respectively. Additionally, a moderate GWD of 1.09-1.25 m corresponded to the "high-productivity and high-efficiency GWD" range, in which T. chinensis displayed a high photosynthetic yield and WUE. Overall, the photosynthetic capacity of T. chinensis shows characteristics of high tolerance to moderate GWDs from 1.09 m to 1.25 m but intolerance at both shallow (≤0.53 m) and deep (≥1.64 m) GWDs in freshwater habitats.

Elemental stoichiometry (C, N, P) of soil in the Yellow River Delta nature reserve: Understanding N and P status of soil in the coastal estuary.

The Yellow River Delta Nature Reserve (YNR), which includes two separated regions: part of the old Yellow River Delta (OYD) and part of the current Yellow River Delta (CYD), was established to protect coastal wetlands in the coastal estuary. A total of 120 plots were sampled in the YNR in April 2016, and the spatial patterns of soil C, N and P contents and their stoichiometric ratios (C:N (RCN), C:P (RCP) and N:P (RNP)) were studied and interpolated using the Ordinary Kriging method. Results indicated that the soil elemental contents and stoichiometric ratios showed high spatial heterogeneity and large variations. The mean C:N:P ratio (RCNP) was ~ 64.7:2.3:1 in OYD, and ~ 64.5:2.0:1 in CYD, respectively, and a well-constrained RCP ratio ~ 65:1 was found in the 0-50 cm soil depth within the YNR. N showed greater variation than C and P. Furthermore, N contents in the 0-5 cm soil layer of OYD were significantly higher than that of CYD (F = 4.79, p = 0.03); RCN in 0-5 cm, 5-10 cm layers of OYD was significantly lower than those in the same layers of CYD (F = 4.75, p = 0.03; F = 5.18, p = 0.02, respectively). RNP in 0-5 cm soil layer of OYD was notably higher than that of CYD (F = 4.88, p = 0.03). These results were due to the combined actions of sedimentation, reclamation and fertilization. Finally, we concluded that a longer reclamation and fertilization history led to decreased RCN in coastal estuary soils, confirmed that the soil of the YNR exhibits N limitation, and suggested that the soil RCN and RNP could be good indicators of the anthropogenic improvement status during soil development in this coastal estuary.

Biochar and effective microorganisms promote Sesbania cannabina growth and soil quality in the coastal saline-alkali soil of the Yellow River Delta, China.

Soil salinization and nutrient deficiency have emerged as the major factors negatively impacting soil quality and primary productivity in the coastal saline-alkali soil of the Yellow River Delta. Biochar has been proposed as an efficient strategy for promoting plant growth and restoring degraded saline-alkali soil. However, knowledge is inadequate regarding the effects of adding Spartina alterniflora-derived biochar alone or in combination with effective microorganisms (EM) on the growth of Sesbania cannabina and soil quality in saline-alkali soil. To enhance this knowledge, a pot experiment with different EM treatments (without EM addition, EM-; with EM addition, EM+) and a gradient of biochar treatments (0%, B0; 0.5%, B1; 1.5%, B2; and 3%, B3; biochar weight/soil weight) was conducted. Our results showed that biochar addition alone and in combination with EM significantly increased seed germination, plant height, stem diameter, total biomass and plant nutrient uptake of S. cannabina. Biochar addition, EM addition and their interaction significantly decreased soil salt content efficiently and increased soil total carbon (TC), total nitrogen (TN), available phosphorus (AP) and available potassium (AK) but had little effect on soil pH. Biochar addition increased soil organic carbon, soil NH4+ and NO3-, microbial biomass carbon, and soil enzyme activities and these effects increased in strength when biochar and EM were present simultaneously. Of the treatments, the EM + B3 treatment had the largest effects in terms of inhibiting salinization, increasing soil fertility, elevating soil nutrients and enzyme activities, and improving plant growth. Moreover, the application of biochar and EM promoted the growth of S. cannabina by enhancing plant nutrient uptake, improving soil fertility (e.g., TN, AP, AK, NH4+ and NO3-), and elevating soil enzyme activities (urease and alkaline phosphatase activity). Overall, the integrated use of an appropriate biochar rate (3%) and EM for coastal saline-alkali soil could be an effective strategy to ameliorate soil salinity, improve soil quality and promote plant productivity.

[Effects of biochar and EM application on growth and photosynthetic characteristics of Sesbania cannabina in saline-alkali soil of the Yellow River Delta, China]. / 生物炭和EMèŒå¯¹é»„æ²³ä¸‰è§’æ´²ç›ç¢±åœ°ç”°èç”Ÿé•¿å’Œå ‰åˆç‰¹æ€§çš„å½±å“.

We examined the effects of biochar and effective mircoorganisms (EM) application on growth and photosynthetic characteristics of Sesbania cannabina in the Yellow River Delta, by a pot experiment with different EM treatments (without EM addition, EM-; with EM addition, EM+) and a gradient of biochar treatments (0, B0; 0.5%, B1; 1.5%, B2; 3%, B3; biochar weight/soil weight). The growth parameters, photosynthetic light response curve and chlorophyll fluorescence characteristics of S. cannabina were measured. The results showed that the EM+B3 treatment had the best effect among all the treatments. Compared with the EM-B0 treatment, the EM+B3 treatment increased height, stem diameter, and total biomass by 69.5%, 90.0% and 141.1%, respectively. Biochar and EM significantly improved photosynthetic capacity. Compared with the EM-B0 treatment, the EM+B3 treatment significantly enhanced the maximum light response of net photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance by 93.8%, 35.1%, 43.4%, and 34.8%, respectively. Biochar and EM improved the parameters of chlorophyll fluorescence. Compared with the EM-B0 treatment, the EM+B3 treatment significantly increased the potential photochemical efficiency, the actual photochemical efficiency, the apparent electron transport rate and the non-photochemical quenching coefficient by 25.8%, 31.5%, 37.2%, and 56.8%, respectively. The parameters of growth, photosynthesis and chlorophyll fluorescence increased with the increasing biochar under EM+ treatments, whereas the B3 treatment had negative effect under EM- treatments. The co-addition of EM and 3% biochar (EM+B3) could improve the photosynthetic capacity and chlorophyll fluorescence characteristics of S. cannabina, broaden light ecological amplitude, boost the water retention and drought resistance property, and promote the growth of S. cannabina.

Physiological and ecological characteristics of Periploca sepium Bunge under drought stress on shell sand in the Yellow River Delta of China.

This study investigated the physiological and ecological changes in P. sepium Bunge and elucidated the physiological regulatory mechanisms underlying the adaptation of P. sepium to drought stress in shell sand. Drought stress led to a significant decrease in the net photosynthesis rate (Pn) and respiration rate of leaves and a decrease in low-intensity light-use efficiency (LUE) and light ecological amplitude. An increase in drought stress led to a considerable decrease in the photosynthetic electron transport rate in the P. sepium leaves and a significant increase in the amount of light energy dissipated as heat. In addition, the photosynthesis process suffered from severe photoinhibition. P. sepium plants counteracted the effects of drought stress primarily by increasing their peroxidase (POD) activity and by regulating membrane lipid peroxidation by secreting greater numbers of osmotic adjustment substances (proline (Pro) and soluble sugars (Ss)) and malondialdehyde (MDA). As drought stress increased, both the stem sap flow rate and the cumulative sap flow of P. sepium decreased considerably. P. sepium Bunge adapts to drought stress through interregulatory activity between photosynthesis, water-related physiological activities, and physiological and biochemical processes, and this species exhibits relatively high adaptive plasticity to drought.